Objective To conclude the technology and clinical outcomes of percutaneous kyphoplasty for the treatment of osteoporotic vertebral compression fractures.Methods From June 2011 to June 2014,29 cases of osteoporoticthoracic and lumbar vertebral compression fractures patients,who were diagnosed by ultrasonic bone mineral density measurement.X-ray,CT and MRI were treated with percutaneous kyphoplasty in Beijing 401 Hospital of China Nuclear Industry.Observed and recorded the degree of pain,patient's activity and the recovery of vertebral height in patients with 24 hours,3 months and 6 months after operation.The index data were analyzed by SPSS 17.0 statistical software,and the measurement data were analyzed by paired t test.Results Twenty-nine patients were operated successfully,17 cases (58.62％)of back pain disappeared and 12 cases (41.38％) of back pain significantly reduced.Twenty-seven cases (93.1％) were allowed to get off bed after 24 hours of operation and 2 cases (6.9％) after 3 days.Three cases (10.34％) occurred PMMA leakage.After 24 hours,3 months and 6 months VAS and vertebral height of the original vertebral height percentages are much better than before surgery (P ＜0.01),after 3 months and 6 months VAS scores were significantly better than surgery after 24 hours (P ＜ 0.01),and with the extension of the observation,VAS scores decreased,but the degree of decline in after three months and six months was no statistical significance (P ＞ 0.05).Compared after surgery 3-month and 6-month with after surgery 24 hours,the vertebral height have lost,but the lost height of the vertebral was no statistical significance (P ＞ 0.05).No infection,hematoma,spinal nerve injury and PMMA poisoning complications occurred.Conclusions Percutaneous kyphoplasty can relieve the pain and restore part of the vertebral height satisfactory with better outcomes.

Objective To investigate the expression of heat shock protein-70(HSP70)in acute lung injury induced by paraquat poisoning.Methods A total of 72 Wistar rats were randomly divided into 3 groups(n=24 each group)and intraperitoneally injected with normal saline in group A,20 mg/kg paraquat in group B,and 40 mg/kg paraquat in group C.The lungs of rats were excised 1,3,6,12,and 24 hours after the injection,respectively.The expression of HSP70 in the lung tissue was detected by immunohistochemistry and Western blot.Results Paraquat induced acute lung injury in groups B and C.In group B,the expression of HSP70 increased at 2 hours,reached the peak at 6 hours,decreased at 12 hours,and returned to the baseline level at 24 hours.In group C,the expression of HSP70 increased at 1 hour,reached the peak at 3 hours,and returned to the baseline level at 24 hours.Conclusion Paraquat-induced acute lung injury may contribute to the enhanced expression of HSP70 in the bronchus,bronchiole,and alveolar epithelial cells,which indicates that HSP70 has a protective effect on lung injury.

The quality control process throughout the Ebola virus nucleic acid detection in Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab) was described in detail, in order to comprehensively display the scientific, rigorous, accurate and efficient practice in detection of Ebola virus of first batch detection team in SLE-CHN Biosafety Lab. Firstly, the key points of laboratory quality control system was described, including the managements and organizing, quality control documents and information management, instrument, reagents and supplies, assessment, facilities design and space allocation, laboratory maintenance and biosecurity. Secondly, the application of quality control methods in the whole process of the Ebola virus detection, including before the test, during the test and after the test, was analyzed. The excellent and professional laboratory staffs, the implementation of humanized management are the cornerstone of the success; High-level biological safety protection is the premise for effective quality control and completion of Ebola virus detection tasks. And professional logistics is prerequisite for launching the laboratory diagnosis of Ebola virus. The establishment and running of SLE-CHN Biosafety Lab has landmark significance for the friendship between Sierra Leone and China, and the lab becomes the most important base for Ebola virus laboratory testing in Sierra Leone.
China ; Ebolavirus ; classification ; genetics ; isolation & purification ; Hemorrhagic Fever, Ebola ; diagnosis ; virology ; Humans ; Laboratories ; manpower ; standards ; Laboratory Infection ; Quality Control ; RNA, Viral ; genetics ; Sierra Leone