1.Pediatric pilocytic astrocytoma with monomorphous pilomyxoid features and plexiform pattern: report of a case.
Chinese Journal of Pathology 2011;40(8):565-566
Astrocytoma
;
metabolism
;
pathology
;
surgery
;
Brain Neoplasms
;
metabolism
;
pathology
;
surgery
;
Child, Preschool
;
Diagnosis, Differential
;
Ependymoma
;
metabolism
;
pathology
;
Female
;
Follow-Up Studies
;
Glial Fibrillary Acidic Protein
;
metabolism
;
Humans
;
Neoplasm Recurrence, Local
;
Neoplasm Staging
;
Neurofibroma, Plexiform
;
metabolism
;
pathology
;
S100 Proteins
;
metabolism
;
Vimentin
;
metabolism
2.Clinical study of reformed laparoscopic-assisted vaginal hysterectomy of large uterus with sutureless of cardinal and uterosacral ligament
Xinling ZHOU ; Xinyong JING ; Yi LI
Chinese Journal of Postgraduates of Medicine 2011;34(36):11-13
Objective To compare the clinical efficacy between reformed laparoscopic-assisted vaginal hysterectomy with sutureless of cardinal and uterosacral ligament(LAVH)and trans abdominal hysterectomy(TAH)for large uterus.Methods A retrospective study was performed using 106 reformed LAVH patients(observation group)and 100 TAH patients(control group)whose uterus were big(≥ 12weeks of gestation),the information was observed in perioperative period.Results Anal exsufflation time,hospitalization time after operation and postoperative pain time in observation group[(25.4 ± 3.3)h,(6.5 ±1.5)d,(16.9 ± 2.4)h]were significantly shorter than those in control group[(42.4 ± 5.3)h,(8.5 ± 1.5)d,(30.6 ± 5.3)h](P < 0.05),the rate of complication in observation group[5.7%(6/106)]waa lower than that in control group[10.0%(10/100)](P< 0.05).Conclusion As to large uterus,reformed LAVH has several advantages over TAH,the technique is easy to learn,operation time is short,and the rate of complication is low.
3.The Comparative Study of Intonation Pronunciation in Preschool Children with Hearing Impairments and Normal Children
Ling YI ; Lei ZHANG ; Jing ZHOU
Journal of Audiology and Speech Pathology 2016;24(3):224-227
Objective This study aims to study the characteristics of intonation pronunciation in preschool children with hearing impairments .Methods A total of 30 of 4~5 years old children with hearing impairment and 30 of 4~5 years old children with normal hearing were enrolled in this study .With statement intonation and ques-tion intonation as test material ,the compartment between the children in two groups were obtained .Results Pre-school children were generally increased the ending point and slope to distinguish statement intonation .There were still differences between hearing -impaired children and normal children .Under statement intonation condition :the children with hearing impairments showed a significantly lower level of the slope of the high and level boundary tone than the normal children ,the starting point and the ending point of the falling boundary tone in hearing -impaired children were significantly lower than those of in normal children ;Meanwhile ,under question intonation condition :com-pared with normal children ,the slope of the high and level boundary tone ,the rising boundary tone and the falling -rising boundary tone in hearing -impaired children were significantly lower .The starting point ,the ending point ,the high and level boundary tone ,and the falling boundary tone in hearing -impaired children were significantly lower .Conclusion To implement targeted therapy during question intonation training for hearing -impaired children ,the acoustics characteristics of boundary tone are required to assess and monitor the effectiveness of rehabilitation .
4.Specific allergic food Intolerance and correlated factors in Qingdao areas
Yan WANG ; Ying ZHANG ; Jing ZHOU ; Wei WANG ; Yi ZHANG
Chinese Journal of Health Management 2012;06(3):154-157
ObjectiveTo study specific allergic IgG antibody test and its impact factors.MethodsA total of 1770 Qingdao residents were recruited in this cross-sectional study from January to October 2011.ELISA was used to test specific allergic IgG antibodies.Logistic regression was used for multifactor analysis.Results Of 1770 participants,9 individuals had insufficient data,resulting in a response rate of 99.5%.Identification rate of specific allergic IgG antibody was 60.0% (1056/1761 ),and the most commonly seen allergic foods were crab (49.4% ),egg (44.80% ),ling (38.0% ),shrimp ( 30.4% ) and soy ( 22.2% ).Intolerance to crab,egg,shrimp,creamery,com,and tomato showed gender difference ( x2 =18.978,P<0.05 ).Serum level of allergic IgG antibody was increased with age [20 - 40 years:( 118.61 ±45.67) U/ml; 41-60 years:(166.57 ±55.82) U/ml; >60 years:(183.67 ±49.34) U/ml; x2=13.597,P<0.01].In logistic regression,difference between individuals with normal body weight and obesity population ( OR =1.897,95% CI 1.215 - 2.578 ) or between those with or without allergic history ( OR =0.780,95% CI 0.648 - 0.912) was found.Conclusions Impact factor of food intolerance includes gender,age,constitution and body mass index.Six kinds of food intolerance are more common in women.41 -60 year group shows peak of food intolerance.No exposure to allergic food and antianaphylaxis may reduce the risk of food intolerance.
5.Effects of dexmedetomidine on PI3K/Akt pathway in hippocampus of propofol anesthetized neonatal rats
Lifang ZHOU ; Yi WEI ; Jing LYU ; Yubo XIE
The Journal of Clinical Anesthesiology 2017;33(1):59-62
Objective To explore the effect of dexmedetomidine on phosphoinositide 3-kinase/protein kinase B (PI3K/Akt ) pathway in hippocampus of propofol anesthetized neonatal rats. Methods Eighty Sprague-Dawley male rats,aged 7 days,weighing 10-1 5 g,were randomly divided into 8 groups (n= 10 each):normal saline group (group N),DMSO group (group D),intralipid group (group I),propofol group (group P),dexmedetomidine 25 μg/kg,50 μg/kg and 75 μg/kg +propofol 100 mg/kg groups (groups PD25 ,PD50 and PD7 5 ),LY294002 25 μg + dexmedetomidine 75μg/kg + propofol 100 mg/kg group (group LYPD).The hippocampus of rats in all groups were taken 2 h after the animals fully awake.The ultrastructure of hippocampal neurons was observed by transmission electron microscope.The pAkt-(ser473 )protein and Akt protein in the hippocampus were evaluated by Western blot analysis.Results There was no significant difference in the expression of Akt protein among the eight groups.Compared with group N,the expression of pAkt (ser473)protein was significantly down-regulated in groups P,PD25 ,PD50 ,PD7 5 and LYPD (P <0.05).Compared with group P,the expression of pAkt (ser473)protein was increased significantly in groups PD7 5 and LYPD (P <0.05).Compared with group PD7 5 ,the expression of pAkt (ser473) protein was significantly down-regulated in group LYPD (P <0.05 ).The structure of hippocampal neurons was normal in groups N,I and D.Nuclear nuclei swelling,chromatin decreasing and mito-chondrion vacuolar degeneration were observed in group P while improved gradually with dexmedeto-midine in a dose-dependent manner in groups PD25 ,PD50 and PD7 5 .Neurons karyopyknosis,partial dissolution of nuclear membrane,chromatin condensation,mitochondria vacuolar degeneration were observed in group LYPD.Conclusion Dexmedetomidine pretreatment provides neuroprotection against propofol-induced hippocampal destruction by preserving PI3K/Akt pathway activity in the de-veloping brains.
6.The role of NF-?B signal pathway in a rat acute colitis model
Jing ZHOU ; Yi FENG ; Shan WANG ; Yingjiang YE
Chinese Journal of General Surgery 1993;0(03):-
Objective To investigate the role of NF-?B signal pathway in the stress of a rat acute colitis model. Methods Acute colitis were induced by 6% intracolonic acetic acid lavation in rats; the spinal I-?B levels were measured by Western blot at L5-S1 and C2-4 levels; the spinal COX-2 mRNA levels were measured by RT-PCR at the same segments; the expressions of spinal COX-2 were also measured by immunohistochemistry. Results There were obvious regional inflammatory responses after intracolonic acetic acid lavation in rats; the extent of inflammation was gradually aggravated with time.The expressions of spinal I-?B at L5-S1 level significantly increased after 0. 5 h in acute colitis group. Expressions of experimental group were significantly higher than that of sham group at half an hour(P
7.A study on early hearing impairment with essential hypertension
Jing ZHANG ; Huifang ZHOU ; Geng ZHANG ; Yi XU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;(16):731-733
Objective:To study the characteristic of hearing loss in essential hypertension. Method:Sixty-eight cases (136 ears) of patients with essential hypertension were divided into two groups, i. e. group A, patients without retinal alteriosclerosis (35cases, 70 ears ) and group B, patients with retinal alterioselerosis (33cases, 66 ears ). The control group consisted of 30 cases (60 ears) of the same sex and same age. AH people were measured by pure tone audiometry and distortion product otoacoustic emissions. Result:The pure tone thresholds measured in group B hypertensive patients were significantly higher than in the normal controls (P<0. 05) , whereas pure tone thresholds in group A were not different from those in the control group(P>0. 05). From 1000 to 8 000 Hz , DPOAE amplitudes of hypertensive group A and group B were lower than that in control group(P<0. 01). Only on the frequency points of 4 000 Hz , the detection rate of DPOAE in group B was lower than that in control group (P<0. 05). Conclusion: Hypertension is a risk factor of degeneration of the hearing apparatus. The hearing function of hypertensive patients has probability to be impaired, although they do not feel hearing loss.
8.Mechanism about therapeutic effect of meisoindigo on psoriasis via down-regulation of the TLR4-TAK-NF-kappaB pathways.
Haijing ZHANG ; Yi ZHANG ; Jing JIN ; Wanqi ZHOU ; Xiaoguang CHEN
Acta Pharmaceutica Sinica 2013;48(4):503-7
Meisoindigo is an indigo natural derivative commonly used in anti-cancer therapy. In the clinical application, it was also found to have good therapeutic effect on psoriasis. In order to further understand its mechanism of action, human normal keratinocyte cell line HaCaT and RAW 264.7 were used to identify if meisoindigo could affect the inflammatory factors such as NO and other important cytokines which were highly involved in psoriasis. Our results indicated that meisoindigo decreased the production of NO in LPS-stimulated RAW 264.7 cells and reduced the expression of cytokines in LPS-stimulated HaCaT cells. And TLR4-TAK-NF-kappaB was a possible pathway mainly involved in the attenuation of iNOS and pro-inflammatory cytokine production by meisoindigo, which may take part in the therapeutic effect of meisoindigo on psoriasis.
9.Practical consideration on vascular patterns in pathologic diagnosis of central nervous system tumors.
Jing ZHOU ; Guo-yi YANG ; Nan-yun LI
Chinese Journal of Pathology 2011;40(9):641-644
Astrocytoma
;
blood supply
;
pathology
;
Capillaries
;
pathology
;
Central Nervous System Neoplasms
;
blood supply
;
pathology
;
Diagnosis, Differential
;
Ependymoma
;
blood supply
;
pathology
;
Glioblastoma
;
blood supply
;
pathology
;
Hemangioblastoma
;
blood supply
;
pathology
;
Humans
;
Hyperplasia
;
Microvessels
;
pathology
;
Neoplasms, Neuroepithelial
;
blood supply
;
pathology
;
Oligodendroglioma
;
blood supply
;
pathology
;
Paraganglioma
;
blood supply
;
pathology
10.Effect of 3-n-butylphthalide pretreatment on expression of the HSP70 after brain ischemia/reperfusion.
Yi ZHOU ; Li-jing NIU ; Feng-miao QI ; Li GUO
Chinese Journal of Applied Physiology 2015;31(2):136-140
OBJECTIVETo explore the effect of 3-n-butylphthalide pretreatment on the delayed neuronal death(DND) and the expreesion of heat shock protein70 (HSP70) in rat hippocampus after ischemia/ reperfusion.
METHODSAll rats were randomly divided into sham group (n = 36), total cerebral ischemia (TCI) group (n = 36), butylphthalide (NBP) group (n = 6), NBP + TCI group( n = 36), quercetin + NBP + TCI group (n = 6), dimethyl sulfoxide (DMSO) + NBP + TCI group (n = 6). The model of total cerebral ischemia/reperfusion was established by blocking vertebral arteries and carotid arteries. In sham group, TCI group and NBP group, the animals were further divided into instantly, 6 h, 12 h, 1 d, 3 d, 5 d groups according to the time interval after sham operation or TCI. Histological changes of the hippocampus were evaluated using thionin staining under light microscope by determining the delayed neuronal death (DND) and the expression of HSP70 was assayed using immunohistochemistry.
RESULTSNBP pretreatment could reduce delayed neuronal death in CA1 of hippocampus induced by TCI-reperfusion injury in rats, and up-regulated the expression of HSP70 in CA1 hippocampus of brain ischemic/reperfusion for 5 days. Quercetin blocked the acquirement of the brain ischemic tolerance induced by NBP preconditioning.
CONCLUSION3-n-butylphthalide (NBP) prevents the neurons from ischemia/reperfusion injury through upregulating the expression of HSP70.
Animals ; Benzofurans ; pharmacology ; CA1 Region, Hippocampal ; cytology ; pathology ; Cell Death ; Cerebral Infarction ; drug therapy ; HSP70 Heat-Shock Proteins ; metabolism ; Ischemic Preconditioning ; Neurons ; cytology ; Rats ; Rats, Wistar ; Reperfusion Injury ; drug therapy