Objective To study influence of travoprost eye drops in treatment of after anti-glaucoma surgery and its effects on visual function, the tear film stability and inflammatory index. Methods 90 patients of anti glaucoma who received therapy from August 2014 to August 2015 in the first hospital of Ninghai County Zhejiang Province were selected as research objects. The control group was treated with timolol maleate, while the observation group was treated with travoprost eye drops, then the visual acuity, intraocular pressure, tear film stability (rupture time, schirmer), inflammation (flashlight, cell count), visual field defect percentage range, curative effect after treatment were compared. Results After treatment, the vision in observation group (0.95±0.26)D was better than the control group (0.76±0.21)D, intraocular pressure in observation group (11.29±3.23) mmHg was less than the control group (13.89±3.72)mmHg, the difference was statistically significant (P<0.05), rupture time in observation group (12.93±1.90)s was higher than the control group (10.36±1.80)s, schirmer in observation group (13.01±1.60)mm was higher than the control group (11.10±1.02)mm, the difference was statistically significant (P<0.05), TNF-α, IL-6 in observation group was less than the control group, the difference was statistically significant (P<0.05), field of visual field defect in observation group (38.96±10.21)% was less than the control group (47.37±11.35)%, the difference was statistically significant (P<0.05), the total effective rate of observation group 95.56%(43/45) was statistically higher than that in the control group 77.78%(35/45), the difference was statistically significant (P<0.05). Conclusion Travoprost eye drops can improve glaucoma postoperative visual function, tear film stability, reduce inflammation.

Objective To observe the protective effect of alcholic extractive of Cornu Cervi against the myocardial damage of acute myocardial infarction model rats. Methods The myocardial ischemia rats model was made by ligaturing their coronary attery, and the indexes including electrocardiogram, the area of acute myocardial infarction and ET of acute myocardial infarction model rats were observed after the acute period of myocardial damage. Results The alcoholic extractive of Cornu Cervi could obviously improve the degree of myocardial ischemia, reduce the area of myocardial ischemia, and lessen the level of ET. Conclusion The alcoholic extractive of Cornu Cervi could protect the myocardial against the damage of acute myocardial infarction model rats in some degree. The mechanism may be related to the reduction of value of ET of myocardial damage.

Objective To investigate the effect of Huangqikeli on immune function of mice. Methods 30 mice were randomly divided into 3 groups. The mice in A and B group were administrated with distilled water,C group were administrated with Huangqikeli. On the tenth day,all groups except A group were injected with cyclophosphamide to induce the model. The effects on the phagocytic function of Huangqikeli to macrophage in abdominal cavity of mice,delayed hypersensitivity induced by DNCB,and production of hemolysin antibody in mice that CRBC sensitized were observed. Result Compared with immuno-suppressive models given distilled water (B group),phagocytic function of macrophage in abdominal cavity of mice and delay hypersensitivity leaded by DNCB were enhanced (P

Objective To investigate the influence of Yudan Rongxin Keli on immunoregulation in mice. Methods The mice were randomly divided into the normal group, cyclophosphamide (Cy) model group, Yudan Rongxin Pill group and Yudan Rongxin Keli group. The effects of Yudan Rongxin Keli on the phagocytosis of macrophage in mouse abdomen, delayed-type hypersensitivity (DTH) induced by dinitrochlorobenzene (NDCB), and generation of hemolysin antibody induced by chicken red blood cell (CRBC) were observed respectively. Result Compare with the Cy model group, the phagocytosis of macrophage in Yudan Rongxin Keli group was promoted (P

Objective To investigate the effect of cytochrome P_ 450 2A6 (CYP2A6) genetic polymorphisms on serum concentration of sodium valproate. Methods A total of 98 epileptic patients receiving sodium valproate after a period of more than 5 half-time were recruited. The genotypes of CYP2A6 of the patients were detected by nested-primer polymerase chain reaction (PCR) to examine the alleles CYP2A6*1 and CYP2A6*4. Fluorescence polarization immunoassay (FPIA) was used to measure the serum concentration of sodium valproate. Results Of the 98 cases, 73 (74.5%) were wild genotypes, 24 (24.5%) were CYP2A6*1/*4 genotypes and 1(1.0%) was CYP2A6*4/*4 genotype. According to the genotypes of CYP2A6 the patients were divided into two groups,one was group A (CYP2A6*1/*1) and the other was group B (CYP2A6*1/*4 or *4/*4). The mean value of the serum concentration of sodium valproate of the patients in group A(4.1393?0.2793) was higher than that in group B(3.3486?0.3919) with a statistical significance (P

BACKGROUND: Electro-acupuncture therapy shows good central and peripheral analgesic effects. Several studies have shown that transforming growth factor β (TGF-β) plays an important role in the pathogenesis of knee osteoarthritis. OBJECTIVE: To investigate the mechanism underlying electro-acupuncture treatment of knee osteoarthritis. METHODS: A total of 80 healthy male 3-month-old Sprague Dawley rats were randomly and evenly divided into normal, model, electro-acupuncture and drug groups. Rat models of knee osteoarthris were estbalished by ligating the femoral veins and forcing rats to do activies. At 1 month after knee osteoarthris induction, the electro-acupuncture group rats received electro-acupuncture therapy at two acupoints Neixiyan (EX-LE4) and Dubi (ST 35) with a depth of 0.1 cun (pulse 2 Hz, 20 minutes, once a day). The drug group rats were intraarticularly administerd sodium hyaluronate (0.1 mL/administration, once a week). After 2-week treatment, synovial tissue of the knee joint was harvested to determine the exprssion of TGF-β1, TGF-β1 receptor Ⅰand TGF-β1 receptor Ⅱ. RESULTS AND CONCLUSION: TGF-β1 expression in synovial tissue of the knee joint was significantly increased after knee osteoarthris (P < 0.05), but after electro-accupuncture therepy or sodium hyaluronate treatment, TGF-β1 expression was significantly decreased (P < 0.05), moreover, TGF-β1 receptor Ⅰ, Ⅱ expression was signficantly decreased (P < 0.05). These findings suggest that electro-acupuncture for treatment of knee osteoartheis improves the symptoms of osteoarthris by downregulating TGF-β1 expression, and reduction in TGF-β1 receptor Ⅰ, Ⅱ expression promotes the recovery of knee osteoarthris.

Objective To explore the levels and clinical significance of anti-beta 2-glycoprotein 1 antibodies (β_2GP1), matrix metalloproteinase 9 (M M P-9) in the plasma of children with Kawasaki diseases (KD). Methods Serum level of anti-β_2GP1 antibody and MMP-9 was measured in 47 children with KD by ELISA, and the data was analyzed using SPSS11.5 software. Thirty age matched children with infectious diseases(sepsis or pneumonia), exclusive of heart, liver, kidney, blood diseases and autoimmune diseases such as rheumatoid were chosen in the fever control group. Results Coronary artery lesions (CAL)were found in 17 children of KD group (17/47) by Doppler ultrasound examination. Significant differences (P < 0.05) of serum level of anti-β_2GP1 antibody was showed between KD group ((7.46 ± 2.13) U/ml)and the control group ((4.38 ± 0.43) U/ml) ; serum level of MMP-9 was (886.62 ± 92.72) ng/ml and (460.06 ± 179.59) ng/ml in KD group and the control group respectively, with significant difference between the two groups(P < 0.05). In KD group, levels of anti-β_2GP1 were (8.83 ± 0.89) U/ml among children with CAL and (6.18 ± 1.42) U/ml among children without CAL, serum level of MMP-9 was (948.62 ± 81.76) ng/ml and (872.00 ± 34.74) ug/ml respectively, with significant differences(beth P < 0.05). In children with KD, the serum levels of anti-β2GP1 antibody and MMP-9 were significantly correlated (correlation coefficient r = 0.665). Conclusions Serum levels of anti-β_2GP1 antibodies and MMP-9 increased in the acute phase of KD, and were significantly higher in those KD children with CAL.Anti-β_2GP1 antibodies and MMP-9 may play a role in the pathogenesis of KD, and can be used as an important serological indicator of KD with CAL.

Objective: To confirm the pathogenesis and diagnosis of pediatric Fisher's syndr ome.Methods: Clinical manifestations and Cerebrospinal fluid (CSF) were compared and studied in 22 patients diagnosed as Fisher's syndrome.Results:Twenty- two cases of Fisher's syndrome both had the characteristics of ophthalmoplegia and cerebella ataxia,and had different degrees of incomplete fl accid paralysis in the ar ms and legs. In about half of 22 cases, the CSF showed divergence phenomenon of the protein and cell. Conclusion: Fisher's syndrome is a specific or variant type of AIP involving the cranial nerves, in which the cerebellar ataxia is taken as a main symptom and sign .

The human La protein is recently identified as a host factor potentially involved in the post-transcriptional regulation of hepatitis B virus (HBV) RNA. The La binding site is mapped to a predicted stem-loop structure within a region shared by all HBV RNAs, and it is known that human La protein protected HBV RNA against Rnase-mediated degradation. HLa mutants lost the ability of binding and protecting HBV RNA, which make it easy for HBV RNA to degrade and the virus replication to terminate. This review summarizes the latest investigation about the interaction of La protein,HBV RNA and Nuclear RNases, and discusses the possible mechanisms of HBV RNA degradation, the effect of La protein and its mutants on the translation initiation of HBV RNA, and the replication of the virus.

OBJECTIVE:To evaluate the human body pharmacokinetics and bioequiavailability of two kinds of oral single dose of hydrochloric itraconazole capsules.METHODS:A randomized,crossover study of20healthy volunteers receiving sin-gle oral dose of200mg itraconazole was conducted with in vivo blood concentrations determined by HPLC-fluorescence de-tection.RESULTS:The pharmacokinetic parameters for the testing itraconazole and the reference itraconazole were as fol-lows,t 1/2 were(29.3?5.62)h and(29.3?5.81)h,respectively;C max were(81.4?60.0)?g/L and(77.8?45.2)?g/L,respec-tively;t max were(3.9?0.70)h and(4.2?0.70)h,respectively;AUC 0～72 were(1199.4?649.6)(?g?h)/L and(1174.3?701.9)(?g?h)/L,respectively;AUC 0～∞ were(1414.0?815.2)(?g?h)/L and(1386.1?735.8)(?g?h)/L,respectively.there were no significant differences in main pharmacokinetics parameters between2preparations,except in t max from analysis of variance and one-side&two-sides t-tests.The relative bioavailability of trial itraconazole capsule was(105.3?23.4)%.CONCLUSION:These two kinds of itraconazole capsules are bioequivalent.