1.hsa-miR-124-3p.1 inhibits the migration and invasion of human gastric cancer cells by targeting TRAF6
Zhenggui TAO ; Jinghu DU ; Kui TIAN ; Donghua WANG ; Fengqi HU ; Manyu CHEN
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(6):843-849
【Objective】 To observe the effects of hsa-miR-124-3p.1 in inhibiting epithelial-mesenchymal transition (EMT), migration and invasion of human gastric cancer cells induced by transforming growth factor β1 (TGF-β1) by targeting tumor necrosis factor receptor-associated factor 6 (TRAF6). 【Methods】 A total of 43 gastric cancer tissues and 43 normal para-carcinoma tissues were collected. The human gastric mucosal epithelial cells GES-1 and gastric cancer cells (NCI-N87, MGC-803, BGC-823, SGC-7901, and MKN-45) were cultured. The expressions of miR-124-3p.1 and TRAF6 in tissues and cells were detected by fluorescent quantitative PCR and Western blotting. The targeted relationship between miR-124-3p.1 and TRAF6 was verified by dual-luciferase reporter gene system assay. SGC-7901 cell lines with miR-124-3p.1 and TRAF6 overexpression were constructed. The cells were induced by TGF-β1. The invasion and migration abilities of the cells were evaluated by Transwell chamber assay and scratch test. 【Results】 Compared with normal para-carcinoma tissues and normal gastric mucosal cells, the expression of miR-124-3p.1 was downregulated, while the expressions of TRAF6 mRNA and protein were upregulated in gastric cancer tissues and cells (P<0.05). Compared with control group, expression of E-cadherin in cells was downregulated, expressions of N-cadherin and Vimentin were upregulated, invasion and migration rates of cells were increased in TGF-β1 group (P<0.05). Compared with TGF-β1 group, after cells were transfected with miR-124-3p.1 mimic, the expression of E-cadherin was upregulated, the expressions of N-cadherin and Vimentin were down-regulated, and invasion and migration rates of cells were decreased (P<0.05). Compared with miR-124-3p.1 mimic group, invasion and migration rates of cells were increased in TGF-β1+mimic+TRAF6 group, expressions of TRAF6, N-cadherin and Vimentin were up-regulated, and the expression of E-cadherin was down-regulated (P<0.05). 【Conclusion】 hsa-miR-124-3p.1 is lowly expressed in gastric cancer. Overexpression of miR-124-33p.1 can inhibit EMT, cell invasion and migration induced by TGF-β1. And the action mechanism may be related to the downregulated expression of TRAF6.