BACKGROUND:The effect of dimethyl sulfoxide cryoprotectants has been got a lot of verification in the low-temperature medical applications. But there is no literature addressing microdialysis detection of dimethyl sulfoxide cryoprotectants. OBJECTIVE:To investigate the microdialysis relative recovery of different concentrations of dimethyl sulfoxide cryoprotectants used for limb reattachment. METHODS:In vitro linear probe relative recovery of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) was detected by retrodialysis gain and loss method. The correction in vivo experiment was done to estimate dimethyl sulfoxide relative recovery in rabbit amputated limbs. RESULTS AND CONCLUSION:The relative recoveries of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) were (49.49±3.56)%, (46.30±1.48)%, (52.66±2.54)%using retrodialysis gain method and (50.99±6.89)%, (43.86±1.35)%, (50.67±0.75)%using retrodialysis loss method. The average recoveries were (49.48±3.18)%and (48.51±4.03)%, respectively. There was no difference in the relative recovery of dimethyl sulfoxide detected using two methods. The change of dimethyl sulfoxide concentration could not influence the retrodialysis gain and loss method calibration results. The recovery was (15.45±4.8)%in vivo. These findings indicate that the microdialysis technology is suitable for dimethyl sulfoxide sampling in vivo that has no obvious influence on the relative recovery.

Objective To study the AAAS gene mutations in a child with autosomal recessive Allgrove syndrome. Methods Clinical data were collected and blood samples were obtained from the proband of Allgrove syndrome and her parents. Genomic DNA was extracted and sequenced by PCR amplification. Subclone sequencing was performed to validate the gene mutations. The disease-causing potentials of mutation genes were evaluated by the Mutation Taster, and the target protein tertiary structure was modelled by the Swiss Model. Results A new heterozygous insertion mutation(c. 1347_1348insG) of exon 15 in the proband was identified and firstly reported. Other two reported mutations were detected, which were the heterozygous mutation c. 688C>T in the patient and her mother, and the homozygous mutation c. 855C>T in the proband and her parents. In addition, it was confirmed that the novel compound heterozygous mutations(c. 688C>T, c. 1347_1348insG) in the AAAS gene of the proband were pathogenic mutation locus. Conclusion The heterozygous mutation(c. 1347_1348insG) of AAAS gene was firstly reported. In case of the patients being clinically misdiagnosed, related-gene detection should be performed for the patients who were diagnosed with primary adrenal insufficiency during the period of infants and young childhood.

Objective To use thermoluminescense dosimeters (TLDs) to evaluate the radiation doses to various critical organs in the computerized imaging reference systems (CIRS) 5 years old pediatric anthropomorphic phantom result ing from Varian kilovohage cone beam CT (kV-CBCT) system based on the standard scanning protocols.Effective dose were also calculated based on dose measurement.Methods A batch of TLDs with consistency no larger than 2％ were selected and annealed.First,the doses in an anthropomorphic pelvic phantom were measured using a CT chamber and TLDs,respectively,based on the standard pelvic protocols.The ratio of the both measurements is the TLD conversion coefficient.Other TLDs from the same batch were placed between two tissue-equivalent inserts and placed into the pre-drilled organ cavities of the pediatric phantom.By using standard protocols,the organs dose were measured,based on which the corresponding effective doses were calculated.Results The TLD conversion coefficient was 3.91 mGy/per reading.By using the standard head,low-dose thorax,pelvis protocol,the whole body effective dose was 0.63,6.85 and 19.3 mSv,respectively.Conclusions It is feasible for using the CT chamber-calibrated TLDs to measure the radiation doses from kV-CBCT to pediatric anthropomorphic phantom.The effective dose in pelvic protocol was higher than in thorax and head protocol,indicating that the pelvic protocol has a penitential to lead to larger radiation damage and higher risk of secondary cancer.