The food poisoning caused by Bacillus cereus occurs in Kunming or Yunnan Province sometimes.The aim of our survey is to prevent and control this kind of food poi- soning in the future.The result shows that the positive rate is 19.20% and the degree of contamination differs in the different kinds of food,the dairy products being contaminated most seriously of all.The bacteria in the contaminated food number mostly 10~2～10~4 per gram,Though it is not very high,the presence of Bacillus cereus is so wide that we showld pay much attention to it. Abut 97.65% of Bacillus cereus can be biologically classified and the most com- mon types are 2,8 and 10.About 92.98% of Bacillus cereus can be classified according to the serological type,and type 3 is the most predominant one and type 1 the second.In the dairy products,biological types 2 and 8 make up 54.55% and serological type 1 36.36%. The bacteria isolated from the food are highly sensitive to erythromycin,chlor- amphenicol,gcntamycin,streptomycin and kanamycin.

Objective To establish a reverse transcription loop-mediated isothermal amplification RT-LAMP rapid detection method of enterovirus 71(EV71) in hand ,foot and mouth disease (HFMD) ,and to evaluate its application.Methods Four types of LAMP primers were designed by using the software needle based on the 6 distinct regions of the specific gene of EV71.The process of amplification was completed in the ordinary thermostatic water container by 65 ℃ for 1200 min.The results of amplification were judged by electrophoresis and naked-eye.Seventy EV71 type intestinal positive specimens were simultaneously detected by RT-LAMP and RT-PCR methods.EV71 type of intestinal virus RNA were made a series.After 10 times of dilution ,the RT-LAMP and RT-PCR methods were used to conduct the detection for comparing their sensitivities.Results The LAMP characteristic ladder bands of EV71 appeared ,then the results could judged by the naked-eye.The detection rate in 100 EV71 samples had no statistical difference between RT-LAMP and RT-PCR methods (P>0.05).The sensitivity (10.0 pg/μL) of RT-LAMP was same to that of RT-PCR method.Conclusion The RT-LAMP detection method for EV71 was established ,which can be used for nucleic acid am-plification in the ordinary thermostatic water container.The preliminary application verifies that this RT-LAMP assay has a certain application prospect.

Objective To discuss the relationship between and value of Leptin with disorder of lipid metabolism in gestational diabetes mellitus. Method Research objects are divided into three groups including GDM group, normal glucose tolerance group (NGT group), normal non-pregnant group. Blood lipid, fasting blood glucose, fasting insulin, serum leptin and placental leptin levels were detected and HOMA-IR was calculated. Result (1) Serum leptin, TC, TG, placental leptin and HOMA-IR in GDM group were higher than those of NGT group and TC, serum leptin and HOMA-IR of NGT group were higher than those of control group. There were statistical significances (P<0.05). (2)Serum leptin levels were positively correlated with BMI, HOMA-IR, TC and TG respectively (r = 0.593, 0.688, 0.722, 0.492 respectively, P < 0.05). Conclusion Leptin may cause lipid metabolism disorders, and together they participate in the pathogenesis of GDM. Maternal serum leptin level is expected to become an observation index of GDM.

objective To understand the clinical features of type 2 diabetes mellitus (T2DM) combined with metabolic syndrome (MS) in elderly patients, as well as the medicine used for treatment and the distribution of abnormal metabolic components. Methods Totally 349 T2DM patients admitted from 2001 to 2005 were retrospectively studied, among them 194 patients were combined with MS. The clinical data of those 194 patients were analyzed to evaluate their clinical feature, medicine used and the distribution of several abnormal metabolic components. of T2DM combined with MS. Results The proportion of MS in the elderly T2DM patients was 55.6%. Body mass index (BMI), postprandial blood glucose (PBG), blood pressure and triglyceride (TG) were higher than that in those patients of T2DM without MS, while the high density lipoprotein cholesterol (HDL-C) was lower (P

OBJECTIVE To investigate the resistance of Staphylococcus aureus,includ MRSA and MSSA,to antimicrobial agents in intensive care unit(ICU),and to provide basis for clinical therapy.METHODS K-B disk diffusion method was used to determine the drug susceptibility of 171 strains of S.aureus,and meticillin-resistant S.aureus(MRSA) was detected by using the disk diffusion method according to NCCLS/CLSI.RESULTS In ICU,S.aureus was isolated mostly from the respiratory tract,urine tract and blood.MRSA made up 57.89%.For MRSA and MSSA,no resistance against vancomycin and norvancomycin was observed.The resistance rates of MRSA to other agents were as follows: chloramphenicol 20.45%,and minocycline 20.45%.While the resistance rates of MSSA to other agents were as follows:cefazolin 6.25%,and amikacin 6.25%,minocycline 9.38%.CONCLUSIONS MRSA shows the multidrug resistance.Pathogenic monitoring,keeping the patients apart,rational utilization of antibiotics,medical facility sterilization strictly and intensive hand hygiene are very important to prevent MRSA infection.

Objective To evaluate quantitative examination of urinary sediment bacteria as a basic feasibility of screening indicators for urinary tract infection .Methods 191 outpatients and inpatient specimens were gathered firstly to implement a urine culture ,and then the rest of the urine were used for sediment bacteria quantitative testing .Meanwhile ,bacterial culture was conducted as the standard .According to the results of bacterial culture ,receiver operating characteristic(ROC) was drawn ,the threshold values of leukocyte and bacteria counts for diagnosis of urinary tract infection were found out and its sensitivity ,specificity ,positive / negative predictive value ,false positive/false negative rate and accuracy were calculated .Results The positive rate of urine culture was 39 .7% ,and the most common pathogen was Escherichia coli .The threshold value of bacteria and leukocyte counts for diagnosis of urinary tract infection was 1 024 .5/μL and 135 .8/μL respectively .When combined leukocyte and bacteria counts for urinary tract infection ,the optimum sensitivity was 62 .5% ,specificity was 98 .1% ,positive predictive value was 95 .7% ,negative predictive value was 79 .6% ,false positive rate was 1 .9% ,false negative rate was 37 .5% ,and accuracy was 83 .8% .Conclusion With UF‐1000i urinary sediment analyzer ,the combined determination of leukocyte and bacteria counts can remove the great mass of negative results ,Especially the results of bacterial culture positive predictability is higher ,but still can not replace of quantitative bacterial culture .

Objective To study physiological foundation of acupunctureDeqi.Methods 28 healthy volunteers who never experienced acupuncture were recruited. The right Zusanli (ST36) and a site 1.5 cm beside Zusanli (ST36) (nominated as A) were acupunctured at different time. Shallow and deep acupuncturing was inserted at Zusanli (ST36). Only deep acupuncturing was inserted at A. Hemodynamic parameters of the anterior tibial artery including the peak value in the systolic phase (Vmax), the average blood flow velocity (Vm), and the inner diameter of vessels (Dist) were observed with colored Doppler ultrasound before, at the end of acupuncture, and 3 min afterDeqi response. Results There was significant difference in those parameters between before acupuncture and 3 min after deqi response when deep acupuncturing at Zusanli (ST36) (P＜0.05), but was not significant difference when shallow acupuncturing (P＞0.05). There was significant difference in ΔVmax and ΔVm between acupuncture at Zusanli (ST36) and A points (P＜0.05). Conclusion Arterial haemodynamics variation seems to be associated with acupunctureDeqi.

Objective To evaluate the value of color Doppler ultrasonography for diagnosis of superior mesenteric artery diseases. Methods78 patients with abdominal pain suspected to be superior mesenteric artery diseases underwent color Doppler ultrasonography. The results were compared with other imaging methods or findings in operation. Results4 patients with superior mesenteric artery embolization coincided with the founding in operation. 18 of the 22 patients suggested with superior mesenteric artery stenoses were confirmed by CTA or DSA. ConclusionColor Doppler ultrasonography can be used in diagnosis of superior mesenteric artery diseases.