Objective To analyze the effect of duloxetine on S100B and signal pathway ERK1/2-NF-κB expression in hippocampus in depression rat.Methods Chronic unpredictable mild stimulation was used to establish depressive model rats (n=50).They were randomly divided into no-intervention group (n=10),different treatment time of duloxetine group (C,D,E,F group,10 rats in each group)and then 10 normal rats were selected as control group.Behavior tests including open-field test and the saccharine preference test were used to test the behavioral change of rats after 28 days intragastric administration.Western blot was used to detect S100B,t-ERK1/2,pERK 1/2,t-NF-κB and pNF-κB expression in hippocampus.Results In open-field test,the crossing score,rearing score and latency of the rats in E,F group were (69.68± 14.61) and (70.66± 11.53) score,(20.94 ± 10.92) and (20.32±8.85) score,(1.1±0.4)s and(1.0±0.4) s respectively,and showed no significant difference with those of control group ((71.19±12.08) score,(20.42±8.76) score,(1.0±0.3)s) after 28 d intragastric administration (P＞0.05),while the level score,vertical score were significantly higher than those in depressive model (P＜ 0.05).In the saccharine preference test,the rats in E,F and control group exhibited increased saccharin preference compared with depressive model rats (P＜0.05).The rats in E,F and control group exhibited increased S100B,pERK1/2 and pNF-κB expression in hippocampus compared with depressive model rats (P＜0.05).Conclusion Duloxetine improves the behavioral ability of depression rat and exerts effect after 2 weeks.The ERK1/2-NF-κB signal pathway in hippocampus may participate in this mechanism.

70 patients including 60 cases under acupuncture anesthesia and 10 cases under drug anesthesia were observed. Before and 20 minutes after acupuncture the blood samples were taken from the patient ear lobes respectively, and in some patients taken once again 24 hrs after acupuncture. The electroacupuncture point Hegu or Zusanli was mainly adopted. As the method for detection of cell-mediated immunity(CMI) in vitro the improved microtechnique of whole blood for E-rosette (active and nonactive) and lymphocyte transformation tests was used. In performance of active rosetting the total leucocyte count and the differential lymphocyte count were done for calculation of absolute number of active rosette forming cells (RFC). The mean value of increase of active RFC was 12.7?1.43, the decrease was 6.8?1.77 after acupuncture. The increment of the absolute number of active RFC was 175?63.59. However no marked effect on the drug anesthesia group was found. In the lymphocyte transformation assay the increase was 12.7?1.49, the decrease was 7.0?2.19, and the enhancement effect still exhibited 24 hrs after acupuncture. In these tests an increase was mostly found in those with a lower or a usual CMI level; a decrease often found in those with a higher CMI level prior to acupuncture. The increase or decrease level in the results of three kinds of test (active, nonactive RFC and lymphocyte transformation) was similar, the increase range was 12～13%, the decrease range 6～7%. As the former compared with the latter, the promotion was prominent by all means.

As the key to regeneration of oral and maxillofacial tissues such as bone, dental pulp and skin, vascularization has always been the focus of tissue engineering. With the development of three-dimensional (3D) printing in tissue engineering, there are increasing ways to construct vascular structures. However, to achieve the objective of highly simulating vascular structure in morphology and function and promote tissue repair, it is still a major difficulty for 3D bioprinting to construct highly precise and biologically functional simulated vascular structures. This paper summarizes new progress of 3D printing vascular structure, expounds frontier biological manufacturing technologies of vascular and vascularized structure such as suspension printing, coaxial printing, 4D printing, and so on, analyzes its advantages and disadvantages, and discusses its development prospect, in order to provide reference for the application of 3D printing blood vessels in regeneration and repair of oral and maxillofacial tissues.

Objective To investigate the expressions of the β-catenin/transcription factor 4(TCF4)signaling pathway-related proteins in colon cancer tissues and their relationship with postoperative recurrence and metastasis of colon cancer.Methods A total of 86 patients with colon cancer admitted to Zhumadian Central Hospital from January 2020 to January 2022 were selected as the research subjects,all of whom underwent surgical resection of the colon.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression levels of Wnt-1,β-catenin and TCF4 mRNA in cancer tissues and adjacent tissues of patients,and immunohistochemistry was used to detect the protein expression levels of Wnt-1,β-catenin and TCF4.The Cox proportional hazards regression model was used to analyze the risk factors of postoperative recurrence and metastasis of colon cancer.Results The relative mRNA expression levels and protein expression positive rates of Wnt-1,β-catenin and TCF4 in cancer tissues were significantly higher than those in adjacent tissues of colon cancer patients(P＜0.05).The expression positive rates of Wnt-1,β-catenin and TCF4 protein were correlated with the degree of tumor invasion,tumor stage and lymph node metastasis(P＜0.05),but not correlated with age,sex,tumor size and differentiation degree(P＞0.05).Cox regression analysis showed that lymph node metastasis,and positive protein expressions of Wnt-1,β-catenin and TCF4 were risk factors for postoperative recurrence and metastasis of colon cancer(P＜0.05).Conclusion The mRNA expression levels and protein expression positive rates of Wnt-1,β-catenin and TCF4 are higher in colon cancer tissues.The protein expression positive rates of Wnt-1,β-catenin and TCF4 are significantly correlated with the degree of tumor invasion,tumor stage and lymph node metastasis,and are risk factors for postoperative recurrence and metastasis of colon cancer.

Traumatic rib fractures are the most common injury in thoracic trauma. Previously，the patients with traumatic rib fractures were mostly treated non-surgically，of which 50%，especially those combined with flail chest presented chronic pain or chest wall deformities and over 30% had long-term disabilities，being unable to retain a full-time job. In the past two decades，thanks to the development of internal fixation material technology，the surgical treatment of rib fractures has achieved good outcomes. However，there are still some problems in clinical treatment，including inconsistency in surgical treatment and quality control in medical services. The current consensuses on the management of regional traumatic rib fractures published at home and abroad mainly focus on the guidance of the overall treatment decisions and plans，and relevant clinical guidelines abroad lacks progress in surgical treatment of rib fractures in recent years. Therefore，the Chinese Society of Traumatology affiliated to Chinese Medical Association and Chinese College of Trauma Surgeons affiliated to Chinese Medical Doctor Association，in conjunction with national multidisciplinary experts，formulate the Chinese Consensus for Surgical Treatment of Traumatic Rib Fractures（2021）following the principle of evidence-based medicine，scientific nature and practicality. This expert consensus puts forward some clear，applicable，and graded recommendations from aspects of preoperative imaging evaluation，surgical indications，timing of surgery，surgical methods，rib fracture sites for surgical fixation，internal fixation methods and material selections，treatment of combined injuries in rib fractures，in order to provide references for surgical treatment of traumatic rib fractures.

Here, we report a previously unrecognized syndromic neurodevelopmental disorder associated with biallelic loss-of-function variants in the RBM42 gene. The patient is a 2-year-old female with severe central nervous system (CNS) abnormalities, hypotonia, hearing loss, congenital heart defects, and dysmorphic facial features. Familial whole-exome sequencing (WES) reveals that the patient has two compound heterozygous variants, c.304C>T (p.R102*) and c.1312G>A (p.A438T), in the RBM42 gene which encodes an integral component of splicing complex in the RNA-binding motif protein family. The p.A438T variant is in the RRM domain which impairs RBM42 protein stability in vivo. Additionally, p.A438T disrupts the interaction of RBM42 with hnRNP K, which is the causative gene for Au-Kline syndrome with overlapping disease characteristics seen in the index patient. The human R102* or A438T mutant protein failed to fully rescue the growth defects of RBM42 ortholog knockout ΔFgRbp1 in Fusarium while it was rescued by the wild-type (WT) human RBM42. A mouse model carrying Rbm42 compound heterozygous variants, c.280C>T (p.Q94*) and c.1306_1308delinsACA (p.A436T), demonstrated gross fetal developmental defects and most of the double mutant animals died by E13.5. RNA-seq data confirmed that Rbm42 was involved in neurological and myocardial functions with an essential role in alternative splicing (AS). Overall, we present clinical, genetic, and functional data to demonstrate that defects in RBM42 constitute the underlying etiology of a new neurodevelopmental disease which links the dysregulation of global AS to abnormal embryonic development.
Female ; Animals ; Mice ; Humans ; Child, Preschool ; Intellectual Disability/genetics* ; Heart Defects, Congenital/genetics* ; Facies ; Cleft Palate ; Muscle Hypotonia