1.The epidemiological study of the incidence in encephalopathy of prematurity
Chinese Journal of Applied Clinical Pediatrics 2015;30(10):796-798
With the neonatal intensive care techniques developing,the survival rate of preterm infants has increased markedly.However,these survival preterm infants are extremely vulnerable to develop various diseases,of which brain injury is the most important cause of the disability in preterm infants.At present,encephalopathy of prematurity,the incidence of which is rarely reported in domestic and national reports,mainly refers to white matter injury (WMI).This article reviews the different domestic and national reports about the incidence of encephalopathy of prematurity.
2.The mediation effect of psychiatric nurses' communication ability on the relationship between personality characteristics and conflict management patterns
Jingjing CHAO ; Feifei SUN ; Qinghua LU
Chinese Journal of Practical Nursing 2021;37(6):454-459
Objective:To understand the relationship between psychiatric nurses' communication ability, personality characteristics and conflict management patterns, and to provide a basis for training psychiatric nurses' conflict management ability courses.Methods:A convenient sampling method was used to investigate and investigate 208 nurses in the first-line clinical hospitals of a third-class psychiatric hospital, used the nurses′ clinical communication ability scale, the nurse′s interpersonal conflict management morphology scale, and the simplified version of the Chinese Big Five Personality Questionnaire.Results:Integration dimension (18.67±2.58) points, concession dimension (12.93±2.43) points, escape dimension (14.05±2.02) points, dominance dimension (14.49±3.06) points, and integration dimension and gender ( t value was -2.57, P=0.01)), education ( F value was 3.76, P=0.02), technical title ( F value was 3.03, P=0.02), position ( t value was 2.08, P=0.03), whether to participate in nurse-patient communication training ( t value was 2.54, P=0.02). Nurses with pleasant personality could better deal with interpersonal conflict ( r=0.361, P<0.01), and the nurse′s personality had a direct effect on the form of interpersonal conflict ( P=0.036, 95% CI 0.107-0.422), the nurse′s communication ability plays a part of indirect effect ( P=0.031, 95% CI 0.001-0.063). Conclusion:The form of interpersonal conflict management of psychiatric nurses was closely related to communication ability and personality characteristics. Nursing managers could carry out targeted training to improve the conflict management ability of nursing staff.
3.Construction of a rabbit model of knee prosthesis infection:environmental factorsin vivo affect Staphylococcus epidermidis and biofilms
Youliang SHEN ; Tonge ZHU ; Jingjing ZHANG ; Chao QI ; Tengbo YU
Chinese Journal of Tissue Engineering Research 2015;(39):6240-6245
BACKGROUND:Bacterial biofilm is the main cause of the infection of the prosthesis.In vitro experiments confirmed that hypertonic sodium chloride and ethanol can apparently promote the formation of staphylococcal biofilms. There are no reports on the effects of ethanol and hypertonic environment surrounding the prosthesis on the formation of biofilms.
OBJECTIVE: To evaluate the effects of different environment factors surrounding the prosthesis on the growth of Staphylococcus epidermidis and bacterial biofilm formation after replacement.
METHODS: White rabbit models infected with Staphylococcus epidermidis on the prosthesis were established, and were randomly divided into hypertonic sodium chloride, ethanol and control groups (n=15). The bacteria were injected with 0.1 mL 4% sodium chloride and 4% ethanol into the knee of rabbits in the hypertonic sodium chloride and ethanol groups. The rabbits were injected with 0.1 mL 0.9% sodium chloride in the control group. Three rabbits were sacrificed at 2, 4, 6, 8 and 16 days after inoculated with bacteria. Synovial fluid, prosthesis and tissue surrounding infection were obtained. Bacterium was cultured to extract total RNA. The ica operon transcription levels were detected in the gene levels. Adhesion of bacteria on the surface of the prosthesis was observed using a scanning electron microscope. Tissues surrounding the prosthesis were observed using hematoxylin-eosin staining.
RESULTS AND CONCLUSION:Histological examination revealed that inflammatory cel infiltration was observed in al the rabbits at 4 days after injection. Colony formation was found at 16 days after injection. At 6 days after injection, inflammatory cel infiltration was observed in the ethanol and control groups. Scanning electron microscope showed that compared with the control group, the bacteria adhered to the prosthetic surface became more in the hypertonic sodium chloride and ethanol groups at 6, 8 and 16 days (P < 0.05). At 6, 8 and 16 days, the expression of icaA mRNA was significantly higher in the hypertonic sodium chloride and ethanol groups than in the control group (P < 0.05). These data showed that the environment factors could affect the growth of Staphylococcus epidermidis and bacterial biofilm formation.
4.Microfluidic cell chip method to analyze CD14+monocyte myeloperoxidase expression in acute myelomonocytic leukemia patients
Jingjing SONG ; Xiaoliang LI ; Jie LAN ; Chao SUN ; Peng GE ; Chengcheng HONG ; Xuguo SUN
Chinese Journal of Clinical Oncology 2014;(12):771-775
Objective:A method that is based on microfluidic cell chip technology was developed for the first time to analyze CD14+monocyte myeloperoxidase (MPO) expression in myelomonocytic leukemia (M4) patients. CD14+monocyte MPO expression in M4 patients was preliminarily discussed. Methods:a. The chip was prepared by using polydimethylsiloxane as the host material and by secondary foam molding. b. A total of 48 clinically diagnosed M4 patients and 52 patients with normal myelogram were included as the test and control groups, respectively. c. A method based on the microfluidic cell chip approach was established to detect CD14+mono-cytes and to determine the positive rate and degree of MPO expression in the cells. d. The microfluidic cell chip technique was used to compare CD14+monocyte MPO expression in M4 patients with that in the control. Results:a. The designed microfluidic single cell analysis chip allowed the entry of granulocytes into the corresponding microfluidic channels. Thus, blood cells were separated. Numer-ous ghost corpuscles surrounded the separated white blood cells (WBCs). WBC morphology did not show obvious changes. b. The posi-tive rate of MPO expression and the activity of CD14+monocytes in the bone marrow of M4 patients were significantly higher than those in the bone marrow of the control (P<0.05). Conclusion:A method based on microfluidic single cell technology was developed for the first time to analyze the MPO expression in CD14+monocytes. CD14+monocyte MPO activity in M4 patients was significantly higher than in the control. CD14+monocyte MPO activity can be used as an auxiliary examination marker for clinical diagnosis.
5.Advances in roles of NF-κB in regulating pathways of apoptosis
Gaiyan REN ; Aning SUN ; Jingjing ZHANG ; Chao DENG ; Zhengtao WANG ; Wei DOU
Chinese Journal of Pharmacology and Toxicology 2015;(2):323-327
Apoptosis is a process of programmed cell death that is controlled by genes. Normally, there are three regulation pathways involved in the process of apoptosis, including the signaling of intracel-lular mitochondria, endoplasmic reticula and extracellular death receptors. Recent studies showed that NF-κB is a key regulator in the process of apoptosis. NF-κB plays a promotional and a inhibitory role as well in the regulation of apoptosis, closely related to the the inhibitor of apoptosis proteins family, the B cell lymphoma/ lewkmia-2 family, tumor necrosis factor receptor associated factors, c-Jun N-terminal kinase, tumor necrosis factor related apoptosis inducing ligand and Fas-associated death domain protein-like interleukin-1β. Thus, investigation of the mechanism regarding NF-κB in apoptosis regulation is of great importance for apoptosis-related drug development. The paper reviews the recent research progress in the function of NF-κB in apoptosis pathway regulation.
6.The inhibition of laryngeal squamaous cell carcinoma by endostatin
Dejun JIN ; Yu WANG ; Hui XIAO ; Chao WANG ; Jingjing HE ; Xinghan LIU
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(05):-
OBJECTIVE To evaluate the inhibitory effect and possibility inhibitory mechanisms ofendostatin on laryngeal carcinoma cells (Hep-2) and human umbilical vein endothelial cells (HUVEC). METHODS ①Using the MTT assay , the effect of different concentrations of endostatin (10 ?g/ml~50 ?g/ml) and endostatin (30 ?g/ml) with different contact times (24~72h) on the livability of Hep-2 and HUVEC cells were determined; ②Changes in the ultrastructures of Hep-2 and HUVEC cells were examined by electron microscopy; ③Survivin mRNA content was determined in Hep-2 cells exposed to endostatin (30 ?g/ml) by RT-PCR test; ④The effect of endostatin (30 ?g/ml) on the ectogenetic artificial blood vessel models was observed by light microscopy. RESULTS ① The growth of Hep-2 and HUVEC cells was significantly inhibited (P
7.Effects and mechanisms of vitexin against ulcerative colitis in mice
Aning SUN ; Gaiyan REN ; Chao DENG ; Jingjing ZHANG ; Zhengtao WANG ; Wei DOU
Chinese Pharmacological Bulletin 2014;(12):1677-1680,1681
Aim To evaluate the effect and mecha-nism of vitexin in a mouse model of DSS-induced ulcer-ative colitis (UC).Methods C57BL/6 mice were randomly placed into three groups: normal control group,DSS group and DSS +Vitexin group.Mice coli-tis was induced by adding 4% dextran sulphate sodium (DSS)into the drinking water for seven days.Vitexin was administered once a day along with DSS treatment. Mice were monitored daily with body weight change and diarrhea symptoms.After sacrifice,colon was re-moved and fixed in 1 0% (W/V)buffered formalin for hematoxylin-eosin (H&E)staining.Histological dam-age was assessed as a combined score of inflammatory cell infiltration and mucosal damage.The remaining colon pieces were collected to measure the activity of myeloperoxidase (MPO)by ELISA method and to de-termine the mRNA expression of TNF-α,IL-6 and COX-2.Results None of the mice receiving vehicle alone exhibited body weight loss and mucosal disrup-tion at any point during the study.Vitexin treatment significantly ameliorated DSS-induced body weight loss and histological score.The activity of MPO and the mRNA expression of TNF-α,IL-6 and COX-2 were markedly inhibited by vitexin treatment.Conclusion Vitexin ameliorates DSS-induced colitis through sup-pressing leukocyte infiltration and pro-inflammatory mediators production.
8.Effect and mechanism of baicalein on 2,4,6-trinitrobenzene sulfonic acid-induced experimental colitis of mice
Xiaoping LUO ; Zhilun YU ; Chao DENG ; Jingjing ZHANG ; Zhengtao WANG ; Wei DOU
Chinese Journal of Pharmacology and Toxicology 2017;31(6):541-546
OBJECTIVE To explore the effect and mechanisms of baicalein on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis in mice.METHODS BALB/c mice were randomly placed into three groups (n=10):normal control group,TNBS group,and TNBS+baicalein (20 mg· kg-1,once per day) group.Mouse colitis was induced by intrarectal injection of TNBS.Baicalein was administered by oral gavage two days prior to TNBS treatment and until the end of the study (a total of 9 d).The colon length was measured before HE staining was performed for histological damage assessment.The remaining colon pieces were collected to measure the content of tumor necrosis factor-α(TNF-α).Lipopolysaccharide (LPS)-stimulated RAW264.7 mouse macrophage was used as a cell model to determine the content of nitric oxide (NO) in cell culture medium,the mRNA levels of TNF-α,interleukin-6(IL-6),IL-1β,inducible nitric oxide synthase(iNOS),cyclooxygenase 2(COX-2) and monocyte chemoattractant protein-1 (MCP-1),and the protein expression of phosphatidylinositol 3-kinase/protein kinase B/nuclear factor-κB (PI3K/AKT/NF-κB) pathway.RESULTS Baicalein significantly attenuated TNBS-induced colon shortening and histological injury (P<0.05),which was correlated with the decline in the content of TNF-α in the colon.According to the jn vivo results,baicalein exposure down-regulated the secretion of NO and the mRNA expression of pro-inflammatory mediators (iNOS,COX-2,MCP-1,TNF-α,IL-1β and IL-6) in LPS-stimulated RAW264.7 cells (P<0.05,P<0.01).Additionally,the phosphorylation/activation of LPS-stimulated PI3K/AKT/NF-κB pathway was inhibited by baicalein treatment.CONCLUSION The beneficial effect of baicalein in TNBS-induced experimental colitis may be due to PI3K/AKT/NF-κB signaling inhibition.
9.Glycemic excursion-induced islet-like cells in rat bone marrow
Xiaohong WU ; Jian ZHU ; Jingjing JIANG ; Yu XU ; Cuiping LIU ; Xiaodong MAO ; Kuanfeng XU ; Chao LIU
Chinese Journal of Endocrinology and Metabolism 2008;24(2):208-209
Glycemic excursion was induced in SD rats by intraperitoneal injection of 50% glucose solution, and cells isolated from bone marrow of these rats showed cell clusters which expressed insulin, c-peptide, glucagon, somatostatin and islet amyloid polypeptide, and other genes related to islet-cells development and functions.
10.Relationship between schizotypal personality proneness and intelligence
Junhua TANG ; Shengjun WU ; Xufeng LIU ; Yebing YANG ; Jianquan TIAN ; Jingjing TANG ; Chao HE ; Danmin MIAO
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(2):130-132
Objective To explore the relationship between schizotypal personality proneness and intelligence .Methods 1905 subjects were tested by Chinese Soldier Personality Questionnaire(CSPQ).According to the results of CSPQ,the subjects were divided into high-risk subjects and normal ones.The Words Reasoning Test, the Arithmetic Reasoning Test and the Assembling Test were used to evaluate the intelligence,and the scores of these tests were compared between the two groups.Correlation coefficient between schizotypal personality proneness and intelligence was calculated.Results The scores of the Words Reasoning Test,the Arithmetic Reasoning Test and the Assembling Test in high-risk subjects were significantly lower than those in normal ones((87.83±18.42)VS(101.37±13.48),P<0.01;(91.74±14.26)vs(101.65±14.62),P<0.01;(87.70±18.82)VS (101.73±14.26),P<0.01).Schizotypal personality proneness was significantly correlated with the scores of intelligence(r=-0.39,P<0.01).Conclusion Lower intelligence level is found in the group with schizotypal personality proneness.Intelligence is correlated with schizotypal personality proneness.