Adenosine Triphosphate ; Mouth ; metabolism ; Receptors, Purinergic P2X ; metabolism

Child ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; surgery

BACKGROUND: It is thought in traditional Chinese medicine that "the head is the residence of intelligence", "the head is the leader of body, and the focus of the spirit". Therefore, scalp electro-acupuncture can induce resuscitation, relieve mental confusion, dredge the meridian and eliminate the depression in treatment of post-stroke depression.OBJECTIVE: To observe the curative effect of head acupuncture on poststroke depression.DESIGN: Randomized controlled and single blind evaluationSETTING: Department of Acupuncture and Moxibustion, Nanfang Hospital of Southern Medical University; College of Acupuncture and Massage,Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 90 patients with post-stroke depression were from Affiliated Nanfang Hospital and,Zhujiang Hospital of Southern Medical University, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were recruited. The Score of all the patients in Hamilton Rating Scale for Depression (HRSD) were ＞ 20. Ninety patients were randomly divided as scalp acupuncture group (n=46) and routine acupuncture group (n=44).METHODS: The patients in the routine acupuncture group were treated mainly at points of three-yang meridians of foot and hand at the hemiplegia side. The points include jianyu (LI-15) , binao (LI-14) , quchi (LI-11),shousanli (LI-10), waiguan (SJ-5), hegu (LI-4), biguan (St-31), liangqiu (St-34), zusanli (St-36), yanglingquan (GB-34), kunlun (UB-60), jiexi (St41). The above points were divided into 2 groups, 1 group each day, alternately. Even reinforcing-reducing method was used, with 30-minute needle retention, 6 successive days treatment with 1 day rest, for 6 weeks totally.The patients in the scalp acupuncture were treated with, besides the above method, scalp acupuncture. Mid-parietal line, mid-frontal line, laterofrontal lineI (both sides)were chosen. After arrival of qi, G6805 type electro-acupuncture apparatus (interrupted wave, 50 Hz,4 V) was connected, with 30-minute needle retention, 6 suocessive days treatment with 1 day rest, 6 for 6 weeks totally. HRSD was used to evaluate the status of depression before and after treatment. Stroke score was used to evaluate the curative effect of stroke.MAIN OUTCOME MEASURES: HRSD scores and curative effect of apoplexy before and after treatment of the patients in two groups.RESULTS: According to the intention treatment, all 90 patients entered the result analysis. ① HRSD score after treatment was lower than that be fore treatment in the scalp acupuncture group,and that after treatment group in the routine acupuncture group (8.55±1.73,30.61±4.02,15.04±2.33,P ＜ 0.01 ). ②There was no significant difference of the total effective rate in the two groups , but some tendency indicated the effective rate in the scalp acupuncture group was better than that in the routine acupuncture group ( 100% ,98% ,x2=1.9167 ,P ＞ 0.05).CONCLUSION: Scalp electro-acupuncture has obvious curative effect on post-stroke depression. The amelioration of melancholia has a certain promoting effect on rehabilitation of apoplexy.

miRNA is a kind of endogenous non-coding short RNA. Mature miRNA was formed through the process of shearing and transporting after genetic transcription. miRNA exhibits many important biological functions through regulating expression and translation of target mRNAs. Different miRNAs may act as oncogenes or antioncogenes, and have tissue specificity. The progress of the tumorigenesis is usually accompanied by expression-profile changes of miRNAs. MiRNA regulates many tumor biological behaviors such as differentiation, proliferation, apoptosis, invasion, metastasis, and drug resistance of tumors. Furthermore, some miRNAs have clinical significance in predicting prognosis of tumor patients.

Objective To investigate the changes of T cells and disease development in the mice with Smad3 deficiency in the bone marrow cells.Methods Bone marrow cells obtained from Smad3 null(Smad3~(-/-))mice and wild type(Smad3~(+/+))mice were injected to Co~(60)-irradiated GFP mice,respectively.The general states of the bone marrow recipients were observed.The mice were sacrificed at the sixth week and the histopathological changes in the intestines were examined.The changes of T cells from lymph nodes were detected by flow cytometry.Results Smad3~(-/-) bone marrow recipient mice appeared a wasting syndrome and intestinal inflammation.The amount of CD4~+ CD62L~(lo) T cells in lymph nodes was significantly increased.Conclusion These results indicate that the mice with Smad3 deficiency in the bone marrow cells present an inflammatory disorder and their T cells are activated.

Objective To analyze the clinical and pathologieal characteristics of IgA nephmlogy secondary to primary sj(o)gren's syndrome(pSS).Method The clinical and pathological data of 8 patients with pSS and IgA in Changzheng Hospital from 2004 to 2009 were analyzed.Results The average age of seven female and one male was (44±8) years old.Five patients presented with edema and proteinuria.Eight patients presented with microhematuria.The average 24-hour proteinuria was (3±4) g.Two patients had hypertension.Serum creatinine levels of two patients were higher than normal level,the others'were normal.Light microscopy examination showed three patients were mild mesangial proliferation with Lee's classification grade Ⅰ;five patients had global sclerosis with Lee's classification grade Ⅱ,Ⅲ and Ⅳ.Positive IgA was mainly found under immunofluorescence microscopy.Electronic microscopy showed no electron-dense deposits.Conclusion IgA nephrology secondary to pSS is different from primary IgA nephrology under immunofluorescence microscopy.

Objective To investigate the association of the two single nucleotide polymorphisms(SNPs) rs1386494 and G1463A of tryptophan hydroxylase 2 gene with depression in Yunnan Han population. Methods A case-control study Was designed by collecting 102 patients and 102 controls.Polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP)technique Was used to detect the rs1386494 and G1463A polymorphisms.All patients were evaluated with Hamilton Rating Scale for Depression(HAMD),and scores were compared according to different genotype.Results 1.There were differences in genotype and allele frequency of rsl386494 in Yunnan Han population(X~2=4.300,P=0.038;X~2=4.067,P=0.044).The A allele frequency of rsl386494 in controls Was higher than in patients(P=0.044).2.No genotype of G1463A polymorphism was observed in all samples.3.No significant association genotype of rsl386494 with scores of HAMD Was observed(F=2.461,P=0.120).Conclusion The polymorphism of rsl386494 may be associated with the vulnerability of Yunnan Han population,and the A allele maybe the protective gene for depression.

Objective To observe the influence of low protein diet with α-keto acid on kidney sclerosis and renin-angiotensin system in renal ablation rats. Methods Chronic renal failure rat model was established by renal ablation in 30 male SD rats,then the animals were randomly assigned to the following diet groups:normal protein group (NPD:18%casein protein),low protein group (LPD:6%casein protein) and supplemented low protein group (LK:5%casein protein+1%α-keto acids).Ten male SD sham-operated rats received 18%casein protein as control.All the rats were killed at the end of the 12th week.Pathologic changes were assessed by PAS staining.Ang II in homogenate and plasma were measured by radioimmunoassay and ELISA respectively.Immunohistochemistry and Western blotting were used to detect the protein expression of TGF-β1,renin and AT1R.Real-time PCR was used to detect the gene expression of renin and ATla,the main subtype of AT1 receptor. Results Body weight,total protein and serum albumin had not significant difference among the four groups(all P>0.05).Serum creatinine and proteinuria of nephrectomized rats were significantly higher compared to the control group (all P<0.05).Proteinuria of the LK group was lower than that of NPD and LPD groups (all P<0.05).Pathological results indicated fibrosis indices were significantly improved after LPD and LK intervention.Expressions of renin,Ang II and AT1R in LK group were significantly lower than those in NPD group (all P<0.05). Conclusions Low protein diet with α-keto acids supplement therapy exhibits renal protective effects of reducing urine protein excretion and improving renal fibrosis,which might be related to the attenuation of local renin-angiotensin system in activity nephrectomized rats.

BACKGROUND:It is suggested that glucose metabolism of frontal lobes,cingulate gyri and caudate nuclei in cerebral functional area of patients with depression is low,and the blood flow in cerebellum decreases.OBJECTIVE:To observe the effect of scalp acupuncture on cerebral glucose metabolism in depression by positron emission tomography.DESIGN:Randomly controlled and verifying experiment.SETTING:Department of Acupuncture and Aoxibustion in Nanfang Hospital and Center of Positron Emission Tomography in a Guangdong People's hospital.PARTICIPANTS:Twelve patients of primary depression,from Nanfang Hospital,Zhujiang Hospital and Center of Positron Emission Tomography in Guangdong People's Hospital from October 2002 to October 2003,were selected randomly with the diagnostic criteria of [F32] in the Third Edition of the Chinese Classification and Diagnostic Criteria of Mental Disease.Among them,3 were male and 9 were female.Their ages were from 36 to 66 years,with the average of 48.METHODS:Giving scalp acupuncture in middle line of vertex(MS5),middle line of forehead(MS1) and bilateral lateral line 1 of forehead(MS2).All the patients received positron emission tomography examination before and after acupuncture treatment for 6 weeks.Then cerebral Regions of Interest,bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella,were chosen and their average glucose metabolisms were counted by half-quantifying analysis.MAIN OUTCOME MEASURES:Glucose metabolism of bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella.RESULTS:According to intension,all 12 patients with primary depression sults of right frontal lobes(2.91 ±0.34),left frontal lobes(2.96±0.26),right cingulate gyri(2.68±0.20),right caudate nuclei (2.61±0.39) and left cerebellum(2.55±0.18)were obviously higher than those of right frontal lobes(2.53±0.31),left frontal lobes(2.53±0.36),right cingulate gyri(2.34±0.31),right caudate nuclei(2.40±0.33) and left cerebellum(2.35±0.25)before treatment(P＜ 0.05-0.01).However, the results of left cingulate gyri (2.69 ±0.38),left caudate nuclei(2.66±0.01) and right cerebellum(2.50±0.27) were similar to those of left cingulate gyri(2.43±0.38),left caudate nuclei(2.45±0.21) and right cerebellum(2.30±0.34) before treatment(P ＞ 0.05).CONCLUSION:Scalp acupuncture on middle line of vertex,middle line of forehead and bilateral lateral line 1 of forehead could improve the glucose metabolism in depressive patients of certain cerebral regions,and it might be a mechanism related to improvement of frontal lobes,caudate nuclei,cingulate gyri and cerebellum.

SCI-E and SSCI covered papers on psychiatry published from 2002 to 2011 by authors of China, USA and Japan were analyzed using bibliometrics and the number, relative impact power and international cooperation of these papers were compared among the 3 countries, which showed that both the number and impact power of SCI-E and SSCI covered papers on psychiatry published by authors of China increased, the number of papers published by authors of China was catching up with that published by authors of Japan, and the impact power of papers published by authors of China was higher than that published by authors of Japan.However, they were significantly lower than those published by authors of USA.It is quite important to publish papers in journals with a high academic level and to strengthen international cooperation for increasing the domestic scientific research level in psychiatry.