The tutorial system is one of the most important characteristics of the Oxford University.Many graduates have become the elites of England including the prime ministers,government officials and famous scholars through this educational system.The tutors put a great deal of emphasis on the foster of the students' ability.It is“whole-person”education.The educational flow would be like this:self-study,independent thoughts,spreading learning and overall development.It has a great impact on our higher educational ideas,testing methods,quality of the tutors,the morality and the relationship between the tutors and students.It has enlightenment on the directors' system as well.

Hypoxia results from long-term anti-angiogenic therapy and can stimulate hypoxia-inducible factors (HIFs). HIF-induced hy-poxia signaling is involved in various steps in tumor invasive-metastatic cascade. On the one hand, HIFs regulate epithelial-mesenchy-mal transition. On the other hand, the characteristics of pericytes around vessels and the links among endothelial cells can change;thus, tumor cells can more easily intravasate into blood vessels, survive in peripheral blood, and then reach specific organs, ultimately resulting in metastasis. This review discusses the emerging mechanisms of long-term anti-angiogenic therapy and the occurrence of metastasis.

Objective: To investigate the awareness of adolescent sexual knowledge among primary school students in Hangzhou City, so as to provide insights into adolescent sex education. Methods: In May 2021, grades 5 and 6 students were sampled from 5 primary schools in urban areas and 5 primary schools in suburban areas of Hangzhou City. The demographic features, awareness of adolescent sexual knowledge and sources of acquiring adolescent sexual knowledge were investigated using electronic questionnaires, and the awareness of adolescent sexual knowledge were analyzed among primary school students. Results: Totally 1 812 students completed valid questionnaires, including 861 grade 5 students ( 47.52% ) and 951 grade 6 students ( 52.48% ), 936 boys ( 53.86% ) and 836 girls (46.14%), and 912 students living in urban areas (50.33%) and 900 students living in suburban areas ( 49.67% ). The awareness rates of adolescent physiological knowledge, adolescent health knowledge, private parts and sexual harassment behaviors were 53.20%, 46.30%, 60.49% and 92.55%, respectively, and the awareness rates of adolescent physiological knowledge ( 69.52% vs. 39.14%; χ2=168.001), adolescent health knowledge (55.74% vs. 38.22%; χ2=55.619 ), private parts ( 72.49% vs. 50.20%; χ2=93.550 ) and sexual harassment behaviors ( 96.05% vs. 89.55%; χ2=27.621 ) were significantly higher in girls than in boys ( all P<0.05 ). The awareness rates of adolescent physiological knowledge ( 63.72% vs. 41.58%; χ2=88.989 ), private parts ( 64.56% vs. 55.98%; χ2=13.926 ) and sexual harassment behaviors ( 93.80% vs. 91.17%; χ2=4.509 ) were significantly greater among grade 6 students than among grade 5 students ( all P<0.05 ), and the awareness rates of adolescent physiological knowledge ( 69.96% vs. 36.22%; χ2=207.041 ), adolescent health knowledge (58.99% vs. 33.44%; χ2=118.903 ) and sexual harassment behaviors ( 96.16% vs. 88.89%; χ2=34.754 ) were significantly higher among students living in urban areas than among students living in suburban areas ( all P<0.05 ). Primary school students mainly acquired sexual knowledge from their parents ( 1 208 students, 66.67% ) and teachers ( 1 161 students, 64.07%), and the source of life was the predominant demand for sexual knowledge ( 969 students, 53.48% ). Conclusions The awareness of adolescent sexual knowledge remains to be increased among grade 5 and 6 primary school students in Hangzhou City. More attention paid to male students and students living in suburban areas, and building a school-family-community educational network are recommended to increase the awareness of adolescent sexual knowledge among primary school students.

Objective To investigate the clinical efficacy of auricular plaster therapy for depression in newly diagnosed type 2 diabetes.Methods Sixty patients with depression in newly diagnosed type 2 diabetes were randomly allocated to group A of 18 cases, broup B of 22 cases and group C of 20cases. In addition to conventional hypoglycemic treatment, group A received psychological intervention; group B, auricular plaster therapy; group C, oral administration of fluoxetine hydrochloride capsules. Pre-/post-treatment changes in fasting blood glucose, two-hour postprandial blood glucose, glycosylated hemoglobin and the Hamilton Depression Scale (HAMD) score were observed in the three groups after eight weeks of treatment. The clinical therapeutic effects were compared between the three groups.Results There were no statistically significant post-treatment differences in fasting blood glucose, two-hour postprandial blood glucose and glycosylated hemoglobin among the three groups (P>0.05). There was a statistically significant pre-/post-treatment difference in the HAMD score in the three groups (P<0.05). There was a statistically significant difference in the HAMD score between groups B and A at two, four, six and eight weeks after treatment (P<0.05), between groups B and C at two weeks after treatment (P<0.05) and between groups C and A at two, six and eight weeks after treatment (P<0.05). The total efficacy rate was 77.8% in group A, 90.9% in group B and 90.0% in the control group. There was a statistically significant difference in the total efficacy rate between group B or C and group A (P<0.05).Conclusion Auricular plaster therapy plus medication is an effective way to treat depression in newly diagnosed type 2 diabetes.

[Objective] To establish a method for determination of Ephedrine Hydrochloride in KMBYL capsules by HPLC.[Methods] The DiamonsilTM C18(250mm?4.6mm,5 ?m)column was used.The mobile phase was mobile phase: acetonitrine-0.02 mol?L-1potassium dihydrogen phosphate(0.2% triethylamine,adjusteding pH to 2.7 with phosphoric acid)(5∶95) with a flow rate of 1.0ml?min-1.The detection wavelength was 210 nm.The column temperature was 30℃.[Results] The linear responses range was 0.2148-2.148 ?g?mL-1 of Ephedrine Hydrochloride(r=0?9999).The average recovery was 98.85% and the RSD was 2.95%.[Conclusion] The method is simple accurate reproducible.It can be used for the quality control of KMBYL Capsules.

The LEAFY (LFY) homologous gene of Dendrobium moniliforme (L.) Sw. was cloned by new primers which were designed based on the conservative region of known sequences of orchid LEAFY gene. Partial LFY homologous gene was cloned by common PCR, then we got the complete LFY homologous gene Den LFY by Tail-PCR. The complete sequence of DenLFY gene was 3 575 bp which contained three exons and two introns. Using BLAST method, comparison analysis among the exon of LFY homologous gene indicted that the DenLFY gene had high identity with orchids LFY homologous, including the related fragment of PhalLFY (84%) in Phalaenopsis hybrid cultivar, LFY homologous gene in Oncidium (90%) and in other orchid (over 80%). Using MP analysis, Dendrobium is found to be the sister to Oncidium and Phalaenopsis. Homologous analysis demonstrated that the C-terminal amino acids were highly conserved. When the exons and introns were separately considered, exons and the sequence of amino acid were good markers for the function research of DenLFY gene. The second intron can be used in authentication research of Dendrobium based on the length polymorphism between Dendrobium moniliforme and Dendrobium officinale.

Objective: To identify the spatio-temporal clustering analysis of mumps in Wenzhou City, Zhejiang Province from 2010 to 2023, so as to provide the basis for improving mumps prevention and control strategies. Methods: Data of mumps cases in Wenzhou City from 2010 to 2023 were collected from the Monitoring and Reporting Management System of Chinese Disease Prevention and Control Information System. The spatio-temporal clustering characteristics of mumps incidence were identified using spatial autocorrelation analysis and spatio-temporal scan analysis. Results: A total of 20 455 mumps cases were reported in Wenzhou City from 2010 to 2023, with an average annual incidence of 17.54/105. There were 12 919 male and 7 536 female cases, with a male-to-female ratio of 1.71∶1. The children aged 5-<10 years had the highest incidence of mumps at 135.29/105. The incidence of mumps showed a downward trend from 46.82/105 in 2010 to 3.59/105 in 2023 (P<0.05). The incidence of mumps peaked from May to July and from November to January during 2010 and 2012, the winter peak became less evident after 2013, and no seasonal trends were observed after 2020. Spatial autocorrelation analysis showed there was a positive spatial correlation of mumps of other years, with the exception of 2018 (all Moran's I >0, all P<0.05). Lucheng District, Longwan District, Ouhai District, Cangnan County and Rui'an City were high-high clustering sites. Spatio-temporal scan analysis showed that the primary clustering area was centered in Nanbaixiang Street, Ouhai District, covering 50 towns (streets), with the clustering time from April 2010 to August 2013; the secondary clustering area was centered in Zaoxi Town, Cangnan County, covering 24 towns (streets), with the clustering time from January 2010 to June 2013. Conclusions The incidence of mumps in Wenzhou City from 2010 to 2023 showed a downward trend. The urban areas, Cangnan County and Rui'an City were the clustering areas.

Objective To detect dengue virus infection by serological method and to determine the sequences of E gene of dengue virus isolated from Guangzhou in 2006.so as to clarify the possible origin of dengue fever.Methods IgM and IgG antibodies to dengue virus were detected by immunochromatographic test(ICT);NSI antigen and IgM antibody were detected by enzyme-linked immunosorbent assay(ELISA).The virus was cultured and isolated from the serum samples within 2 days using C6/36 cell lines and was identified by immuno-fluorescence assay(IFA)and RT-PCR.The E gene of isolated virus DV1-GZ42/06 was sequenced;homological analysis and phylogenetic tree analysis were performed by comparing with the reference strains and epidemic virus strains.Results The positive rates of IgM and IgG of dengue virus in patients were 89.5%(433/484)and 38.0%(184/484)by ICT,respectively.The positive rates of NS1 antigen were 92.7%(38/41)in day 1 to day 2,83.3%(70/84)in day 3 to day 5,and 10.9%(5/46)in day 6 to day 10;and the IgM detection rates were 2.4%(1/41),51.2%(43/84)and 97.8%(45/46)at the same period by ELISA.Twenty-five strains of dengue virus were isolated from 41 serum samples(6 1.O%)and were identified as type 1 dengue virus by IFA and RT-PCR.The sequencing and phylogenetic analysis of the E gene showed that the homology between the isolated Guangzhou/42/06 strain and standard strain Hawaii/45 was 94.6%.and it had a high homology with the Thailand/NI09V104,Vietnam/06.and Vietnam/07 isolates(99.0%,98.6%and 98.6%,respectively)and belonged to the same cladogram,but had low homology with the isolated strain from Guangdong before 2006.Conclusions The detection of NS1 antigen is important in the early diagnosis of dengue fever.The outbreak of dengue fever in Guangzhou in 2006 was possibly caused by the cases from neighboring countries.

This study was aimed to examine the effect of TREK-1 silencing on the function of astrocytes. Three 21-nucleotide small interfering RNA (siRNA) duplexes (siT1, siT2, siT3) targeting TREK-1 were constructed. Cy3-labeled dsRNA oligmers were used to determine the transfection efficiency in cultured astrocytes. TREK-1-specific siRNA duplexes (siT1, siT2, siT3) at the optimal concentration were transfected into cultured astrocytes, and the most efficient siRNA was identified by the method of immunocytochemical staining and Western blotting. The proliferation of astrocytes tranfected with TREK-1-targeting siRNA under hypoxia condition was measured by fluorescence-activated cell sorting (FACS). The results showed that TREK-1 was expressed in cultured astrocytes. The dsRNA oligmers targeting TREK-1 could be transfected efficiently in cultured astrocytes and down-regulate the expression of TREK-1 in astrocytes. Moreover, the down-regulation of TREK-1 in astrocytes contributed to the proliferation of astrocytes under hypoxia condition as determined by cell cycle analysis. It was concluded that siRNA is a powerful technique that can be used to knockdown the expression of TREK-1 in astrocytes, which helps further investigate the function of TREK-1 channel in astrocytes under physicological and pathological condition.

Objective To investigate the effects of numerous re-planning strategies on the anatomic and dosimetric outcomes of target volume and organs at risk (OARs) in patients with head and neck cancer receiving fractionated radiotherapy.Methods From 2015 to 2016,28 patients with head and neck cancer were enrolled in this study with Shandong Cancer Hospital,consisting of 19 patients with nasopharyngeal carcinoma, 4 patients with laryngocarcinoma, and 5 patients with carcinoma of the maxillary sinus.All of them received conventionally fractionated radiotherapy.Each patient had six weekly cone-beam CT (CBCT) scans, which were performed on the first day of every week, to obtain reference images.A virtual CT image was generated by registration of planning CT and each weekly CBCT image.The four re-planning strategies were used for the reconstruction of re-planned dose, while the initial planning was used as a reference.The weekly doses calculated using virtual CT were summed together to obtain the actual dose.The actual and initial planned doses were evaluated.The nonparametric Friedman test was used to evaluate the differences between multiple groups, and the differences between any two groups were analyzed by paired t test.Results The sizes of planning target volume, clinical target volume, and left/right parotid glands (PGs) changed significantly within the six weeks (P=0.041, 0.046, 0.024, and 0.017, respectively).For these four re-planning strategies, there were significant differences between the actual dose and the initial planned dose to the PGs (all P<0.05), with average values decreased by 5.02%, 11.17%, 12.08%, and 13.19%, respectively, compared with that in the reference strategy.Conclusions Re-planning during treatment course could ensure the sparing of OARs and allow for sufficient dose to the target volume.The higher the number of re-planning strategies, the more the actual dose is close to the initial planed dose;the efficiency of two re-planning strategies is the highest.