BACKGROUND:As a new kind of bone repair material, NovaBone has a unique biological activity and good biocompatibility. It has a good binding role in the clinical repair of bone defects, so as to effectively promote the bonding of soft tissue and bone tissue.
OBJECTIVE: To explore the clinical effect of NovaBone in the repair of bone defects due to limb fractures.
METHODS: Sixty-seven patients with bone defects due to limb fractures were enroled, 37 males and 30 females, aged 17-81 years. Al the patients underwent NovaBone repair. Three days after repair, routine blood test, heart and kidney function, serum complement C3, complement C4, immunoglobulin G, immunoglobulin A, immunoglobulin M were detected; 12 months after repair, X-ray observation was performed for bone graft fusion effect.
RESULTS AND CONCLUSION:Al patients presented with stage I healing, and there was no incision infection, rejection or non-specific inflammation. Patients felt no discomfort or developed symptoms in the bone graft region. Laboratory indexes showed no abnormity. At 12 months after treatment, bony union was seen in al patients, and there was 1 case of nonunion, 1 case of possible nonunion, 2 cases of uncertain nonunion, 33 cases of possible union, 30 cases of strong union, with a bone fusion rate of 94%. These findings suggest that NovaBone materials for repair of bone defect due to limb fractures have good biocompatibility and obtain good clinical effect.

Objective To explore the relationship between prenatal diagnosis indications and fetal chromosomal aberrations , and the security of amniocentesis. Methods The amniotic fluid cells were sampled by amniocentesis and cultured in 572 high-risk pregnant women from January 2012 to August 2015. The chromosomal karyotypes were examined by G-banding. Results The success rate of the first amniotic fluid cells culture reached 99.83%. In all the 572 valid samples , there were 20 cases of chromosomal aberrations and the abnormal rate was 3.50%, including 17 of numeric aberrations and 3 of structural aberrations. There were 7 cases of chromosomal aberrations in all the 299 elderly parturient in high-risk indications and the abnormal rate was 2.34%, and there were 13 cases of chromosomal aberrations in all the 273 non-elderly parturient and the abnormal rate was 4.76%. Conclusions (1)It is necessary to further diagnose in pregnant women with high-risk factors , including high age , abnormal screening and ultrasonic findings , and history of abnormal gestation and birth. (2)The chromosomal karyotype examination of amniotic fluid cells in high-risk pregnant women is one of the effective prenatal diagnosis methods in high security and accuracy , with which it can reduce the incidence of birth defects and the burden of family and society , and improve the quality of the population.

BACKGROUND:Apoptin is a protein which is synthesized in vitro or expressed by genetic engineering, without toxic and transformation activity of normal cel s. Apoptin can specifical y induce the apoptosis of tumor cel s and provide the opportunity of inhibiting the growth of cancer.
OBJECTIVE:To construct a prokaryotic expression vector for apoptin, optimize the expression conditions, and detect the activity of the purified protein.
METHODS:The apoptin gene that had been constructed was cloned into prokaryotic expression vector pET-28b (+), which was transformed into E.coli host bacteria. Apoptin was induced by isopropyl-beta-D-thiogalactoside, and analyzed by polyacrylamide gel electrophoresis. The inhibition activity of apoptin on tumor cel s was detected.
RESULTS AND CONCLUSION:Apoptin gene was successful y cloned into pET-28b (+). Apoptin protein was induced to express in form of inclusion body by isopropyl-beta-D-thiogalactoside (0.5 mmol/L) at 26 ℃. And the expression of apoptin with relative molecular mass of about 15 000 was identified by polyacrylamide gel electrophoresis. The target protein was purified by denaturation-renaturation and affinity chromatography, which has pro-apoptotic effect on lung cancer cel s H460 and H1299. The prokaryotic expression vector pET-28b-apoptin is successful y constructed. The apoptin protein with bioactivity is obtained, which al ows further functional study of apoptin.

10.3969/j.issn.2095-4344.2013.23.013

Objective To explore the diagnostic value of contrast enhanced ultrasound (CEUS),elastography and their combined use in diagnosing non-mass-like lesions on breast.Methods From November 2015 to January 2017,79 nodules diagnosed by conventional ultrasound were prospectively enrolled and examined by CEUS and elastography before surgery.Multivariate logistic regression models were established for CEUS and CEUS combined with elastography to diagnose breast malignancy.The diagnostic performances of CEUS,elastography and their combined use were evaluated with the receiver operating characteristic (ROC) curve.Results The CEUS features significantly predicting malignancy were hyperenhancement,heterogeneous enhancement,visualization of penetrating vessels (P ＜ 0.01).Furthermore,the multivariate logistic regression for CEUS combined with elastography showed that heterogeneous enhancement,visualization of penetrating vessels and elasticity score were the independent predictors of breast malignancy.The area under the ROC curve of CEUS combined with elastography was (0.915 ± 0.223),which was higher than that of CEUS and elatography (0.851 ±0.029,0.790 ±0.038,P ＜0.05).Moreover,the sensitivity,and accuracy of CEUS combined with elastography were 89.1％,and 86.1％,respectively.The omission diagnostic rate of CEUS combined with elastography (8.9％) was reduced.The diagnostic accuracy of CEUS combined with elastography was significantly higher than that of CEUS and elastography.Conclusions The diagnostic value of CEUS combined with elastography was remarkably significant in the differential diagnosis of non-mass-like lesions on breast.