AIM: To investigate the effect of different types of flavonoids, galangin (flavonol), hesperetin (flavonone) and hydroxysafflor yellow A (HYSA, chalcone), on cardiomyocytic injury induced by H2O2, and explore the possible signal pathways involved. METHODS: The cytotoxicity of different flavonoids was determined by MTT assay. Apoptosis rate was determined by flow cytometry (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Immunohistochemistry was used for detection of Bcl-2, Bax and Caspase-3 protein. RESULTS: It showed that each flavonoid did not have noticeable cytotoxicity at a concentration of 5 ?mol/L by MTT assay. Flavonoids at concentrations of 5, 15 and 30 ?mol/L significantly increased cell viability compared to model group induced by H2O2 (100 ?mol/L). Flavonoids also increased apoptosis rate and neorobiosis rate determined by FCM compared to model group. Galangin and hesperetin significantly decreased the apoptotic rate determined by TUNEL and the expression of Caspase-3 and increased the ratio of Bcl-2/Bax (P

ObjectivesTo discuss the value of the three kinds of surgical management to treat high-grade cervical intraepithelial neoplasia. MethodsThe study was conducted on 136 patients who underwent LEEP、CKC 、hysterectomy ,to compare the change of the postoperative biopsy result,the rate of cure and pregnancy. ResultAmong the postoperative biopsy result,66. 4% was consitent with pretreating histopathology,but 3.6% was upgrade,30.0% was downgrade; there were no statistical difference in the rate of cure of the three kinds of surgical management and in the rate of pregnancy of the two kinds of conization. ConclusionThe three kinds of surgical management to treat high-grade cervical intraepithelial neoplasia were effective,while LEEP was the best way.

Objective To analyze the clinical management of women with atypital squamous cells(ASC).Methods During Sep 2003 to Sep 2008,a totle of 956 women in our department were diagnosed with ASC by cytological test,they were further biopsied under the colposcope.The result of TCT,colposcopic test,biopsy and the highrisk factor were recorded and analysed;the based the biopsy were analyzed by the X~2-test;the high-risk factors and the odds Ratio(OR) were counted by SPSS10.0.Results Among the women with ASC,the rate of cervical intraepithelial neoplasia(CIN) diagnosed by the biopsy under the colposcope was 50.7% ,the rate of the high-grade lesion over CINII Was 13.3% ;if the colposcopic test were low-grade lesion,the rate of the cases which biopsys were the highgrade lesion over CINII,was 8.3% ;the high-risk factors which caused the cervical high-grade lesion were the number of sex-partners over 3,the age less than 20 yearold when the sex were made,the cervical erosion,the result of TCT with ASC-H.Conclusion Among the women with ASC,the biopsy shows multiformity;the rate of CIN and the highgrade lesion is also high;if TCT is ASC,the colposcopie test is advised;if the woman who has the high-risk factors,biopsy is advised.

Anthracyclines, which include doxorubicin, epirubicin, daunorubicin, and aclarubicin, are widely used chemotherapeu-tic agents for treating hematologic and solid tumors, such as acute leukemia, lymphoma, breast cancer, gastric cancer, soft tissue sarco-mas, and ovarian cancer. Anthracyclines can be combined with other chemotherapeutics and molecular targeted drugs for cancer treat-ment. The combination of anthracyclines with other chemotherapeutic drugs is usually the standard of first-line treatment. Anthracy-clines are efficacious and potent agents with broad antitumor effects. However, these drugs may cause adverse reactions, such as hair loss, myelotoxicity, and cardiotoxicity. Hematopoietic stimulating factors, such as granulocyte colony-stimulating factor, erythropoietin, and thrombopoietin, can be used to control myelotoxicity. However, cardiotoxicity is the most serious anthracycline side effect. Clinical study results and practical observations indicate that the anthracycline cardiotoxicity is usually progressive and irreversible, especially after the first use of the drug, which may particularly cause heart damage. Therefore, the early detection and prevention of anthracy-cline-induced cardiotoxicity are important and have already gained considerable attention in clinical applications. Relevant experts from the China Society of Clinical Oncology and Hematology Branch of the Chinese Medical Association prepared the guidelines for the pre-vention and cure of anthracycline-induced cardiotoxicity in 2013. The authors reviewed the effective drugs currently used to prevent and cure anthracycline cardiotoxicity.

Objective To retrospectively analyze the effect of interventional embolization for hepatocelluar carcinoma (HCC) associated with arteriovenous shunting (AVS), and to discuss the factors influencing the therapeutic results. Methods The clinical data of 62 cases with HCC associated with AVS , who were treated with interventional chemoembolization , were retrospectively analyzed. Based on the type and extent of AVS identified by angiographic manifestations, appropriate obstruction of the shunt and Lipiodol chemoembolization of HCC were conducted. The curative effect of the shunt embolization was assessed by DSA at one or two months after the treatment. The relevant factors influencing the prognosis of embolization were analyzed by using univariate and multivariate Cox regression analysis methods. Results Of the 62 patients, arterioportal shunting (APS) was detected in 44, hepatic arterio-venous shunting (HAVS) in 11, APS together with HAVS in 4, and hepatic artery-pulmonary artery shunting (HAPAS) in 3. Re-examination with DSA was carried out in 53 patients at 1 - 2 months after the treatment , which showed that the shunting disappeared in 18 cases, obvious reduction of the shunt flow was seen in 19 cases, the lesion remained stable in 9 cases and the disease became worse in 7 cases. Univariate analysis indicated that the kind of embolic material and the presence of tumor thrombus could affect the obstructive result of the shunt , while multivariate Cox regression analysis showed that portal tumoral thrombus was an independent risk factor. The embolization effect of polyvinyl alcohol (PVA) particles and Lipiodol-ethanol mixture, used as the embolic agents, was better than that of gelatin sponge particles. Conclusion To ensure a successful interventional chemoembolization for HCC combined with AVS the procedure should be individualized according to the type and extent of the arteriovenous shunting. The type of embolic materials used for embolization can affect the results to a certain degree.

Objective To investigate the influence of acquisition time of C-arm cone-beam CT on image quality and radiation dose of cerebral angiography.Methods C-arm cone-beam CT of cerebral angiography was performed on the male anthropomorphic head phantom,with DynaCT imaging mode and the acquisition time of 5 s,8 s and 20 s were used.Scanning was performed with each acquisition time for three times,and VR,MIP and MPR images were reconstructed.The attenuation values and their standard deviations of intracranial segment of the internal carotid artery (ICA),middle cerebral artery(MCA),anterior cerebral artery(ACA) and uniformed brain tissues were measured to calculate the image noise,signal to noise ratio (SNR) and contrast to noise ratio (CNR).We used the image noise,SNR and CNR as the objective standard to evaluate the image quality,and One-way ANOVA analysis of variance was used to assess the difference among them.A scale with scores 1 to 5 was used to rate the quality of the reconstructed image of ICA,MCA,ACA as a subjective evaluation,the difference among the evaluation scores were analyzed using Kruskal-wallis.We recorded the dose area product (DAP) of each acquisition time and the effective dose(ED) was calculated to assess the radiation dose,the difference among them were analyzed using One-way ANOVA analysis of variance.Results In C-arm cone beam CT 20 s imaging,the SNR of intracranial segment of the ICA,M CA,ACA were 22.29± 1.41,29.36 ±0.11 and 23.13 ±2.10 respectively,in 5 s imaging13.83 ±0.61,14.65 ±0.16 and 12.79±0.19 respectively,in 8 s imaging 14.92±0.96,18.97 ± 1.24 and 16.65 ±0.46 respectively,all the results showed a significant difference (F valued 58.19,327.29,52.74 respectively,all P valued＜0.01),the CNR of 20 s imaging were higher than that of 5 s and 8 s imaging,the Noise of ICA,MCA,ACA and the uniformed brain tissues of 20 s imaging were lower than that of 5 s and 8 s imaging,all the results showed significant difference (all P valued＜0.01).The subjective evaluation scores of VR imaging of 5 s,8 s and 20 s were 3.61 ±0.49,4.06±0.53,4.72±0.45 respectively,the scores of MIP imaging were 3.42±0.50,3.83±0.65 and 4.50±0.51 respectively,the scores of MPR imaging were 2.83±0.45,3.14±0.35 and 3.67±0.49 respectively,all the results showed significant difference (x2 valued 51.29,42.25 and 43.56 respectively,all P valued ＜0.01).The DAP values in C-arm cone beam CT 5 s,8 s and 20 s imaging were (9.11 ±0.18),(13.81±0.75)and(58.62±0.62)Gy· cm2,ED values were (0.91 ±0.02),(1.38±0.75)and(5.84± 0.11) mSv,all the results showed significant difference (F valued 3 720.30 and 3 654.85 respectively,all P valued＜0.01).Conclusion The image quality and radiation dose of C-arm cone-beam computed tomography are closely correlated with acquisition time.

The characteristics of ductal adenocarcinoma of colon under eieciror: microscopy were stout and dense microvill, with small roots buried in the top of cytoplasm. The ullrarnorphological changes of carcinomatous infiltration was similar to the picture observed in vitro cultivation of cancer cells.In 104 cases out of 159, there was an over expression of ras oncogenes P21, indicating that carcinoma of colon was related to activation of ras oncogenes. Examination with monoclonal antibodies yielded a positive rate of 83.4%- 95.7%, indicating that most of the carcinomas had colon related antigens. This phenomenon should play an important role in raising the diagnostic accuracy, and it also should serve as an indicator of effective treatment. In 62 cases of mucoid adenocarcinoma and 30 cases of signet ring cell carcinoma, there was a marked reduction of mueoitin sulfate with an increase in sialic acid mucus. The letter was a glycoprotein containing hydroxyl mucus, and it facilitated the separation of cancer cells to infiltrate and metastasize.in 134 cases, AgNOR was quantified, and there were 13.05? 1.48 granules in each mucleas. There was more abundant AgNOR in undifferentiated cancer, while the number of AgNOR fluctuated between 10.56 to 11.83 in papillary adenocarcinoma, ductal adenocarcinoma, and mucoid adenocarcinoma. indicating the qunantity of AgNOR was related to degree of malignancy in colonic carcinoma.

Objective To study the influence of various doses and injection rates of contrast agent on CT perfusion in rabbits' liver with a deconvolution mathematical model. Methods Eight rabbits were enrolled in the experiment. Randomized block design was adopted. The treatment factor (contrast medium injection rate) was classified into 0.5, 1.0, 1.5 ml/s, while the subjects were divided into 3 blocks with contrast medium injection dose of 0.5, 1.0 and 1.5 ml/kg. The data obtained at CT perfusion imaging were then transferred to the workstation. Absolute values of 7 perfusion parameters (hepatic arterior fraction, blood flow, blood volume, permeability surface, mean transmit time, hepatic artery perfusion and portal vein perfusion) were measured with perfusion software (Perfusion 3). Results The dose of contrast medium had significant effect on peak enhancement of the aorta, the portal vein and liver tissue (P<0.05), whereas the injection rates had significant influence on the arrival time to peak enhancement of the aorta and the portal vein. However, the dose of contrast medium and injection rates had no significant effect on perfusion parameters (P>0.05). Conclusion CT perfusion imaging with a deconvolution mathematical model can quantify the hemodynamic functional status in liver with stable results. This technique does not need strict confinement to dose and injection rate of contrast medium, and has great potential value to be put into clinical use.

ObjectiveTo evaluate the technical feasibility of animal model of avascular necrosis of femoral head (ANFH)with transcatheter arterial embolization (TAE).MethodsTwenty experimental pigs were randomly divided into experimental group and control group (each n= 10).Experimental group:A 5F Cobra catheter was inserted into left femoral artery,and the feeding arteries of femoral head were superselectively inserted.The feeding arteries were embolismed through transcatheter arterial injecting the segments of silk measuring about 500μm.Control group:The arterial embolization was not performed,and the other treatment was identical to experimental group.The articulation of hip in all pigs underwent plain X-ray examination,CT and MR scanning 2 weeks and 4 weeks after treatment,respectively.Histological examination was made in 4 weeks to evaluate volume of bone trabecula (TBV) and percentage of bone lacuna (PBL) at unit area under microscope.The data were compared between the two groups.Results In experimental group,CT and MRI showed swolling in hip soft tissue and high T1 in hip joint cavity,while no obvious abnormalities were found in plain X-ray film 2 weeks after feeding arteries were embolized.Four weeks after feeding arteries embolization,plain X-ray film,CT and MR showed typical necrosis of femoral head in the experimental group,while no obvious abnormalities were found in control group.The histology examination revealed there were obvious karyopyknosis and anachromasis in the bone cells.The quantity of bone cells decreased obviously or disappeared.PBL increased and TBV decreased significantly compared with those of control group (P＜0.05).ConclusionThe animal model of ANFH in pigs can be induced by TAE.It can preferably mimic the pathological situation of ANFH.