Cytokeratin 8 ( CK8 ) belongs to the keratin family of intermediate filament cytoskeletal proteins. A lot of researches have demonstrated that abnormal expression or post-translational modifications of CK8 may affect its activity and consequently affect the development and progression of tumors. Therefore, investigating the role of CK8 in tumorigenesis may contribute to the prevention and treatment of tumors.

Objective To study the effect of early application of naloxone on serum high sensitivity C-reactive protein(hs-CRP) and S100B protein in neonates with hypoxic-ischemic encephalopathy.Methods According to the digital table,86 neonates with hypoxic-ischemic encephalopathy were randomly divided into the control group (n =43 cases) and the observation group (n =43 cases).The control group was treated by conventional therapy,while the observation group was treated by conventional therapy plus naloxone.They were treated for 7-10 days.The clinical effect was evaluated.Serum hs-CRP and S100B protein were detected before and after treatment.Results The total effective rate of the control group was 86.0％,which was significantly lower than 97.7％ of the observation group (x2 =5.78,P ＜ 0.05).The sense of recovery,reflecting the recovery and muscle tension recovery time of the observation group were less than those in the control group (t =5.017 2,3.991 6,2.904 8,all P ＜ 0.05).After treatment,the sertum hs-CRP and S100B protein in the two groups were significantly decreased (t =6.776 2,10.903 8,7.772 8,11.092 6,all P ＜ 0.01),compared with the control group,the decrease of the observation group was more significant (t =3.368 2,3.092 8,all P ＜ 0.05).Conclusion Early application of naloxone in neonates with hypoxic-ischemic encephalopathy can decrease serum hs-CRP and S100B protein.

Objective To evaluate the clinical effect of transforaminal decompression posterior interbody fusion internal fixation in the treatment of upper protrusion of lumbar intervertebral disc .Methods 11 patients with upper protrusion of lumbar interverte-bral disc were given transforaminal decompression posterior interbody fusion internal fixation .The operation time and bleeding a-mount were recorded ,and the clinical effects were evaluated .Results The operation time and bleeding amount during operation were (132 .0 ± 37 .0)min and (320 .1 ± 56 .8)mL ,respectively .The lumbar function scoring of Japanese Orthopedic Association (JOA) after operation was (22 .9 ± 2 .3) points ,which was significantly higher than (11 .2 ± 3 .1) points before operation ;The postoperative recovery excellent rate was 90 .9% .All of the patients successfully finished posterior interbody fusion .Conclusion Transforaminal decompression posterior interbody fusion internal fixation in the treatment of upper protrusion of lumbar interverte-bral disc has the advantages of sufficient exposure ,safe operation and significant therapeutic effects .

The changes of zine content in serum and feces observed after intragastric administration of a certain dose of dithizone to the pregnant rats in their third trimester for one week, and the developing status of brain of their pups were studied. The result showed that the total fecal exerction of zine during 24 hours increased and serum zine content decreased significantly aftcr continual administration of dithizone for one week in maternal rats. It suggested that dithizone may be used for making animal model of zine deficiency. The body and brain weights, brain protein content and RNA/DNA ratio of the newborn delivered by the zine-deficiency maternal rats decrcased significantly. This indicates that the maternal zine dificiency in third trimester gives side effects on the development of their fctal brain.

t affect the near stereopsis, showing that increasing vertical fusion range shouled be a compensation for maintaining the binocular vision caused by vertical deviation.

Objective To investigate the relation of resistant index and function of transposited ovaries following radical hysterectomy in young women with early stage of cervical cancer.Methods A total of 22 young women(aged from 26 to 40 years old)with early stage of cervical cancer(FIGO Ⅰ a or Ⅱ a)were studied from Aug.1997 to Sep.2004 in the Affiliated Hospital of Inner Mongolia Medical Collage.Ovarian transposition was performed at the time of radical hysterectomy and pelvic lymphadenectomy in 12 cases who received postoperative radiotherapy;the rest 10 cases did not receive postoperative radiotherapy.Ovarian function was evaluated by serum FSH,LH,E_2 and B-ultrasonography in all of the cases.Results There was a statistically significant difference between the radiotherapy group and the non-radiotherapy group in serum E_2,FSH,LH levels and RI at the 24th month postoperatively.RI had a negative correlation with E_2 and positive correlation with FSH.Conclusion Ovarian transplantation can preserve the endocrine function of ovary for young women with cervical cancer.RI has a negative correlation with E_2 and positive correlation with FSH.RI is the important index of supervising ovarian function.Postoperative radiotherapy can influence RI and ovarian function.

Objective To investigate the relationship between the ultrastructural features combined with the expression of connexin ( Cx43 ) protein in uterine junction zone and pathogenesis of adenomyosis.Methods From Nov. 2008 to Nov. 2009, 30 patients with adenomyosis (including 14 cases with proliferative endometrium and 16 cases with secretory endometrium) as study group matched with 30 women with cervical intraepithelial neoplasia (GIN) Ⅲ treated by hysterectomy as control group were enrolled in this study in Affiliated Hospital to Inner Mongolia Medical College. The expression of Cx43 in uterine junction zone of patients with adenomyosis was detected by immunohistochemisty staining. The ultrastucture of eutopic endometrium, uterine junction zone and outer 1/3 myometrium in both groups without history of dilatation and curettage, C-section and uterine surgery were observed by using transmission electron microscopy. Results ( 1 )The expression of Cx43 in proliferative and secretroy uterine junction zone were 0.133 ±0.018 and 0.137 ± 0.021 in study group and 0.154 ±0.016 and 0.141 ±0.018 in control group,which reached statistical difference (P ＜0.05). However, it didn't show significant expression of Cx43 between proliferative and secretory uterine junction zone in study or control group ( P ＞ 0.05 ). The expression of Cx43 in proliferative and secretory of eutopic endometrium of 0.067 ± 0.017 and 0.062 ± 0142 in study group were significantly lower than 0.094 ±0.005 and 0.080 ±0.005 in control group. It didn't show statistical difference of Cx43 expression between proliferative and secretroy eutopic endometrium in both group. The expression Cx43 in outer myometrium of proliferative phase were 0.184 ± 0.022 in study group and 0.188 ± 0.028 in control group, which did not show significant difference (P ＞0.05). It also did not exhibit statistical difference of Cx43 expression in outer myometrium of secretory phase (0.178 ± 0.022,0.191 ± 0.025,P ＞0.05). (2) Morphological changes: the area of uterine smooth muscle cells of the uterine junction zone of (24. 3 ± 1.6) μm2 in study group were significantly increased than (21.8 ±2.0)μm2 in control group (P ＜ 0.01 ). The length of the cell membrane dense plaques of (1.07 ± 0. 17 )μm in study group was significantly increased than (0.71 ±0.07) μm in control group (P ＜0.01 ). The myocytes exhibited cellular hypertrophy and disordered arrangement and fewer caveolae. There was cylindrical and dentate, chromatin margination, more heterochromatin in which muscle cells of nuclear surface of the uterine junction zone. Less cytoplasmic myofilaments and more intermediate filaments. Mitochondria were increased,the volume increased significantly vacuolization. The rough endoplasmic reticulum and Golgi apparatus were more prominent. Otherewise, mast cells and fibroblasts were close and glandular epithelial cells showed desmosome connection which villus thickening and dense. All features were more prominent at the junctional zone. Conclusions The down-regulation of Cx43 expression and ultrastructure changes in the junction zone might play an important role in pathogenesis of adenomyosis.

Objective To observe the clinical treatment effects of Qingkailing injection on Alzheimer's disease.Methods 84 patients with AD were randomly divided into a control group and a treatment group (n=42 cases,per group). The patients in both groups orally took 5 mg of donepezil hydrochloride every night. The Qingkailing injection of 60 ml was administrated by intravenous drip in addition to oral uptake of donepezil hydrochloride to the treatment group. The total treatment period of both groups was 20 weeks. The MMSE, ADL score, HS-CRP, IL-6, MDA and ox-LDL content and the clinical treatment results and differences in the two groups before and after the treatment were observed.Results After treatment, the serum HS-CRP (4.18 ± 0.38 mg/Lvs. 5.78 ± 0.53 mg/L,t=15.900), IL-6 (34.28 ± 4.33?g/L vs.44.75 ± 4.99?g/L,t=10.270), MDA (6.04 ± 0.98?mol/L vs.7.43 ± 0.69?mol/L,t=7.516), ox-LDL (62.87 ± 7.65?g/L vs.102.56 ± 9.81?g/L, t=26.677) content of the treatment group were significantly decreased than the control group (P<0.01). The total effective rate between the treatment group and the control group had significance difference (83.3%vs.  61.9%;χ2=4.850,P=0.028). The MMSE score in both groups after the treatment were higher than those in the same group(t value were 8.036, 7.475, respectively,P<0.01), ADL scores in both groups after treatment were lower than those before treatment(t value were 7.818, 5.960, respectively,P<0.01), but no significance difference between the two groups(t value were 1.633, 1.710,respectively,P value were 0.106, 0.091, respectively).Conclusion The treatment effect of Qingkailing injection on Alzheimer's disease was sound.

Objective To explore the clinical effect of Chinese medicine enema combined with massage of Hui medicine for treatment of patients with ulcerative colitis (UC).Methods A prospective randomized controlled trial (RCT) was conducted, 390 patients with UC admitted to Department of Traditional Chinese Medicine (TCM) of Ningxia Hui Autonomous Region People's Hospital from January 2013 to December 2014 were enrolled, and they were divided by using random envelope method into the western medicine control group (88 cases), simple TCM enema group (102 cases), simple massage of Hui medicine group (94 cases) and enema combined with massage group (106 cases). The patients in western medicine control group took sulfasalazine orally, once 0.75 g, thrice a day. The simple TCM enema group adopted the self prepared Shexue Fuyang decoction (ingredients: carbonized sanguisorba root 15-20 g, bletilla striata 15 g, patrinia 15-30 g, sculellaria barbata 15-30 g, Chinese goldthread 15 g, radix glycyrrhizae preparata 15 g, Fry terminaliachebuia 10 g, yam 15-30 g, india madder root 15 g) retention enema once a day. The following principles should be followed: firstly, the dose of drug should be large enough (250 mL), secondly the speed of enema should be relatively slow and it finished in 15-20 minutes, thirdly the temperature of decoction should be kept at moderate degree about 39-41 ℃ (a warm bag being around the enema tube), and lastly, the patient's posture should be changed (lying left-side for 20 minutes, lying at chest-abdomen position for 20 minutes, lying right-side for 20 minutes). In massage of Hui medicine group, based on the location and size, depth and seriousness of the lesion, the doctor would selectively use palm or index finger to carry out massage manipulations of pressing, fractioning, rubbing or grasping for different disease situations of patients, once 30 minutes pre day. The patients in enema combined massage group were treated with the same methods as above, and the therapeutic course of the four groups was 15 days; at the end of experiment,the therapeutic effects and the improvement of main symptoms in four groups were observed.Results The clinical total effective rates of Chinese medicine enema group, massage of Hui medicine group, enema combined massage group were obviously higher than the rate of western medicine control group [78.4% (80/102), 85.1% (80/94), 93.4% (99/106) vs. 62.5% (55/88), allP < 0.05]; the symptom integrals, such as abdominal pain, diarrhea, tenesmus, purulent blood stool, fever, etc. were significantly decreased after treatment in the TCM enema, massage and combined treatment groups, and the integrals of the three treatment groups were obviously lower than those in the western medicine control group (abdominal pain: 0.45±0.75, 0.56±0.57, 0.35±0.96 vs. 1.13±0.86, diarrhea: 0.76±0.91, 0.78±0.69, 0.55±0.81 vs. 1.43±0.34, tenesmus: 0.52±0.37, 0.44±0.23, 0.21±0.13 vs. 0.72±0.98, purulent blood stool: 0.43±0.34, 0.50±0.24, 0.52±0.76 vs. 0.68±0.44, fever: 0.32±0.56, 0.35±0.65, 0.26±0.45 vs. 0.49±0.77, allP < 0.05).Conclusion The therapeutic effects of Shexue Fuyang decoction enema combined with massage of Hui medicine for treatment of patients with UC are better than those of each of the following treatment alone: western medicine, Chinese medicine enema or medical massage treatment.

18F fluorodeoxyglucose, as a kind of tracer, has its limitations in positron emission tomography/computed tomography (PET/CT). 18F-fluorothymidine (18F-FLT) is a kind of new tracer. The value of 18F-FLT in diagnosis of tumor, tumor staging and assessment of therapeutic effects was reviewed in this article.