Objective To analyze the association between serum uric acid (SUA)level and diabetic peripheral neuropathy(DPN)in the elderly patients with type 2 diabetes mellitus(T2DM).Methods A total of 1 091 cases of elderly T2DM in-and out-patients in our hospital from January 2012 to December 2015 were enrolled in this study.The patients with DPN was diagnosed by neuropathy disability score.All the subjects were divided into two groups according to whether the patients with DPN:NDPN(n=574)and DPN group(n=517).The differences in clinical manifestations and biochemical parameters were compared between the two groups,meanwhile the mobidity rates of hyperuricemia between the two groups were calculated.Logistic regression analysis was used to analyze odds ratios between SUA and the prevalence of DPN.Results The mean serum uric acid of DPN group was(338.79±93.76)μmol/L versus NDPN group's(301.82± 58.94)μmol/L(P＜0.05).The prevalence of hyperuricemia was 20.12％ in DPN group,higher than that in NDPN group(13.24％)(x2 =15.962,P =0.000).Compared with NDPN group,age,duration of diabetes,history of cerebral infarction and the levels of SUA,HbA1C and TG in DPN group were significantly increased,and the levels of eGFR and TBIL decreased(P＜0.05),and there were no significant differences in the other indexes between the two groups (P＞ 0.05).Logistic regression analysis showed that increased SUA level was an independent risk factor for DPN,OR =2.293 (95 ％ CI:1.497-3.824) (P ＜ 0.05).Conclusions It has closely correlation between SUA level and DPN,and SUA is one of the independent impact factors for DPN in the elderly patients with type 2 diabetes mellitus.

Objective To investigate the risk factors for hip fracture in elderly people in Ningbo,in order to provide the basis for prevention and treatment of hip fracture.Methods A case-control study were conducted in study on 450 cases of hip fracture patients aged over 60 years in Ningbo and 450 subjects of control as 1 ∶ 1 matched case control study.A univariate analysis of hip fracture was performed by x2 test.Risk factors for hip fracture were analyzed by logistic regression.Results Poor self-care ability (OR=5.05),light manual labor occupation (OR=4.10),history of cerebrovascular events (OR=2.59),smoking (OR=2.14) were the risk factors for hip fracture in elderly men,while poor self-care ability (OR=11.36),light manual labor occupation (OR=2.41),osteoporosis (OR=3.19),early menopause (OR=6.89),low body mass index (OR=5.05) were the risk factors for hip fracture in elderly women.The protective factors for hip fracture was milk uptake (OR=0.29) Conclusions Early interventions for hip fracture risk factors,and prevention and treatment of osteoporosis can help reduce the occurrence of hip fracture.

The present study reported the effect of two male antifertility agents gossypol acetic acid and GTW on DNA of C3H10T1/2 mouse fibroblasts. Our results showed that the cells treated with gossypol or GTW at high concentration (2-3 ?g/ml) for 4 hours, show silver grains in their nuclei as much as the positive control group, N-methyl N′-nitro-N-nitrosoguanidine (MNNG) a known carcinogen. However, if the agents were used at moderate concentrations (0.5-1?g/ml), the silver grains were much less, if the concentrations of gossypol or GTW were of 0.1-0.3 ?g/ml, the silver grains were as less as the control group. In a colony-forming test, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration; while at moderate or low concentrations, the colony-forming rate was as high as 8.1-10.5％. Taking all of these results into consideration, we suggest that high concentrations of gossypol or GTW can damage cell DNA severely, moderate concentration of the agents break cell DNA to a certain extent, but the cells can repair, while low concentration of gossypol or GTW exert no obvious effect on cells. The significance of these observations was briefly discussed.

The contents of gallic acid, catechin, albiflorin, paeoniflorin, benzoic acid and paeonol extracted in different growth years, collecting season and of different parts of Paeonia lactiflora were determined. The results showed that the contents of catechin and paeoniflorin in Paeonia lactiflora collected in autumn are the highest, and the contents of benzoic acid was lower than that of those collected at other time. The longer is the age of Paeonia lactiflora, the higher is the contents of catechin and paeoniflorin. The contents of catechin and paeoniflorin in the root of Paeonia lactiflora were higher than those in other parts of the plant. There is a certain content of paeoniflorin in the leaves of Paeonia lactiflora. Judging from the result, paeoniflorin is synthesized in the leaf and then transported to the root. Catechin is not synthesized in the leaf, but mainly in the root. Paeonia lactiflora should be collected in autumn, and immature plant should not be collected.

OBJECTIVE To study characteristics and drug resistance of the pathogens of hospital acquired pneumonia(HAP)in cadre ward.METHODS Retrospectively analyzed the commonly encountered pathogens and their antibiotic resistance of HAP in cadre ward from Jan 2005 to Dec 2007.RESULTS A total of 174 strains bacteria were isolated.The top five pathogens were Pseudomonas aeruginosa(17.82%),Klebsiella pneumoniae(14.94%),Staphylococcus aureus(13.22%),Acinetobacter baumannii(9.20%)and Candida albicans(8.62%).Both Gram-negative bacteria and Gram-positive cocci were multiple drug-resistant.CONCLUSIONS The incidence of HAP in cadre ward is high and Gram-negative bacteria are the main pathogens.The strains isolated are multiple drug-resistant.It is important to study the characteristics and drug resistance of the pathogens.

Renal ischemia reperfusion injury refers to the recovery failure of renal function induced by renal perfusion after ischemia,and resulting in a series of pathophysiological reactions.At present,there are no sovereign drugs for the treatment of renal ischemia reperfusion injury.Based on the pathophysiological characteristics of renal ischemia reperfusion injury and the latest research results at home and abroad,the article reviewed the research progress in the therapeutical drugs for renal ischemia reperfusion injury,including apoptotic protease inhibitors,P-selectin antagonists and antioxidants,which could provide reference for the effective intervention with the disease.

Objective To explore the relationship between plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) level and the location and prognosis of acute ST-segment elevation myocardial infarction (STEMI) in elderly people.Methods From May 2012 to April 2014, the 137 elderly patients (aged≥60 years) with acute STEMI within 24 h after symptoms onset admitted in cardiovascular department of Ningbo First Hospital were selected as acute myocardial infarction group (AMI group), and 40 elderly healthy people (age≥60 years) as control group.The plasma NT-proBNP levels were detected by enzyme linked immunosorbent assay(ELISA), and compared between the two groups.The patients in AMI group were classified into four subgroups: anterior AMI group (A group, n=44), anterolateral AMI group (B group, n=21), anterior septal AMI group (C group,n=18) and inferior AMI group (D group, n=54), and the changes of plasma NT-proBNP levels in different infarction location were explored.Meanwhile, all patients were followed up for 30 days and 1 year to observe major adverse cardiac events (MACEs) and mortality rate, then to explore the relationship between plasma NT-proBNP level and prognosis in elderly people with STEMI.Results The plasma NT-proBNP levels were significantly higher in AMI group than in control group [(1 406.2±1 322.5)ng/L vs.(63.7±18.5) ng/L, P＜0.01].The changes of plasma NT-proBNP levels in high-to-low sequence were from A group to B group to C group to D group and to control group (P＜0.05).At 30 days, the plasma NT-proBNP levels were higher in death group than in survival group (P＜0.05), and were higher in MACE group than in non-MACE group (P＜0.05).At one year, there were no significant differences in plasma NT-proBNP levels between death group and survival group (P＞0.05), and the plasma NT-proBNP levels were higher in MACE group than in non-MACE group (P＜0.05).Conclusions In elderly STEMI patients, there is a correlation between plasma NT-proBNP levels and infarct location, with the highest NT-proBNP level in anterior AMI.The plasma NT-proBNP level could predict MACE.

Objective To investigate the mechanism,injury characteristics,and early interventions of bilateral femoral shaft fracture.Methods Twenty-five cases of bilateral femoral shaft fracture treated between December 2009 and June 2012 were enrolled in the study.Eighteen patients were males and 7 were females with mean age of 31.2 years (range,17-40 years).Nineteen cases were injured due to traffic accidents and 6 due to the hit by heavy objects.There were 47 sides with closed fracture and 3 sides with open fracture.Intramedullary nailing was performed at 32 sides and plate fixation was used at 18 sides.ISS,blood routine,some blood biochemical items,blood or plasma use,and hospitalization days were measured.Results ISS was (14.6 ± 5.2) points.Preoperative readiness time and hospital stay were (10.1 ± 3.7) days and (28.9 ± 8.4) days respectively.After hospitalization,measured values were (3.0 ± 0.4) × l012/L for red blood cells,(86.0 ± 13.5) g/L for hemogIobin,(47.0 ± 10.6) g/L for total protein,(31.4 ± 6.5) g/L for albumin,(124.1 ± 95.1) U/L for aspartate aminotransferase,(114.2 ± 107.1) U/L for alanine aminotransferase,and (1 848.7 ± 1 044.8) U/L for creatine kinase with significant differences from the reference ranges (P ＜ 0.05 or 0.01).Amount of blood transfused for 23 cases was (1 444.4 t 726.5) ml and plasma transfused for 19 cases was (305.6 ± 98.3) ml before operation.Volume of intra-and post-operative blood transfusion was (2 005.7 ± 1 153.1) ml.Conclusions Stabilization of vital signs predominates the early treatment of bilateral femoral shaft fracture.After the condition is stable,intramedulary nailing or plating can be performed concurrently.

Objective To evaluate the impact of play therapy to the pain and the compliance of hospitalized children during tht course of lumbar puncture operation.Methods Divided 60 children with lumbar puncture operation into the intervention group (30 cases) and the control group(30cases)randomly.Routine cares were used in the control group,while the play therapy was used in the intervention group in addition during the course of lumbar puncture operation.Results There were statistical significance in the comparisons of the changes of respiratory rate and pulse rate during the preoperative and intraoperative of lumbar puncture between the two groups,P＜0.01.There were also significant differences in the comparisons of subjective and objective pain score as well as the puncture compliance in the lumbar puncture operation.The score of FLAFF scale and the Wong-Baker scale in the intervention group was (2.52 ± 1.37) and (3.59 ± 1.15) respectively,which was significant lower than that of in the control group,(4.22 ± 1.97) and (4.93 ± 1.71),the difference was significant,P＜0.01.Conclusion Play therapy can relieve the pain of children with lumbar puncture operation,reduce the changes of the vital signs,promote the puncture compliance and shorten the time of the whole operation completeness.

Objective: To summarize surgery experience and efficiency of laparoscopic excision of choledochal cyst and reconstruction of biliary tract and to analyze the treatments administered during intra and post-operation of the biliary reconstruction. Methods: We enrolled 48 cases of video-guided laparoscopic intraoperative cholangiography, cholecystectomy, choledochocele resection, Roux-en-Y hepaticojejunostomy with an anti-reflux valve from August, 2001 to October, 2003.Their operative procedures and aims were retrospectively analyzed. Results: Forty-four out of 48 patients successfully underwent laparoscopy and recovered soon. Only 4 cases were transformed to open operation. The post-operation complications were found in 4 cases. Conclusion: Video-guided laparoscopic excision of choledochal cyst, biliary tract reconstruction and Roux-en-Y hepaticojejunostomy are an effective method of treatment with advantages of minimal injury, less bleeding, and sooner recovery.