The fetal stage is a critical period for brain development. Intrauterine growth restriction(IUGR)af-fects the growth of fetus and impairs the brain development severely,resulting in long - term neurological sequelae such as motor and behavioral disorders. Since their brain underdevelopment originated in the uterus,postnatal interventions usually fail to improve the brain function. Then antenatal interventions become urgent to protect the brain,taurine has appeared a high practical value in improving the brain development. The adverse effects of IUGR on fetal brain develop-ment and the possibilities of antenatal interventions were introduced in this paper in order to improve the prognosis of the nervous system.

Objective The aim of this study is to investigate the effects of the initial amount of total RNA input,adapter dilution and PCR amplification cycles on the results of small RNA library preparation and high-throughput sequencing.Methods Based on the sequencing reads number,the number of detected miRNAs and the accuracy of quantitative detection,we explored the effects of initial Total RNA,dilution ratio and PCR cycles on the quality of microRNA library preparation and high-throughput sequencing,respectively.Results For libraries preparation of the normal RNA input(1 μg),the adapter dilution combined with 22 PCR cycles could gain the best quality of sequencing.In the low-input libraries(10 ng),adapter dilution and increased PCR cycles would also improve the quality of sequencing,but as compared with the 1 μg library,there was lower correlation with microarray quantitative results in general.Conclusions The initial amount of RNAs input has the biggest effects on the quality of sequencing,and the quantity accuracy rate of low-input libraries is lower than the normal libraries generally.Increasing the PCR amplification cycles properly is indispensable to low-input libraries.

Objective To investigate the risk factors of early recovery in patients with ischemic stroke(IS).Methods 168 patients with IS received the conventional treatment and early rehabilitation were enrolled in the study.Single factor analysis was used to analyze the risk factors of the IS in the early treatment of rehabilitation in patients with and without success recovery rehabilitation success,and then application (unconditional Logistic regression analysis factors early independent risk factors for healing.Results Single factor analysis shows that age quartile (60years old),history of hypertension,coronary heart disease,diabetes,history,history of smoking history,anxiety and depression and successful rehabilitation negatively,life,education degree by law quartile (12 years) and mental work positively correlated with successful recovery.Many factors analysis shows that education degree by quartile (12)(OR=0.12,95％ 00.03 to 0.75),mental workers (OR=0.45,95％ CI 0.37 to 0.47) is the success of the recovery protection factor (P＜0.05),And age quartile (60) ( OR=2.12,95％ CI 1.21 to 3.21 ),anxiety ( OR =2.64,95％ CI 1.82 to 6.11 ) and depression (OR =1.45,95％ CI 1.13 to 1.96) is the success of the recovery independent risk factors (P ＜ 0.05 ).Conclusion Age quartile (60),anxiety and depression are IS successful independent risk factors for the recovery of the patients with early rehabilitation IS the risk factors should undertake the necessary intervention.

Objective The purpose of this study was to explore the infection characteristic of Toxoplasma gondii (TOX),Rubella virus (RV),Cytomegalovirus (CMV) and Herpes simplex virus Ⅱ type (HSV-Ⅱ) (TORCH) infection in neonate in Tianjin area.Methods TOX-IgM/IgG,RV-IgM/IgG,CMV-IgM/IgG and HSV-Ⅱ-IgM/IgG were detected in serum of 2 273 neonate during 2015～2016 with enzymelinked immunosorbent assay (ELISA).Results The positive rates of TOX IgM,RV-IgM,CMV-IgM and HSV-Ⅱ-IgM were 0.00％(0/2 273),0.00％(0/2 273),0.88％(20/2 273) and 0.00％(0/2 273),respectively and those of TOX-IgG,RV-IgG,CMV-IgG and HSV-Ⅱ-IgG were 3.65％ (83/2 273),86.45％ (1 965/2 273),95.82％(2 178/2 273) and 8.27％(188/2 273),respectively.There was 0.66％ percent (15/2 273) of examinees who were infected by none of TORCH pathogens.There existed significant statistical difference for positive rate between TOX-IgG,RV-IgG,CMV-IgG and HSV-Ⅱ-IgG (x2 =6.747,P =0.000) with consequence of the highest positive rate being CMV-IgG.The positive rates of TOX-IgG and CMV-IgM in neonate of 2016 were significantly less than those in 2015 (x2 =5.789～7.505,P=0.006～0.016) but that of HSV-Ⅱ-IgG of 2016 was statistically higher than that in 2015 (x2 =6.073,P =0.014).The positive rate of CMV-IgM in male neonate in 2015 was significantly higher than that in 2016 (x2 =5.054,P =0.025).As a whole the positive rates of TOX-IgG,RV-IgG,CMV-IgG and HSV-Ⅱ IgG had no differences between different years,so did those between gender groups (x2 =2.23～6.963,P=0.073～0.526).The positive rates of TOX-IgG,RV IgG,CMV-IgG and HSV-Ⅱ-IgG in female neonate in 2015 were statistically different from those in 2016 (x2 =8.247,P =0.041).The female neonate in 2015 had higher infection proportion of TOX-IgG compared with that in 2016 (x2 =6.992,P=0.008).TORCH infection detected in 2 273 cases of neonate had one pathogen infection and multi-pathogen infection with overall six patterns of TORCH infection and all infection patterns had no relationship with year and gender,respectively (P＞0.05).Conclusion RV and CMV were primary pathogens in TORCH infection for neonate in Tianjin area and there were recent infections by CMV.TORCH infection varied in different years and gender groups,which provided experimental data and basis for epidemiology and prevention of TORCH in neonate.

OBJECTIVETo observe the curative effect of Qianjin Kouchuang Jiawei Granule (QKJG) on recurrent oral ulceration [yin deficiency fire excess type (YDFET)].

METHODSTotally 120 patients who suffered from recurrent oral ulceration (YDFET) were randomly assigned to two groups, the experiment group and the control group, 60 in each group. Patients in the experiment group took QKJG, 20 g each time, twice per day, while those in the control group took Kouyanqing Granule (KG) , 20 g each time, twice per day. Fourteen days consisted of one therapeutic course, two for all. Scores for patients' symptoms and signs (ulcer area, exudation, hyperaemia, edema, the number of ulceration, burning sensation, and pain degrees) were assessed before treatment, at day 3 and 7 after treatment. Short-term efficacy was assessed by visual analogue scale (VAS). The total paralysis time and the total number of ulceration at month 12 after treatment were taken as judgment for long-term efficacy. Results Compared with before treatment in the same group, symptoms and signs were obviously improved at day 3 and 7 after treatment in the two groups (P < 0.05). Compared with the control group at day 3 after treatment, the improvement of edema, exudation, pain degree, and burning sensation was more obvious in the experiment group (P < 0.05, P < 0.01). The improvement of edema, pain degree, and burning sensation at day 7 after treatment was more obvious in the experiment group than in the control group (P < 0.05). As for short-term efficacy, the total effective rate was 86.67% (52/60 cases) in the experiment group and 83.33% (50/60 cases) in the control group, with no statistical difference between the two groups (P > 0.05). As for long-term efficacy, the total effective rate was 90.00% (54/60 cases) in the experiment group, significantly higher than that of the control group with statistical difference [81.67% (49/60 cases), P < 0.05]. At month 12 after treatment, the total number of ulceration was reduced and the paralysis time of ulcer attack prolonged in the experiment group, with statistical difference when compared with the control group (P < 0.05).

CONCLUSIONQKJG showed better long-term efficacy than that of KG in treating recurrent oral ulceration (YDFET).

Chronic Disease ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Pain Measurement ; Phytotherapy ; Treatment Outcome ; Yin Deficiency ; drug therapy

Objective:To detect the expression of ALDH1 and ABCG2 in the specimens from different levels of cervical lesions.Methods:Use SP immunohistochemistry to detect the expression of ALDH1 and ABCG2 in 76 cases of cervical squamous cancers,52 cases of HSIL,and 37 cases of chronic cervicitis tissues,and analyze the results.Results:①The positive expression rates of ALDH1 and ABCG2 in chronic cervicitis tissues were 5.41％ (2/37) and 8.11％ (3/37),in HSIL tissues were 23.08％ (12/52) and 28.85％ (15/52),and in cervical cancers tissues were 53.95％ (41/76) and 61.84％ (47/76),respectively.The positive expression rates in cervical cancers tissues were significantly higher than in HSIL and Cc hronic cervicitis tissues,(P ＜ 0.05).②The positive expression of ALDH1 in cervical cancer tissues had correlation with tumor grade and lymphnode metastasis (P ＜0.05),while the positive expression of ABCG2 in cervical cancer tissues had correlation with tumor grade,clinical stage and lymph-node metastasis(P ＜0.05).③The expressions of ALDH1 and ARCG2 in cervical cancer were positive relative(r =0.535,P ＜ 0.05).Conclusions:The increased expressions of ALDH1 and ABCG2 in cervical squamous cancer may play a role in the occurrence,development and metastasis of cervical squamous cell carcinoma.ALDH1 and ABCG2 may have synergistic effect in occurrence of cervical cancer.

With the development of society and the improvement of people's living standards, the effect of Chinese medicine in treatment and health care is more and more prominent. The herbal decoction pieces are the important part of Chinese medicine,it can be applied directly to clinical treatment and it's also the raw material of Chinese patent medicine. Therefore, the quality of herbal decoction pieces is quite important. The parts of the production of herbal decoction pieces are numerous, and there are possibilities of adverse effects on the quality of the herbal decoction pieces in every part. In this paper, we based on the production chain of herbal decoction pieces, analyzed the main problem that affect the quality of herbal decoction pieces in the part of selection of Chinese herbal medicines, planting, purchasing, processing, packaging, storage and transport, such as the poor quality of seed and seedlings of plant-based Chinese medicines, some plants left their place of origin and have been introduced in the place that is not suitable for this kind of plant, the insufficient growth time and the excessive harmful substances. The purchasers and the accepters lack of professional knowledge and professional ethics. The mechanism of processing is not clear, the standards can not be uniformed, and lack of qualified person in processing, etc. So we suggest: intensify the basic research of key scientific issues. Improve the quality of persons who work in herbal decoction pieces; Establish an "integration" mode of operation in herbal decoction pieces enterprise; Breeding high quality plant resources, establish the large-scale planting basement; Make the packing of herbal decoction pieces standard; Establish the modernization traditional Chinese medicine logistics enterprise.
Chemistry, Pharmaceutical ; economics ; manpower ; standards ; Drug Packaging ; economics ; manpower ; standards ; Drug Storage ; economics ; standards ; Drugs, Chinese Herbal ; chemistry ; standards ; Humans ; Medicine, Chinese Traditional ; economics ; standards ; Quality Control

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Longitudinal Studies ; Male ; Neoplasm Staging ; Neuroblastoma ; diagnosis ; pathology ; therapy ; Treatment Outcome

Objective To detect the proliferation of neural stem cells (NSC) and analyze the effects of taurine on NSC proliferation in rats with fetal growth restriction (FGR).Methods For in vivo experiment,pregnant rats were randomly divided into three groups:control group (n=5),FGR group (n=7) and taurine group (n=6) [treated with taurine,300 mg/(kg · d)].Rats in the latter two groups were fed with 40％ of food intake in control group throughout pregnancy to establish the FGR model.Fatty acid binding protein-7 (FABP-7) expression in brains was detected by immunohistochemistry.For in vitro experiment,pregnant rats were divided into two groups including control group and FGR group.FGR modeling was established in the same way as above.On the 14.5 day of pregnancy,NSC were isolated and cultured for five days to form neurospheres with different concentrations of taurine (control,0,5,10,15,20,30,40 mmol/L).Immunofluorescence staining was used to detect the expression of NSC-specific marker FABP-7.CCK8 test was used to detect the cell proliferating index.Neurospheres were collected at the end of culture and disaggregated to get the total number of viable cells.One-way ANOVA,Kruskal-Wallis Test,SNK-test and Tamhane's test were used for statistical analysis.Results Compared with the control group (18 012±3 016),the number of FABP-7-positive cells in FGR model group (10 173±1 728) was decreased (P＜0.05),but increased in taurine group (19 518±3 201) (F=200.8,P＜0.05).Free-floating neurospheres could be formed in both control and FGR groups and the cells within neurospheres were mostly FABP-7-positive and were then considered as NSC.The proliferation indexes in control,FGR,FGR+5 mmol/L taurine,FGR+10 mmol/L taurine,FGR+15 mmol/L taurine,FGR+20 mmol/L taurine,FGR+30 mmol/L taurine,FGR+40 mmol/L taurine groups were 1,0.80±0.07,0.92±0.11,1.33±0.24,1.20±0.15,1.11 ±0.14,0.80±0.12 and 0.82±0.08,respectively.The proliferation indexes in FGR groups treated with 10,15 or 20 mmol/L of taurine were significantly higher than that of FGR group without taurine interference (P＜0.05).The total cell numbers in the eight groups were (24.5 ± 0.6)× 105,(20.23 ± 1.09)× 105,(22.80 ±0.86) × 105,(27.53± 1.22)× 10s,(25.30±1.05)× 105,(22.70±0.72)× 105,(21.80± 1.80)× 105 and (18.06 ± 0.90)× 105,respectively.Compared with the FGR group without taurine interference,those treated with 5,10,15 or 20 mmol/L of taurine showed higher total cell numbers (all P＜0.05 or ＜0.01),while that treated with 40 mmol/L oftaurine was lower.Conclusions The number and proliferation of NSC in FGR fetal rat brains is significantly decreased,however,these could be reversed by antenatal supplementation of taurine.The best effect is shown when l0 mmol/L of taurine is given.