Objective To evaluate the effect of intra-arterial infusion of basic fibroblast growth factor(bFGF) on improving neovascularization, vascular perfusion and the function of partially ischemic limbs of rabbits. Methods Twenty-seven New Zealand male rabbits were selected. Partial ischemia model was induced by surgical ligation of the primary branches of right femoral artery in each animal, and the left hind limb of each animal was served as a nonischemic control. Then, 27 rabbits were randomly assigned to three groups: intra-arterial (IA) infusion of bFGF (n=9), intravenous (IV) infusion of bFGF and IA infusion of saline (n=9). Infusion was separately performed immediately after vascular ligation, 8th and 15th days post-surgery with 10 ?g (4 ml) of bFGF per-time (or the same volume of saline). The differences between three groups and between ischemic and nonischemic limbs of the same group were compared and evaluated by the following indexes: (1) vessel section count (VSC), vessel section surface area (VSS) and vessel section perimeter (VSP) in the field of ischemic muscle tissues taken at 22nd day postoperatively; (2) capillary refilling time of ischemic limbs; and (3) functional and trophic changes of ischemic limbs. Statistical differences were evaluated by one-way ANOVA and T test. Results VSC, VSS and VSP of the IA-bFGF group were significantly increased than those of the IV-bFGF and IA-saline groups (P

Objective To explore the changes of the levels of IL-8,LTB4 and its function in invasion in patients with chronic obstructive pulmonary disease(COPD).Methods The concentrations of IL-8 and LTB4 in serum and sputum were determined with enzyme linked immunosorbent assay(ELISA)in 30 cases of patients with COPD in acute aggravating stage and paracmasis and in 20 cases healthy adults.Blood routines were detected in all obstructs.Results The concentrations of IL-8 in serum and sputum in acute aggravating stage were(181.41±18.49)ng/L and(74.03±20.64)ng/L,significantly higher than those in paracmasis and the reference group(152.33±17.48)ng/L and(42.30±14.99)ng/L,P＜0.05).The concentrations of IL-8 in serum and sputum in paracmasis were significantly higher than those in reference group[(52.75±13.79)pg/ml and(17.15±5.36)ng/L,P＜0.05].The concentrations of LTB4 in sputum in acute aggravating stage were(2300.91±496.01)ng/L,significantly higher than those in paracmasis and the reference group[(1884.14±651.37)ng/L,P＜0.05].LTB4 level in acute aggravating is not significantly different from those in paracmasis and the reference group[(1941.30±301.62)ng/L and(1880.77±302.11)ng/L,P＞0.05).Conclusion Both IL-8 and LTB4 play important roles in the coupe of inflammatory reaction of air passage of COPD.

Objective To study the indication, efficacy, safety, anesthesia method, and complication of extracorpereal shockwave lithotripsy (ESWL) in combination with endoscopic retrograde eholangiopanereatography (ERCP) for pancreatic duct stones. Methods From November 2011 to February 2016, we observed the success rate and safety of ESWL in combination with ERCP in treatment of 33 patients with chronic pancreatitis and large pancreatic duct stones and evaluate the efficacy in 6 month and 4 years follow-up. Results All the 33 patients underwent 52 ESWL, the successful fragmentation rate was 100.00%; complete clearance was achieved in 27 patients (81.82%) under the treatment of ESWL in combination with ERCP. Their pain and steatorrhea relieved, the weight increased. Conclusions ESWL combined with ERCP is effective, safe for chronic pancreatitis with pancreatic duct stones.

Changes of ventricular relaxation, contractility and compliance and their effects on cardiac pump function during the development of chronic high output heart failure were studied in the animal model produced by establishment of combined aorto-caval fistula (ACF) and unilateral renal artery stenosis (RAS) in rats.In the present study, all the rats with ACF+RAS showed the characteristics of high output hear failure of varying degree, the cardiac index rised significantly even at rest, while pump function reserving force (CORF) decreased to different extent.The resu(?)lts show that with the aggravation of cardiac pump function deterioration, ventricular relaxation and contractility decreased progressively, the ventricular diastolic compliance rised significantly. With stepwise regression analysis, the changes of ventricular relaxation, comtractility and compliance have different effects on cardiac pump function at different stage of heart failure.

Humans ; Podocytes ; physiology ; Proteinuria ; physiopathology

Objective:To study the inhibition of astragaloside on the proliferation of human keloid fibroblasts. Methods: Com-pared with that of normal skin, the expression of transforming growth factor-β( TGF-β) and its transduction factors Smad in the human keloid fibroblasts was detected. The optimal concentration was screened by MTT after HFF-1 human skin fibroblast was infected with astragaloside at different concentrations. The mRNA expression of Smad2, Smad3, Smad4 and Smad7 in the fibroblasts was studied by using real-time. The protein expression of TGF-βRⅡ, Smad2, Smad3, Smad4 and Smad7 in the fibroblasts was detected by using Western blot. Results: Compared with that of normal skin tissue, the expression of Smad protein was significantly increased ( P <0. 05) in the human keloid fibroblasts, and there was no significant difference in the TGF-βRⅡ expression (P>0. 05). The optimal concentration of astragaloside was 0. 5μg·ml-1 . The expression level of Smad2 protein in the two groups was significantly increased, and the level of Smad3 expression was significantly decreased (P<0. 05). Conclusion:Astragaloside can inhibit the formation of fi-broblast possibly through Smad2 over-expression and Smad3 inhibition in the TGF-β/Smad signal transduction pathway.

Objective To discuss the management of bile duct injuries during laparoscopic cholecystectomy(LC).Methods From March 1992 to October 2006,totally 8876 cases of LC were performed in our hospital.Bile duct injury occurred in 16 of them,among which,the common bile duct was mistakenly cut in 4 cases,partly torn in 5,and wrongly clipped in 5,and electrical burn injury happened in 3.The patients received bile duct repair and T-tube drainage(6),bile duct anastomosis plus T-tube drainage(3),or Roux-en-Y bowel biliary anastomosis(5) after the accidents.In 2 patients,the titanium clips were removed.Results In one of the patients with the bile duct been cut,bile duct anastomosis was performed,and T-tube drainage was continued for 3 months;however,3 to 5 months after the T tube being removed,the patient had to underwent Roux-en-Y bowel biliary anastomosis because of biliary stenosis.No obstructive jaundice occurred in this patient after the Roux-en-Y.Two patients received a second t Roux-en-Y bowel biliary anastomosis owing to anastomotic stenosis.The second operation was carried out 9 or 10 month after the first surgery,and the patients were then followed up for 2 to 4 years.None of them developed cholangitis or calculi during the follow-up.One patient,who suffered a clipped bile duct,underwent Roux-en-Y bowel biliary anastomosis after the LC,but developed recurrent infection of the bile duct after the second operation.Among the 16 patients,12 were cured,10 of them were followed up for 3 to 4 years,during which no one showed biliary symptoms.Conclusions Bile duct injury is one of the major complications after LC.Early prevention and intervention are very important to avoid repeated operations.

Aim To investigate the effects of isoliquiritigenin(ISL) on cognition dysfunction and brain energy metabolism following repeated cerebral ischemia-reperfusion in mice.Methods Mouse models of learning and memory deficits were made by repeated occlusion of bilateral common carotid arteries and reperfusion. Step-down test and Y-maze task were used to examine the learning and memory abilities of mice. The brain energy state was analyzed by reversed-phase high performance liquid chromatogra phy. Results ISL 10, 20 and 40 mg?kg -1 ig reduced the n umber of errors and extended the latency of memory in step-down test, decreased the number of cumulated stimulation and increased the number of right choices i n Y-maze task. ISL(10, 20, 40 mg?kg -1) increased the levels of ATP, ener gy charge (EC) and total adenine nucleotides(TAN) in a dose-dependent manner. Conclusion The results suggest that ISL improves cognition dysfun ction induced by repeated cerebral ischemia-reperfusion in mice, which may be r elated to the improvement of brain energy metabolism.

Objective To explore the effect of Botulinum toxin-A (BTX-A) injected into the contralateral facial muscle on central facial palsy post stroke. Methods 30 stroke patients with moderate to severe central facial palsy were recruited (course of 3~10 months). They were divided into control group (n=15) and treatment group (n=15), who accepted facial training and BTX-A injection in addition, respectively.The bilateral deference of distance from angulus oris to the midline of the teeth(D1) and from the paropia to the angulus oris (D2) were measured before and 4 weeks after injection. Results The D1 and D2 both at resting and movement all decreased after injection in the treatment group, and decreased more than those in the control group. Conclusion BTX-A injection can further correct central facial palsy post stroke.