Objective To observe the protective effect of Qiangxin oral liquid on experimental left ventricular hypertrophy induced by isoproterenol and ultrastructure in rats.MethodsMyocardial hypertrophy model of rats were established by injection of isoproterenol (5 mg/kg/d) for 7 days. Thirty rats were divided into the control group, model group and treatment group (treated with Qiangxin oral liquid from second day after myocardial hypertrophy model made and continued for 12 weeks). Cardiac structure and function were detected in all groups by ultrasonography on 2nd, 6th and 12th week after model made respectively. After 12 weeks, the left ventricular weight/body weight index (LVW/BW) of all animals was tested and myocardial ultrastructure was examined.ResultsCompared with the model group, the interventricular septal end systolic thickness, left ventricular posterior wall thickness, left ventricular diameter, and left ventricular mass index of the treatment group decreased significantly ( P<0.05～0.01), while the damage of myocardial ultrastructrue lightened.ConclusionQiangxin oral liquid can improve the experimental myocardial hypertrophy of rats induced by isoproterenol.

Objective To investigate the variation law of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) values in patients with cerebral infarction, and to explore the relationship between the changes and the prognosis of cerebral infarct patients. Methods Sixteen patients with cerebral infarction were recruited and divided into 2 groups:good recovery and poor rehabilitation. ADC and FA values were calculated in infarct areas and control areas which were the regions with symmetrical position and the same area as infarct areas. The difference of ADC and FA values in patients at acute and earlier chronic stage between the two areas were analyzed. Results ①At acute stage, ADC values in infarct areas were lower than those in control areas (P<0.05). At early chronic stage, there was no significant difference of ADC values between infarct areas and control areas (P>0.05), moreover ADC values were higher than that at acute phase (P<0.05). ②FA values in infarct areas were lower than those in control areas at both acute and early chronic stage (P<0.05). At early chronic stage, FA values were lower than those at acute stage (P<0.05). ③There was no significant difference of ADC and FA values at both acute and early chronic stage between good recovery group compared with poor rehabilitation group (P>0.05). Conclusion There are certainly rules in changes of ADC and FA values in patients with cerebral infarction at acute and earlier chronic stage.

OBJECTIVETo study the expression of CD70 and the methylation level of CD70 promoter in immuno-related pancytopenia（IRP）patients, and explore the role of CD70 in the pathogenesis of IRP.

METHODSThirty- five IRP patients and fifteen healthy donors were enrolled in this study. Peripheral blood mononuclear cells were isolated from venous blood by density gradient centrifugation, and CD4(+) T cells were isolated by immunomagnetic beads. The mRNA level and the percentage of CD70 of CD4(+) T cells were measured by real- time quantitative polymerase chain reaction（RT- PCR）and flow cytometry respectively. Methylation- Specific PCR（MSP）was performed to determine the methylation level of CD70 promoter.

RESULTSThe percentage of CD70 expression on CD4(+) T cells of untreated IRP patients［（7.46±1.51）%］was significantly higher than that of recovered ones［（5.95±1.34）%］and normal controls［（1.83 ± 0.60）%］, and that of recovered IRP patients was significantly higher than of normal controls（P<0.05）. The relative expressions of CD70 mRNA in CD4(+) T cells were 2.314（0.200-6.084）, 1.021（0.135-3.434）, 0.353（0.008-2.258）in three groups respectively. The differences among untreated IRP patients, recovered IRP patients and normal controls were significant（P<0.05）. The CD70 promoter methylation level in CD4(+) T cells of all IRP patients was significantly lower than that of normal controls （P<0.05）. The expression of CD70 positively correlated to the ratio of CD5(+) B cells（r=0.533, P<0.01）.

CONCLUSIONThe overexpression of CD70 may lead to immunologic disarrangement of patients, which may play important role in the pathogenesis of IRP.

B-Lymphocytes ; CD27 Ligand ; CD4-Positive T-Lymphocytes ; Flow Cytometry ; Humans ; Pancytopenia ; Promoter Regions, Genetic ; RNA, Messenger ; Real-Time Polymerase Chain Reaction