Objective To examine the expression of tribbles homologous 3 (TRB3) on lipopolysaccharide (LPS) induced acute lung injury (ALI) and its relationship with p38-mitogen-activated protein kinase (MAPK) pathway.Methods Rats received a intravenous injection by LPS (5 ml/kg) as models of ALI and a intravenous injection by NS (5 ml/kg) as the control.In rat lung tissue the expression of TRB3 protein was examined using immunohistochemical staining, the expression of TRB3 mRNA was determined by reverse transcript polymerase chain reaction (RT-PCR).Cultured rat pulmonary microvascular cells (PMVEC) were randomly divided into dose-dependent, time-dependent and intervention groups in vitro.In dose-dependent group, PMVEC were stimulated by various concentrations of LPS (0, 2, 4, 10 μg/ml) for 4 h, and in time-dependent group PMVEC were challenged by 10 μg/ml LPS for different time (0, 4, 8, 12 h).In intervention group, PMVEC grown in normal medium or medium with 10 μg/ml LPS for 4 h were pretreated using p38-MAPK inhibitor (10 μmol/L SB203580) for 2 h.Western blot was used to examine expression of TRB3, p-p38 and p38-MAPK.Results Immunohistochemical staining showed that TRB3 protein distributed in rat alveolar walls and glandular epithelium.Increased TRB3 mRNA expression using RT-PCR were found in lung tissue of rats injected by LPS when compared to those in NS group (t=15.524, P<0.01). Increased TRB3 mRNA expression using RT-PCR had also been found in PMVEC stimulated by LPS when compared to those in NS group (t=7.549, P<0.01). In PMVEC, LPS significantly increased the expression of TRB3 protein in a dose-dependent manner (0, 2, 4, 10 μg/ml) after stimulation for 4 h (F=12.619, P<0.001).At indicated time-points after PMVEC were challenged by 10 μg/ml LPS, the expression of TRB3 protein raised at 4 h , then decreased gradually at 8 h , but still was higher than 0 h group , there were significant difference (F=11.273, P<0.001). LPS significantly increased the expression of p-p38 protein after stimulation for 4 h when compared to the control group, LPS also increased the expression of TRB3 protein after stimulation for 4 h when compared to the control group (t=49.121,15.113,P<0.001). SB203580 decreased the protein levels of p-p38 in response to LPS, SB203580 also decreased the protein levels of TRB3 in response to LPS(t=7.040,11.900,P<0.05,0.001).SB203580 alone had no effect on the expression of p-p38 and TRB3,when compared to the control group,there was not statistically significant.Conclusion Levels of TRB3 protein increase in LPS-induced acute lung injury, and is regulated by p38-MAPK pathway.

Objective To evaluate the effects of different interventios on insulin resistance and ?-cell function in population with impaired glucose tolerance(impaired glucose tolerance,IGT).Methods The total of 382 cases of IGT were divided into five groups according to interventions:control(C),diet plus exercise group(D+E),Acarbose Group(A),Metformin(M)and traditional Chinese medicine Group(TCM),with each group making the appropriate intervention programmes.BMI,FPG,2hPG,F-Ins,P-Ins,F-CP and P-CP were determined before intervention and yearly during the follow up,and BMI,HOMA-IR,HOMA-? were calculated.Results By the end of the study,insulin,F-CP,IR Group D+E and FPG,2hPG,insulin,C-peptide,IR in the other groups were significantly lower than before the intervention(P

Objective To explore the effects of mobile platform in patients with surgery for pancreatic cancer. Methods From June 2016 to December 2017, we selected 78 inpatients with surgery for pancreatic cancer as subjects in He'nan Cancer Hospital. All of the patients were randomly divided into observation group and control group with the random number table, 39 cases in each group. Observation group provided health education by mobile platform. Control group used routine health education. And then, we compared the treatment compliance and self-care agency of patients in two groups after health education. Results Three months after intervention, the scores of dimensions in the treatment adherence questionnaire of patients in observation group were higher than those in control group with significant differences (P＜0.01). Three months after intervention, the total score and scores of dimensions in self-care agency questionnaire were higher than those in control group with significant differences (P＜ 0.01). Conclusions The application of mobile platform in health education among patients with surgery for pancreatic cancer can improve patients' treatment compliance and their self-care agency.