Objective To explore the application of color Doppler ultrasound in prenatal diagnosis of fetal malformations.Methods 2764 cases of pregnant women giving birth were recruited as our subjects,who were hospitalized from Nov.2007 to Dec.2010.The color Doppler ultrasound instrument was used for fetal malformation diagnosis the analysis of color Doppler ultrasound of the practical application and testing accuracy were performed.Results Among 2764 pregnant women,13 cases with fetal malformations were determined by color Doppler ultrasonography detected.All these 13 cases of fetal malformation were confirmed later.Certainly,there are 2 cases of fetal malformations were not detected by color Doppler ultrasonography,of which one case was congenital heart disease and other was chest deformity.The accuracy rate of color Doppler ultrasonic detector was 86.67％.Conclnsion Color Doppler ultrasound might be a high accuracy detection methods with simple and lower cost in the prenatal diagnosis of fetal malformations.

Objective To objectively access and investigate the value of ultrasound technology in the diagnosis of breast cancer.Methods Fifty patients who underwent two-dimensional ultrasound or color Doppler ultrasound examination at our hospital from January 2011 to June 2012.Analyzed the sonographic features of the lesions,distribution of blood flow and blood hemodynamics of patients at baseline and compared these results with the pathological results after surgery.Results The diagnostic accuracy of breast cancer using combined two-dimensional ultrasound and color Doppler was 84％ (42/50).Among there four patients were missed diagnosis,three were diagnosed of fibroma and the other one was diagnosed of adenosis.Among the four misdiagnosed cases,one case was diagnosed of chronic inflammatory response,one case was with fibroma,one case had intraductal papilloma lesion apocrine metaplasia and the other one had nodular granuloma.Conclusion Ultrasound is a convenient,safe and painless checking method for breast cancer.The blood flow characteristics of two-dimensional ultrasound with color Doppler flow plays an important role in the diagnosis of breast cancer.

Objective: To observe the accuracy rate, false negative rate of B ultrasound examination in the diagnosis of endometrial polyps and discuss the diagnosis value. Methods:Selected 80 cases of patients with endometrial polyps treated in the hospital from 2013 January-2013 year in June as the research object and they were confirmed by pathological examination, mean age (43.9±11.2) years old, they were examined by B ultrasound examination before and after menstruation, then analyze sensitivity, specific degrees of B Ultrasound performance combining with pathological examination and the survey of satisfaction for patients. Results:the patients before and after the menstrual endometrium showed different degrees of thickening, ranging from 1.2-2.4 cm, the probe showed ligulate, papillary substance not uniform, single, multiple echo;after analysis 73 cases were diagnosed as endometrial polyp, the correct diagnostic rate was 91.2%and the false negative rate was 8.8%. 76 cases (95%) were satisfactory for the simplicity, low cost, accurate diagnosis. Conclusion:B ultrasound examination in the diagnosis of endometrial polyps has high correct rate and is noninvasive, safe, and is easily accepted by patients, so it has important clinical value.

Objective:To analyze the diagnostic value of colour Doppler ultrasonography and X-ray plain film radiography in upper urinary tract calculi.Methods: 97 cases of upper urinary tract calculi were randomly selected in our hospital between 2012 January and 2013 January, who were examined by colour Doppler ultrasound and X-ray film and then the results were analysed and compared.Results: In the 97 cases with colour Doppler ultrasound, 92 cases were the positive renal calculi, and the effective diagnosis rate was 94.8%. And then in the 97 cases with X-ray film, 67 cases were the positive renal calculi, and the effective diagnosis rate was 69.1%. The positive diagnosis rate in the group with colour Doppler ultrasound was higher than that in the group with X-ray film (x2=16.34,P<0.05). In the 97 cases with colour Doppler ultrasound, 52 cases were the positive ureteral calculi, and the effective diagnosis rate is 53.6%. And then in the 97 cases with X-ray film, 69 cases were the positive ureteral calculi and the effective diagnosis rate was 71.1%. The positive diagnosis rate in X-ray film group was higher than that in the group with colour Doppler ultrasound (x2=10.65, P<0.05).Conclusion: In diagnosis of upper urinary tract calculi, the diagnosis rate of renal calculi is higher by colour Doppler ultrasound and the diagnosis rate of ureteral calculi is higher by X-ray film. Both methods have advantages and disadvantages in inspection, and we should combine them in clinical practice.

Objective: To investigate the effects of ?-melanocyte-stimulating hormone(?-MSH) on TGF-?_1 secretion of fibroblasts from keloids so as to find a novel evidence to research etiology of keloids.Methods:Fibroblasts isolated from human keloids were cultured in DMEM medium with 15% bovine serum and treated with ?-MSH. The level of TGF-?_1 was measured with ELISA method. Results: ?-MSH at the concentration of 10 -6 mmol/L triggered the TGF-?_1 secretion of fibroblasts. Conclusion:?-MSH possesses the effect of triggering human keloid fibroblasts to secrete TGF-?_1, and its effective concentration is 10 -6mmol/L.

Objectives To sum up the experience of the gasless laparoscopic surgery using home-made abdominal-wall-take-up.Methods Using home-made abdominal-wall-take-up application on 15 patients,including 12 cholecystectmy,3 appendectomy.Every patients was used epidural block.Results All the surgery were successed and every patients had no complication.The hospitalization was 4～40 days (average 13.6 days),the time of operation was 50 to 215 minutes (average 89 minutes), fee of hospitalization was 5487 yuan.Conclusions It conclude that the gasless laparoscopic surgery using home-made abdominal-wall-take-up application is a safe,economic,useful method,which adapts to the situation of China.It reinforces the gas laparoscopic surgery.

Objective:To introduce a method combining thread-burying in eyebrow and construction of double eyelid for treatment of upper eyelid cutis laxa in the middle-aged and young.Methods: We used 3-0 non-invasive thread for intradermal suture and fixed the eyebrow to the superciliary periost,then double eyelid construction was performed to remove the superfluous skin of upper eyelid in 23 patients with upper eyelid cutis laxa.Results: All the 23 cases obtained satisfactory clinical outcomes, with the upper eyelid cutis laxa obviously improved.Conclusion: Thread-burying in eyebrow combined with double eyelid construction is a simple and effective strategy for treatment of middle-aged and young patients with upper eyelid cutis laxa.

Because the exact pathological mechanism underlying hypertrophic scars and keloids is unknown,there are a great number of treatment modalities, but it is unsatisfactory for the effect of treatment for the pathological scar.The vast array of treatment modalities for hypertrophic scars and keloids described by literature,whose treatmetn effects are varied and none of them is universally accepted in permanent hypertrophic scars and keloids ablation,are reviewed in this article in order to afford a reference for clinic medico.

Objective To explore the changes of rennin-angiotensin system in rats with focal brain ischemia-reperfusion injury and the effects of intervention and neuroprotective mechanisms with Irbsartan. Methods The male SD rats were randomly assigned to sham operated group, ischemia-reperfusion (IR) group and Irbsartan pretreatment group. The focal IR model was made by suture occlusion of right middle cerebral artery (MCAO). At 24 h and 72 h following onset of MCAO with reperfusion,the neurologic impairment function scores and the infarction volume were evaluated, the mRNA expression of angiotensinⅡ type 1 receptor (AT1R) and angiotensinⅡ type 2 receptor(AT2R)were detected by RT-PCR, and AngⅡ levels and Renin activity were examined by radioimmuno-assay. Results (1) Pretreatment with Irbsartan could significantly improve neurological outcome and reduced infarction size. (2) In bilateral cerebral cortex, hypothalamus, brain stem and peripheral blood leucocyte, the mRNA expressions of the AT1R and AT2R were significantly increased after either 24 h or 72 h of MCAO with reperfusion (all P

Objective To explore the surgical procedures and effects for recurrent scalp dermatofibrosarcoma protuberans (DFSP).Methods Retrospective review was conducted in 7 scalp recurrent DFSP cases in the past 3 years.Intraoperative frozen section analysis of surgical margins was performed.Wide local excision with margins of 3 cm or more was applied,and excision of the skull external lamina or the entire layer of the cranial bones was performed once the skulls under the tumors were invaded.Frozen section analysis was again performed on all the margins.Secondary wound was repaired with local skin flap or fascial pedicled flaps.Surgically removed tumor was paraffin-embedded for HE-staining and immunohistochemical analysis.Results All of 7 tumor specimens were proved recurrent DFSP with histologic findings of intraoperation or postoperation.7 patients were all performed with wide local excision with margins of 3 cm or more,also with skull excision (5 cases with excision of skull external lamina and 2 cases with excision of the entire layer of skull).Negative margins were confirmed with intraoperative frozen sections or postoperative paraffin-embedded sections analysis were confirmed.Immunohistochemical analysis demonstrated that Ki-67 was all positive and CD34 was partially positive in 7 cases.No tumor recurrence or metastasis had been observed in any of our patients after a median follow-up duration of 17 months (range 15-41).Conclusions Wide local excision together with the skull invaded by tumors completely is effective surgical treatments for recurrent scalp DFSP; Use of scalp transferring technique could improve wound repair after complete tumor excision.