Objective To introduce the relationship between the apoptosis hepatocyte and its genic mediation and the ischemia of portal vein. Methods The combination of related literatures and our research findings were made.Results Portal vein ischemia may induced hepatocyte apoptosis, p53 and bcl-2 gene alternatively adjust hepatocyte apoptosis. Expression of p53 gene is enhanced in hepatic tissue when hepatocyte apoptosis is not obvious, but after 24-72 h of portal vein ischemia, when hepatocyte apoptosis is obvious, enhanced expression of p53 gene or reduced expression of bcl-2 gene occur. There exists close relationship between portal vein ischemia and hepatocyte apoptosis. Conclusion Apoptosis hepatocyte is involved in organic atrophy after ischemia of portal vein, and p53 and bcl-2 gene alternatively adjust hepatocyte apoptosis. At present, the mechanism of apoptosis of hepatocyte induced by ischemia of portal vein is not clear, which needs further study.

Objective To investigate the impact of sphingosine kinase 1 (SPK1) modified adipose tissue-derived stromal cells(ADSC)on tissue engineered bone osteogenesis.Methods ADSC cells isolated from SD rat fat cells were divided into CON group and SPK1 group,and then the cells were respectively infected with 10 MOI CON and SPK1 lentivirus for 48 h.The infection efficiency was confirmed by using flow cytometry.Alizarin red and oil red O was used to stain the cells 14 days after ADSC infection,and osteogenic and adipogenic ability was evaluated by detecting A595nm and A490 nm.In the meantime,the activity change of alkaline phosphatase(ALP)was detected.The SD rat femoral defect model was created,and then after combining ADSC with β-TCP,the tissue engineered bone was pressed to the defect site.The repairment of bone defect was detected by X-ray in 4,6 weeks.After infection of CON and SPK1 virus,bone morphogenetic protein(BMP7)expression in ADSC of these two groups was detected.Results The infection efficiency of CON and SPK1 lentivirus was 94.4％ vs.94.9％ respectively by flow cytometry.The SPK1 protein expression level in CON group and SPK1 group was (0.73±0.10) vs.(1.29±0.17)(P＜0.05).The A value of CON and SPK1 group at 595 nm was (0.20±0.02) vs.(0.41±0.01) (P＜0.05),respectively.The A value of CON and SPK1 group at 490 nm was (0.72±0.01) vs.(0.51±0.02)(P＜0.05),respectively.The expression level of ALP in CON and SPK1 group was (1.42±-0.09) vs.(2.68±0.09) (P＜0.01),respectively.In the repairment of bone defect,high density tissue at rat bone defect was significantly larger in SPK1 group than in CON group in 4 weeks,and in 6 weeks,bone defect of SPK1 group was healed,but CON group still had defect.The expression level of BMP7 in CON and SPK1 group was (1.13±0.16) vs.(4.46±0.23)(P＜0.05),respectively,48 h after infection.Conclusion SPK1 modified ADSC has an enhanced osteogenesis ability in vitro and in vivo,which was related to the activation of BMP7.

Chemotherapy plays an important role in advanced colorectal cancer. Patients may show various clinical response to selection different drugs. The importance of individual therapy based on pharmacogenomics has been emphasized in cancer therapy. To explore the individual therapy of colorectal cancer, the progress of molecular markers which influence the efficacy of anti-tumor drugs containing fluorouracil, oxaliplatin, CPT-11, monoclonal antibody drugs is reviewed in this article.

Tumor necrosis factor-related apoptosis inducing ligands (TRAILs) and their receptors can specifically express in hepatocellular carcinoma cells.TRAIL can specifically induce tumor cells apoptosis through death receptors (DRs) ; TRAIL combined radiation and chemotherapy can effectively overcome the TRAIL resistance of tumor cells,and promote the apoptosis of tumor cells,meanwhile the gene therapy of TRAIL on anti-tumor clinical treatment has been widely used.

Chemotherapy plays an important role in advanced colorectal cancer. Patients may show various clinical response to selection different drugs. The importance of individual therapy based on pharmacogenomics has been emphasized in cancer therapy. To explore the individual therapy of colorectal cancer,the progress of molecular markers which influence the efficacy of anti-tumor drugs containing fluorouracil,oxaliplatin,CPT-11,monoclonal antibody drugs is reviewed in this article.

Objective To study the diagnosis and treatment of abdominal aortic anurysm-intestinal fistula(AAA-IF). Methods The diagnosis and treatment of one patient with AAA-IF was reported, while a literateres review of AAA-IF was made. Results A 50 years old femal patient abmitted due to upper abdominal pain for two month, and an AAA was found in the explore laparotomy. The patient suddenly occurred severe upper abdominal pain accompanied with shock and massive upper gastrointestinal bleeding 13 days after the operation. The preoperative suspected diagnosis was AAA-IF. An AAA-IF was ound during emergent operation. AAA-IF resection with artifial vessel replacement, resection of the forth portion of duodenal and upper portion of jejunal were performed together. The patient recovered eventurely except high body temperature. After vigovous antibiotics therapy, the patient recovered. Conclusions AAA-IF is a rare disordor, the mortality can be as high as over 30%. The key points of redecing mortality are that the diagnosis and treatment should be in time; postoperative vigorous antibiotics should be given, because the AAA-IF patient tends to occur septic complications due to the communication occurred between the AAA and intestinal tract.

OBJECTIVE To explore the method and effect of endoscopic technique on maxillofacial benign tumor.METHODS Ten patients visiting our hospital from January to December 2007 and diagnosed as maxillofacial benign tumor were recruited in this study.The tumor of the patients were composed of 4 sebaceous glands,3 liparomphalus,3 epidermoid cysts.All of them were treated with endoscopic technique.The surgical approach was all in hair line or behind ear lobe and the incision was about 1cm. Subcutaneous tunnel was prepared.The operations were guided by the endoscope and helped with selfmade tritus skin expandor.RESULTS The operations were performed with an average duration of 45 minutes. Patients were all satisfied with the post operative results. CONCLUSION Endoscopic technique could well be applied in surgery of maxillofacial benign tumor.

[Objective]To evaluate the role of mobile bearing knee prosthesis in TKA and the clinical results.[Method]A total of 301 TKAs by using mobile bearing knee prosthesis were gotten average 48.7 months follow-up.HSS knee scores and X-ray films were to used to evaluate the results.[Result]The mean HSS score improved from 51.3 before operation to 91.3 after operation.The ROM improved from extension/flexion(-9.5?/64.3?)before operation to extension/flexion(0?/97.5?)after operation.The maximum varus/valgus(25?/15?)before operation were improved to the mean valgus 5.3?.Two cases of septic loosening and 2 cases of femoral fracture were found.[Conclusion]The CR type mobile bearing knee prosthesis not only had advantages in kinematics and wear experiments,but also had achived good clinical result.

cases with spondylotisthesis were treated with RF system. The types of spondylotisthesis were investigated and result was analysed. The results : follow up of an average of 5 years showed obvious relief of the symptoms. It suggested that RF system has the advantages of high reduction rate restoration of the intervertebral height and lumbo sacral physiological curvature and easy manipulation.

Objective To compare the laryngeal mask insertion conditions at different plasma target concentrations (Cp) during the induction of anesthesia with target-controlled infusion (TCI) of propofol.Methods Forty-five ASA I - II patients of both sexes aged 18-60 yr, weighing 50-80 kg undergoing minor surgery in which the use of laryngeal mask (LM) was indicated were randomly divided into 3 groups ( n = 15) according to Cp of propofol set during induction of anesthesia: 4, 6 and 8 ?g ? ml-1 . The patients were premedicated with intramuscular scopolamine 0.3 mg. Fentanyl 3 ?g?kg-1 was given i.v. . TCI of propofol was started 3 min later with Diprifusor (Graseby 3500 infusion pump). LM was inserted when the effect-site concentration (EC) of propofol reached 2.5 ?g ? ml -1 as displayed on the infusion pump. LM insertion conditions (mouth opening, gagling, coughing, head and limb movement, overall ease of insertion) were assessed. The total dose of propofol, insertion time, the time needed to reach EC 2.5 ?g?ml-1 were recorded. SP, DP, HR and BIS value were recorded at 6 time points: baseline before induction (T1 ) , at the loss of consciousness (T2), at EC 2.5 ?g ? ml-1 (T3), immediately (T4), 3 min (T5) after insertion of LM. Results The SP, DP, HR and BIS value were decreasing with increasing depth of anesthesia in the 3 groups. The decrease in BP and BIS value after insertion of LM was significantly larger in group 3 (8 ?g ?ml-1) than in group 1 and 2 (P