[Objective]To observe the effects of borneol on the pharmackinetics of scutellarin after intranasal administration in rats.[Method]The different time of plasma scutellarin concentration was determined by the method of 125I label after iv injection or intranasal administration,intranasal administration of scutellarin(combined with 1% borneol)0.4 mg?kg-1 in SD rats.To calculate the pharmacokinetic parameters by 3P87 software.[Result]The time of maximum concentration(Tmax)and the maximum concentration(Cmax)at the class of intranasal administration is 25.0 min,0.55ug/ml,at the class of intranasal administration(combined with borneol)46.5 min,0.50ug/ml;the absolute bioavailability of intranasal administration is 53.70%,while the intranasal administration(combined withborneol)is 52.86%,there is no statistically significant differences compared with each other.[Conclusion]The borneol prolongs the peak time of plasma scutellarin concentration after intranasal administration in rats,but AUC is not influenced.

Objective compare the changes in condyle positions, face type lateral position and facial soft tissue before and after clinical correction of adult ClassⅡ Division Ⅰ malocclusion patients accompanied with the temporomandibular disorder. Method there were 7 adult Class Ⅱ Division Ⅰmalocclusion patients with accompanied with temporomandibular disorder, they accepted orthodontic extraction treatment routinely with MBT straight wire appliance technology, by which the occlusion was opened, the front teeth was received within correction, the occlusion was finely adjusted to canine fossa staggered relationship and the overbite cover was proper. Results The face type facial soft tissue profile was changed from raised face before treatment to straight face type and 1/3 of height under the face was increased and the temporomandibular disorder was made better. Conclusion the molars occlusion relationship of adult Class Ⅱ Division Ⅰ malocclusion patients accompanied with temporomandibular disorder was adjusted by opening the occlusion and adducting the upright front teeth , the condylar process was moved forward , the posterior teeth alveolar bone height was increased and the temporomandibular disorder was substantially improved.

Objective To compare the efficacy of supraclavicular approach and infraclavicular approach in ultrasound-guided brachial plexus block(BPB).Methods One hundred and twenty patients,ASA Ⅰ-Ⅲ,aged 18-80 yr,scheduled for upper extremity surgeries of both gender,were randomized into two groups(n =60):supraclavicular group(group SCB)and infraclavicular group(group ICB).The anesthetic mixture consisted of ropivacaine 0.375％ and lidocaine 1％ in equal volumes with epinephrine 1∶200 000,the total dose was 0.5 ml/kg.The block performance time,duration of anesthesia and success of anesthesia(surgery was accomplished without supplementary block)were recorded.A blinded observer assessed pinprick sensory block in the seven distal nerve territories(axillary,radial,musculocutaneous,median,ulnar,medial antebrachial and medial brachial cutaneous nerves)every 5 min up to 30 min after injection.Success of nerve block,side effects and complications were recorded during and after operalion.Results Group ICB was superior in success rate of anesthesia and nerve block of ulnar,medial antebrachial and medial brachial cutaneous nerves,the rate of parasthesia was lower and the block performance time was longer compared with group SCB(P ＜ 0.05).No major complications occurred in both groups.Conclusion Under ultrasound guidance,infraclavicular BPB is superior to supraclavicular approach.

Promotion of outstanding people to head nurses is key to hospital nursing management.In view of the current shortcomings in the analysis of appraisal data and the interpretation of appraisal results,the analysis of means(ANOM)method is introduced to the process of hospital human resource management.The proposed method aims to solve the problem when the score differences between interviewers are great and the score differences between candidates are small.An example for head nurses' promotion appraisal in a hospital is offered to demonstrate that statistical tools can provide the decision support for hospital personnel selection.Finally,the application differences between ANOM and analysis of variance(ANOVA) in human resources appraisal are compared.

BACKGROUND:Radiation-induced brain injury has recently become an increasing area of research, in particular in animal experimental studies. Domestic and international researches show that there have been no uniform scanning parameters used for examination of animal models of radiation-induced brain injury by magnetic resonance imaging. In this study, we performed magnetic resonance imaging in rabbits to determine related sequence parameters. OBJECTIVE:To establish the New Zealand rabbit models of radiation-induced brain injury, and obtain the brain magnetic resonance images of rabbits using LOOP7 coil, so as to provide experimental evidence for diagnosis of radiation-induced brain injury by magenetic resonance imaging. METHODS:Each of T2-weighted imaging, diffusion tensor imaging, magnetic resonance spectroscopy and magnetic susceptibility-weighted imaging were performed several times through the use of LOOP7 coil, to determine the optimal scanning parameters for each sequence. Rabbit models of radiation-induced brain injury were established and then their right hemispheres were irradiated using 6 MV X-rays at a single dose of 40, 80 and 120 Gy. The daily performance and dynamic magnetic resonance signs of rabbits were observed. The brain tissue was taken for pathological examination once abnormal magnetic resonance findings were observed or after 20 weeks of folow-up. RESULTS AND CONCLUSION:Only one rabbit model in the 40 Gy group had subdural hemorrhage. In the 80 Gy group, abnormal T2-weight imaging signals were observed in al rabbit models, which were pathologicaly confirmed as scattered degenerated neurons and infiltrated neutrophils. The abnormal signals that gradualy expanded over time were seen in rabbits from the 120 Gy group by magnetic resonance imaging and were pathologicaly confirmed as radiation-induced brain injury loci. The results confirm that establishing rat models of radiation-induced brain injury using radiation therapy system can better simulate the pathological process of radiation-induced brain injury; moreover, this model can be applied to receive routine magnetic resonance examination with LOOP7 coil.

Objective To investigate the effects of treadmill training at different intensities on neurological function and expression of kalirin-7 in rats after cerebral ischemia-reperfusion,and the possible mechanism.Methods Sixty Wistar male rats were randomized into a model group,a routine training group,an intensive training group and a sham-operation group (n =15 for each group).A middle cerebral artery occlusion/reperfusion (MCAO/R) model was established in all the rats of the first three groups.The model group and sham group did no motor training,while the utine training group did treadmill training once daily,and the intensive training group did treadmill training twice a day.On the 3rd,7th and 14th day,Zausinger scores was used to evaluate the neurological deficit;reverse Transcription PCR (RT-PCR) and Western blot were adopted to detect the expression of kalirin-7 and kalirin-7 mRNA in the peri-ischemic brain tissues respectively.Results The average Zausinger scores of the model group and 2 training groups were significantly lower than that of the sham-operation group at all time points;on the 7th and 14th days,the average score of the intensive training group was significantly higher than that of the outine training group.Moreover,the expression of kalirin-7 and kalirin-7 mRNA in the MCAO rats were significantly lower than that in the sham-operation group.On days 7 and 14,the expression of kalirin-7 in the intensive training group was significantly higher than that of the routine training group,which was still significantly higher than the model group.On day 14,the expression of kalirin-7 mRNA in the intensive training group was significantly higher than that of the routine training group,which was still significantly higher than the model group.Conclusion The intensive training is superior to routine training in promoting neural functional recovery and expression of kalirin-7 in rats with cerebral ischemia-reperfusion.

Objective: To probe the effects of gross polysaccharide of Radix Rehmanniae Preparata(GPRRP) on the morphology changes of immune organ of blood deficiency model mice.Methods: Using the blood deficiency mice models induced by releasing blood and injection of Cyclophosphamide as the experimental objects,the model animals were divided into 5 groups,that was the large,middle and small dose of GPRRP groups,Danggui Buxue Oral Liquid group,model group,at the same time,giving a blank control group.It was given successively relating drugs to the animals of several groups for 10 days.At the end of the experiment,the hemogram was measured and the thymus and spleen were taken,and making pathological section,then these tissues morphology changes were observed under microscope.Results: GPRRP can obviously resist the atrophy of thymus and spleen induced by the model establishing,can obviously increase the depth of thymus cortex and the number of cortex cells,can enlarge the size of spleen nodules and obviously increase the number of lymphocyte.Conclusion: GPRRP can resist the atrophy of thymus and spleen in blood deficiency model mice.

Objective To investigate the relationship between OHSS and FSHR gene polymorphism. Methods Two hundred and two women were enrolled in this study. The FSHR gene polymorphisms at position 307 and 680 were detected. Results The distribution of the allele frequency and genotype frequency of the position 680 in FSHR gene were significantly different between women with OHSS or not. No significant differences of the position 307 in FSHR gene were observed. Conclusion Themutation of Asn680Ser in FSHR might be closely related with OHSS.

Artery cannulation is one of the clinical skills that should be mastered by the internships of anesthesiology. In consideration of its invasiveness,teachers should carry out the clinical teaching strictly and patiently,and assist the internships to establish a correct opinion on clinical practice. We should train the internships step by step,improve their success rates on artery cannulation and avoid complications as far as possible.

AIM:To establish an HPLC method for determining the contents of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd. METHODS:The samples were separated on an Alltima C 18 (250 mm? 4.6 mm,5 ?m) column with the mobile phase of MeOH(A)-0.5% glacial acetic acid solution;gradient elution(0～15 min,30%～60% A;15～30 min,60%～60% A).Flow rate was 1.0 mL/min. The detection wavelength was set at 350 nm.Column temperature was at 30 ℃. RESULTS:The contents of caffeic acid,quercetin and campherenol were 14.218～23.695 ?g/g,9.919～25.564 ?g/g and 6.229～18.160 ?g/g in Hedyotis diffusa Willd from different sources. The linear range of caffeic acid was 0.005 0～0.200 0 ?g(r=0.999 9),the average recovery was 102.35%,RSD was 2.31%(n=6);The linear range of quercetin was 0.006 2～0.244 0 ?g(r=0.999 9),the average recovery was 101.84%,RSD was 1.79%(n=6);The linear range of campherenol was 0.007 8～ 0.310 6 ?g(r=0.999 9),the average recovery was 99.04%,RSD was 2.90%(n=6). CONCLUSION:The method for quantifying of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd is accurate and reliable.