Acute myeloid leukemia is a group of malignant tumor characterized by chromosome abnormality and/or gene mutations.Its pathogenesis is very complex.Gene mutations in IDH1/2 which encode metabolic enzyme were found by sequencing recently.This discovery aroused interest in the domestic and foreign research group.A series of clinical and basic research was also conducted.This review is based on these studies.

0.05). CONCLUSION: Cefepime is a safe and effective agent in treatment of low-respiratory tract infection like ceftazidme.

OBJECTIVE To investigate the efficacy of rneropenem for the meatment of the patients with serious community-acquired pneumonia(SCAP).METHODS Sixty-four patients with SCAP were randomly divided into two groups.Group A(the treatment group,41 cases) was treated with meropenem(5-1.0 g iv q8-12 h?7 d) and group B(the control group,23 cases) treated with mezlocillin(5-1.0 g iv q8-12 h?7 d) in an open clinical study.RESULTS The efficacy rate of group A was 85.0%,with a bacteria elimination rate of 86.4% wheveas 57.0% and 64.3% in group B.There were significant difference between two groups(P

Cholangiocarcinoma (CCA) is a group of solid tumors with high malignant degree and poor prognosis. Surgical resection has still been the main therapy options. Targeting therapy and immunotherapy are the main anti-tumor methods that have been paid more and more attention in recent years, especially immunotherapy. The tumor microenvironment of CCA is complex, which encompasses not only interstitial and endothelial cells, but also a large number of immune cells. In addition, the innate and adaptive immune systems also play a role. This article summarizes the immune-related studies of cholangiocarcinoma and the latest clinical trials of immunotherapy.

Objective To estimate the efficacy and safety of cryopreserved platelets in surgical patients.Methods 238 patients in recent years with hemorrhage or high risk of hemorrhage induced by thrombocytopenia or functional platelet defects during or after surgery, who had been transfused with cryopreserved platelets, were included in our study. The following items were recorded: bleeding time before and two hours after transfusion of cryopreserved platelets; the persist time of blood oozing from wound after transfusion; platelet count before and one hour after transfusion; platelet count before and immediately after surgery; and the number of patients with adverse transfusion reactions. The hemostatic efficacy was judged by the bleeding time and platelet count change.Results Bleeding times before and two hours after cryopreserved platelets transfusion were (9?3)min,(4?2)min respectively; blood oozing from wound stopped 0.5～2 hours after transfusion; the platelet level was elevated by(5～42)?10 9/L one hour after transfusion; the postoperative platelet count was decreased by(130?69)?10 9/L compared to preoperative count; four patients had urticaria and five had febrile reaction. The hemostatic efficacy was 95%(226/238) Conclusion Cryopreserved platelets function immediately to bring about the cessation of blood oozing from the wound,and elevation of peripheral blood platelet count.

Objective To identify clinical prognostic predicators and surgery indicator of stage IIIb-T_4 in patients with lung cancer patients whose tumor invaded heart, main vessel or carina. Methods From 1988 to 2000,151 patients with pathological stage IIIb-T_4 lung cancer who underwent resection by Shandong Provincial Hospital were analyzed as control samples, 112 patients with pathological stage IIIa-T_3N_1M_0 who underwent resection at the same time were also analyzed. Factors were estimated from the date of operation using the Kaplan-Meier and Log rank analysis. The Cox regression model evaluated the influence of factors on the survival. Results The median survival period were 26.1 months and the overall of 1-year, 3-year and 5-year survivals of the 151 patients were 73.5%, 33.1% and 16.6% respectively.The significant prognostic factors (P

Objective To select the reversible activation inhibitors for platelet retreating before lyophilization.Methods Effects of phyticacid and bivarudin of different concentration on platelets in vitro viability and functional activity were evalued by the maximal aggregation of platelets,CD62p and PAC-1 expressions and re-expressions stimulated by thrombin,and platelets SPAT.Results Expression of CD62p and PAC-1 increased after treatment.Phyticacid with a concentration of 1mmol/L could decline the PAC-1 expression,but could not decline the re-expression of CD62p and PAC-1,and the aggregation reaction induced by propylgallate was also maintained.Bivarudin at a concentration of 1mmol/L could inhibit both expressions(0.78% and 0.24%) and re-expressions(7.00% and 14.39%) of CD62p and PAC-1.Aggregation reactions stimulated by inducers except thrombin were maintained.With phyticacid concentration ≤2mmol/L,the aggregation time was prolonged;while the aggregation time was the same with ≤ 3?mol/L bivarudin.Conclusions Phyticacid with the concentration of 1mmol/L could inhibit the GPIIb/IIIa conformation change of platelet,while 1?mol/L Bivarudin could inhibit platelet activation,and a better protection will be observed with a lower concentration.

Objective To explore the factors affecting the collection of allogenic peripheral blood stem cells in donors. Methods Using COBE-spectra blood cell separator to collect peripheral blood stem cells in 21 allogenic donors. The impact of the factors including the number of white blood cell(WBC) and nucleated cell(NC), and mono-nucleated cell(MNC) ratio on the collecting rate of peripheral blood stem cells was analyzed before and after collection. The output of CD 34 +stem cells stimulated by different doses of G-CSF was also analyzed. Results When the mean count of peripheral WBC and MMC in donors was (41?7.7)?10 9/L and 12?2.9% respectively, the count of collected NC was 2.7?0.8?10 8/L, and the number of the collected CD 34 + stem cells was also higher. Statistical analysis showed that the number of NC was significantly related to the collecting rate of CD 34 stem cells(P

In this study, cryopreservated platelets were transfused to 1560 patients. of them, 536 were patients with acute leukaemia, 285 with anemia, 253 with thrombocytopenia due to chemical or radicalized treat, 438 in perioperation, and 48 with DIC or hemorrhage of rupture esophagus varix. The storage time of cryopreserved platelets ranged from one week to thirteen months. Most of the cryopreserved platelets had not been washed before application and no obvious adverse-effects were observed except for garlic smell in patients' exhalation after transfusing cryopreserved platelets. The results showed that transfusion of cryopreserved platelets has a remarkable on effect hemostasis and can enhance the platelet count in peripheral blood. Compared with liquid-preserved platelets, cryopreserved platelets for clinic use have the following advantages:1) the potentiality of large stock, 2) much longer shelf life, 3) higher safety, 4) superior hemostasis effect, and 5) capacity to meet emergent massive clinic demand.

Objective To discuss the content determination method ofα-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3μg (r=0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery ofα-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.