1.Early predictive value of platelet related indicators in patent ductus arteriosus in extremely low birth weight infants
Junjuan ZHONG ; Zhongwei YAO ; Jing MO ; Jing ZHANG ; Jie YANG
Journal of Clinical Pediatrics 2017;35(4):273-277
Objective To analyze the predictive value of platelet related indicators for patent ductus arteriosus (PDA) in extremely low birth weight infants (ELBW). Methods The data of 79 ELBW infants born from June 2013 to June 2016 were retrospective analyzed. There were 48 cases without PDA (nPDA group) and 31 cases with PDA (PDA group). Among 31 cases with PDA, there were 17 cases of non-haemodynamically significant PDA (nhsPDA group) and 14 cases of haemodynamically significant PDA (hsPDA group). The clinical feature and platelet related indicators among nPDA group, PDA group, nhsPDA group and hsPDA group were compared. Multivariate logistic regression was used to analyze the effects of various factors on the occurrence of PDA. ROC curve analysis was performed to evaluate the early predictive value of platelet related indicators for PDA. Results Compared with the nPDA group, the PDA group had a smaller gestational age, a higher proportion of male infants, and a smaller platelet distribution width (PDW), and there were statistically significant differences in all of those (P all<0.05). Multivariate logistic regression analysis indicated that the risk of PDA was increased as the PDW was decreased (OR=1.26, 95%CI: 1.05~1.52). The ROC curve analysis showed that the best diagnostic value of PDW was 13.4 GSD, and the sensitivity of early prediction of PDA was about 67.74%, and the specificity was 68.75%. Compared with nhsPDA group, hsPDA group had a smaller gestation age, lower cesarean section rate, and there were statistically significant differences (P all<0.05). There was no significant difference in platelet related indicators between hsPDA group and nhsPDA group (P>0.05). Conclusion PDW has certain early predictive value for PDA in ELBW. ELBW infants with PDW<13.4 GSD need to be watched closely for the occurrence of PDA.
2.Analysis of the Difference in Knowledge Attitude and Behaviour of Hypertension Patients pre and after Intervened
Jufeng YAO ; Yajing ZHANG ; Jing SUN ; Jimin ZHANG ; Wen FENG
Chinese Journal of Practical Nursing 2001;17(2):1-3
To search the effective health education to the hypertension patients and to decrease complication such as cerebrovascular ailment,106 hypertension patients were divided into two groups randomly:the interference (52) and the control (54).The interference group is treated with health education and health promotion constantly (Base line:1996,end line 1999).The results of 2 times investigations at the base line and end line respectively to the groups show that:the KAB in both groups were improved,and the interference improved notably,especially in behavior.The percentage of the patients who care about danger factors and hygeine knowledge rise from 68% (base line) to 78.6% and the percentage of the patients who did exercise constantly rise from 58.9% to 71.4%.It is also showed that,in periodic measurement of blood pressure and regular administration of medicine,the interference group behaved better than the contrast (P<0.01).We can conclude that giving face to face health education and health promotion to hypertension patients is a effective way to prevent and control hypertension complications
3.Viral dynamics during entecavtr therapy in patients with chronic hepatitis B
Bilian YAO ; Xinxin ZHANG ; Jing YANG ; Demin YU ; Donghua ZHANG
Chinese Journal of Infectious Diseases 2009;27(6):338-342
Objective To analyze the viral dynamics and clinical significance during the antiviral treatment by a mathematical model.Methods Six chronic hepatitis B patients were evaluated with a kinetic model(Neumann model)during dose of 0.5 mg/d oral entecavir.Blood samples were drawn for HBV DNA measurement at week 0,2,4,12,24.Non-linear modeling was used to fit individual patient data.Results The median effectiveness in blocking viral production was 99.970%(n=6).The median half-life of viral turn-over was 1.6 d(n=6).The median half-life of infected hepatocytes was 21.3 d(n=5).Compared with the other patients,the c(virions are cleared at a rate)、ε(effectiveness)、δ(infected cell are lost at a rate)value of one patient(eg.6)were all lower and the half-life of virus and infected cells were higher,and eg.6 developed viral break-through after 38 weeks of follow-up.Conclusions Viral load decay showed a biphasic pattern during entecavir therapy which can be described with a mathematical model.The model relates processes of viral infection and replication as well as drug efficacy to model parameters.It indicates the prediction of bio-mathematical model during antiviral treatment.
4.Analysis of the Difference in Knowledge Attitude and Behaviour of Hypertension Patients pre and after Intervened
Jufeng YAO ; Yajing ZHANG ; Jing SUN ; Jimin ZHANG ; Wen FENG
Chinese Journal of Practical Nursing 2001;0(02):-
To search the effective health education to the hypertension patients and to decrease complication such as cerebrovascular ailment,106 hypertension patients were divided into two groups randomly:the interference (52) and the control (54).The interference group is treated with health education and health promotion constantly (Base line:1996,end line 1999).The results of 2 times investigations at the base line and end line respectively to the groups show that:the KAB in both groups were improved,and the interference improved notably,especially in behavior.The percentage of the patients who care about danger factors and hygeine knowledge rise from 68% (base line) to 78.6% and the percentage of the patients who did exercise constantly rise from 58.9% to 71.4%.It is also showed that,in periodic measurement of blood pressure and regular administration of medicine,the interference group behaved better than the contrast (P
5.Measurement and analysis of 210Pb radioactivity level in outdoor air during spring in Beijing
Shuaimo YAO ; Fei TUO ; Qing ZHANG ; Qiang ZHOU ; Jing ZHANG
Chinese Journal of Radiological Medicine and Protection 2017;37(4):286-289
Objective To measure and analyze the radioactivity level of 210Pb in outdoor air in Beijing in spring.Methods Portable high flow air samplers were used to collect outdoor air at the ground level to analyze the 210Pb radioactivity in the aerosol filter samples using a laboratory-based high purity germanium (HPGe) γ spectrometer.Results The activity concentration of 210Pb outdoors ranged from 267.2 to 1 697.6 μBq/m3,with an average of (878.7 ± 386.7) μBq/m3.Statistical analysis showed that the activity concentrations 210Pb of outdoors varied with variable air quality.Conclusions The activity concentrations of 210Pb outdoors are detectable in Beijing,varying considerably but within the normal range.
6.Metabonomic Study on Fatigue Elimination of Exhaustive Exercise Mouse by Rhodiola Based on UFLC-Q-TOF
Yao HU ; Jing ZHANG ; Yan ZHANG ; Ping WANG ; Xianli MENG
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(11):2209-2214
The liquid-chromatography-mass technology was used in the metabolomic analysis of mouse's blood 1 hour after exhaustive exercise,in order to explore the potential mechanism of Rhodiola in fatigue elimination of exhaustive exercise mouse.The exhaustive mouse model was made by loaded-swimming.A total of 24 mice were randomly divided into theRhodiola + exercise group,exercise group andno-exercise group.The dose of Rhodiola was 0.4375 g·kg-1·d-1.The loaded-swimming was conducted after two successive weeks of medication.Blood was collected 1 h after swimming for the sample preparation.The enzyme assay and anthrone colorimetry were used to test blood lactate acid and glucose,respectively.UFLC-Q-TOF was used to detect metabolic profiles of each group.The principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) and heat map analysis were used to compare differences among groups with score chart and to obtain the characteristics biomarkers by load chart.The results showed that the blood lactate acid level of theRhodiola+ exercise group was significantly lower than that of theexercise group.And the glucose level of theRhodiola+ exercise group was significantly higher than that of theexercise group.The metabolomic analysis showed that there were no obvious changes on 1,25-(OH)2D3,diacylglycerol (DG) and inositol triphosphate (IP3).All three materials in theRhodiola + exercise group were significantly lower than those of theexercise group.They were much closer to theno-exercise group.And all three materials were related to the increasing of muscle tension.It was concluded that Rhodiola had the function of promoting fatigue eliminating.This effect may be related to cell membrane protection,regulation of 1,25-(OH)2-D3→IP3,DG pathway,and relieving of muscle tension after exercises.
7.Effects of pulsed electromagnetic fields on the expression of MyoD after acute skeletal muscle contusion
Jing WANG ; Changjie ZHANG ; Xudong GU ; Yunhai YAO
Chinese Journal of Physical Medicine and Rehabilitation 2009;31(5):292-295
Objective To observe the effects of pulsed electromagnetic fields (PEMFs) on histological changes and myogenic differentiation factor D (MyoD) expression in rats with acute skeletal muscle contusion ( ASMC), and to explore the effects of PEMF therapy on rats with ASMC in its very early stages. Methods Forty-two rats were randomly divided into three groups : a treatment group, a control group and a blank control group. ASMC models were established with all the animals in the treatment and control groups. PEMF treatment was admin-istered to the treatment group immediately after the establishment of the ASMC model. Seven rats in each group were sacrificed at the 12th and 18th h after the models were set up. Their triceps surae muscles were sampled and treated with haematoxylin-eosin staining for study using immunofluorescence techniques and a fluorescence microscope. Re-suits In the control group at the 12th h and 18th h, HE staining showed pale cytoplasm and polymorphism in the cell nuclei ; in the treatment group these effects were significantly lighter, but in both groups it was more serious than in the blank control group. In the treatment and control groups, the fluorescence intensity of MyoD at the 18th h was higher than at the 12th h, and at each time point in both groups it was higher than in the blank control group. At the 18th h, fluorescence in the treatment group was stronger than in the control group. Conclusion MyoD expression in rats with ASMC is upregulated by thel8th h after early PEMF treatment. This might be one of the mechanisms ac-celerating the regeneration of skeletal muscles after trauma.
8.Preliminary exploration of the frontal-cingulate functional connection during identifying sad facial expression in depressed patients
Gang LIU ; Qing LU ; Jing ZHANG ; Haiyan LIU ; Zhijian YAO
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(12):1057-1059
Objective To analyze the difference of frontal-cingulate functional connection between patients with major depression and healthy controls in recognization of sad facial expression, and discuss the neutral basis of emotional bias in preliminary. Methods 12 female patients with major depression and 12 matched healthy controls were scanned. The intensities of brain activation in the process of sad and neutral facial expression recognition were analyzed, and the strengths of frontal-cingulate functional connection were compared between the two groups based on dynamic causal modeling. Results Comparing the stimulus adjusting connection models with Bayesian model indicated that model Ⅱ matched with the observation data better. In the comparison between model Ⅱ and model I ,the average Bayes factor was more than 7.38. In the comparison between model Ⅱ and model Ⅲ,the average Bayes factor was more than 2.71. Calculating the intrinsic connection parameters and stimulus adjusting connection parameters based on intrinsic connection model and stimulus adjusting connection model( model Ⅱ ), the result showed that connection strength between anterior cingulate and right inferior frontal gyrus in depression was greater than that in healthy controls. Conclusion The frontal-cingulate functional connection of patients with major depression is increased abnormally ,which may be the neural basis of negative emotional bias.
10.Change of airway anaphylatoxin C5 a in patients with asthma
Jing ZHANG ; Chun CHANG ; Ming LU ; Yahong CHEN ; Wanzhen YAO
Journal of Peking University(Health Sciences) 2015;(1):145-148
Objective:To investigate the role of anaphylatoxin C 5 a in patients with asthma .Methods:A prospective study was performed between September 2006 and February 2007.A total of 33 patients with acute exacerbation of asthma and 13 healthy subjects were recruited into the study .The patients with acute exacerbation of asthma were also studied when they returned to the remission state .Levels of lung function, levels of C5a in induced sputum and cell differential count in induced sputum were determined . Results:The level of C5 a in induced sputum was significantly higher in patients with acute exacerbation of asthma [0.85(0.68-2.13) μg/L] than that in patients with stable asthma [0.45(0.26-0.88)μg/L, Z=-2.193, P=0.013];Sputum C5a levels in stable asthma patients were significantly higher than those in healthy controls [0.14(0.06-0.45) μg/L, Z=-2.141, P=0.015].The level of C5a in patients with severe exacerbation [2.21(1.27 -9.0) μg/L] was significantly higher than those in patients with mild exacerbation [0.34(0.17-0.63) μg/L] and moderate exacerbation [0.85(0.55-1.67) μg/L,χ2 =12.330, P=0.001].The level of C5a in induced sputum was positively correlated with the number of total cells count (r=0.797, P=0.004), neutrophils (r=0.504, P=0.032) and macrophages ( r =0.424, P=0.036 ) in acute exacerbation of asthma .Conclusion: C5a levels in induced sputum could be identified as an important prognostic biomarker , which involved in asthma ’ s pathogenesis .