[Objective] To evaluate the diagnostic value of the recombinant antigen of 39 amino acid repeats encoded by a kinesin-like gene of Leishmania changasi (rK39) in serodiagnosis of visceral leishmaniasis (VL). [Methods] In Kashi, Xinjiang, 13 VL patients with splenomegaly and bone marrow aspirate culture positive were subjected to dipstick assay. A drop of whole blood or serum from patient was placed at the absorbing pad at the bottom of the dipstick.Flooding of the bottom protein with buffer allows serum proteins to migrate upwards, producing the positive band and Western blot analysis of rK39 subsequently performed with the sera collected. [Results] The end-point titers of antirK39 antibodies of these sera were determined by ELISA and found to fall within the range of 10-2 to 10-4, which were consistent with the intensity of their reaction with rK39 in dipstick assay. The positive sera could also recognize the specific rK39 band as analyzed by Western blot analysis. [Conclusion] The rK39 dipstick assay is more rapid, specific, sensitive and less invasive than the conventional methods of diagnosis for VL in the areas of low endemicity.

目的观察太极拳对出院后老年冠状动脉性心脏病(冠心病)患者的康复训练效果。方法24例老年冠心病患者出院后,进行二十四式太极拳训练,每天1次,观察3个月。结果经过3个月康复训练,患者的舒张压降低,1分钟储备心率改善(P<0.05)。结论太极拳有利于老年冠心病患者的心脏功能康复。

Objective To investigate and evaluate serum level of heparanase in diabetic patients with acute coronary syndrome.Methods Forty-six diabetic patients with acute coronary syndrome(ACS) were enrolled as DM+ACS group,as well as,52 patients with ACS were studied as ACS group,27 diabetic patients as DM group,and 28 health volunteers served as normal control group.CRP,MMP-9 and heparanase in serum were measured with ELISA.The receiver operating characteristic curves of CRP,MMP-9 and heparanase were produced and the cutoff values were determined.Results The serum level of heparanase was significantly higher in DM+ACS group than in the ACS group,DM group and normal control group.The areas under the curve(AUC) of ROC were 0.941,0.813 and 0.967 respectively.Significant difference of AUC were observed between groups.The serum level of heparanase was significantly higher in DM+AMI subgroup than in DM+UAP subgroup.Conclusion Serum heparanase is related with the development of acute coronary syndrome in diabetic patients.Serum heparanase can be used as a more effective serological indicator for the plaque destabilization and rypture in diabetic patients than CRP and MMP-9.

Acupuncture Points ; Adolescent ; Adult ; Electroacupuncture ; Female ; Humans ; Male ; Middle Aged ; Spinal Cord Injuries ; complications ; Urinary Bladder, Neurogenic ; etiology ; physiopathology ; therapy ; Urination ; Young Adult

0.05). Conclusion This method is sensitive, accurate, stabile and is applicable to the determination of inorganic arsenic in groundwater samples.

Objective To know the distribution of arsenic and trace elements in drinking water in the endemic arsenism areas in Shanyin County, Shanxi Province. Methods Drinking water samples were collected from the chronic endemic arsenic poisoning areas of Silizhuang and Shanghexi, Shanyin County, Shanxi Province and HPLC-HG-AFS system was used to analyze the arsenic contents, valence and speciation. The trace elements were determined by ICP-MS. The data were analyzed by SPSS13.0 for windows. Results A total of 63 drinking water samples were determined, 39.68% of them exceeded the standard limit of arsenic level, inorganic arsenic was the main arsenical in drinking water, iAs3+/(iAs3++iAs5+) ratio was 56.17% in the water sample (arsenic was higher than 50 ?g/L) . The contents of Fe, Mn, Hg, Se and Pb in part of drinking water samples exceed the standard limits. No significant difference was seen between the arsenic results determined by HPLC-HG-AFS and ICP-MS. Conclusion Arsenic contamination of drinking water in Shanyin County, Shanxi Province is severe. The most frequently seen arsenic valence state in drinking water is iAs3+. Some trace elements in part of drinking water samples exceed the standard limits, this may aggravate arsenic poisoning.

Objective To investigate the culture and proliferation of dendritic cells from human cord blood and malignant pleural effusions and identify the phenotype and the function of them in vitro. Methods Existing DCs were obtained from cord blood and malignant pleural effusions. The DCs were cultured with IL-4, GM-CSF and TNF-?. The morphological properties of DCs were observed by invert optical microscope. The phenotypes of DCs were analysed by flow cytometry. The proliferative bioactivities of T cells activated by DCs were observed with mixed lymphocyte reaction (MLR). Results No remarkable differences were found in the morphology and cellular phenotype of the DC, derived from two different sources, but there was remarkable difference between their culture time. The DCs from two different sources could activate homologous lymphocytes to proliferate mostly. But the capacity to stimulate the proliferation of homologous lymphocytes had remarkable difference. Conclusion Mature DCs could be induced from cord blood and malignant pleural effusions.

Objective To explore the feasibility of individualized preoperative preparation for thyroid nodular disease concomitant with hyperthyroxinemia. Methods According to different clinical manifestations,traditional preoperative medical preparation, impulse preoperative medical preparation and propranolol preoperative medical preparation were respectively made on 43 cases of thyroid nodular disease concomitant with hyperthyroxinemia and without eye proptosis. Preoperative medical preparation wasn't performed on 12 eases of hyperthyroxinemia without apparent hypermetabolism and basal metabolic rate less than 20%. Hospitalization time and incidence of intraoperative and postoperative complication were observed and recorded. Results Intraoperative and postoperative thyroid crisis or serious complications didn't occur in all 43 cases of thyroid nodular disease concomitant with hyperthyroxinemia and without eye proptosis. Hospitalization time differs for patients with different preoperative preparation. Conclusion It was feasible to make individualized preoperative preparation for thyroid nodular disease concomitant with hyperthyroxinemia.

Objective:To investigate the knowledge, attitudes and practice of child sexual abuse (CSA) prevention education in parents of elementary school pupils.Method:Two hundred and seventy one parents were surveyed by anonymous self-administered questionnaire in five classes of grade 1 to grade 5 of a elementary school. The questions about awareness of CSA and communications on how to prevent CSA with their children were asked.Result:Among this sample, 93.6% of parents approved of school CSA prevention education. Overall, about half parents lack of knowledge of CSA prevention, the communications with their children were also not enough. Only 1.8% of parents had provided books or audiovisuals about CSA prevention for their children. The scores of knowledge, attitudes and practice of CSA prevention education of parents whose parents addressed this in their childhood were significantly higher than those of parents who had no this experience.Conclusions:The research of CSA prevention education in elementary school should be paid more attention, to produce related books and audiovisuals as soon as possible, to increase parents' awareness of CSA prevention.

Objective To explore the role of integrin in CCL18 for promoting breast cancer SK-3 rd cells invasion and migration process to illuminate the molecular mechanism of CCL 18 for promoting breast cancer SK-3 rd cells invasion and migration process . Methods The flow cytometry was adopted to detect CCL18-induced integrin aggregation ;Western blot was used to detect the focal adhesion kinase(FAK ) activation ,the infiltrating migrationin experiment was adopted to determine the invasion and migration of SK-3rd cells and the siRNAs transfection was used to detect the expression of silence integrin β1 .Results CCL18 promoted the in-tegrinβ1 aggregation in breast cancer SK-3rd cell surface and further promoted the integrin-mediated phosphorylated activation of FAK .Under the reaction of CCL18 ,the cells number of SK-3rd cellular invasion and migration was increased by ten times (P<0 .01) ,which was obviously decreased by siRNA silenced integrin β1 .Conclusion CCL18 promotes breast cancer invasion and mi-gration via integrin aggregation .