BACKGROUND: Umbilical cord blood mesenchymal stem cells (UCB-MSCs) can differentiate into various types of cells under certain condisions, and easily proliferate in vitro. However, UCB-MSCs have long establishment time and low frequency.OBJECTIVE: To in vitro isolate and culture UCB-MSCs, and induce its differentiation into osteoblasts.DESIGN, TIM E AND SETTING: The in vitro cytological study was performed at the Laboratory of the Medical College of Qingdao University from June 2008 to January 2009.MATERIALS: UCB was obtained from term normal delivery women at the Department of Gynaecology and Obstetrics, Qingdao Municipal Hospital.METHODS: Human UCB-MSCs were isolated and cultured in vitro by Percoll density gradient. When reached 90% confluency,UCB-MSCs were digested by trypsin for subculture. At the third passage, UCB-MSCs at 1×106 were incubated. When reached 50% 60% cenfluency, UCB-MSCs were treated with DMEM supplemented with 0.1 μmol/L dexamathasone, 10 mmol/Lβ-sodium glycerophosphate and 50 μmol/L vitamin C. UCB-MSCs in the control group were incubated in low glucose DMEM.MAIN OUTCOME MEASURES: Growth and proliferation of MSCs were observed under the inverted microscope. Cell surface marker expression and cell growth curve were measured by flow cytometry. Cell ultrastructure was observed under the transmission electron microscope. Differentiation of UCB-MSCs into osteoblasts was determined by Won Kossa staining and alkaline phosphatase staining.RESULTS: Primary cultured UCB-MSCs had similar morphology to bone marrow mesenchymal stem cells. After passage, cell morphology was even, presenting spindle shape. UCB-MSCs at passage 3 highly expressed CD29, CD44, CD13, but did not express CD34. Growth latency was 2-3 days. Cells entered logarithm proliferation phase at days 3-4, and platform phase 1 month later. Nuclei presented round or irregular, with clear nuclear membrane, 1-2 nucleoli, rough chromatin, abundant organelles and microvilli. UCB-MSCs at passage 3 were gradually confluent following 3 days of osteogenic induction, with the presence of pavement-stone shape. 14 days later, calcified nodules by Von Kossa staining, and cells were positive for alkaline phosphatase staining. In the control group, no calcified nodules were found, and cells were negative for alkaline phosphatase staining.CONCLUSION: UCB-MSCs can be successfully isolated by Percoil density gradient, and induced to differentiate into osteoblasts in vitro.

Objective To investigate the unilateral spatial neglect (USN) on the rehabilitation of hemiplegia after brain injury.Methods 35 patients with USN and 38 patients without USN were selected and divided into two groups (USN group and without USN group). All patients of two groups accepted integrative treatments mainly with Bobath technique by professional therapists. The function of movement, balance, ambulation, hand and activities of daily living (ADL) were all particularly assessed by simplified Fugl-Meyer movement assessment, Fugl-Meyer balance assessment, Holden functional ambulation classification, hand function classification and Modified Barthel Index (MBI) before treatment and 1 and 2 months after treatment.Results After treatment, the scores of the Fugl-Meyer movement assessment, Fugl-meyer balance assessment and MBI of all patients in two groups significantly improved ( P<0.001). But there was a significant difference between the two groups in scores of balance function, MBI, hand function (all P<0.05), and the Holden functional ambulation classification ( P<0.001).Conclusion USN induced by brain injury has obviously effects on rehabilitation of motor, balance, ambulation and ADL of hemiplegia.

Objective To observe the effect of ankle foot orthoses (AFO) on improving the abnormal gait pattern in hemiparetic patients Methods 106 hemiparetic patients were randomly divided into two groups. AFO was used in one group (52 cases) and not in the control group (54 cases). Both of them accepted conventional physical therapy. Motor function of affected ankle joint and lower extremities, spastic state of ankle joint, standing balance and walking ability were evaluated on each case before and after rehabilitation Results At the end of the trial, spastic state of ankle joint in both groups were decreased. 40 cases reached to degree of ⅠorⅠ + of spastic state and 12 cases to Ⅱ, Ⅲ or Ⅳ in the AFO used group,while the number of cases in the control group were respectively 27 and 27. The effects in AFO used group showed more significant than in the control group ( P 0.05) Conclusion Physical therapy combined with ankle foot orthoses was beneficial to improving the abnormal gait pattern and decreasing the spastic state of ankle joint in hemiparetic patients. However, it might not affect the motor function of ankle joint and lower extremities.

The traumatic brain injury(TBI)is the main cause of death and disability after earthquake.The rehabilitation should intervene as earlier as possible.This paper would introduce some rehabilitation approaches practically.

Objective To analyze the clinical features and prognosis of central pontine myelinolysis. Methods 20 cases with CPM diagnosed in last 10 years were reviewed and compared with those patients of foreign studies. Results 10 patients with hemoptysis were treated with hypophysin, which resulted in severe hyponatremia and osmotic myelinolysis. The main syndrom were dysarthria and dysphagia (secondary to corticobulbar fiber involvement), flaccid quadriparesis (as corticospinal tract involved) which later became spastic. The myelinolysis distrubuted symmetrically along the myelin disruption in the center basal, as well as independently in other brain areas (extrapontine myelinolysis or EPM), including the cerebellar and neocortical white/gray junctional areas, thalamus and striatum. Conclusion Supplement of sodium should be slower and more persistent for hyponatremia, the selection of drugs should be based on the patients' specific conditions.

Objective To explore the effect of intensity of 1 Hz repetitive transcranial magnetic stimulation (rTMS) to right Broca's ho-mologue on nonfluent aphasia after stroke. Methods 40 inpatients were equally divided into low-intensity group and high-intensity group, who received rTMS (1 Hz for 10 minutes) once a day and twice a day respectively for 10 days. They were assessed with Western Aphasia Battery (WAB) before and after intervention, and 2 months after intervention. Results The scores of content and fluency, auditory compre-hension, naming, repetition and AQ improved more in the high-intensity group than in the low-intensity group (P<0.01). It maintained more for 2 months in the scores of content and fluency, repetition and AQ (P<0.05). Conclusion High-intensity rTMS can promote the improve-ment of speech in patients with nonfluent aphasia after stroke that remains for a long term.

Objective To improve the quality standard of compound Chaihu tea. Methods Pupleuri Radix, Puerariae Lobatae Radix and Forsythiae Fructus were identified by thin layer chromatography (TLC).HPLC method was used to determine the content of puerarin.The determination was performed on a Shimadzu VP-ODS-C18column (250 mm×4.6 mm,5μm) with the mobile phase of methanol-0.2% H3PO4(23:77).The flow rate was 1.0 mL·min-1, the column temperature was set at 30 ℃ and the detection wavelength was 250 nm. Results The TLC spots were clear and well-separated without negative interference.The good linear correlation existed within the range of 1.340 8-56.632 8μg· mL-1 for puerarin (r=0.999 9, n=6) and the average recovery was 102.98% with RSD being 1.48% (n=6). Conclusion The method is simple and accurate.It can be used for quality control of compound Chaihu tea.

Objective To explore the characteristics and rehabilitation treatment methods of young ischemic stroke combined with congenital myopathy. Methods The clinical data of one young patient with ischemic stroke combined with congenital myopathy was retrospective analyzed, and its pathological feature and rehabilitation treatment methods were also analyzed. Results The etiology may be artery dissection mural thrombus fell off. Conclusion The etiology of young ischemic stroke should be clear diagnosed, and the intensity of rehabilitation training need attention.

A 33-year-old male patient with tick-borne encephalitis (TBE) was reviewed, who presented with severe neurological deficits following TBEV infection, and improved in his motor and quality of life after an individualized rehabilitation.

Objective To evaluate quality control circle (QCC) impacting on Burnout syndrome of emergency nurse and satisfaction of patients in one hospital of Wenzhou.Methods Emergency nurse,who worked at one hospital of Wenzhou during January 2012 to December 2013,were chosen as sampling objects and divided into QCC group (47 nurses) and control group (39 nurses).The nurses of QCC group underwent QCC activities while nurses of the control group received routine management.The QCC activities lasted 3 months,and one week before and after QCC activities,we questionnaired Burnout syndrome,normal population sociology information,frequency of job burnout syndrome,work performance,and patient's satisfaction.Results Before activities,there was no statistical significance for occurrence of Burnout syndrome between two groups (P ＞ 0.05);one week after the implementation of activities,the fatigue,headache,insomnia,negative emotion had statistical significance between two groups (x2 =5.88,6.01,5.88,6.11,respectively;P ＜ 0.05),but the symptom of irritation were not statistical significant (x2 =0.52,P ＞ 0.05).Before activities,the work performance and patient's satisfaction had no statistical significance;one week after the accomplishment of activities,the scores of colleague relationship,work satisfaction,work efficient,patient's satisfaction for nurses in the QCC group were (5.81 ±0.19),(5.13 ±0.29),(5.12 ± 0.80),(5.33 ±0.54) higher than (4.90 ± 0.83),(4.73 ± 0.37),(4.01 ± 0.91),(4.48 ± 0.30) of the control group (t =2.613,2.433,2.695,2.497,respectively;P ＜ 0.05).The intention of turnover was (2.11 ± 0.98) score in the QCC group lower than that of control group (4.85 ± 0.71) score (t =2.946,P ＜ 0.01).Conclusions The QCC can promote the Burnout syndrome of emergency nurses,decrease nurse's intention to turnover,and enhance the enthusiasm nurse's work and patient's satisfaction.