Adult guinea pigs with normal Preyer's reflexs were used in this experiment and were divided into three groups according to the intensity of noise and exposure time. Auditory brainstem evoked response was recorded before and after exposure to noise. Clicks were presented monaurally at a rate of high (50 Hz) as well as low (10 Hz). The superior olive and inferior colliculus were observed under the electron microscope after the guinea pigs were exposured to 110dB noise for 30 min. The average shift of wave Ⅳ amplitude-intensity function curve was more than that of wave I after exposure to intensive noise. The difference value of wave Ⅴ latency from low (10 Hz) to high (50 Hz) stimulus click rate decreased and was negatively co related with the exposure time. Ⅰ-Ⅴ, Ⅲ -Ⅴ and Ⅳ-Ⅴ interpeak latencies were shorter after exposure to noise. The mitochondria and neurilemma swelling were found in the superior olive and inferior colliculus after exposure. Some mechanisms of the changes of auditory center were discussed in this paper.

The head of guinea pig was irradiated with 60Co?-ray and a single dose of irradiation was 90 Gy and 70 Gy in two groups, respectively.Two h after irradiation, the threshold shifts were 12.5 dB and 9 dB, and the auditory cortical evoked response (ACER) maximum post-irradiation amplitude exceeded the maximum prc-irradiation value by 20% and 37%.Six h later, the threshold shift increased to 37 dB and the maximum amplitude decreased to the level of pre-irradiation in the 70 Gy group.Right auditory bulla area was irradiated with a total dose of 45 Gy in a course of six fractions in two weeks.Fourteen d after irradiation, the threshold shift of auditory brainstem evoked response(ABR)was 11.8 dB.Hjstolo-gical observation using scanning electron microscope showed that only outer hair cells in the basal coil of the cochlea were destroyed 6 h after irradliation in the 90 Gy group.But outer hair cells in all coils of the cochlea were found to be destroyed 16 h after irradiation.In the 45 Gy group, 14 d after irradiation, outer hair cells in all coils of the cochlea were damaged and no inner hair cells were injured severely.

Objective:To study the clinical characteristics of malignant lymphomas of the head and neck.Methods:The clinical data of 139 patients with malignant lymphomas of the head and neck were retrogradely analyzed,and the long term follow up was carried out.Results:Among all the cases there were 128 non Hodgkin's lymphoma(NHL) and 11 Hodgkin's disease(HD). The average ages of patients with HD and NHL were 41.8 and 50.0 years old respectively.There were lymphadenopathy in all patients with HD and 72.7% of those with NHL. Extranodal involvement occurred in 27.3% HD patients and 75.8% NHL patients; general symptoms appeared in 45.5% HD patients and 37.5% NHL patients. Most patients received chemotherapy of CHOP and improved CHOP scheme.The average follow up time was 23.4 months. Twelve patients died and the 3 year survival rate was 55.6%.Conclusion:In our group, the patients present an older age and NHL is more common than HD.The cases with cervical lymphadenopathy are easy to be diagnosed,and the clinical stage classification is useful for the prognosis.

A ZnO nanofibers carrier on the Pt electrode via electrospinning technology was prepared and then the Keggin phosphotungstic acid was deposited on the ZnO/Pt electrode using electrodeposition technology. The scanning electron microscopy showed that the zinc oxide fibers presented net structure and the diameter of its fiber was about 300 nanometers. The fourier transform infrared spectroscopy confirmed that the phosphotungstic acid adhered to the fiber surface. The electrochemical property of the modified electrode showed that it exhibited rapid response, excellent sensitivity and good stability, achieving rapid and accurate determination. The electrode responded linearly to ascorbic acid ( AA) in a concentration range from 8. 8 × 10-7 to 3. 3 × 10-4 mol/L with a low detection limit of 2. 5 × 10-7 mol/L. The method is promising for the development of AA detecting instrument.

In order to definitude the influence caused by the different sampling in voice assessment.Method:We comparing the results acquired by total section and subsection sampling.Result:The results acquired by subsection tended to normal more than those acquired by total section. Conclusion:Subsection sampling voice assessment might conceal the drgree of the disease state of patients

In order to study the effect of chronic noise exposure on auditory center, seven guinea pigs were .exposured to 105 dB A noise 8h daily for 45 d. Then the animals were killed by decapitation. The superior olive and inferior colliculus were taken immediately to make the samples of electron microscope. The mitochondria swelling, membrane rupture, cristae breaking, ballooning degeneration were found. The rough surfaced endoplasmic reticulum was swollen and expanded. The lysosome and secondary lysosome were increased. The cell interstitial edema and synaptic besicle decrease were observed. The nuclear chromatid was decreased and nucleolus was kept to the side. The laminae of neurilemma of nerve fibers were dissociated, swollen and broken. The results indicate that the ultrastructure, neural cells and fibers in the auditory center are damaged by chronic noise exposure and most of these damages are irreversible.

The changes of hair cells after acute radiation injury to cochlea were studied with scanning electron microscope in guinea pigs. Outer hair cell cilia were disordered, fused, and lost in the early stage after 40Gy ?-ray irradiation of the bullae of guinea pigs. From 15 to 30d after radiation, reconstruction of cilia besides early changes, and ball shape materials on the side of inner hair cells were found. The possible mechanism of these changes is also discussed

The effects of different doses of r-ray on cochlea are reported in this paper. Significant hearing loss and severe cochlea hair cells injury were found while radiation dose was more than 80 Gy. With 40 Gy to 60 Gy, slight hearing loss, but cochlea hair cells and support cells impairment were observed. With 20 Gy, no hearing loss and no hair cell damage were found. The results indicated that the damage increases with a dose of radiation and there is a delay effect of radiation on cochles.

OBJECTIVE To explore the changes in gene expression in patients with allergic rhinitis(AR). METHODS 14500 gene DNA microarray(Affymetrix) was used to examine the gene expression in 6 AR nasal mucosal samples and 6 normal nasal mucosal samples.The differentially expressed genes were identified and subjected to real time RT-PCR analysis. RESULTS Compared with normal sinus tissues,161 genes were found to be differentially expressed in AR samples.Forty-seven genes were upregulated,114 genes were downregulated.The differentially expressed genes mostly involved in immune transcription regulatory molecules,signal transduction.Real-time RT-PCR results of CCL20,GPK4 were consistent with that of gene chip analysis.CONCLUSION Microarray expression profile of AR samples is differential.GPK4 and CCL20 may play an important role in cell signal transduction in mechanisms of AR.

Objective To study the characteristics of cognitive function changes in patients with cerebellar infarction, and to clarify the association between cerebellar infarction and cognitive function. Methods A total of 106 patients with cerebellar infarction, admitted to our hospital from June 2017 to December 2018, and 55 healthy subjects recruited at the same time were chosen. National Institute of Health Stroke Scale (NIHSS) was used to evaluate neurological deficits, and modified International Cooperative Ataxia Rating Scale (ICARS) was used to evaluate balance function. The cognitive function differences of the two groups were compared by Mini-mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA). Patients with cerebellar infarction were divided into different subgroups according to different courses of disease, different infarction sites and different cerebellar hemispheres to further compare the cognitive functions of the subjects. Results (1) In terms of cognitive function evaluation, the scores of MoCA and Rey auditory word learning test (RAVLT) in the cerebellar infarction group were significantly lower than those in the control group, and the time consumption of Trail Making Test (TMT)-B was significantly longer than that in the control group (P<0.05). (2) MoCA and RAVLT scores in the acute cerebellar infarction group were significantly lower than those in the non-acute cerebellar infarction group (P<0.05). (3) There were significant differences in ICARS scores, MoCA scores, RAVLT scores and TMT-B time consumption between the anterior cerebellar infarction group and the posterior cerebellar infarction group (P<0.05). (4) The time consumption of TMT-B of right cerebellar infarction patients was significantly longer ([258.85 ±55.96] s) than that in left cerebellar infarction patients ([198.22 ±40.43] s, P<0.05). Conclusions There are cognitive dysfunctions, especially memory and executive function, in patients with cerebellar infarction, which is correlated with the disease period and location of cerebellar infarction, but not with infarct volume. Cognitive dysfunction is more obvious in patients with acute cerebellar infarction, posterior cerebellar infarction and right cerebellar infarction than those in patients with non-acute cerebellar infarction, anterior cerebellar infarction and left cerebellar infarction.