This study examined the inhibitory effect of topical cyclosporine (CsA) treatment on conjunctiva epithelial apoptosis in a murine model of xerophthalamia. Dry eye was induced in 3 groups of C57BL6 mice by subcutaneous injection of scopolamine (t.i.d) and exposure to an air draft and low-humidity environment for 16 h each day for 12 days. The dry eye control group received no topical treatment; another group received 1 microL of 0.05% CsA topically (t.i.d, dry eye+CsA); and the third group received 1 microL of the castor oil vehicle of CsA topically (t.i.d, dry eye + vehicle). Normal mice were used as untreated controls. Twelve days later, the mice were killed, and their conjunctivas were excised. The number of the conjunctival goblet cells was counted in tissue sections stained with periodic acid Schiff (PAS) reagent. Their conjunctiva epithelium had been investigated by immuno-histochemical staining to detect the goblet cells and the expression of Caspase-3, Bax and bcl-2. Our results showed that compared with dry eye control and dry eye mice + vehicle groups, the number of conjunctival epithelial goblet cells was significantly greater in the untreated controls and dry eye mice receiving CsA (P < 0.01 for both groups). There was no significant difference in the number of conjunctival epithelial goblet cells between the dry eye control and dry eye+vehicle group. It was also true of the number of conjunctival epithelial goblet cells when comparison was made between the normal group and the dry eye+CsA group. Expressions of Caspas-3 and Bax were increased and ex-pression of bcl-2 was decreased in conjunctival epithelial cells in dry eye control and dry eye mice+vehicle groups. There was a significant positive correlation between goblet cell number and the number of cells that expressed bcl-2, and a negative correlation between goblet cells and Caspase-3 and Bax expression. It is concluded that the topical use of CsA could significantly reduce conjunctival epithelial apoptosis and protect goblet cell against the loss in experimental murine xerophathalamia. Inhibition of apoptosis appears to be a key mechanism responsible for the therapeutic effect of CsA on xerophthalamia.

Objective To study the effect of letrozole plus metformin on infertility of polycystic ovary syndrome(PCOS).Methods According to the digital table,90 patients with infertility of PCOS were randomly divided into the control group(n =45 cases) and the treatment group(n =45 cases).The patients of the control group were treated though clomiphene,while the patients of the treatment group were treated though letrozole plus metformin.The ovulation rate,pregnancy rate,sex hormones and insulin resistance were detected.Results The homeostasis model assessment of insulin resistance index (HOMA-IR) was (5.72 ± 2.37) before treatment and (3.16 ± 1.39) after treatment in the treatment group.HOMA-IR was (5.69 ± 2.40) before treatment and (4.81 ± 2.06) after treatment in the control group.There was a significantly decrease in the treatment group (t =3.7246,P ＜ 0.05).The ovulation rate was 80％ in the treatment group,while 60％ in the control group,the difference between the two groups was significant(x2 =4.06,P ＜0.05).The pregnancy rate was 48.9％ in the treatment group,while 28.9％ in the control group,the difference between the two groups was significant (x2 =5.77,P ＜ 0.05).Conclusion Letrozole plus metformin could promote the ovulation and decrease insulin resistance.

Objective To establish stable insect cell line expressing mIL4 on the basis of construction of inset expression vector pIZT/V5-His.Methods Amplified mIL-4 and pIZT/V5-His were treated with EcoRI and XbaI,and mIL-4 were ligated into pIZT/V5-His vector using T4 DNA ligase.Sf9 cells were transfected with recombinant DNA and ELISA was employed to detect soluble mIL-4 production by transfected Sf9 cells.Results Transfected Sf9 cells could significantly produce mIL4(1 mg?L~(-1)) compared!with control group(0.003 mg?L~(-1)).Conclusion Inset expression vector pIZT/V5-His is an ideal expression vector for mIL-4 production in transfected cells.

OBJECTIVE:To establish and compare the HPLC characteristic Spectrum Erycibe obtusifolia from different habi-tats. METHODS:HPLC was performed on the column of Hypersil ODS C18 with the mobile phase of acetonitrile-0.1% phosphoric acid at flow rate of 1.0 ml/min,detection wavelength was 347 nm,the column temperature was 30℃and the sample size was 20μl. 12 different batches of samples were determined and the characteristic spectrums of those were established. TCM fingerprint evalu-ation software(2004 edition)was used to evaluate the similarity of 12 batches of samples. RESULTS:10 characteristic peaks were identified in the characteristic spectrum of E. obtusifolia. Peak 3 was scopolamine glycosides,peak 4 was chlorogenic acid and peak 6 was scopoletin. The similarities of 10 batches of samples were proved to be higher than 0.90 and 2 batches were proved to be less than 0.90. CONCLUSIONS:The method is simple,accurate and reproducible,and can provide scientific evidence for effectively controlling the internal quality standards.

Objective To detect the correlation between the periodontal bone loss and carotid calcifications by using MSCT.Methods Imaging materials of 270 patients who were suspected atherosclerosis and underwent MSCT were retrospectively studied.According to the score of carotid calcification,the patients were divided into three groups:slight calcification group (carotid calcification score <100),severe calcification group (carotid calcification score ≥100),and normal control group (carotid calcification score=0).The original images were post-processed with volume rendering(VR),multi planar reformation(MPR),curved planar reformation(CPR)and maximum intensity projection (MIP)by using EBW4.5 workstation,and then the residual teeth number and the periodontal bone loss teeth were recorded in different groups.Results On MSCT,the carotid calcification score and the number of periodontal bone loss were displayed clearly.The number of the periodontal bone loss teeth in the calcification groups were significantly higher than those in the normal control group(P<0.01).Furthermore,the number of the periodontal bone loss teeth was positively correlated with the severity of carotid calcification.Conclusion The periodontal bone loss is highly correlated with the severity of the carotid calcification.The volume reconstruction technique of MSCT is a non-invasive diagnostic testing to detect the periodontal desease and to quantify carotid calcifications.

Objective To observe the effect of Ruzengping granule on mammary gland of rat with mastoplasia. Methods After benzoate estradiol and progestogen had been injected into rats for 35 days, the mastoplasia model was made. The model rats were treated with Ruzengping granule for 30 days, to measure the mammary papilla diameter of the rats in all groups, to observe morphologic changes of mammary papilla and the changes of mammary gland under microscope, to determine the content of estradiol in blood serum and blood rheology by radio-immunity. Results Ruzengping granule could significantly inhibit the mammary papilla's diameter of the mastoplasia rats, lessen mammary papilla’s symptoms in morphology, reduce the quantity of lobule of mammary gland, lobule-alveolus in mammary gland and the diameter of acinar lumina. Ruzengping granule could also significantly improve blood rheological nature and degrade content of estradiol in blood serum. Conclusion Ruzengping granule can healing efficaciously rat’s mastoplasia induced by benzoate estradiol.

Objective To explore the influence of different domains of STAT6 expressed by the vector pGEX-6p-2 on signal transduction.Methods Different domains of STAT6 were amplified by PCR technique and ligated respectively into the prokaryotic expression vector.Recombinant DNA was transformed into HB101 cells.Transformants were lysed by supersonic method and lysates run on SDS-PAGE.Results Western blotting analysis showed a high level expression(60 000,56 000 and 50 000) of different domains of STAT6 SHST,DST and NST,and the empty vector only had GST expression.Conclusion Expression vector pGEX-6p-2 can be used for high level expression of different domains of STAT6.

Objective To identify the role of protein kinase C(PKC) activation in ischemic myocardium proctection and its effect after inhibition by polymyxin B sulfate.Methods We randomly divided 30 rabbits into ischemic(IS) group,ischemic preconditioning(IPC) group and polymyxin B sulfate(PMB) group.According to the method reported in previous literature,we simultaneously recorded ECG,pressure curve and monophasic action potential(MAP) throughout the ischemia and reperfusion perocess.Results The rabbit heart rates were not changed significantly during the ischemic or reperfusion period.The products of heart rate multiplying systolic pressure were significantly higher in IPC group compared with the other two groups(P

Objective To investigate the polymorphism of the vitamin D receptor gene (VDR)TaqⅠin relation to diabetic retinopathy. Method Fragment length discrepant allele specific PCR(FLDAS-PCR) were used to determine VDR genetypes in 158 patients with diabetic retinopathy and in 198 normal subjects. Results The frequency distribution of VDR genotypes in diabetic retinopathy patients was 106 (67.1%) in TT, 33(20.9%) in Tt, 19(12.0%) in tt; and in normal persons was 165 (83.3%) in TT, 23(11.6%) in Tt, 10 (5.1%) in tt. There was a significant difference between diabetic retinopathy patients and normal persons in distribution of VDR gene TaqⅠgenotypes(P

OBJECTIVE: To provide references for optimizing the selection of medical insurance drugs and drug review in China.METHODS: The Canadian common drug reviewers' basic duties and the Canadian common drug review process were introduced,and the existing problems in drug review and the countermeasures were analyzed.RESULTS & CONCLUSIONS: In view of the problems occurred in Canadian drug review such as redundant delay and tedious,lack of transparence and job responsibility system and so on,the corresponding measures had been taken such as defining drug review time,helding long range e-meeting,and establishing public committee etc.Chinese common drug review should draw experiences from the Canadian drug review to explore a more economical,timely and transparent practice in Chinese medical insurance drug selection and drug review.