OBJECTIVE:To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of MMPs. METHODS:Treated with 0(blank control),5,10,20,40,80,160,320 μg/ml baicalin for 24 h,the survival rate,the expression amount of MMP-2 and MMP-9 were detected,and IC50 was calculated. Cell apoptosis was observed. RESULTS:Com-pared with blank control group,after treated with baicalin,survival rate of MG63 cells decreased,while apoptotic amount in-creased,the expression amount of MMP-2 and MMP-9 decreased;there was statistical significance in the expression amount de-crease of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin(P<0.05);IC50 was(40.21±9.20)μg/ml,all responses were in concentration-dependent manner. CONCLUSIONS:Baicalin can inhibit the proliferation of MG63 cells,induce cell apoptosis,and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.

Pachychoroid spectrum diseases includes central serous chorioretinopathy (CSC),pachychoroid pigment epitheliopathy,pachychoroid neovasculopathy,and polypoidal choroidal vasculopathy,which share common characteristics,including focal or diffused increased choroidal thickness,choroidal hyperpermeability,and dilated choroidal vessels.These diseases are likely to represent a continuum of the same pathogenic process.Similar features and association among them suggest that they may have similar etiology.It is of great clinical significance to understand the composition and typical morphological changes of pachychoroid-related diseases and to explore its possible pathogenesis.

0 05), respectively.Conculsion Transduction of NKG 5SV into NK cell can augment its cytotoxic activity by enhancing its cytolytic ability.

Objective To observe the effects of shenfu injection (SFI) on the awakening quality of the patients with hepatitis B cirrhosis undergoing splenectomy under general anethesia. Methods Forty patients with hepatitis B cirrhosis and hepatic insufficiency (ASA classⅡ~Ⅲ) underwent splenectomy by general anesthesia. Patients were all sent into the post-anesthesia care unit (PACU) shortly after the operation with unconscious and no spontaneously breathing. They were randomly divided into two groups: SFI treatment group (Group SFI, n =20) and normal saline controlled group (Group NSC, n = 20). SFI group were treated with SFI (1 mL/kg, i. v.) in 10 minutes, and NSC group were treated with normal saline (1 mL/kg,i.v.). The time of eyes opening, extubation of tracheal catheter and the detention time of PACU were recorded. The heart rate (HR) and the average artery presses (MAP) were monitored at 4 time points: before SFI and normal saline administration, 5 min, 15 min, and 45 min after administration. The incidence of restlessness during the patients awakening period was also recorded. Results The time of eyes opening, extubation and the detention time of PACU of SFI group show no significant difference compared with the NSC group (P > 0.05). SFI and normal saline intravenous injection did not cause significant changes on HR and MAP at the time of 5 min , 15 min and 45 min compared to the time of before administration (P > 0.05). The incidence of restlessness during the patients resuscitation period in SFI group were lower than in NSC group (P < 0.05). Conclusion Shenfu injection can effectively improve the awakening quality and decrease the incidence of restlessness of the patients with hepatitis B cirrhosis undergoing splenectomy under general anesthesia during the awakening period in PACU.

Objective To analyze molecular classification and male breast cancer (MBC) triggers,clinical characteristics in patients with MBC and evaluate the prognosis.Methods 36 MBC patients enrolled in the past 5 years were retrospectively analyzed.100 patients with women breast cancer (WBC) in the same period were randomly selected as control group.The primary pathological type of MBC was the infiltrating ductal carcinoma.According to the expressions of estrogen receptor (ER),progesterone receptor (PR),and the epidermal growth factor receptor 2 (Her-2),the MBC can be divided into 4 kinds of molecular subtypes,Luminal type A,Luminal type B,Her-2 type,and Basal-like type.Results The ratio of MBC patients with grade Ⅲ was lower in the Luminal group A than that in the Luminal group B.There were significant differences (x2 =1.197,P ＜ 0.05) between these two groups.Conclusion The incidence rate of MBC is low but the prognosis is poor.The primary pathological type is the infiltrating ductal carcinoma.The ratio of MBC patients with grade Ⅲ is lower in the common Luminal group A than that in the common Luminal group B.

Objective To explore the correlation between serum uric acid levels and uric acid excretion indexes in children with primary nephrot?ic syndrome(PNS),and to reveal the prevalence of hyperuricemia and its possible causes in PNS. Methods The clinical data in 74 cases of pe?diatric PNS were retrospectively analyzed. These patients were grouped as hyperuric acid(HUA)or non?hyperuric acid(NUA),and their clinical features and laboratory findings were compared between two groups,including age,serum uric acid,serum creatinine,urea,cystatin C,triglycer?ide,cholesterol,albumin,creatinine clearance rate,24 hours urine uric acid,serum uric acid/serum creatinine,uric acid clearance,and the frac?tional excretion of uric acid and other indicators of differences. In addition ,a correlation analysis was carried out for the excretion of uric acid index. Results Several factors were lower in HUA group than that of NUA group ,such as the serum albumin and fractional excretion of uric acid(P1=0.034,P2=0.025);while the serum cystatin C and serum uric acid/serum creatinine ratio(P1=0.038,P2=0.001)was higher in HUA group. The uric acid excretion was negatively correlated with serum uric acid levels in all 74 children with PNS(r=-0.43,P=0.016),and the serum uric acid/creatinine ratio was positively correlated with serum uric acid(r=0.486,P=0.001). Conclusion Both the increase of blood uric acid production and excretion reduction were observed in children with PNS ,and the serum uric acid levels and renal tubular function is closely related.

Introduction of problem-based learning (PBL) teaching is the demand of teaching reform.Teaching and research section of physiology in Chongqing medical university implemented PBL teaching throughout the whole process of clinical medicine undergraduate education.The overall quality of students and the teaching quality were improved by constructing a new mode suitable for physiology teaching,focusing on training students' active learning ability and stimulating students' enthusiasm for learning.

BACKGROUND: Traditional cancellous bone porosity detection methods such as direct method,gas expansion method,mercury injection method,density and osmotic suction measurement have many disadvantages,such as low efficiency,labor-intensive and complexity.OBJECTIVE: To process cancellous bone chips using MATLAB image processing method to calculate cancellous bone porosity.METHODS: Using MATLAB image processing method,the stained cancellous bone images were subjected to uneven lighting removal,image enhancement,binarization,removing interference objectives to obtain binary two-dimensional matrix.RESULTS AND CONCLUSION: Slice image was composed of bone and pore space.The slice image was segmented to calculate its porosity.This solves the problem of the inequality of light intensity and other interferences in image information that affect the statistics.The image processing and analysis methods of bone slices were performed using MATLAB,which can be used for cancellous bone porosity measurements and be used to explore the distribution and variation of porosity.Selecting different regions to calculate the different porosity of each area can be used to analyze the distribution of the pore.This method provides a method for the study of trabecular bone structure.

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