Objective To investigate the effect of health education on the family care for elderly patients.Methods Health education were done during family care for 65 elderly patients.A comparison was done between pre-intervention and post-intervention effect in terms of mastery of disease knowledge,health behavior,curative compliance and emotion management.Result The effect after intervention was significantly better than that before intervention (χ2=28.770,P<0.01).Conclusion During family care for elderly patients,health education is effective for the improvements of mastery of disease knowledge,health behavior,curative compliance,emotion management and ultimately the quality of life.

Objective To expore the mechanism of low-dose interfone-γ(IFN-γ) influences on differentiation of oligodendrocyte precursor cell. Methods The cerebral cortex samples were obtained from one day old SD rats to form mixed single cell suspensions. After culturing in full medium for 7 to 10 days, succession and differential velocity adherent technique were performed to acquire oligodendrocyte precursor cell and cultured in serum-free medium. IFN-γ, AG490 and Fludarabine were added during the culture of oligodendrocyte precursor cell and reverse transcription-polymerase chain reaction, Western blot and flow cytometry were performed to evaluate the expression of intracellular P27kip1 and its influence on the differentiation of oligodendrocyte precursor cell. Results (1)The expression of P27kip1 mRNA and protein was lower in IFN-γ group than in control group (t=85. 535, P<0. 05;t= 12. 481, P<0. 05), while the expression of P27kip1 mRNA and protein in IFN-γ+AG490 group and IFN-γ+Fludarabine group were both higher than those in IFN-γ group (P<0. 05). (2) The phosphorylation levels of JAK2/STAT1 in INF-γ group were higher than that in the other three groups (P<0. 05). (3) The percentage of myelin basic protein positive cells was (68. 42 ± 2. 53)% in IFN-γ group, lower than that in control group [(88.21 ± 1.97)%](t=10.682, P < 0.05). Myelin basic protein positive cells in IFN-γ + AG490 group were (57. 63 ±2. 75) %, lower than those in the IFN-γ group. The same figure in IFN-γ+Fludarabine group were (79. 53±4. 15)% , higher than those in IFN-γ group (t = 3.957, P<0.05). Conclusions Low-dose IFN-γ can regulate the expression of intracellular P27kip1 through JAK2/STAT1 signal transduction pathway and Fludarabine may participate in this process and improve the differentiation and maturation of oligodendrocyte precursor cell.

Objective To compare the field-matching techniques and dosimetric characteristics in the target area of testicular seminoma including the abdominal and pelvic cavities between direct aperture optimization intensity modulated radiation therapy (DAO-IMRT) and 3D conformal radiation therapy (3D-CRT),and to analyse the advantages and disadvantages of DAO-IMRT.Methods DAO-IMRT and 3D-CRT plans were designed with Pinnacle treatment planning system for 7 testicular seminoma patients,and the characteristics of both the irradiation methods were analyzed and evaluated by means of the parameters like dose homogeneity indexes,dose volume histograms and etc.Results DAO-IMRT had the hot and cold volumes in the target areas both significantly smaller than those by 3D-CRT,while the conformity index and homogeneity index superior to those by 3D-CRT.The mean doses (Dmean) by DAO-IMRT of the organs at risk (OAR) except the left femur head were all lower than those by 3D-CRT,aud the V15 values of liver,double kidney,small intestine and rectum by DAO-IMRT were statistically lower than those by 3D-CRT (P＜0.01).3D-CRT had the V5 values of OAR all lower than those by DAO-IMRT,in which the differences of double kidney,small intestine,bladder,left femur head and normal tissues were significant statistically (P＜0.05).The monitor units by DAO-IMRT roughly doubled when compared with those by 3D-CRT,and the difference was also significant (P＜0.01).Conclusion DAO-IMRT with easy operation and high reliability can avoid the connection of fields in the target area of testicular seminoma,and is superior to 3D-CRT in dosimetry.

0.05). CONCLUSION: The effects of 10 mg?kg -1 m oxonidine on reduced blood pressure and heart rate in SHR is equal to the effect s of 1 mg?kg -1 clonidine after once large dose oral administration. T here is no significant difference between the moxonidine and clonidine of the sa me dose in reducing blood pressure after repeatedly small dose oral administrati on in SHR.

OBJECTIVE To investigate genes associated with the drug-resistance of ?-lactam antibiotics in Pseudomonas aeruginosa(PAE) isolated from clinical patients. METHODS ?-Lactamase genes including TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,FOX,MOX and oprD2 were detected by PCR amplification in 33 PAE isolates. RESULTS TEM,SHV,GES,CARB and VIM genes were positive in 100%,6.1%,6.1%,9.1% and 12.1% of 33 isolates,respectively.The deletion of oprD2 gene was found in 22 isolates.Other ?-lactamase genes were absent in all isolates.By PCR amplification,DNA sequencing and BLASTn comparison analysis,the CARB genes of 2 strains were demonstrated to be CARB-3 and the VIM genes of 2 strains were VIM-2. CONCLUSIONS P.aeruginosa carries various beta-lactamase genes in clinical PAE patients,and the deletion ratio of oprD2 gene is high.

Objective To observe the effects of selenium extracellular polysaccharide of Porphyridium sp(Se-PSP) on the growth and apoptosis of BEL-7402 tumor cell line.Methods The Se-PSP was obtained from a sort of culture medium with some selenous acid solution.In vitro,human tumor cells were cultured in sorts of PSP or Se-PSP liquors with different concentrations,and the result of which was determined by MTT,Focus apoptosis assay and DNA fragmentation.Results and Conclusion Se-PSP(80mg?L-1) displayed strong growth inhibitory effects against BEL-7402 human cancer cell line,and induced the apoptosis of BEL-7402 cells.

Objective To study the effects of the inopolysaccharide from Spirulina maxima (IPSⅡA) on tumor-bearing mice.Mothods The sarcoma 180(S 180) was transplanted in the abdominal cavity and armpit of mouse and then the survival time, the tumor weight, leukocyte count and the immunity index of the tumor-bearing mice treated with IPSⅡA were measured and compared with the control group. Results and ConclusionThe results showed that medication of IPS IIA ip in advance could obviously inhibit the tumor growth and prolong the survival time of the tumor-bearing mice.

Objective: To a evaluate the effectiveness of comprehensive nutrition interventions among primary school students in Yiwu City, so as to provide insights into malnutrition control among children. Methods: Grade 3 to 5 students were sampled from four primary schools in Yiwu City using a random cluster sampling method and randomly assigned into the intervention group and the control group. Students in the intervention group received comprehensive interventions, including nutritional health education, promotion of physical activities, nutritional meal support and creation of a nutritional campus, while students in the control group were given no interventions. The awareness of nutritional health knowledge, dietary behaviors and nutritional status were compared in students between the two groups prior to interventions and one year following interventions, and the effectiveness of interventions was evaluated using generalized estimating equations. Results: Totally 879 students were enrolled. There were 440 students in the intervention group, including 243 males and 197 females, with a male to female ratio of 1︰0.81 and a mean age of (10.47±0.99) years; and there were 439 students in the control group, including 244 males and 195 females, with a male to female ratio of 1︰0.80 and a mean age of (10.35±1.02) years. Following comprehensive interventions, the awareness of “type of food”, “seven nutriments from food”, “eating at least 12 types of food daily”, “less than 6 g of daily salt intake”, “food composition in nutritional breakfast”, “nutritional labels of pure milk”, “no less than 60 min of daily exercise duration” and “too fat or too thin may threaten health” and the increase in the proportion of 3 and more types of food in breakfast were significantly higher among students in the intervention group than in the control group (all P<0.05); however, there was no statistical difference in the proportion of normal nutrition between the two groups (P>0.05). Conclusion The comprehensive nutritional interventions may effectively increase the awareness of nutrition health knowledge and improve dietary behaviors among primary school students.

BACKGROUNDThe use of oxaliplatin is very little in the treatment of lung can- cer . This study is designed to find out the efficacy and toxicity of the combination of oxaliplatin and gemcitabine in the treatment of advanced non-small cell lung cancer (NSCLC).

METHODSThere were 32 cases of advanced NSCLC patients who were treated with oxaliplatin 65mg/m² by intravenous infusion on the 1st and 8th days, and gemcitabine 800-1000mg/m² by intravenous infusion on the 1st and 8th days every 21 days. After 2 cycles the efficacy was evaluated.

RESULTSThere were 31 cases that could be evaluated. No complete response was observed and the overall response rate was 22.6% (7 partial response). The disease control rate was 71.0%. The median time to diease progression was 7 months and the median survival time was 14.5 months. The 1-year survival rate was 59.0% and 2-year survival rate was 45.8%. The main toxicities were myelosuppression and nausea and vomiting.

CONCLUSIONSThe combination of oxaliplatin and gemcitabine is effective and well tolerated in the treatment of advanced NSCLC. The quality of life is little influenced and the regimen also can prolong the survival time in some patients.

Objective To investigate the effects of methylprednisolone on the release of cytokines in patients after successful cardiopulmonary resuscitation(CPR).Method Thirty patients after CPR with restoration of spontaneous circulation(ROSC)were randomly divided into two groups:group A(methylprednisolone group,n=14)and group B(control group,n=16)during the period from May 2005 through May 2007.The patients of group were treated with methylprednisolone 3 mg/kg by intravenously twice a day after ROSC.The levels of serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-10 (IL-10)were measured by enzyme-linked immunosorbent assay(ELISA)before CPR,and 24,48,72 hours and 7 days after ROSC.The data were analyzed studentis t test and chi-gquare test.A P value less than 0.05 indicated significant difference.Results There was on significant difference in the mean time from cardiac arrest to return of spontaneous circulation,and the levels of serum cytokines between the two groups before CPR(P＞0.05).In comparisorl with group B,the levels of serum TNF-α,IL-1β,IL-6,IL-8 decreased markedly at 24 and 48 hour after ROSC in group A(P＜0.05-0.01),and the levels of serum IL-8 decreased markedly at 72 hours after ROSC in groupA(P＜0.05).Thelevels of serum TNF-α,IL-1β,IL-6,IL-8 were not of significant differences between the two groups at 7 hys after ROSC(P＞0.05).There was no significant difference in the levels of serum IL-10between the two groups at different time points after ROSC(P＞0.05).Conclusions Methylprednisolone plays a role of preventive effects on patients with ROSC after PCR through decreasing the levels of serum TNF-α,IL-1β,IL-6,IL-8.