Objective To evaluate the significance of p53 protein expression of hepatocellular carcinoma (HCCs) cells after transcatheter arterial chemoembolization(TACE). Methods A total of 136 patients with HCC underwent liver resection. One to five courses of TACE prior to liver resection were performed in 79 patients (TACE group), in which one to four courses of chemotherapy alone were performed in 11 patients (Group A), one to five courses of chemotherapy combined with iodized oil were performed in 33 patients (Group B), one to three courses of chemotherapy combined with iodized oil and gelatin sponge were performed in 23 patients (Group C), and one to three courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge were performed in 12 patients (Group D). The other 57 patients only received liver resection (non-TACE group). The extent of apoptosis was analyzed by transferase-mediated dUTP nick end labeling (TUNEL) staining. The expressions of Bcl-2, Bax, Ki-67 and PCNA proteins were detected by immunohistochemical method. Results p53 protein expressions of trabecular and clear cell HCCs were significantly lower than those of pseudoglandular, solid, poorly differentiated or undifferentiated and sclerosis HCCs (P

Objective To explore the effect of heterosexual interaction training on rehabilitation in first-episode schizophrenic patients. Methods 246 patients of first-episode schizophrenic were divided into the study group and the control group with 123 cases in each group at random. Both groups were treat-ed with conventional pharmacotherapy under the orders of physicians in hospital. For the study group,both the male patients and the female patients took part in entertainment treatment activities,eating and interaction training together during the day, then they lived separately at night. Two groups were evaluated with BPRS and ITAQ before treatment and at the 2nd, 4th, 6th, and 8th week after treatment. Results The BPRS scores of the study group were significantly lower than the control group after 4th, 6th,and 8th week. The ITAQ scores of the study group were significantly higher than the control group after 4th, 6th,and 8th week. Conclusions The application of heterosexual interaction training in schizophrenic patients can alleviate illness condition, improve treatment compliance and rehabilitation.

Objective　To evaluate the intelligence level and different subscales in TS patients. Methods　64 patients with TS and 60 normal children were evaluated with C-WISC. Results　The intelligence quotient of most TS patients fluctuated between normal and borderline range, all subscales except Vocabulary and knowledge, PIQ, VIQ and FIQ were significantly lower in TS patients than in controls (P＜0.05), the balance between PIQ and VIQ was poor in TS patients. Conclusion　The intelligent quotient of TS patients was lower than that of normal children, TS patients had more imbalance in intelligence development.

Objective To study the body composition of public security staffs and to prevent sub-health condition positively. Methods Body compositions of 771 public security staffs were measured and analyzed with bioelectrical impedance analytic process (BIA). Results The male public security staffs had obviously lower body fat percentage (P< 0.01) but higher body mass index and obesity (P<0.05) than female public security staffs. Both male and female public security staffs fad the highest content of fat in the lower limbs. The body part ranked the second place, followed by the upper limbs. Measured by body-fat percentage(BF%), the obesity rate of the male and female staffs was 10.08% and 45.59% respectively. Measured by body mass index (BMI), the hypergravity rate of the male and female staffs was 44.09% and 20.59% respectively; and the obesity rate of the male and female staffs was 8.35% and 0.74% respectively. Measured by obesity degree (OBD), the hypergravity rate of the male and female staffs was 21.73% and 4.41% respectively; and the obesity rate of the male and female staffs was 11.65% and 1.47% respectively. Conclusion Public security staffs should pay attention to their over-weight and hyper-fat level. Proper dict and body exercise are needed.

Objective To investigate a periosteal autograft method to repair major defects of joint cartilage in rabbits of different ages. Methods Major cartilage defects of 52 rabbits aged 4 to 52 weeks were repaired with free periosteal grafts and the results were compared in the study. The regenerated cartilage by the cambium of periosteum growing up to the cavity of joint and that growing down to the cancellus of femur were also compared. Results The macroscopical and histological observations at different intervals showed that in young and adult rabbits the grafts could develop into cartilages to repair the major cartilage defects. In adult rabbits the newly formed cartilage was the same as the normal cartilage in thickness and histology. There was no difference between the cambium growing up to the cavity and that growing down to the cancellus. Conclusions Periosteum possesses chondrogenic potential and can be used to repair the cartilage defects. Different directions of chondrogenesis leads to no significantly different outcomes. In adult rabbits the newly formed cartilage can develop into a normal one.

Objective To discuss the safety and feasibility of percutaneous transhepatic biliary preset implantation of after-loading radiotherapy catheter in treating bile duct carcinoma of ampulla.Methods A total of 10 patients with carcinoma of ampulla were collected.After percutaneous transhepatic biliary stent implantation was accomplished,the after-loading radiotherapy catheter was inserted via the guide wire.Based on the lesion's location,the positioning of both the catheter tip and the simulation radioactive source implanted through catheter was conducted.Each time before irradiation,the positions of the catheter and the radioactive source were reset under CT or fluoroscopic guidance in order to ensure that the lesion could get adequate internal irradiation dose.Results Percutaneous transhepatic biliary implantation of after-loading radiotherapy catheter and the positioning of simulation radioactive source were successfully accomplished in all 10 patients,and the total internal irradiation dose was completed within 5-7 days after catheter implantation.No severe complications occurred during the whole therapeutic process.Conclusion The technology of percutaneous transhepatic biliary preset implantation of after-loading radiotherapy catheter is safe and feasible,it carries high success rate with less complications.This therapy can improve the patency rate of biliary stent,and,as a palliative treatment,it is suitable for patients with carcinoma of ampulla.This treatment is worthy of application and promotion.

Objective To observe the effects of puhnonary rehabilitation on the symptoms and quality of life of persons with chronic obstructive pulmonary disease (COPD).Methods COPD patients were recruited from a hospital and community health centers and given pulmonary rehabilitation in the community.The rehabilitation program included health education and nutrition guidance,medication,breathing exercises,and physical exercise.Each patient was followed up every 4 weeks by telephone.The outcome measures included pulmonary function (FEV1％,FEV1/FVC％),St George's questionnaire (SGRQ),the Medical Research Council scale (MRC),Borg scoring,evaluation of anxiety and depression,the six-minute walk test (6MWT),and an evaluation of nutritional status.All of the patients were observed for one year.Results A total of 85 patients completed the program and were included in the final statistical analysis.There was no difference in FEV1 ％ before and after rehabilitation.Six-minute walking distance improved significantly in statistical terms but the average increase was only from (416.1 ±99.84)m to (437.51 ±116.17)m.The SGRQ scores decreased by an average of 4.8 points after a year of rehabilitation,with significant improvements among the moderately and severely impaired.Incidents of COPD exacerbation also decreased significantly.Patients with a low body mass index had the greatest SGRQ score improvments.Few patients showed depression or anxiety in this research population.Conclusions Pulmonary rehabilitation in the community can improve exercise tolerance and the quality of life for persons with COPD,reducing incidents of acute exacerbation.Patients may benefit from such pulmonary rehabilitation regardless of disease severity.The compliance was good despite the infrequent monitoring.Pulmonary rehabilitation in the community is worthy of more widespread use.

Aim To observe the optimal temperature and optimal pH of uricase-catalase liposomes(UCALP) and free uricase(UAE), and study the abilities of UCALP to reduce uric acid and hydrogen peroxide in mice with hyperuricemia.Methods UCALP were prepared by reverse phase evaporation, optimal temperature and optimal pH of UCALP and UAE were determined, respectively.Mouse model of hyperuricemia was established by intraperitoneally injection of uric acid, and the model mice were intravenously injected UCALP and UAE, respectively, then the serum concentration of uric acid and hydrogen peroxide in mice at different time points were measured by the assay kits, respectively.Results Optimal temperature of UCALP and UAE was 40℃, and optimal pH was 8.0 and 8.5, respectively.UCALP could more significantly lower uric acid level of hyperuricemia mice than that of UAE, and the concentration of hydrogen peroxide in UCALP group was lower than in UAE group.Conclusion UCALP can effectively decrease the level of uric acid and control the level of hydrogen peroxide in mice with hyperuricemia.

Aim To prepare the novel pyridostigmine bromide nanoemusion(PPNE)and study its release in vitro, and to investigate the intestinal absorption. Methods Pyridostigmine bromide (PB)and PPNE were tested by HPLC in pH 1 .2 HCl,pH 6.8,pH 7.4,pH 7.8 PBS.Rat single pass intestinal perfusion method was employed to investigate the absorption mechanism of PB and PPNE.Results PB release rate was faster than PB in the four release media;the intes-tinal absorption rate constant(Ka )and apparent perme-ability coefficient(Papp)of PPNE were increased in the duodenum,jejunum,ileum and colon segments.PB and PPNE had significant difference in the duodenum, jejunum,ileum and colon segments by t test (P <0.05).Conclusions PPNE can improve the bioavail-ability of drugs,increase the drugs permeability,sig-nificantly improve the absorption of the drugs in the in-testinal segments. PPNE has obviously sustained effects.

Pulmonary fibrosis is a common respiratory disease in the clinic. Until now, the pathogenesis is still un-clear. Using clotting mechanism as the starting point, this article mainly explored abnormal changes of the coagula-tion - fibrinolysis system in the development of pulmonary fibrosis. The effective treatment through the activation of blood circulation to remove stasis in traditional Chinese medicine (TCM) point of view was also observed on the man-agement of interstitial pulmonary fibrosis. It considered that to carry out the anticoagulant therapy for abnormal coag-ulation, which may become a new target for clinical treatment of interstitial lung diseases. It provided new ideas and theoretical support for clinical treatment of pulmonary interstitial fibrosis.