Objective To analyze the genotype and plasmid transfer of Enterobacteriaceae carring blaNDM‐1 with blaIMP‐4 or blaKPC‐2 .Methods From April 2012 to October 2014 ,a total of 33 non‐repeatitive carbapenem‐resistant Enterobacteriaceae ( including Imipenem‐resistant , meropenem‐resistant or Ertapenem‐resistant) were isolated from 5 hospitals in Wenzhou and Hangzhou . Identification and antimicrobial susceptibility test were performed using Vitek 2 Compact automatic microbiology analyzer . Phenotypes of carbapenemase were screened using modified Hodge test and ethylenediamine tetraacetic acid‐disk synergy test .Extended spectrum βlactamase test was determined by the double disk combination test which was recommended by Clinical and Laboratory Standards Institute .AmpC activity was tested by a three‐dimensional Cefoxitin method .Drug resistant genes including blaNDM‐1 and linkage of ISAba125‐NDM were detected by polymerase chain reaction (PCR) .The purified PCR products were cloned and sequenced .Plasmid conjugation experiment and elimination method were carried out to test partial bacterial strain and K . pneumoniae carrying blaNDM‐1 with blaIMP‐4 or blaKPC‐2 .Results Of the 33 non‐repeatitive carbapenem‐resistant Enterobacteriaceae ,28 were strains of K .pneumoniae ,1 strain of K . oxytoca,2strainsof Escherichiacoli,1strainof K.planticolaand1strainof E.cloacae.Thirteenstrains were isolated from Hospital of Sir Run Run Shaw of Zhejiang University ,thirteen from Wenzhou Hospital of Traditional Chinese Medicine ,one from Wenzhou People′s Hospital ,three from the First Affiliated Hospital of Wenzhou Medical University and three from Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine .Thirty‐one strains were confirmed as carbapenemase‐producing with 24 of blaKPC‐2 ,3 of blaNDM‐1 ,1 of blaNDM‐5 and 3 of blaIMP‐4 .Among them ,one strain carried blaNDM‐1 with blaIMP‐4 and one strain carried blaNDM‐1with blaKPC‐2 ,respectively .The plasmid transfer and conjugation experiment was performed between strains carrying blaNDM‐1 and Escherichia coli EC600 or K . pneumoniae ATCC13833 and genes of blaNDM‐1 and ISAba125‐NDM were obtained .Conclusions blaKPC‐2 gene is the popular carbapenemase genotype .blaNDM‐1 or blaNDM‐5 may be correlated with linkage gene of ISAba125‐N DM .Coexistence of blaNDM‐1 carrying blaIMP‐4 or blaKPC‐2 is detected in the same strain , respectively . Enough importance should be attached to the strains ,because most of them are multiple drug resistance with related genes located in the plasmid which is easily spread between strains .

This article introduced the clinical experience of national famous TCM doctor Professor Xu Xin in the treatment of blood withered menstrual closure combined with obstruction in the abdomen.Professor Xu Xin believes that premature ovarian failure belongs to the category of blood withered menstrual closure in TCM,the etiology of which can be seen in essence and blood depletion and emotional anomalies;the mechanism of the disease is closely related to the dysfunction of visceral organs,and the source of qi and blood is not available,and the lack of essence and blood of the Chong Ren cystic uterus.Therefore,the treatment should be based on the basic rules of tonifying essence,qi and blood,adjusting the viscera and organs,and promoting blood circulation,and regulating menstruation.If combined with the obstruction in the abdomen,the patients'syndrome of blood stasis will be highlighted,and it is necessary to pay attention to the choice of drugs for removing blood stasis at the same time of applying the basic treatment,so as to support the healthy qi of the disease without leaving blood stasis,eliminating the obstruction in the abdomen without harming the healthy qi of the disease,and regulating the qi and blood to balance yin and yang,in order to obtain a significant therapeutic effect.

Objective Based on the data of chronic diseases and nutrition surveillance among Chinese adults in 2015,dyslipidemia and related factors were analyzed.Methods Multi-stage stratified cluster random sampling was used to select participants who were aged 18 and over,with questionnaire survey and related measurements conducted.Prevalence rates of dyslipidemia among participants by different characteristics and influencing factors were analyzed,using the method of complex weighting and post-weighted stratification.Results In all,7 404 participants were included.The overall prevalence of dyslipidemia was 30.5％ among the adults.The overall prevalence of dyslipidemia were 36.5％ in males and 24.4％ in females (P＜0.05).The prevalence rates of hypercholesterolemia,hypertriglyceridemia,high LDL-C,and low HDL-C were 3.7％,12.2％,5.3％,and 19.4％,respectively.Results from the multivariate logistic regression model analysis showed that age (OR=1.009,95％CI:1.000-1.018),female (OR=0.501,95％CI:0.397-0.632),College degree or above (OR=1.728,95％CI:1.257-2.374),alcohol consumption 3 (OR=0.711,95％CI:0.536-0.943),central obesity (OR=1.868,95％CI:1.547-2.257),BMI (OR=1.141,95％CI:1.098-1.186),hypertension (OR=1.259,95％CI:1.077-1.473) and diabetes (OR=2.025,95％CI:1.446-2.835) were influencing factors on dyslipidemia.Conclusions The prevalence of dyslipidemia seemed high among adults in Anhui.Risk factors should be closely monitored and under control,including those people with unhealthy lifestyles or being overweight,obesity,hypertensive and diabetic.

Objective:To explore the distribution of different subsets of monocyte in peripheral blood of patients with thyroid-associated ophthalmopathy (TAO).Methods:A cross-sectional study was performed.Fifty-nine TAO patients and 30 healthy subjects were recruited continuously in Zhongshan Ophthalmic Center from January 2017 to December 2019.Clinical data of subjects were recorded, and the severity and activity of TAO were graded based on the criteria of NOSPECS and CAS.TAO patients were grouped according to clinical activity of TAO, and the patients were treated by triamcinolone acetonide (TA) injection or methylprednisolone pulse therapy (MPT) accordingly.Peripheral blood of the subjects was collected and monocytes were isolated.The proportion of different monocyte subsets was assayed by a flow cytometry.The differences in distribution of monocyte subsets between TAO group and normal control group, stable TAO group and active TAO group, TA injected group and MPT treated group were compared and analyzed.The study protocol was approved by the Ethics Committee of Zhongshan Ophthalmic Center, Sun Yat-Sen University (No.2014MEKY005), and the written informed consent was obtained from each subject before any medical intervention.Results:The proportion of classical monocyte (CMo) subset in TAO group was (81.77%±5.53)%, which was significantly lower than (84.35±5.83)% in the normal control group ( P=0.034); the proportion of intermediate monocyte (IMo) subset in the TAO group was (10.17±4.19)%, which was significantly higher than (7.69±4.09)% in the normal control group ( P=0.006); no significant difference was found in the proportion of non-classical monocyte (NMo) subset between the two groups ( P=0.892). The proportion of CMo subset in the active TAO group was (77.29±5.80)%, which was significantly lower than (82.64±5.03)% in the stable TAO group ( P<0.01), and the proportion of IMo subset in the active TAO group was (13.79±4.82)%, which was significantly higher than (9.20±3.56)% in the stable TAO group ( P<0.01); no significant change was found in the proportion of NMo subset between the two groups ( P=0.283). There was no difference in the proportion of different TAO subsets before and after TA injection ( P>0.05). In MPT treated group, the proportion of CMo subset in TAO patients was significantly increased and the proportion of IMo subset was significantly decreased (both at P<0.05); there was no significant difference in proportion of NMo subset before and after MPT treatment ( P=0.187). Conclusions:IMo subset is enriched in patients with TAO, and the IMo subset content varies over the disease activity.MPT may inhibit the shift of CMo subset towards IMo subset.

Objective:To investigate the prevalence of metabolic syndrome(MS) among adult residents with different characteristics and the relationship between serum uric acid(SUA) level and MS using the data of Chinese Adult Chronic Diseases and Nutrition Surveillance(2018) program in Anhui.Methods:Multi-stage stratified cluster random sampling was used to select participants aged 18 and over for questionnaires, physical measurements, and laboratory tests. The complex weighted method was used to estimate the prevalence of MS among residents with different characteristics. Logistic regression model based on complex sampling data was used to analyze the relationship between SUA and MS. Receiver operating characteristic(ROC) curve was used to evaluate the reliability of SUA in diagnosing MS and determine the optimal cutoff point.Results:A total of 7 182 participants were included and the prevalence of MS among adult residents was 29.46%. The prevalence of MS was higher in females(33.76%) than that in males(25.28%), and the difference was statistically significant( P<0.05). After adjusting for other factors, for every 10 μmol/L increase in SUA, the risk of MS increased by 4% in males( OR=1.040, 95% CI 1.019-1.061) and 7% in females( OR=1.070, 95% CI 1.059-1.082). The area under the curve(AUC) for SUA in diagnosing MS was 0.816(95% CI 0.806-0.826), with a sensitivity of 0.761 and specificity of 0.727. The optimal cutoff point for SUA was 450 μmol/L. Conclusion:The prevalence of MS among adult residents in Anhui Province is 29.46%. SUA is a risk factor for MS, and increasing SUA level indicated a higher risk of MS. The optimal cutoff value of SUA may be helpful in diagnosing MS.

Objective:Based on the data of the baseline survey of hypertension and sodium intake monitoring in Anhui province in 2019, the salt intake in adult residents was estimated.Methods:Multi-stage stratified cluster random sampling was used to select participants aged 18-69 years, questionnaire survey and related measurements were conducted. Salt intake in participants with different characteristics were estimated with complex sample and linearization of Taylor series based on design and the correlation between salt intake and blood pressure, waist circumference and BMI were tested by linear regression.Results:A total of 1 500 participants were included. The overall salt intake was 9.14 g/d, which was 9.84 g/d in men and 8.47 g/d in women ( P<0.05). The differences in salt intake across different subgroups were significant ( P<0.05). Univariate linear regression analysis showed that salt intake was positively correlated with SBP, DBP, waist circumference and BMI ( P<0.05), while multivariate linear regression analysis (adjusted for other factors) only showed a positive correlation between salt intake and BMI ( β=0.053,95% CI: 0.028-0.078, P<0.05). Conclusion:The dietary salt intake in adult residents in Anhui was higher than WHO recommendation, suggesting that public health education need to be taken to reduce salt intake.

In modern medicine,digestive diseases and gynecological diseases belong to different disciplines.From a holistic perspective of TCM,the combination of the two can be collectively referred to as Dai Meridian disease.TCM believes that the basic etiology and pathogenesis of this disease are the obstruction damp heat,the spleen obstruction of cold and dampness,the burning of fire and heat,and the deficiency of essence,qi,and blood causing the deficiency and depression of the meridian.The treatment mainly adopts the method of regulating Dai Meridian,which is a manifestation of the overall differentiation and treatment of TCM,by referring to the Dai Meridian,spleen,stomach,liver,and Thoroughfare Vessel and Conception Vessel.The method of regulating Dai Meridian includes clearing dampness and heat in the meridian,cooling and dampness in the warm meridian,relieving pain in the meridian,and strengthening the meridian deficiency.When treating abdominal symptoms including digestive and gynecological diseases in clinical practice,it is advisable to consider and flexibly apply it,simplify complexity,and with homotherapy for heteropathy.