1.Chronic Severe-degree Hepatitis B Complicated by Aspergillosis:Analysis of Clinical Characteristics
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To evaluate the clinical characteristics of chronic severe-degree hepatitis B complicated by aspergillosis and its susceptive factors and prevention and treatment.METHODS The clinical data of 3 patients with chronic severe-degree hepatitis B complicated by aspergillosis were analyzed retrospectively.RESULTS From 23 patients with chronic severe-degree hepatitis B complicated by fungus infection,there were 3 patients complicated by aspergillosis and died.The susceptive factors included poor immunity,incorrect use of antibiotis and invasive operation.CONCLUSIONS Patients with chronic severe-degree hepatitis B complicated by aspergillosis have poor progress and prognosis. The effective preventive methods are treating underlying disease actively,using antibiotis correctly,reducing or avoiding invasive operation and disinfecting air regularly.
2.Analysis of 1 372 Cases of Adverse Drug Reaction Reports in 36 Hospitals in Xi’an
China Pharmacy 2015;(26):3669-3671
OBJECTIVE:To investigate the characteristics and regularity of ADR in secondary-level hospitals and above in Xi’an in order to provide reference for clinical drug use. METHODS:During Oct. 2012-Jun. 2014,1 372 ADR cases reported in 36 hospitals at secondary-level or above in Xi’an were analyzed retrospectively. RESULTS:Among 1 372 cases of ADR reports,the incidence of ADR in the above 60 age group occupied the highest percentage,being 29.67%(407 cases);ADR reports were most likely caused by antibacterial drugs,accounting for 45.77%,followed by TCM injections(15.82%)and circulatory system drugs(9.55%);the largest number of ADR reports was caused by injections(1 210 cases,88.19%);the main types of ADR were lesion of skin and its appen-dents,accounting for 44.16%. The most number of ADR was induced by Levofloxacin injection,involving 95 cases. CONCLU-SIONS:Mastering the characteristics and regularity of ADR help managers to standardize clinical rational drug use and ensure patients’ medication safety.
3.Effect of Xinmailong injection combined with Qiliqiangxin capsule on serum BNP, NF-kappa B and cystatin C in elderly chronic heart failure
Chinese Journal of Biochemical Pharmaceutics 2016;36(11):147-150
Objective To investigate the effect of Xinmailong injection combined with Qiliqiangxin capsule on serum BNP , NF-kappa B and cystatin C in the treatment of chronic heart failure in the elderly .Methods 78 cases of elderly patients with chronic heart failure were selected and randomly divided into two groups according to the random number table, 39 cases in each group.All patients received basic treatment, the control group received standard treatment, and the study group received more with Xinmailong injection combined with Qiliqiangxin capsule,and levels of serum B-type natriuretic peptide(BNP), cystatin C(CysC) and NF-κB were compared, left ventricular function and 6 min walking test were detected and the clinical curative effect were compared after the treatment.Results Compared with before treatment, levels of serum BNP, CysC and NF-κB in 2 groups decreased(P<0.01), left ventricular ejection fraction(LVEF) increased(P<0.05), left ventricular end-systolic volume(LVESV) reduced(P<0.05), 6 min walking distance increased(P<0.01), scores of symptom decreased(P<0.01), compared with the control group, levels of serum BNP, CysC and NF-κB in study group were lower, LVEF were higher ( P<0.05 ) , levels of LVEDV and LVESV were lower ( P<0.05 ) , scores of 6 min walking distance were higher(P<0.01), scores of symptom were lower (P<0.01), the effective rate was higher(P<0.05).Conclusion Xinmailong injection combined with Qiliqiangxin capsule in the treatment of chronic heart failure in the elderly was effective , and it can improve left ventricular function, the possible mechanism related to the decrease of BNP, CysC and NF-κB levels.
4.Expression and Purification of Hi-lys Peptide,a Recombinant Relevant to Hirudin
Jie WU ; Zhuoyi HU ; Jingjing LIU
Chinese Journal of Biochemistry and Molecular Biology 2005;21(3):287-291
A new fusion expression vector, pED-P8-Hi-lys was designed and constructed. It includes four parts, a 20 peptide sequence of hirudin that can maintain anticoagulant activity, the C-terminus of asparaginase as a fusion partner, basic octopeptide (KRKRKKSR) that makes the fusion partner easy to remove, and the unique acid-labile aspartyl-prolyl bond. It was transformed into E. coli BL-21 and the fusion protein (AnsB-C-P8-Hi-lys) was expressed effectively as inclusion bodies after inducing by lactose. The objective peptide Hi-lys was purified by means of cell disruption, washing, ethanol precipitation, acid hydrolysis, and DEAE-cellulose 52 column chromatography. The antithrombin activity of the purified Hi-lys peptide was about 50 ATU/mg by thrombin activity assays.
5.From“Treating Illness”to“Treating Patients’Illness”
Shengjie HU ; Jingjing HU ; Juan WU ; Bein LIU
Chinese Medical Ethics 1995;0(04):-
With the gradual development of reformation,the traditional notion of service that only cares about the state of illness but ignores the state of patients is challenged by different factors violently in the hospital.Nowadays,with more concerns on People First,the situation,neglect of patients,must be accordingly changed in hospitals and more em?phasis should be put on the humane care during treatment in order to cure patients’diseases successfully.
6.Effects of magnetic field on the behaviors of depression rat model
Jia LIU ; Lisha HAN ; Hai HU ; Yuting MA ; Jingjing JI
Chinese Journal of Physical Medicine and Rehabilitation 2013;35(10):760-762
Objective To study influences of magnetic field on behavior of depressive rats under chronic unpredictable mild stress,and possible mechanisms under such phenomena.Methods Thirty-six Wistar rats were divided into model group,magnetic field group and control group (n =12 in each group) according to random number table.The model group and the magnetic field group received CUMS stimulation daily for 5 weeks,while the control group received no any treatment.At the end of the fifth week,rats in the magnetic field group were placed in the center of a magnetic machine (400 mT),and the model group was raised freely for4 weeks.The severity of depressive behaviors,cortisol levels and contents of C-reactive protein in blood and urine sample were tested pre-treatment,at the end of 5 weeks and 9 weeks.Results After 5 weeks,consumption of glucose solution of the control group was higher than the other two groups (P < 0.05).After 5 and 9 weeks,body weights of the magnetic group and the model group were lower than the control group (P < 0.05).At the end of 9 weeks,performance of the magnetic group as indicated by frequency of grid crossing,grooming time and suspending tail test were improved to a significantly greater extent than the other two groups (P < 0.05).In addition,the urine content of cortisol in 24 hours in magnetic field and model groups was higher than the control group at the end of 5 weeks,and the serum level of cortisol in the magnetic field group were significantly higher than the model group at the end of 9 weeks (P < 0.05).Conclusion The 400mT magnetic field can reduce the severity of behavior changes of rats with CUMS-induced depression,which might be attributable to its influence on the cortisol levels in blood and urine.
7.Relationship between acceptance of disability and posttraumatic stress response in patients with brachial plexus injury
Ni CHEN ; Yun CHENG ; Xiaochun SUN ; Jingjing HU
Chinese Journal of Nursing 2009;44(8):677-680
Objective To determine the relationship between acceptance of disability and posttraumatic stress response in patients with brachial plexus injury. Methods Total 160 patients with brachial plexus injury were recruited and investigated with the Impact of Event Scale-Revised (IES-R) and the Acceptance of Disability Scale (ADS). Results The total score of ADS was (79.07±11.99) which showed medium level of acceptance of disability. The total score of IES-R was 4-66 (33.51±14.41), which showed that most of the patients suffered from posttranmatic stress response. Significantly negative correlation was found between acceptance of disability and posttraumatie stress response(r=-0.480, P<0.001). Conclusions Nurses should pay more attention to the acceptance of disability and posttranmatic stress response in patients with brachial plexus injury, and provide appropriate health education and effective psychological intervention to improve the patients' mental health and quality of llfe.
8.Cloning of D-hydantoinase Gene from Pseudomonas and Its Expression in E.coli
Zhiqiang LI ; Jingjing LIU ; Zhuoyi HU ; Zhenghua WANG ; Xin MING
Journal of China Pharmaceutical University 2001;(3):227-230
AIM The purpose is to construct D-hydantoinase genetic engineering strain for the purpose of the industrial production of D-p-hydroxyphenylglycine. METHODS D-hydantoinase gene was created from Pseudomonas putida 9801 by PCR technique and inserted into pMD18-T vector. The recombinant plasmid was transformed into several Escherichia coli strains. The positive transformants with D-hydantoinase activity were obtained by the two step screening, digoxigenin DNA labeling in situ hybridization and D-hydantoinase activity assay. RESULTS The D-hydantoinase activity of the genetic engineering strain E.coli BL21/pMD-dht was 1700 U*L-1 and increased as high as 8 times compared with those of wild-type strain Pseudomonas putida 9801. The subunit molecular weight of recombinant D-hydantoinase was about 53 kDa measured by SDS-PAGE. The amount of the recombinant D-hydantoinase was about 20 percent of total bacterial soluble proteins. CONCLUSION The genetic engineering strain E.coli BL21/pMD-dht possesses the initial industrial production prospects.
9.Construction and screening of plasmid expression vectors encoding short hairpin RNA targeting TWIST gene
Bin SHEN ; Jinghai HU ; Jingjing GUAN ; Chunxi WANG
Journal of Jilin University(Medicine Edition) 2006;0(01):-
Objective To construct a plasmid expression vector coding for the short hairpin RNA(shRNA) targeting Twist mRNA.Methods Two plasmid expression vectors coding for shRNA targeting 777 and 845 of Twist gene sequence and a control vector containing random DNA fragment were constructed.The recombinant plasmids were identified by PCR,and then transfected separately into bladder cancer cell line T24.The Twist gene silencing effect was detected by RT-PCR and Western blotting.Results The expected band of 400 bp was amplified from the plasmids coding for shRNA by PCR.By DNA sequencing,it was the same with the insertion element as with the shRNA of synthetic.Transfection of T24 cells expressing Twist gene with the shRNA plasmids resulted in inhibition of Twist mRNA and protein expressions by 90% and 86%,respectively.The shRNA1 had the most obvious effect in Two types of plasmids interference.Conclusion The plasmid expression vectors coding for shRNA targeting Twist mRNA have been constructed successfully,of which pGenesil-shRNA1 most effectively silences Twist gene in T24 cells.
10.Early assessment of the level of serum cystatin C to renal damage in patients with multiple myeloma
Lingling SUN ; Yanli MENG ; Guangyu SONG ; Jingjing HU ; Bin JI
Chinese Journal of Postgraduates of Medicine 2014;37(25):56-58
Objective According to the level of serum cystatin C (Cys-C)in patients with multiple myeloma (MM),to explore its significance of early assessment to renal damage.Methods To select 42 patients with MM(MM group) and 30 healthy subjects(control group),and measure serum Cys-C,creatinine (SCr) and β2-microglobulin (β2-MG).Results The level of serum Cys-C in MM group was significantly higher than that in control group [(2.34 ± 0.57) mg/L vs.(0.66 ± 0.12) mg/L],which increased with ISS stage[Ⅰ,Ⅱ,Ⅲ stage was(1.77 ± 0.38),(2.11 ± 0.46),(3.14-± 1.07) mg/L](P< 0.05).Cys-C and β 2-MG,renal function had a positive correlation (r =0.760,0.694,P < 0.05).Conclusion The increasing of Cys-C in MM patients is related to impaired renal function and tumor burden,which can be used as an early assessment indicators.