Objective To investigate the causes of and observe the therapeutic effect of microecological preparation on recurrent vulvovaginal candidiasis.Methods 576 patients with recurrent vulvovaginal candidiasis(RVVC) were tested in the papers and the laboratory,and 226 patients were divided into two groups randomly.Control group was treated with anti-fungus drug.The treatment group was treated with microecological preparation combined with anti-fungus drug.The therapeutical effects were observed at the 1st week,1st month and 2nd month after treatment respectively.Results Factors causing were as follows:use of broad-spectrum antibiotics(30%),infection of other pathogens(25%),washing vagina usually(19.1%).Anti-fungus drug was effective in the treatment of RVVC.The effective rate of the treatment group was 95.6%,the control group was 77.0% at 2nd month after treatment.There was significant difference between the treatment group and the control group(?~2=16.61,P

Objective To study the relationship between blood lipid levels, insulin therapy of pregnant women with abnormal glucose metabolism and perinatal outcomes. Methods 380 pregnant women who were diag-nosed and treated for abnormal glucose metabolism were enrolled in this study,including 24 cases with diabetes melli-tus(DM),164 eases with gestation diabetes mellitus (GDM), 192 cases with gestation impaired 81ucose tolerance (GIGT). Among the 380 cases, 136 cases were performed insulin therapy with diet controlling,244 cases diet control-ling. Results The incidence of pre-eclampsia and preterm labor in the DM group(41.7% and 41.7% )was higher than that in GIGT group(15.6% and 20.7%)and GDM group(15.9% and 18. 3%) (P<0.05). Among the three groups ,the incidence of macrosomia, polyhydranmios and fetal distress had no significant difference( P>0.05 ). While the incipience of neonatal asphyxia,hypoglycemia in the DM group were all higher than that in GDM and GIGT group ( P<0.01 ). The newborn weights in insulin therapy group( 2891.5±1330.4 g) was lower than that in diet controlling group(3751.2±1025.3 g) ( P<0.05 ). Conclusion The blood lipid level of pregnant women with gestational ab-normal glucose metabolism is one of the effective indexes to prognose perinatal outcomes. Reducing blood lipid levelcan decrease the incidence of pre-eclampsia and preterm labor,neonatal asphyxia and hypoglycemia significantly. Di-agnosing and beginning therapy as soon as possible and using insulin are important,especially to reduce the rate of macrosomia and newbem weights.

Objective To explore the clinical value of cervical lesion screening,which was detected by Pap smears,thinprep cytologic test (TCT),full automatic cellular DNA quantitative analysis system,human papilloma virus detection,colposcopy staining biopsy and blind biopsy.Methods 8 780 peoples were as the study object,divided into four groups,positive rate and accuracy of different screening methods;In addition,which were the positive rate of colposcopy staining biopsy and blind biopsy,the positive rate of HPV in all positive biopsy people and negative biopsy people,and the HPV infection rate of various lesions in positive patients were compared,respectivey.Results (1) The positive rates between the four groups showed an increasing trend (P ＜ 0.05).The positive rate of Pap smears and TCT had no statistical difference between every two of them (P ＜ 0.05),the rest were statistically significant (P ＜0.05).(2) The accuracy rates between the four groups showed an increasing trend (P ＜ 0.05).The accuracy rate of Pap smears and TCT had no statistical difference between two of them(P ＞ 0.05),the rest were statistically significant (P ＜ 0.05).(3)The positive rate of colposcopy biopsy under vision inspection with acetic acid (VIA) was higher than that of blind biopsy (P ＞ 0.05).(4) The positive rate of HPV detection of positive biopsy people was higher than that of negative biopsy people(P ＜0.05).(5)In colposcopy biopsy under vision inspection with acetic acid(VIA)group,the HPV positive rates of inflammation showed an increasing trend(P ＜ 0.05) ;in blind biopsy group,the HPV positive rates also showed an increasing trend (P ＜ 0.05).Conclusion (1) HPV test has the highest sensitivity and strongest specificity,cellular DNA quantitative analysis is second,Pap smears is worst,so HPV and DNA detection have more valuable in clinical.(2)There is no significant difference between colposcopy biopsy under vision inspection with acetic acid(VIA)and blind biopsy in clinical,but HPV infection is the key factor,which affects the occurrence,development of cervical intraepithelial neoplasia and gradually progresses to cervical cancer.

Objective To compare the clinical effect of three different methods in treatment of premature.Methods 150 premature patients were randomly divided into three groups,50 cases for each group,received treatments with ritodrine,magnesium sulfate plus salbutamol,or magnesium sulfate alone respectively. The tensity of uterine contraction, the interval between drug administrations and the relief of uterine contraction, the accumulative drug using time, the elongated pregnancy durations were observed for the dertermination of effectiveness. Results The group treated with ritodrine, magnesium sulfate plus salbutamol or magnesium sulfate were effective in 94%, 78%,70% of patients of each group, and it showed that ritodrine was more effective than the combination and magnesium sulfate. The intervals of drug using and contraction relief were statistically different among three groups, (2. 16 ±0. 91)h,(4. 14 ± 1.07)h,(5.12 ± 1.45) h for each(all P ＜0.05).The cumulative drug using times were(7. 04 ±1. 16) d, (7.46 ± 1.20) d, (7.06 ± 2.78) d for three groups and proved not to be statistically significant ( all P ＞0. 05 ). The elongated pregnancy durations were (22.08 ± 7.32 ) d, ( 17.42 ± 5.85 ) d and ( 11.82 ± 5.04 ) d, and significantly different in three groups ( all P ＜ 0. 05 ). Conclusion The treatment of premature with ritodrine or the combination of magnesium sulfate and salbutamol was superior to using magnesium sulfate alone.

Objective To establish a reverse transcription loop-mediated isothermal amplification RT-LAMP rapid detection method of enterovirus 71(EV71) in hand ,foot and mouth disease (HFMD) ,and to evaluate its application.Methods Four types of LAMP primers were designed by using the software needle based on the 6 distinct regions of the specific gene of EV71.The process of amplification was completed in the ordinary thermostatic water container by 65 ℃ for 1200 min.The results of amplification were judged by electrophoresis and naked-eye.Seventy EV71 type intestinal positive specimens were simultaneously detected by RT-LAMP and RT-PCR methods.EV71 type of intestinal virus RNA were made a series.After 10 times of dilution ,the RT-LAMP and RT-PCR methods were used to conduct the detection for comparing their sensitivities.Results The LAMP characteristic ladder bands of EV71 appeared ,then the results could judged by the naked-eye.The detection rate in 100 EV71 samples had no statistical difference between RT-LAMP and RT-PCR methods (P>0.05).The sensitivity (10.0 pg/μL) of RT-LAMP was same to that of RT-PCR method.Conclusion The RT-LAMP detection method for EV71 was established ,which can be used for nucleic acid am-plification in the ordinary thermostatic water container.The preliminary application verifies that this RT-LAMP assay has a certain application prospect.

Objective To evaluate the effects of controlling arterial oxygen partial pressure (PaO 2) at the beginning and during cardiopulmonary bypass (CPB) on cyanotic myocardial protection. Methods 40 Children suffering from tetralogy of Fallot(TOF) under 3 years of age with SpO 2

Objective To evaluate the results of direct anastomosis between the main pulmonary artery and right ventricle in the repair of complex congenital heart disease. Methods Between March 2001 and June 2004, seventeen patients with complex congenital heart disease underwent direct anastomosis between the main pulmonary artery and right ventricle. Among them, eleven patients were under 3 years old. Six patients had pulmonary atresia and ventricular septal defect (VSD), 4 had transposition of the great arteries and VSD, 4 persistent truncus arteriosus and 3 TOF with coronary artery straddling RVOT. Twelve patients underwent one stage complete corrective repair and 5 patients received staged procedures. Results There was 1 operative death with hospital mortality of 5.9%. Three out of 5 staged patients succeeded in the second corrective repair. At follow-up ranging from 1 to 40 months, all patients were growing well and their heart function were in NYHA class 0 or I. Conclusion Direct anastomosis between the main pulmonary artery and right ventricle can provide good results in the repair of certain complex congenital heart disease, especially in infant patients without suitable homograft.

Objective To evaluate the outcomes of one-stage repair of aortic coarctation (CoA) and associated cardiac anomalies in infants through a midline sternotomy. Methods Between June 2002 and August 2005, 36 patients (27male, 9 female) underwent surgery for CoA. The age was from 18 days to 8 years[mean (14.3?9.5) months], 27 patients (75%) were younger than 1 year and 18 patients (50%) were younger than 3 months. The body weight was from 3.0 to 23 kg [mean(6.6?3.0)kg]. 27 patients were symptomatic infants and 8 patients were asymptomatic of adults. One patient had recoarctation after repair through a left thracotomy one year ago. The associated cardiac anomalies included ventricular septal defect, patent ductus arteriosus, secundum atrial septal defect, hypoplasia of the aortic arch, transposition of great arteries and subaortic stenosis. 36 cases underwent one-stage operation of extended end-to-end anastomosis through a midline sternotomy. Results One patient died postoperatively with a mortality of 2.8 %. The cause of death was heart and lung function failure. 35 cases were followed up from 4 months to 3 years .One late death occurred. 34 cases were in good condition and no recoarctation. Conclusion Outcomes of one-stage repair of CoA and associated cardiac anomalies in infants through a midline sternotomy is excellent. Technique of extended end -to-end anastomosis may potentially reduce the incidence of recoarctation.

BACKGROUND: Central oxytocin (OT) may be a neurotransmitter or neuromodulator and play an important role in learning and memory, sexual behaviour, pain modulation and opiate tolerance and dependence. To research the interactions between oxytocinergic and opioidergic system in hippocampus has some significance.OBJECTIVE: To investigate the effects of OT administered intracerebroventricularly on evoked discharge of left dorsal hippocampal CA1 neurons in rats and the possible interactions between oxytocinergic and opioidergic system.DESIGN: A randomised controlled study.SETTING: Department of Physiology of Guangdong Medical College; Department of Physiology and Pathology of Medical College of Wuhan University.MATERIALS: The study was conducted in the Physiology Department of Medical College of Wuhan University from September 2002 to September 2003. A total of 36 male Sprague-Dawley rats were selected and randomly divided into six groups: control (NS), OT groups (0.2 mg/L, 2 mg/L and 20 mg/L), [d (CH2)5-OVT]+OT (2 mg/L), naloxone+OT (2 mg/L), with 6 rats in each group.METHODS: Single-unit recording was performed with glass microelectrode. The glass microelectrode was inserted by a micromanipulator into hippocampal CA1. The electrical activity was amplified by a microelectrode amplifier and then recorded by the biological experimental system,monitored at the same time with oscilloscope. When recording the neural discharge, electrical stimulation of the sciatic nerves was performed once 5minutes through a double stainless electrode. 5 μL oxytocin in dosage of 0.2, 2 and 20 mg/L were injected slowly into lateral ventricle via microlitre syringe. [d(CH2)5-OVT]+OT (2 mg/L) group: 2.5 μL [d(CH2)5-OVT](80 mg/L) was injected into lateral ventricle and then 2.5 μL oxytocin (2 mg/L). Naloxone+OT (2 mg/L) group: 2.5 μL naloxone (400 mg/L) was injected into lateral ventricle and then 2.5 μL oxytocin (2 mg/L). According to frequency of discharge, effect of oxytocin at various dosages on discharge induced by neurons in hippocampal CA1 area and [d (CH2)5-OVT]and naloxone on oxytocin was assayed. MAIN OUTCOME MEASURE: Changes of discharge frequency after stimulation.RESULTS: Data of totally 36 rats were entered the final analysis. ① OT (0.2 mg/L, 2 mg/L and 20 mg/L) administered by intracerebroventricularly could decrease the evoked discharge of hippocampal CA1 neurons in a dose-dependent manner. ② The inhibitory effects of OT (2 mg/L) could be blocked by pretreated intracerebroventricularly injection of [d (CH2)5-OVT](80 mg/L, 2.5 μL). ③ Intracerebroventricular injection of naloxone (400 mg/L, 2.5 μL) could attenuate the effects of OT (2 mg/L) significantly.CONCLUSION: OT can inhibit the electrical activities of hippocampal CA1 neurons to external electrical signal through activating the oxytocin receptor. Moreover, central opioid receptor is involving in the inhibitory effects of OT.

AIM: To establish an animal model for studying the development and metastasis of melanoma. METHODS: C57BL/6 mice were used as host to receive melanoma cell transplantation. Three kinds of melanoma cell lines, B16F0, B16F1 and B16F10, cultured to prepare the cell suspensions, were transplanted into the mouse anterior chamber (AC) of the eye. The time of eyeball diabross, time of survival and metastasis of lymph node and lung were observed. RESULTS: The time of eyeball diabross in F10 group was earlier than that in other groups. The time of eyeball diabross was no difference between F0 and F1 groups. Metastasis was developed 18 days after transplantation in F1 and F10 groups, where the tumor cells was found in ipsilateral cervical lymph nodes. The melanoma cells metastasized to lung in all three groups 28 days after transplantation. The survival time in F0 group was longer than F1 and F10 groups. There was no difference in survival times between F1 and F10 group. CONCLUSION: The differences of three kinds of melanoma cell lines in tumor development and metastasis provided the evidence that was useful for choosing suitable animal model further to study the eye melanima.