1.Design and development of institutional scientific research management information system
Changqin LI ; Jinghui WEI ; Dong WU
Chinese Journal of Medical Library and Information Science 2014;(11):65-67,74
After the institutional scientific research management at present was analyzed according to the literature investigation, expert consultation, comparative and comprehensive analysis, the following were advanced, including the principles, target, function module and structure module for institutional scientific research management infor-mation system , and realization of its functions using the J2 EE technology-based B/S framework .
2.Expression and significance of TLR2 and HIF-1α in pancreatic cancer
Jianjun ZHANG ; Heshui WU ; Lin WANG ; Hailong WU ; Jinghui ZHANG
Chinese Journal of Hepatobiliary Surgery 2011;17(1):26-30
Objective To investigate the expression of TLR2 and HIF-1α in pancreatic cancer and explore the relationship between TLR2 or HIF-1α protein and the clinical or pathological changes in pancreatic cancer. Methods The mRNA of TLR2 and HIF-1α in 30 cases of pancreatic cancer and its adjacent tissues were detected with real-time PCR and with immunohistochemical methods in 65 cases of pancreatic cancer and 38 cases of corresponding adjacent tissues. The relationship between TLR2 or HIF-1α and pathologic features of pancreatic cancer was analyzed. The correlation between TLR2 and HIF-1α in pancreatic cancer was also assessed. Kaplan-Meier method was used to assess the impact of TLR2 or HIF1αexpression on survival. Results The relative quantification of TLR2 mRNA and HIF-1α mRNA in pancreatic cancer tissues was 0.84±0.17 and 0.87±0.11, respectively, which was significantly higher than that in adjacent tissues 0.70±0.13 and 0.68±0.13 respectively,P<0.05. The protein expression of TLR2 and HIF-1α in pancreatic cancer tissues was 63. 10% and 70.8%, respectively, significantly higher than that in adjacent tissues (34.20% and 36.8%, respectively). There was no significant correlation between the expression of TLR2 or HIF-1α protein and the age, gender, tumor location, the degree of differentiation in patients with pancreatic cancer (P>0.05). However, there was significant correlation between the expression of TLR2 or HIF-1α protein and tumor size, lymph node metastasis, venous invasion and clinical staging. TLR2 and HIF-1α had a significant impact on survival. Conclusion TLR2 and HIF-1α overexpressed in pancreatic cancer and TLR2 signaling pathway may promote development of the pancreatic cancer with HIF-1α together.
3.Therapeutic Observation on Tuina plus Electroacupuncture for Lateral Humeral Epicondylitis
Shisheng LI ; Yaochi WU ; Junfeng ZHANG ; Jinghui ZHOU
Journal of Acupuncture and Tuina Science 2014;(5):321-325
Objective: To observe the clinical efficacy of tuina combined with electroacupuncture (EA) in treating lateral humeral epicondylitis. <br> Methods: Totally 118 patients were randomly allocated into a treatment group and a control group, 59 cases in each. The patients in the treatment group were treated with tuina combined with EA treatment, while those in the control group were treated by EA alone. Clinical efficacies of the two groups were compared after the treatment. <br> Results: The total effective rate was 93.2% in the treatment group, versus 84.7% in the control group, and the difference was statistically significant (P<0.05). <br> Conclusion: Tuina combined with EA treatment for lateral humeral epicondylitis has a significant effect.
4.Effect of apelin-13 on myocardium apoptosis in diabetic rats
Hang ZHAO ; Jinghui FENG ; Ying LU ; Xiuping WU
Chinese Journal of Geriatrics 2016;35(1):85-90
Objective To investigate the effect of exogenous apelin-13 on oxidative stress, myocardial inflammatory cytokines and apoptosis in diabetic model rats.Methods A total of 40 male Wistar rats were randomly divided into 2 groups: normal control group (NC, n=8) and experimental group (EX, n=32).Diabetes was induced by feeding with high-sugary and high-fat diet for 8 weeks and a single intraperitoneal injection of streptozotocin (STZ) (30 mg/kg).The wellestablished 28 diabetic model rats were then randomly divided into 2 subgroups: model group (DM, n =14) and apelin-13 administration group (AP, n =14).The rats in AP group were given intraperitoneal administration of apelin-13 at a single dose of 0.1 μtmol · kg-1 · d-1 for 10 weeks, while NC group and DM group were given 0.9% NaCl in the same way.All rats were sacrificed at the end of the week 10.Blood hydrogen peroxide (H2O2), nitric oxide (NO) and malonaldehyde (MDA) were measured by colorimetry, and superoxide dismutase (SOD) was measured by enzyme-linked immunosorbent assay (ELISA).The expressions of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in rat myocardium were detected by immunohistochemistry.Masson staining was used to observe the myocardial fibrosis in rats of different groups.Cardiomyocyte apoptosis was determined by TUNEL.Results (1) Compared with NC groups, DM group showed that MDA, H2O2 and NO were significantly increased, while SOD was significantly decreased (F=22.400, 6.230, 4.267 and 8.901, all P<0.0167).There was a statistically significant difference in the levels of MDA, H2O2,SOD between DM group and AP group.(2) The expression of TNF-α and IL-6 was significantly higher inDMgroup than inNCgroup (0.0599±0.0208 vs.0.0076±0.0031, F=35.139;0.0503±0.0243 vs.0.0071± 0.0024, F=15.946, both P<0.0167).After 10 weeks of apelin-13 administration, the levels of inflammatory cytokines were significantly decreased in AP group than in DM group.(3) The myocardial apoptosis and fibrosis were significantly increased in DM group than in NCgroup [0.0293±0.0061 vs.0.0030-t-0.0013 and 0.0708±0.0420 vs.0.0013±0.0003, F=84.930 and 19.420, both P<0.0167].The myocardial apoptosis and fibrosis were significantly decreased in AP group than in DM group (both P<0.0167).Conclusions To some extent, apelin13 reduces the levels of oxidative stress and myocardial inflammation reaction in type 2 diabetes.Moreover, it may play a vital role in the improvement of myocardial apoptosis and fibrosis.
5.The development and application of stabilizers for ELISA test kit
Xiangli WU ; Lei SHI ; Shuo CUI ; Jinghui YAN
International Journal of Laboratory Medicine 2014;(18):2527-2528
Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer (mass fraction :bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ) and a kind of enzyme la-beled antibody stabilizer (mass fraction :Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ) were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .
6.PCR-RFLP in gene diagnosis of spinal muscular atrophy
Zhiguo WU ; Bo XIAO ; Xiaosu YANG ; Lifang ZHANG ; Jinghui LIANG
Chinese Journal of Neurology 1999;0(06):-
Objective To study the value of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in gene diagnosis on spinal muscular atrophy (SMA).Methods PCR-RFLP method was used to detect the homozygous deletion of the exon 7 or exon 8 of SMN gene in 20 SMA patients of Type Ⅰ,Ⅱ,Ⅲ and 15 normal individuals.Results Homozygous deletion of exon 7 and exon 8 of the SMN gene were all identified 7/7 in SMA TypeⅠpatients, and 5/5 and 4/5 respectively in SMA Type Ⅱ patients, but only 1/8 of SMA Type Ⅲ patients, and no homozygous deletion was found in the normal controls.Conclusions PCR-RFLP might be recommended as an effective diagnosis for spinal muscular atrophy Type Ⅰand Ⅱ patients, whereas the method might not be as useful in Type Ⅲ as in Type Ⅰand Ⅱ for the gene diagnosis.
7.Expression and clinical significance of Pin1 and cyclin D1 in pancreatic carcinoma
Meiju LIN ; Heshui WU ; Jinghui ZHANG ; Lei ZHANG ; Jianbo XU
Chinese Journal of General Surgery 1994;0(05):-
Objective To investigate the expression of Pin1 and cyclin D1 in human pancreatic carcinoma and to discuss its role in oncogenesis of pancreatic cancer.Methods The mRNA expression of Pin1 and cyclinD1 in pancreatic tumor tissues and corresponding adjacent nontumor tissues 27 patients was detected by the real-time quantitative reverse transcription-polymerase chain reaction(RQ-RT-PCR).The results of RQ-RT-PCR were analyzed by one-way ANOVA and Fisher's exact probabilities test.Results Cyclin D1 and Pin1 were overexpressed at mRNA level in pancreatic carcinoma tissues compared with their adjacent nontumor tissues.Cyclin D1 overexpression were found in 14 of 20 pancreatic carcinoma tissue specimens and Pin1 overexpression in 13 of 20 carcinoma tissue specimens.The expression of cyclin D1 and Pin1 in pancreatic cystadenoma tissues was not different than that of corresponding adjacent nontumor pancreatic tissue.Pin1 overexpression positively correlated with an increase in cyclin D1 levels as shown by Fisher's exact probabilities test.However,Pin1 and cyclin D1 expression was not correlated with clinical stage and pathological parameters.Conclusions The overexpression of Pin1 in pancreatic carcinoma tissues could promote cyclin D1 expression,which might be a critical event in oncogenesis of pancreatic carcinoma.Pin1 may play a key role in pancreatic carcinoma.
8.The protection of the hepatic ischemic preconditioning is concerned with the NO/ET-1 system
Ping LU ; Daoda CHEN ; Yuan TIAN ; Jinghui ZHANG ; Yihu WU
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To study the relationship between the disturbance of nitric oxide/endothelin-1(NO/ET-1) and hepatic ischemia/reperfusion(I/R) injury as well as the regulation of NO/ET-1 system by hepatic ischemic preconditioning(IPC). METHODS: The changes of NO/ET-1 system and their relationship with hepatic I/R injury were compared between I/R group and IPC+I/R group in a rat hepatic I/R model. Two hours after reperfusion, the liver tissues were detected by RT-PCR to see whether there was inducible nitric oxide synthase (iNOS) mRNA expression. RESULTS:In the acute phase of hepatic reperfusion, the ratio of NO/ET-1 was reduced, which was due to a significant reduction of NO - 2/NO - 3 (the metabolic product of NO) and significant elevation of ET-1 in the blood plasma. The content of ALT, AST, LDH and TNF-? in blood plasma, and of MDA in liver tissue were increased but ATP in liver tissue was reduced, the hepatic damage was deteriorated. The protection of the hepatic IPC was concerned with the elevation of the ratio of NO/ET-1 caused by the elevation of NO - 2/NO - 3, and reduction of ET-1 as well. There was no iNOS mRNA detected in the liver tissues.CONCLUSION: Hepatic I/R injury is related to the disturbance of NO/ET-1. The protection of the hepatic IPC in the acute phase might be conducted by its regulation of NO/ET-1 system. The cNOS rather than the iNOS generated the NO in this situation.
9.Expression of serum periostin in children with dilated cardiomyopathy and its clinical significance
Lan WU ; Jinghui SUN ; Chunyan ZHANG ; Zhaoxia WANG
Chinese Journal of Applied Clinical Pediatrics 2014;29(22):1734-1736
Objective To investigate the correlation of serum periostin and severe degree of dilated cardiomyopathy(DCM) in children.Methods Thirty-two children with DCM from Jan.2009 to Jun.2013 in First Hospital Affiliated to Jilin University were selected as DCM group.Then these children were divided into 0-2 score group (n =6),3-6 score group (n =7),7-9 score group (n =11),and 10-12 score group (n =8) according to Ross standardization.Twenty healthy children in the same period were selected as control group.Meanwhile,the levels of serum periostin were detected by using enzyme-linked immunosorbent assay,the levels of serum creatine kinase-MB(CK-MB)were detected by adopting immunosuppressive method,while left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD) were observed by using uhrasonography.The data in various groups were compared and the relationship between serum periostin and ROSS,LVEF was analyzed by performing linear regression analysis.Results 1.Compared with control group,the level of serum periostin in DCM group was significantly increased,and the difference was significant (P =0.00).The serum periostin level increased with the increase of ROSS score increase,and there were significant differences among the different ROSS score groups (all P < 0.05).2.Compared with control group,the level of serum CK-MB in DCM group was significantly increased,the difference was significant (P =0.00) ;while the serum CK-MB levels had no significant difference among the different ROSS score groups(all P>0.05).3.Compared with control group,the level of LVEF in DCM group was significantly decreased,the differences was significantly(P =0.00) ; and the LVEF level decreased with the ROSS score increase,there were significant differences among the different ROSS score groups(all P < 0.05).4.Compared with control group,the level of LVEDD in DCM group was significantly enlarged,and the difference was significant(P =0.00) ; but the LVEDD had no significant differences among the different ROSS score groups (all P > 0.05).5.Significantly positive correlation was found between the level of serum periostin and ROSS score (r =0.742,P < 0.001).There was no obvious correlation between the level of serum periostin and CK-MB(r =0.247,P > 0.05).There was negative correlation between the level of serum periostin and LVEF (r =-0.424,P < 0.01).Conclusions The level of serum periostin is positively correlated with ROSS score and has negative correlation with LVEF.It may provide a useful index in the assessment of symptomatic state in children with dilated cardiomyopathy.
10.Effect of Bailing capsules on periostin expression in myocardium tissue of viral myocarditis mice with myocardium fibrosis
Lan WU ; Zhaoxia WANG ; Man GAO ; Chunyan ZHANG ; Jinghui SUN
Chinese Journal of Applied Clinical Pediatrics 2015;30(1):64-67
Objective To explore the preventive effect of Bailing capsules(BL) on myocardial fibrosis in the viral myocarditis(VMC) mice models and to clarify the possible mechanism.Methods The models of VMC were established by injecting Coxsackie virus B3 (CVB3) solution into 50 BALA/C mice.The 31 mice were divided into model group(n =11),large dose BL group(n =10)and small dose BL group (n =10).At the same time the control group (n =10) was established.The mice in model group and control group were fed with water 1 mL/d,the mice in large dose BL group were fed with BL at a dose of 7.5 g/(kg · d),and the mice in small dose BL group were fed with BL at a dose of 2.5 g/(kg · d).After 60 d,the levels of angiotensin Ⅱ (Ang Ⅱ) in blood serum of mice in various groups were detected by using ELISA.Myocardium tissue of mice was stained by Masson and collagen volume fraction(CVF)was accounted.At the same time,the content of periostin mRNA and transforming growth factor β1 (TGF-[β1) mRNA in myocardium tissue of mice were detected by adopting reverse transcription(RT)-PCR.Results Compared with the control group,the levels of serum Ang Ⅱ,CVF,TGF-β1 mRNA and periostin mRNA were increased (all P < 0.01) in the model group.Compared with the model group,the levels of serum Ang Ⅱ,CVF and TGF-β1 mRNA were obviously decreased in large dose BL group and small dose BL group (all P < 0.05).Compared with the model group,the level of periostin mRNA was obviously decreased in large dose BL group (P < 0.05),but the level of periostin mRNA in small dose BL group was not decreased obviously (P > 0.05).Conclusions BL can offer some protection to myocardial fibrosis in VMC mice.The possible mechanism may be performed through inhibiting the expression of serum Ang Ⅱ and TGF-β1 in myocardium tissue to reduce the expression of periostin.