Objective To study the change of serum procalcitonin(PCT) expression level and to investigate its clinical value for predicting the prognosis in the patients with severe acute pancreatitis (SAP) .Methods Totallay 48 inpatients with SAP in our hos‐pital from April 2012 to April 2014 were retrospectively analyzed .The PCT levels during different hospitalization period in the sur‐vial group and the death group were detected and analyzed .The scores of acute physiological and chronic health evaluation Ⅱ (A‐PACHE Ⅱ) were compared between these two groups .Results (1)The clinical characreristics of gender ,body temperature and WBC had no statistically significant differences beween the survival group and the death group (P>0 .05) ,but the PCT level had statistical differencet (P<0 .05) .(2)There was no statistically significant difference in the PCT level and APACHE Ⅱ score at ad‐mission between the two groups .In the later treatment process ,the PCT level and APACHE Ⅱ score in the survival group were progressively decreased ,while which in the death group were progressively inecreased (P<0 .05) .WT5HZ〗Conclusion PCT has an important clinical value in forcasting the prognosis of SAP .

OBJECTIVE: To provide references for optimizing the selection of medical insurance drugs and drug review in China.METHODS: The Canadian common drug reviewers' basic duties and the Canadian common drug review process were introduced,and the existing problems in drug review and the countermeasures were analyzed.RESULTS & CONCLUSIONS: In view of the problems occurred in Canadian drug review such as redundant delay and tedious,lack of transparence and job responsibility system and so on,the corresponding measures had been taken such as defining drug review time,helding long range e-meeting,and establishing public committee etc.Chinese common drug review should draw experiences from the Canadian drug review to explore a more economical,timely and transparent practice in Chinese medical insurance drug selection and drug review.

Objective:To identify potential risk factors of suicide among schizophrenic inpatients,in order to provide basis of how to prevent suicide in psychiatric inpatients.Method:A retrospective analysis among inpatients with mental disorders in Guangzhou psychiatric hospital from 1956 to 2005,the first psychiatric hospital,in China.Using a case-control design,sixty four schizophrenic inpatients(suicide group) who committed suicide in psychiatric hospital and the same number of randomly selected control subjects(control group) who had no incident of suicide were selected and their psychiatric records were compared.Results:The rate of suicide was 133.1(95% CI 103.4-62.9) per 100 000 admissions.Sixty four cases(83.1%) were diagnosed as schizophrenia,fifty nine(76.6%) were hung by themselves,and fifty two(67.5%) happened in wards.The numbers of hospitalization and the suicide attempts before and duration of hospitalization were significantly higher in suicide group than that in control group(P=0.001,0.017,0.003),and guilty thoughts,depressive mood were significantly higher in suicide group than that in control group(P=0.006,0.004).Both suicidal ideation and suicide attempts one month before and during hospitalization were significantly higher in suicide group than that in control group(P

OBJECTIVE To study resistance and the change of Pseudomonas aeruginosa of respiratory system infection,and instruct the antibacterial medicine to use rationally.METHODS The respiratory system infection patients from Jun 2004 to Dec 2005 were investigated.To the results of MICs with 229 P.aeruginasa strains were analyzed.RESULTS From inspected 5411 phlegm samples derived from Jun 2004 to Dec 2005,bacteria from 2291 samples(42.34%) were examined out,from them 229 strains(10.00%) were P.aeruginosa. The sensitive rate to tobramycin,imipenem/cilastatin sodium,piperacillin sodium,amikacin sulfate,ceftazidime,cefoperazone/sulbactam(Sulperazon),et al was greater than 60%.CONCLUSIONS We should consider the mechanism of P.aeruginosa resistance in treatment of respiratory system infection patients,and guide the management of antibacterial using.

ObjectiveTo observe the status and related factors of depression symptoms in elders.MethodsRandomized multi-stage sampling method was used to select subjects.504 elders ( aged ≥65 years) in Jining were investigated and assessed by using the scales of Geriatric Depression Scale15 ( GDS-15 ),Katz-activity of daily liying (Katz-ADL) and Lawton instrumental ADL (IADL).ResultsThe GDS scores of males (2.24 ±2.12 ) were significantly lower than that of females ( 3.35 ± 3.49) ( t '=4.236,P ＜ 0.01 ).There were significant differences in GDS scores among different subgroups of age,single or not,physical activity,sleep quality,self-reported health,living condition,economic status and lonely feeling.GDS scores were significantly correlated to lonely feeling,IADL,living condition,economic status,self-reported health and age (F =27.47,P ＜ 0.001 ; R2 =0.372,R'2 =0.358 ),with the total contribution of 35.8％.ConclusionAmong elders,the main factors influencing depression symptoms are lonely feeling,IADL,female,health condition,economic condition and single,respectively.Enhancing IADL of elders can contribute to reduce the risk factors of depression.

Objective To investigate status about the heart rate variability and executive function in patients with insomnia.Methods The study involves 165 cases of patients with insomnia and 92 cases normal.The Pittsburgh Sleep Quality Index (PSQI) screening for insomnia,Wisconsin Card Sorting Test (WCST) for the assessment of executive function and Self-generate Physiological Coherence System (SPCS) were used to collect the data of heart rate variability (HRV).Results The SDNN,RMSSD,TP,LF,HF of HRV of the insomnia group were significantly higher than these factors of control group (t=2.712,2.726,2.427,2.542,2.338,P＜0.05 or P＜ 0.01).In the WCST,number of completed categories,conceptualization percentage of the group of patients with insomnia were significantly lower than the control group (t=-2.368,-2.165,all P＜0.05),persistent errors,persistent error percentages,continuous number of responses of patients' group were significantly higher than the control group (t=2.712,2.991,2.690,all P＜0.01).TP,LF,HF,LF/HF of HRV and the number of error responses,persistent errors,persistent error percentages,continuous number of responses of WCST had significant negative correlations (r=-0.171--0.245,P＜0.05 or P＜0.01),while the conceptualization percentage were significantly positively correlated (r=0.198-0.227,P＜0.05 or P＜0.01).Conclusion The results show that insomnia not only cause individual autonomic disorders,but damage the executive function of prefrontal.HRV and executive function in patients with insomnia have close relationships.

Objective To elucidates the effects of HPV18 E6 siRNA targeting at human papillomavirus(HPV)18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity.Methods HPV18 E6 expression of HeLa cells was inhibited by siRNA interference,the change of P53 and P21 proteins expression level was measured by Western blot.MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome of HeLa cells.Results After inhibition of E6 expression,P53 and P21 proteins increased and the growth of HeLa cells was decreased(P ＜0.01).The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome.Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit proliferation of HeLa cells.HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.

Objective To study the inclusion mechanism of 14-deoxyandrographolide and ?-cyclodextrin in inclusion ratio,molecular interaction between host and guest,inclusion part and stability of inclusion compound.Methods The formation of inclusion compound was confirmed by dfferential thermal analysis(DTA);The equilibrium constants of inclusion compound were determined and the thermodynamic constants were calculated by UV;Inclusion parts were confirmed by IR and 1H-NMR.Results Ka=1 292.631 L/mol;?G=-16.854 8;?H=-25.532;?S=-0.029 12.It was clearly that all five lactone ring and decalin ring of 14-deoxyandrographolide in indusion compound changed in IR and 1H-NMR.Conclusion Inclusion host-guest ratio is 1∶1,Van der Waals forces play a major role in the process.Inclusion site is decalin ring and five lactone ring.The inclusion compound is more stable under normal temperature.

OBJECTIVE To explore associated factors of nosocomial infection and provide ways for control nosocomial infection.METHODS The associated factors of nosocomial infection were analyzed by using retrospective analysis in 29 842 cases from Jan 2004 to Dec 2005.RESULTS Among 29 842 cases,1917 cases were infected,the incidence was 6.4%.CONCLUSIONS The associated factors of nosocomial infection are age,the underlying diseases,the decrease in immunological function and the invasive manipulation.

OBJECTIVE To undersand neurosurgical hospital infection characteristics and bacterial resistance,and to provide reference information for the rational use of antibacterial drugs in clinics.METHODS Using the combined methods of initiative monitoring and recalling system to carry on the clinical informations' statistics,the analysis and the judgment for the 880 inpatients in the neurosurgery department in 2005.RESULTS The rate of neurosurgical hospital infection was 16.36%,significantly higher than the hospital average rate(9.18%) at the same period.Neurosurgical hospital infection pathogens were mainly Gram-negative bacteria.Infection sites occurred mainly in urinary tract(44.44%),followed by lower respiratory tract(35.42%).Sixty strains were Escherichia coli(20.2%),34 strains(11.44%) for Enterococcus faecium(D group),25 strains(8.42%) for Acinetobacter baumannii,25 strains(8.42%) for Pseudomonas aeruginosa,and 18 strains(6.06%) for Klebsiella pneumoniae.CONCLUSIONS Systematic monitoring of neurosurgical hospital infection characteristics and dynamics of bacterial resistance has important clinical reference significance to the rational use of antibacterial drugs.