Objective To study pathologic changes of gastric mucosa in duodenal ulcer(DU).Methods Pathologic changes of 650 cases of gastric mucosa in DU confirmed by pathology after subtotal gastrectomy were analyzed.Results There were 100% patients with complication of gastritis.Among them,506% patients accompanied with gastric mucosa chronic atrophic gastritis and 209% patients with gastric mucosa intestinal metaplasia.Conclusion DU is always accompanied by antral gastritis,the pathologic changes of gastric mucosa in DU relateed tightly with helicobacter pylori(Hp) infection and patient's age.

Objective To investigate the pathogenesis pathology,CT diagnosis and differential diagnosis of hydranencephaly.Methods CT manifestations and clinical data of 9 cases with hydranencephaly were analysed retrospectively.Results CT findins of 9 cases were almost defects of bilateral cerebral hemisphere and ventricle.Only a little remainder of occipital,frontal and/or temporal lobes were appearence.The structure of subtentorium was normal.Conclusion CT scan directly shows all appearances of hydranencephaly and is great valuable for diagnosing this disease.

Objective To explore the role of Cyclin B1 and Cyclin D1 on Barrett esophagus,Barrett's esophagus mixed with atypical dysplasia and esophageal adenocarcinoma.Methods Cyclin B1 and Cyclin D1 were examined with immunohistochemistry.76 esophageal tissues of patients werB collected,including severe reflux esophagitis(RE,25 cases),Barrett esophagus(BE,35 cases),Barrett esophagus mixed with atypical dysplasia(DY,8 cases),esophageal carcinoma(CA,8 cases).Ten cases with normal esophageal mucosa were examined as the control. Results Cyclin B1 and Cyclin D1 were high expression in the specimens of the BE,DY and CA groups and very low expression in the control and RE group.Statistieal difference Was showed(P<0.01).Expression of Cyclin D1 was increasing gradually from the tissues of intestinal metaplasia,atypical dysplasia to adenocarcinoma(50.04 vs 67.94 vs 74.31).There Was significant difference among these three groups(P<0.01).Conclusion Cyclin B1 and Cyclin D1 as markers of tumour development could evaluate the risk from Barrett esophagus to adenocarcinoma.Perhaps it is the earlv event in the development of esophageal carcinoma.

Objective To develop a method for the determination of 11 perfluorosulfonate and perfluorocarboxylate precursors in eggs using ultra-high performance liquid chromatography-tandem quadruple mass spectrometry (UPLC-MS/MS).Methods The target compounds of egg were extracted with 100 mmol/L NaOH-acetonitrile/water(90:10,V/V) by ultrasonic.Then the extract was purified by solid phase extraction (Waters Oasis@WAX 6cc) and then eluted with 9％ NH4OH in methanol.The target compounds were separated on a Waters ACQUITYTM BEH 18C column (50 mm × 2.1 mm,1.7 μm) and detected by negative electrospray ionization (ESI-) mass spectrometry in multiple reaction monitoring mode (MRM).All compounds were quantified with internal standards.The accuracy,precision and the limits of detection and quantification of the method were evaluated.Then we detected 7 different egg samples from the market.Results The average recoveries for the eleven precursors at 3 levels were 74.09％-116.82％ and the relative standard deviations were 2.37％-13.62％.The limits of detection (LOD) of the method were in the range of 0.06-1.50 pg/g (wet weight) and the limits of quantification (LOQ) were in the range of 0.15-3.00 pg/g (wet weight).And 5 target compounds were detected in the 7 market samples.6:2 fluorotelomer sulfonate (6:2 FTS) was detected in all of samples with the concentrations of 1.67-3.11 pg/g.6:2fluorotelomer unsaturated acid (FHUEA) and 6:2 disubstituted polyfluoroalkyl phosphate ester (6:2 diPAP) were detected in 6 samples and the concentrations were＜LOD-5.11 pg/g and 3.78-9.16 pg/g,respectively.And the concentrations of 8:2 fluorotelomer sulfonate (8:2 FTS) and N-methyl perfluorooctane sulfonamidoacetic acid (N-Me FOSAA) founded in the same sample were 105.78,and 4.95 pg/g,respectively.Conclusion This method was simple,rapid,and suitable for determination of perfluorosulfonate and perfluorocarboxylate precursors in eggs with high accuracy and sensitivity.It could also be applied to human burden studies of these precursors.

Objective To develop a method for the determination of 11 perfluorosulfonate and perfluorocarboxylate precursors in eggs using ultra-high performance liquid chromatography-tandem quadruple mass spectrometry (UPLC-MS/MS).Methods The target compounds of egg were extracted with 100 mmol/L NaOH-acetonitrile/water(90:10,V/V) by ultrasonic.Then the extract was purified by solid phase extraction (Waters Oasis@WAX 6cc) and then eluted with 9％ NH4OH in methanol.The target compounds were separated on a Waters ACQUITYTM BEH 18C column (50 mm × 2.1 mm,1.7 μm) and detected by negative electrospray ionization (ESI-) mass spectrometry in multiple reaction monitoring mode (MRM).All compounds were quantified with internal standards.The accuracy,precision and the limits of detection and quantification of the method were evaluated.Then we detected 7 different egg samples from the market.Results The average recoveries for the eleven precursors at 3 levels were 74.09％-116.82％ and the relative standard deviations were 2.37％-13.62％.The limits of detection (LOD) of the method were in the range of 0.06-1.50 pg/g (wet weight) and the limits of quantification (LOQ) were in the range of 0.15-3.00 pg/g (wet weight).And 5 target compounds were detected in the 7 market samples.6:2 fluorotelomer sulfonate (6:2 FTS) was detected in all of samples with the concentrations of 1.67-3.11 pg/g.6:2fluorotelomer unsaturated acid (FHUEA) and 6:2 disubstituted polyfluoroalkyl phosphate ester (6:2 diPAP) were detected in 6 samples and the concentrations were＜LOD-5.11 pg/g and 3.78-9.16 pg/g,respectively.And the concentrations of 8:2 fluorotelomer sulfonate (8:2 FTS) and N-methyl perfluorooctane sulfonamidoacetic acid (N-Me FOSAA) founded in the same sample were 105.78,and 4.95 pg/g,respectively.Conclusion This method was simple,rapid,and suitable for determination of perfluorosulfonate and perfluorocarboxylate precursors in eggs with high accuracy and sensitivity.It could also be applied to human burden studies of these precursors.

OBJECTIVETo study the application of hand-assisted laparoscopic surgery (HALS) in colorectal carcinoma resection.

METHODSFourteen patients with colorectal carcinoma underwent resection by HALS.

RESULTSHand-assisted laparoscopic right hemicolectomy was performed in 6 of 8 ascending colon cancer patients, spending an average of 160 minutes (110 to 220 minutes) for the procedure. The amount of bleeding was 40 approximately 100 ml. Bowel sounds resumed in 36 approximately 72 hours after the operation. The average interval between the operation and patients' discharge was 8 days (7 to 12 days). For 2 patients the surgeons had to resort to laparotomy. All patients received postoperative chemotherapy. Hand-assisted laparoscopic abdominoperineal resection was performed in 5 of 6 patients with lower rectal cancer, with the average duration of 180 minutes (120 to 270 minutes), with bleeding of 40 to 80 ml. Bowel sounds resumed within 24 to 72 hours and the average discharge interval was 14 days (12 to 18 days). The urinary catheter in 6 patients was removed in 7 to 8 days after the operation. All patients with rectal carcinoma received postoperative chemotherapy and radiotherapy. One patient was shifted to laparotomy because of extensive adhesion in the pelvic cavity. Four of 11 patients treated by HALS experienced slight pain in the incision. No other complications were observed in any of the patients. Remote results await further follow-up.

CONCLUSIONHand-assisted laparoscopic surgery in the treatment of colorectal carcinoma, a safe and simple procedure, not only ensures the clearance of tumor, but also reduces operative trauma and hastens recovery.

Adult ; Aged ; Colectomy ; Colorectal Neoplasms ; surgery ; Female ; Hand ; Humans ; Laparoscopy ; Male ; Middle Aged ; Treatment Outcome

Objective:To understand the current situation of the construction of occupational health information system in China and put forward countermeasures and suggestions for the construction of occupational health information system.Methods:In November 2019 and April 2020, a cross-sectional survey was conducted on the occupational health information systems of national, provincial and some central enterprises. A total of 57 occupational health information systems were investigated, including 4 national systems, 44 provincial systems and 9 industrial systems. The terminal type, main function, construction type, user classification and main authority, computer terminal structure, data collection mode, data transmission mode, data docking are analyzed.Results:The four national level systems all adopt B/S structure, and all transmit network data through computer terminals. The main data acquisition mode is online acquisition and external system docking. Among the 44 provincial-level systems, 41 (93.18%) were self built systems, 75.00% (33/44) were computer terminals, and 90.91% (40/44) were B/s structures; 17 (38.64%) systems used multiple data acquisition methods; 13.64% (6/44) systems used multiple data transmission methodsand the main way of data transmission method is network report (88.64%, 39/44) ; 84.09% (37/44) of the system network access mode was Internet mode. Among the nine industry systems, 66.67% (6/9) of them deployed servers in the form of self built computer rooms, 77.78% (7/9) of them supported docking and reserved ports; 66.67% (6/9) of them used computer terminals, and 100.00% (9/9) of them used B/S structure; 77.78% (7/9) of them used manual filling for data collection.Conclusion:The construction of occupational health information system in China has many problems, such as scattered and separate construction, and lack of effective data sharing between related systems, it＇s urgent to unify the standard and plan as a whole.

Objective:To understand the current situation of the construction of occupational health information system in China and put forward countermeasures and suggestions for the construction of occupational health information system.Methods:In November 2019 and April 2020, a cross-sectional survey was conducted on the occupational health information systems of national, provincial and some central enterprises. A total of 57 occupational health information systems were investigated, including 4 national systems, 44 provincial systems and 9 industrial systems. The terminal type, main function, construction type, user classification and main authority, computer terminal structure, data collection mode, data transmission mode, data docking are analyzed.Results:The four national level systems all adopt B/S structure, and all transmit network data through computer terminals. The main data acquisition mode is online acquisition and external system docking. Among the 44 provincial-level systems, 41 (93.18%) were self built systems, 75.00% (33/44) were computer terminals, and 90.91% (40/44) were B/s structures; 17 (38.64%) systems used multiple data acquisition methods; 13.64% (6/44) systems used multiple data transmission methodsand the main way of data transmission method is network report (88.64%, 39/44) ; 84.09% (37/44) of the system network access mode was Internet mode. Among the nine industry systems, 66.67% (6/9) of them deployed servers in the form of self built computer rooms, 77.78% (7/9) of them supported docking and reserved ports; 66.67% (6/9) of them used computer terminals, and 100.00% (9/9) of them used B/S structure; 77.78% (7/9) of them used manual filling for data collection.Conclusion:The construction of occupational health information system in China has many problems, such as scattered and separate construction, and lack of effective data sharing between related systems, it＇s urgent to unify the standard and plan as a whole.

Objective:To evaluate the effect of transcutaneous electrical acupoint stimulation (TEAS) on the intracranial pressure in patients undergoing laparoscopic hysterectomy by measuring the optic nerve sheath diameter (ONSD) via ultrasound.Methods:Forty-two American Society of Anesthesiologists Physical Status classification Ⅰ or Ⅱ patients, aged 18-60 yr, with a body mass index of 18-28 kg/m 2, scheduled for elective laparoscopic hysterectomy, were divided into 2 groups ( n=21 each) using a random number table method: control group (group C) and TEAS group (group T). In group T, TEAS was applied to the Yintang (EX-HN3) and Taiyang (EX-HN5) acupoints at 30 min before anesthesia, the stimulation used alternating dense-disperse waves at a frequency of 2/100 Hz, with the current intensity starting at 1 mA and increasing to the maximum tolerable level just below the pain threshold, and stimulation was maintained at the Hegu (LI4), Quanliao (SI18), and Fengchi (GB20) acupoints during surgery until the procedure was completed. Patients had electrode pads applied to the corresponding acupoints without electrical stimulation in group C. The ONSD was measured upon entry into the operating room (T 0), 3 min after anesthesia induction (T 1), 5 min after trendelenburg position (T 2), 30 min after trendelenburg position (T 3), 60 min after trendelenburg position (T 4), and immediately after the end of operation (T 5). Results:Compared to the baseline at T 0, the ONSD was significantly increased at T 3-5 in group C and at T 4, 5 in group T ( P<0.05). The ONSD was significantly lower at T 4, 5 in group T than in group C ( P<0.05). Conclusions:TEAS can reduce the intracranial pressure to some extent in patients undergoing laparoscopic hysterectomy.

Objective To obtain representative data on levels of indicator polychlorinated biphenyls (PCBs) in foods consumed by the general population and to estimate the dietary intake of indicator PCBs in China. Methods The food samples were collected during the fifth China Total Diet Study (2009-2013). Based on the geographical location and dietary habits, China was divided into the south area and the north area, and 10 province regions from each area were chosen. In each province region, one urban site and two rural sites were selected to collect food samples. Considering the food consumption level and the PCBs contaminate rule, a total of 160 samples including meat, eggs, fish, milk, cereals, beans, potatoes and vegetables were selected. The concentration of 7 indicator PCBs in food were determined by stable isotope dilution gas chromatography-mass, and combined with food consumption to calculate the dietary intake of indicator PCBs. Results The concentration of indicator PCBs in 8 categories of food were in the range of 0.8-1 300.1 pg/g. The levels of indicator PCBs were significantly higher in the aquatic products, averaging (307.8 ± 302.4) pg/g, followed by eggs at (76.6 ± 92.1) pg/g and meat at (63.0 ± 54.9) pg/g. The daily dietary intake of indicator PCBs varied from province to province, ranging from 0.13 ng·kg-1·d-1 to 3.58 ng·kg-1·d-1, averaging (0.67 ± 0.77) ng · kg-1 · d-1. Fujian had the highest level(3.58 ng · kg-1 · d-1), followed by Shanghai (1.48 ng · kg-1 · d-1)and Zhejiang(1.09 ng · kg-1 · d-1). Compared with the minimum risk level (MRL) value (20 ng · kg-1 · d-1)proposed by US Agency for Toxic Substances and Disease Registry, the highest dietary intake level was only 17.9%MRL, the average dietary intake level was 3.4%MRL. Aquatic products was still the major contributor to the dietary intake of indicator PCBs in China, 48%of average dietary intake level (0.32 ng·kg-1·d-1/0.67 ng·kg-1·d-1). Conclusion The dietary intake of indicator PCBs in China was at a low level, and showing a declining trend.