Objective To manifest the angiogenic effect and heart function improvement of transplantation therapy through the experiments of porcines. Methods The coronary arteries of porcines were ligated to create myocardial infarction and then the bone marrow mononuclear cells were injected into the coronary artery. Three weeks after transplantation the heart function was measured by echocardiography and left ventricular angiography. The myocardial blood perfusion was measured by single photon emission computerized tomography. The number of microvessels was also counted. Results After the injection of bone marrow mononuclear cells, left ventricular angiography showed the increasing of dP/dt. The myocardial blood perfusion measured by single photon emission computerized tomography increased. Collateral vessels occurred, and the number of vessels was higher than that of the control (56.6?11.7/mm2 vs. 37.2?8.4/mm2,P

Objective To evaluate the effect of percutaneous stenting for renal artery stenosis. Methods Stent implantation was conducted in 27 patients(Interventional Group) with renal artery stenosis from January 1997 to December 2004 in this hospital.Patients' blood pressure,renal functions,and quality of life were recorded and compared with another group of 27 patients receiving medical treatment(Medical Group) during this time. Results The procedure of renal artery stenting was superior to the medical treatment in antihypertensive effect,renal function improvement,raise of life quality,and increase of survival rate.The procedure offered a high success rate(88.9%,24/27) and low re-stenosis rate(12.5%,3/24) and an incidence of complications(18.5%,5/27).The Interventional Group was remarkably superior to the Medical Group with respect to the decrease of blood pressure and creatinine and the increase of glomerular filtration rate,with significant differences.Follow-ups for 6 months ~ 8 years and 6 months(median,1 year and 9 months) revealed that normal daily activities and works were achieved in 19 patients in the Interventional Group and in 12 patients in the Medical Group. Conclusions Percutaneous renal artery stenting in the treatment of renal artery stenosis is effective.

Objective The purpose of the study is to analyse the counseling contents in psychological hot line during four years and the characteristics in different groups of the people. Methods Inputting all the completed recorders in hot line from January, 1995 to December, 1999, and using the Statistic Analysis System to analyse the data. Results The main age groups of the counseling people are 20～years and 30～years (77.39%), and 87.43 percent of the people has good education. The main counseling contents are related to mental disorders (23.61%), emotional difficulty (19.74%), love (17.07%), social intercourse (9.69%), marriage (9.15%), consulting for other persons (7.67%), sexual problems counseling (6.36%), and others. Conclusions The main groups of people in hot line are young and good educated people. The counseling contents have touched upon many areas, but mental disorder is the most numerous problems. The main problems in different groups of people are marked difference.

Objective The 47 myocardial infarction patients (male 41, female 6, average ages 55 7?9 7 years) with silent myocardial ischemia (type Ⅱ SMI) were chosen The relationship was studied between the history of myocardial infarction (MI)and the degree of coronary arterial stenosis and the residual degree of stenosis after percataneous transluminal coronary angioplasty (PTCA) Methods All patients with MI were performed with exercise testing electrocardiogram to investigate the degree of myocardial ischemia The patients were also studied the degree of coronary arterial stenosis before and after PTCA Results The results showed before PTCA, the degree of the patients′ coronary arterial stenosis with a period of no more than three months of MI was higher than that of those patients′ with a period of over three months( P

In order to explore the mechanism of cardiac damage by PTH, the release of PGI2 from heart cells was measured. The results showed that bPTH ( 1-34) increased the release of 6-keto-PGFla from heart cells in a dose dependent manner. Calcium inophone A23187 also increased the 6-keto-PGF1? release, while EDTA and verapamil reduced it. These suggest that the PGI2 synthesis in heart cells was affected by intra and extracellular calcium. The significance of bPTH( 1-34) induced increase of PGI2 synthesis might be associated with interference of energy metabolism, and then, cell damage.

Objective: To observe the effects of sodium heparin and low molecular weight heparin on the release of plasma hepatocyte growth factor (HGF) in senior coronary heart disease patients.Methods: Fifty-four senior patients with coronary heart disease were divided into three groups: intravenous sodium heparin, subcutaneous sodium heparin, and subcutaneous low molecular weight heparin (LMWH). Plasma HGF and vascular endothelial growth factor (VEGF) were measured before and after injection.Results: Plasma HGF was increased rapidly and significantly after intravenous injection of sodium heparin, reaching its peak level (about 48 fold) after approximately 10 minutes. Plasma HGF was also increased rapidly and significantly after subcutaneous injection of sodium heparin and LMWH, reaching its peak level (about 4 and 5 fold in sodium heparin and LMWH respectively) after approximately 2-3 hours. Conclusion: The rise of plasma HGF after heparin treatment suggests that heparin has some other biological effects in addition to its anticoagulant property through HGF. By this mechanism, the administration of heparin may be of some importance in the reparation of cardio-vascular diseases.

ObjectiveTo evaluate the curative effect of supplementedGanmai-Dazaodecoction for malignant tumor correlation with depression.MethodsA total of 98 patients with malignant tumor-related depression were divided into a treatment group and acontrol group according to the random number table. The control group were treated with flupentixol and melitracen tablets (Deanxit) combined with conventional treatment, the treatment group were treated with oral administration of traditional Chinese medicine supplemented Ganmai-Dazaodecoction combined with conventional treatment for 8 weeks. The Hamilton Depression Scale (HAMD) was used to evaluate the curative effect.ResultsThe effective rate showed no significant difference between the treatment groupand the control group (79.59%vs.75.51%;χ2=0.059,P=0.809). HAMD scores in both groups were significantly decreased after the treatment than before treatment (12.8±3.4 vs. 29.4±4.7 in the treatment group, 12.3±3.6vs.28.7±4.5 in the controlgroup, allP<0.05), there was no significant difference in HAMD scores after the treatment between the treatment group and the control group (t=0.707,P=0.481). ConclusionCurative effect of supplementedGanmai-Dazaodecoction is equal to flupentixol andmelitracen tablets in patients with malignant tumor-related depression.

Objective To explore the status of job burnout of medical staffs in minority border areas. Methods A total of 906 medical personnel from Pu'er hospitals in Yunnan Province were as-sessed with Maslach Burnout Inventory General Survey (MBI-GS) by t test, descriptive statistics, chi-square test, ANOVA and LSD-t pairwise comparison. Results ①The emotional exhaustion, cynicism and reduced personal accomplishment of medical staffs in minority border areas (1.55±1.40, 0.99± 1.29, 2.47±1.98) are significantly lower than general areas (P=0.000, 0.010, 0.000); ②emotional ex-haustion, cynicism and Maslach Burnout Inventory (MBI) total points of different levels of hospital medi-cal staff, the score of which in descending order are:municipal, county and township (town) levels (P=0.000, 0.000, 0.000); ③Female medical personnel's emotional exhaustion and cynicism are scored significantly higher than males medical staff (P=0.000, 0.025); ④The scores of emotional exhaustion, cynicism, reduced personal accomplishment and MBI total points of Bachelor medical staff are signifi-cantly higher than those of colleges as well as secondary schools and below (P=0.000, 0.000, 0.000, 0.012); ⑤The scores of reduced personal achievement of Lahu medical staff are higher than those of other ethnic groups (P=0.009). Conclusion In medical staffs in minority border areas there exists a certain extent of job burnout, which presents differences among genders, educational background, hos-pitals and ethnic groups.

Objective:To explore the related factors of ventricular arrhythmia (VA) complicating acute phase of myocardial infarction and their effects on short term prognosis. Methods:A total of 161 subjects with acute myocardial infarction (AMI) were divided into 5 groups according to VA types: frequent single ventricular premature beat group(n=10),bigeminy or paired ventricular premature beat group (n=21),non-sus- tained ventricular tachycardia group (n=31),ventricular tachycardia and fibrillation group (n=11) and control group (n=88). The characteristics of coronary artery and left ventricular ejection fraction were determined. Results:The incidence of left main coronary occlusion was more frequent in ventricular tachycardia and fibrillation group than in control group (P

A method was developed for the determination of zeranols (α,β-zearalanol,α,β-zearalenol,zear-alanone,zearalenone) with RRLC-MS/MS in the plant tissue. The samples were extracted with acetonitrile and reextracted with aqueous alkaline solution,cleaned up with MAX column and then determined by RRLC-MS/MS using multiple reaction monitoring ( MRM) scan mode. The results showed that the working curves were linear in the range of 0 -20 μg/kg. The limits of detection ( LOD) of zeranols were 0.5 μg/kg and the limits of quantification (LOQ) was 1. 0μg/kg. Extraction recoveries for zeranols ranged from 75. 8% to 105.4% and RSD was between 2.4% and 12.1%. The method is suitable for the determination of zeranols in the plant tissue.