Objective To investigate the impact on the endothelial cell function and renal blood flow through effective treatment for elderly patients with hypertension. Methods 64 cases of elderly hypertensive patients over 70years old with effective treatment, according to pre-treatment blood pressure, were divided into two groups: hyptension level-2 group(30cases) and hypertension level-3 group(34cases), and 30 cases of elderly people with health physical examination during the same period were setlected as the control group. Endothelial cell function was detected, including the endothelin-1 (ET-1)、 nitrous oxide (NO), thromboxane B2 (TXB2)、 6-keto-prostaglandinF1α (6-K-PDGF1α) in plasma. Renal blood flow was explored by color doppler ultrasonic instrument ,involving peak velocity in systole(PSV) and lowest velocity in end-diastole(EDV) of renal arteries, segmental arteries and interlobar arteries.After indicators had reached the standard 1 month in the hypertension level-2 and hypertension leve1-3 patients with effective antihypertensive therapy, the difference of ET-1, NO, TXB2,6-k-PGF1o and PSV, EDV among the three groups were compared. Results The differences were statistically significant in ET-1, NO, TXB2,6-k-PGF1α, PSV and EDV before treatment among three groups (P ＜ 0.05). After blood pressure treated had reached the standard 1 month,indicators were no statistically meaningful difference among the three groups (P ＞ 0.05). Conclusion After effective clinical treatments, the endothelial cell function and renal blood flow of the elderly hypertensive patients can be improved. At the same time, to delay renal damage in elderly patients with hypertension provide a theoretical basis.

Objective:To study the effects of the modified sandblasting surface treatment on the bone implant interfacial shear strength of titanium dental implant.Methods:Titanium implants with smooth surface or modified sandblasted surface were inserted into femoral medialis condyli of dogs at random.The samples of implant with surrounding bone tissue were prepared in blockes and the shear strength of the interface was measured with Instron electronic pull out tester 2,4 and 12 weeks after operation. The modified sandblasted surface of the implant was observed with SEM and X ray spectroscopy 12 weeks after operation.Results:2 and 12 weeks after implantation the shear strength(MPa) between bone tissue and implants with modified sandblasted surface was 4.28?0.88 and 7.54?0.34 respectively,that between bone tissue and implants with smooth surface 0.09?0.08 and 1.43?0.46 respectively.SEM and X ray spectroscopy observation revealed that the most parts of the sandblasted surface of the implants were covered by bone tissue and bone matrix deposited into the pores created by the acid etching.Conclusion:The rough surface of titanium dental implant created by the modified sandblasting surface treatment can enhance the shear strength between bone tissue and implant.

BACKGROUND: The nomination of undifferentiated spondyloarthropathy (USpA) has provided clues for the early diagnosis of spondylitis ankylopoietica and other subsets of SpA, thereby avoiding possible wrong treatments.OBJECTIVE: To investigate the prevalence of SpA in the workers and their adult relatives aged over 16 years old from a factory in Beijing urban district, as well as the results of human major histocompatibility complex (MHC) B27 and X-ray inspection on sacroiliac joint.DESIGN: Systematic sampling investigation.SETTING: Department of Internal Medicine, Chenghai District People's Hospital of Shantou City; Rheumatism Research Institute, Medical College of Shantou University; Department of Internal Medicine, Beijing Chaoyang Hospital.PARTICIPANTS: A survey was conducted among 1982 workers and their adult relatives aged over 16 years from a factory in Beijing urban district between May and October 1999; there were 1,025 males and 957 females.They all volunteered into this study after informed of the experiment.METHODS: All examinees were surveyed door to door with the modified Chinese-International Anti-rheumatic Alliance Rheumatism Questionnaire,those with positive results were subjected to physical examination and those suspicious cases were subjected to MHC-B27 and X-ray examination.MAIN OUTCOME MEASURES: The prevalence of SpA in the workersand their adult relatives aged over 16 years from a factory in Beijing urban district, as well as the results of MHC-B27 and sacroiliac joint X-ray inspection.RESULTS: Totally 1,982 subjects were enrolled in this study and all entered the result analysis. The prevalence of spondyloarthropathy was 1.61%(32/1 982), amongst which the prevalence of ankylopoietic spondylitis,USpA, and psoriastic arthritis was 0.31% (6/1 982), 1.21% (24/1 982),and 0.10% (2/1 982), respectively. No other subsets of SpA were observed. USpA was mainly presented by inflammatory lumbago and asymmetric arthrosteitis [83% (20/24), 62% (15/24)], while rheumatoid factor was negative in all subjects. Meanwhile, MHC-B27 was proved positive in 25% (6/24) subjects with the male-to-female ratio of 1.2:1.CONCLUSION: The prevalence of SpA is 1.61% in Beijing urban area,and SpA mainly consists of ankylopoietic spondylitis and USpA.

Objective:To investigate the effects of Liuweidihuang pill on the insulin levels in sera and pancreatic islets from spontaneous mouse models of human type 2 diabetes administrated with different doses .Methods:The 6-8 week-old KK-Ay mice were randomly divided into three groups including no drug control group ,low-dose group and high-dose group,in addition C57BL/6J mice were used as a genetic control group .All the animals were given with different dose Liuweidihuang pill solutions or sterile distilled water by intragastrical administration for fifteen weeks .The fasting blood glucose ,body mass and food consumption were measured weekly .The serum insulin levels were surveyed by ELISA .And the insulin levels in the pancreas islets were detected by immunofluorescence and immunohistochemistry .Results:Decreased fasting blood glucose ,controlled body mass and food consumption ,and lower levels of insulin in the sera and pancreas islets were confirmed from the KK-Ay mice administered with Liuweidihuang pill .Furthermore,the low dose program exhibits a stronger effect .Conclusion:Liuweidihuang pill has exhibited relatively therapeutic effects in the spontaneous type 2 diabetes mice including controls of hyperglycemia and body mass and relieving insulin resistance .In addition , the low-dose regimen showed even better treatment in controlling insulin levels in the sera and pancreas islets .

Objective To prepare a recombinant pneumococcal surface protein A clade 4 ( PspA4) and to analyze its immunogenicity.Methods The gene encoding PspA4 protein was synthesized and inserted into pET-20b to construct the recombinant expression plasmid.The transformed E.coli strains carrying expression plasmid were induced to express PspA4 protein.ELISA was performed to analyze the ti-ters of PspA4-specific IgG in a mouse model.Results The recombinant PspA4 protein of high purity ( 90%) was successfully prepared.The titers of PspA4-specific antibody in mice received PspA4 immuniza-tion were 106 times higher than those of the blank control group, suggesting that the expressed PspA4 protein had the advantage of high immunogenicity.Conclusion This study suggested that the PspA4 protein might be used as one of the candidate protein for the development of pneumovax and laid a foundation for further in-vestigation on pneumococcal protein based vaccine.

Objective To explore the correlation between acute myocardial infarction(AMI)and neutrophil extracellular traps-os-is(NETosis)in the regulation of cardiomyocyte autophagy in mice.Methods The mouse model of AMI was established in C57BL/6mice by ligation of the left anterior descending coronary artery.Mouse primary cardiomyocytes were treated with oxygen glucose deprivation(OGD)to build an in vitro model of cardiomyocyte injury.Neutrophils were treated with PMA(NETosis inducer)/DNas Ⅰ(NETosis in-hibitor),the supernatant was taken to treat OGD-induced cardiomyocytes,and rapamycin(Rap)was used to treat OGD-induced car-diomyocytes.The myocardial infarction area was detected by TTC staining;serum cTnI level was detected by enzyme-linked immunoad-sordent assay(ELISA);cardiomyocytes apoptosis was detected by TUNEL staining;NETosis marker levels in neutrophil supernatant were detected by ELISA,and related protein expression levels were detected by Western blot.Results TTC staining showed that compared with the sham-operated group,the myocardial infarction area of the mice in the model group was significantly increased,the level of cTnI in serum was significantly increased(P<0.05),the apoptosis of cardiomyocytes was increased,and the levels of Beclin-1 and LC3-Ⅱ/LC3-Ⅰ were significantly increased,the p62 protein expression level was significantly decreased.Compared with the control group,the expression levels of apoptosis and cleaved caspase-3 were significantly increased under OGD conditions(P<0.05).Compared with the control group,the levels of NETosis markers MPO-DNA,MPO,and NE in the neutrophil supernatant in the PMA group were signifi-cantly increased;compared with the PMA-treated group the apoptosis level of cardiomyocytes in the PMA + DNas Ⅰ group was signifi-cantly decreased;the levels of Beclin-1 and LC3-Ⅱ/LC3-Ⅰwere significantly decreased,and the protein expression level of p62 was significantly increased;compared with the PMA + DNas Ⅰ group,Rap treatment could enhance the levels of Beclin-1 and LC3-Ⅱ/LC3-Ⅰ,and inhibit the level of p62(P<0.05).It also significantly reversed the decrease in apoptosis induced by PMA + DNas Ⅰ.Conclusion Neutrophil NETosis can promote AMI in mice by regulating cardiomyocyte autophagy,which can provide a new direction and theoretical basis for the research and development of therapeutic drugs for AMI.

Objective To investigate the efficacy of real-time visual feedback on improving the quality of manual chest compression in ambulance.Methods Ten pre-hospital doctors with cardiopulmonary resuscitation experience,aged under 40 years,were recruited to this randomized,crossover,manikin research and randomly assigned into control group (n=5) and feedback group (n=5) by the sealed envelope method.The setting place was a moving ambulance with the velocity of 25～50 km/ h.The whole process consisted of two sessions.In control group,which received feedback in the second session,chest compressions were performed without interruption during each of the three 2 min phases per session,resting for 2 min between phases and for 5 min between sessions.In feedback group,which received feedback in the first session,chest compressions were performed without interruption during each of the three 2 min phases per session,resting for 2 min between phases and for 5 min between sessions.Data of compression rate,compression depth,compression detention and compression accuracy rate were collected.Results In control group,the compressions rate was lower and compression detention was shorter during the second session compared with those during the first session [(109.8±±4.7) r/min vs.(121.2± 10.1) r/min,(6.5±2.1) r/min vs.(10.4±2.8) r/min,all P＜0.05],while the compression accuracy rate during the second session was higher than that during the first session [(28.2±±14.3) ％ vs.(16.8±9.9) ％,P＜0.05].There was no significant difference in compression rate between the two sessions in control group;Compression frequency,compression depth,compression detention and compression accuracy rate did not significantly change between the two sessions in feedback group (all P＞0.05).In the whole process,the compression rate was lower and compression detention was shorter in the feedback group compared with the control group [(111.1±5.1) r/min vs.(115.5±9.7) r/min,(6.5±1.8) vs.(8.4±4.6) r/min,all P＜0.05],and the compression accuracy rate in the feedback group was higher than that in the control group[(22.5±13.4) ％ vs.(26.7±16) ％,P＜0.05].There was no significant difference in compression rate between the two groups during whole process (P＞0.05).Conclusions Although real-time visual feed back improved the quality of manual chest compression in ambulances,which demonstrated more reasonable compression rate,less compression detention and higher compression accuracy,the overall quality of reuscitation was still not enough to achieve effective treatment.This implies that more optimal methods are required to transfer the patients suffering cardiac arrest.

OBJECTIVETo evaluate the prevalence of primary gout in the Shantou area, China, and to understand its clinical features.

METHODSSamples from three surveys of the Chenghai across ten years were studied. Clinical, laboratory and radiology data of 419 cases of primary gout were collected and analyzed.

RESULTSPrevalence of primary gout in the three surveys were 0.17%, 0.15% and 0.26%, respectively. Among the clinical patients, the ratio of male to female was 21:1; the average age of onset of women was significantly higher than that of men. The number of cases in the last ten years has been increasing. In the first wave of cases, the first metatarsophalangeal joint was involved in 82.1% of all cases; no fourth metatarsophalangeal joint involvement had been observed. Hyperlipemia was the most common accompanying abnormal laboratory test (57.1%). Radiographic bone erosion was found in 49.4% patients, most of them with disease duration more than 5 years.

CONCLUSIONSThe prevalence of primary gout in Shantou area has been increasing in the last ten years. Changes in diet and lifestyle may be responsible for this rapid increase.

Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Gout ; complications ; diagnostic imaging ; epidemiology ; Humans ; Male ; Middle Aged ; Radiography