Objective To explore the predictive value of soluble growth STimulation expressed gene 2(sST2) on major adverse cardiovascular events(MACE) of acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI).Methods The study included 148 patients with first episode of AMI admitted from January 2015 to May 2016 in the heart center of the First Hospital of Lanzhou University.Serum sST2 level before PCI was tested and all patients were followed up clinically for 6 months after PCI.Results 1.MACEs were found in 23 patients during follow up.The sST2 leveles were significantly higher in patients with MACEs than the non-MACE group [(44.50 ±5.32) ng/ml vs.(23.59±1.15) ng/ml, P=0.001].Pearson correlation analysis showed that serum sST2 were positively correlated with MACE and type Ⅲ procollagen amine terminal peptide (PⅢNP) but was not correlated with NT-proBNP.2.Serum sST2 found to be correlated with the body mass index, blood pressure, triglycerides, aspartate aminotransferase, left ventricular end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF).3.The area under the ROC curve of sST2 to predict the occurrence of MACE after PCI was 0.787 which was higher than that of NT-proBNP.The area under curve of sST2 combined with NT-proBNP was 0.820.4.The survival rate of patients with serum sST2 level ≤29 ng/ml was higher than patients with sST2>29 ng/ml in 6 months after PCI.Conclusions sST2 is affected by a variety of factors.sST2 combined with NT-proBNP can improve the predictive value of MACE after PCI, and higher the level of sST2, higher the mortality rate in 6 months after PCI.

Aim To explore the correlation between atherogenic index of plasma(AIP),triglyceride-glucose(TyG)index and their severity in patients with chronic coronary syndrome(CCS).Methods A total of 298 patients diagnosed with CCS by coronary angiography were retrospectively selected from Cardiology Department of the First Hospital of Lanzhou University,from May 2017 to May 2023.Clinical indexes were collected and Gensini scores were calculated based on the results of the coronary angiography,clinical data of different Gensini integral groups was compared.Linear regression was used to analyze factors that influence the elevation of Gensini scores,and receiver operator characteristic(ROC)curve was used to determine the predictive value of AIP and TyG index for the severity of coronary artery lesions in CCS.Results The research sample consisted mostly of males(77.9％)with a mean age of(61.9±8.0)years.The adjusted AIP(aAIP)and TyG index of high Gensini score group were higher than those of low Gensini score group and medium Gensini score group,and the differences were significant.Linear regression analysis revealed that total cholesterol(TC),high density lipoprotein cholesterol(HDLC),aAIP and TyG index were the factors influencing the ele-vated Gensini scores(all P＜0.05).Correlation analysis revealed that aAIP and TyG index were negatively correlated with left ventricular ejection fraction(LVEF),positively correlated with Gensini scores,and positively correlated with stent implantation and the number of stent implantation(all P＜0.05).The ROC curve results indicated that when the aAIP threshold was 1.924,the area under the ROC curve(AUC)for predicting Gensini score ≥41 points was 0.583(95％CI 0.525～0.640),with a sensitivity of 92.62％,a specificity of 25.50％,and Yoden index of 0.181.When the TyG in-dex threshold was 8.748,the AUC for predicting Gensini score ≥41 points was 0.768(95％CI 0.716～0.815),with a sensitivity of 77.18％,specificity of 67.11％,and Yoden index of 0.443.Conclusion The aAIP and TyG index were positively correlated with the severity of coronary artery disease in CCS patients.Both elevated levels can predict the severity of coronary lesions in CCS patients,but TyG index showed superior predictive ability compared with aAIP.

Objective To observe the effects of Bushen Zhuyun Prescription on regulating mitochondrial function and endometrial angiogenesis;To explore its mechanism of improving endometrial receptivity.Methods The mouse model of controlled ovarian hyperstimulation(COH)was established,and the mice were randomly divided into normal group,model group,and Bushen group,with 20 mice in each group.Bushen group received Bushen Zhuyun Prescription for gavage for 11 d,and the normal group and model group received normal saline for gavage.The number of embryo implantation was counted,the endometrial morphology was observed by HE staining,α-smooth muscle actin(α-SMA)expression was observed by immunofluorescence staining.Human endometrial microvascular endothelial cells(HEMECs)were cultured in vitro,they were divided into control group,VEGFA group,Bushen group and VEGFA + Bushen group,and were intervened with VEGFA and/or Bushen Zhuyun Prescription medicated serum.The activities of mitochondrial respiratory chain complexes Ⅰ-Ⅳ,the content of ATP,the expression of PCNA and Caspase-3 were detected.Results Animal experiment showed that,compared with the normal group,the number of embryo implantation in model group significantly decreased(P<0.05),α-SMA protein expression in endometrial tissue significantly decreased(P<0.05);compared with the model group,the number of embryo implantation in Bushen group significantly increased(P<0.05),α-SMA protein expression in endometrial tissue significantly increased(P<0.05).Cell experiment showed that,Bushen Zhuyun Prescription medicated serum could increase the activity of mitochondrial respiratory chain complexes Ⅰ-Ⅳ and ATP content in HEMECs,promote PCNA protein expression,and inhibit Caspase-3 protein expression(P<0.05,P<0.01).Conclusion Bushen Zhuyun Prescription can promote endometrial angiogenesis through improving mitochondrial function.