BACKGROUND: Bone morphogenetic protein-2 has been previously proved to not only stimulate and different bone tissue-derived cells, but also induce differentiation from cell strain into osteoblasts; however, direct application of bone morphogenetic protein has poor effects on repairing bone defects. OBJECTIVE: To study new bone formation in a rabbit model of avascular necrosis of the femoral head (ANFH) following recombinant human bone morphogenetic protein-2 (rhBMP-2)/fibrin sealant (FS) implantation combining with core decompression. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force from January 2005 to December 2007. MATERIALS: Composite was made by rhBMP-2 and FS, and the final concentration of rhBMP-2 was 1 mg/L. METHODS: Animal models of ANFH were made by injecting hormone. The rabbits were randomly divided into three groups, including rhBMP-2/FS implantation group, rhBMP-2 implantation group, and core decompression alone group. MAIN OUTCOME MEASURES: Signal changes of femoral head and sclerotin were detected using MRI method; new bone formation was observed under optic microscopy; calcium content was measured using atomic absorrtion spectrophotometer. RESULTS: MRI indicated that new bone replaced primary bone defect channel at week 8 after rhBMP-2/FS implantation. A few of new bones were observed in the rhBMP-2 implantation group, and fiber tissue was still observed in the core decompression alone group. Morphology suggested that a great quantity of mature bone trabecula and plate-shaped bone replaced primary bone defect channel at week 8 after rhBMP-2/FS implantation. Bone defect was decreased in the rhBMP-2 implantation group, accompanying with a few of bone trabecula and blood capillary but a large quantity of fiber tissues. At week 8 after core decompression alone, bone defect was decreased, and a few of new bones were observed. Fiber tissue still existed in the center, and any bone tissue did not fill in it. Calcium content in the rhBMP-2/FS implantation group was greater than rhBMP-2 implantation group and core decompression alone group (P

Adult ; Arsenic Poisoning ; etiology ; Arsenicals ; Humans ; Male ; Skin ; injuries ; Skin Absorption ; Wounds and Injuries

Objective:To investigate the risk factors for intracranial hematoma progression in patients within 24 h of traumatic brain injury.Methods:A prospective study was performed; 184 patients with traumatic brain injury admitted to our hospital from January 2018 to June 2021 were enrolled. According to the states of intracranial hematoma indicated by head CT within 24 h of injury, these patients were divided into intracranial hematoma progression group ( n=52) and intracranial hematoma stable group ( n=132). The clinical data of patients in the two groups were compared and the independent risk factors for intracranial hematoma progression were screened by multivariate Logistic regression analysis. Results:As compared with intracranial hematoma stable group, patients in the intracranial hematoma progression group had significantly advanced age, significantly higher systolic blood pressure and blood glucose levels, statistically higher proportions of patients with parenchymal hemorrhage, subarachnoid hemorrhage, and multiple hematomas, significantly longer prothrombin time, significantly higher international standardization index and D-dimer level, significantly higher proportion with patients with fibrinogen<2.0 g/L, statistically increased K value (blood coagulation time) of thromboelastic map, proportion of patients with α Angle (blood coagulation angle)<64°, level of vascular endothelial biomarker syndecan-1 (Syn-1), and von willebrand factor (vWF) activity, and significantly decreased Glasgow Coma Scale (GCS) scores at admission and platelet count ( P<0.05). Multivariate Logistic regression analysis showed that age ( OR=1.066, 95%CI: 1.018-1.117, P=0.007), systolic blood pressure ( OR=1.076, 95%CI: 1.041-1.111, P<0.001), multiple hematoma ( OR=6.559, 95%CI: 2.025-21.245, P=0.002), fibrinogen<2.0 g/L ( OR=6.164, 95%CI: 1.586-23.954, P=0.009), K value ( OR=6.500, 95%CI: 1.755-24.082, P=0.005) and Syn-1 level ( OR=1.111, 95%CI: 1.015-1.215, P=0.022) were independent risk factors for intracranial hematoma progression in patients with traumatic brain injury at early stage. Conclusion:Traumatic brain injury patients, at early stage, with advanced age, multiple intracranial hematoma, high systolic blood pressure, low fibrinogen, prolonged K value and high Syn-1 level are trend to have intracranial hematoma progression.