AIM: To establish a method of simultaneous determining 4 components in Shengmai Injection (Radix et Rhizoma Ginseng Rubra,Radix Ophiopogonis,Fructus Schisandrae Chinensis). METHODS: Four components:ginsenoside Rg_1、ginsenoside Re、ginsenoside Rb_1 and schisandrin in Shengmai Injection were determined simultaneously by HPLC, using a waters symmetryshield TMRP_(18) column(4.6 mm?250 mm; 5.0 ?m),acetonitrile-water as a mobile phase. The detection wavelength was at 203nm. RESULTS: The relationship between the concentrations and the peak areas of ginsenoside Rg_1、ginsenoside Re、ginsenoside Rb_1 and schisandrin was linear respectively.The RSD of precision,repeatability and recovery were all less than 1%. CONCLUSION: The method is simultaneous determination for four components in Shengmai Injection,and can be applied to the quality control of Shengmai Injection.

With great theoretical and practical significance,training demand analysis is the prerequisite and basis for other training activities.This paper gives a brief introduction and analysis to modern training demand which featuring with diversity and multi-hierarchy.This paper analyzes the participants' needs and identifies the influence factors by using chi-square test and other methods to meet the needs of training programs.And we provide the detailed solutions for addressing those problems.

To set up a new method of detecting bacterial number in situ,NADH fluorescence method based on the fluorescent characteristic of NADH was used.When the concentration of NADH ranged from 10 nmol/L to 0.2 mmol/L,its concentration had a good line relationship to the fluorescence intensity(R2= 0.9905).Separating bacterial cells by centrifugation and extracting NADH with hot Tris-HCl buffer,the re-sult of bacterial count detected with NADH standard plot was 1?104 CFU/mL in an hour.In summary,NADH fluorescence method is rapid,sensitive,simple and reliable to detect total bacterial number.There-fore,the method can be widely applied in the field of food sanitation and safety,environment detection and so on.

Adolescent ; Electrocardiography ; Female ; Humans ; Myocarditis ; complications ; physiopathology ; Tachycardia, Ventricular ; etiology ; physiopathology

The production and the developing process , the technical principle and the characteristic of organism weak magnetic field analytic technology are elaborated, as well as the application on health maintenance testing and psychologi- cal testing in recent years. It is pointed that organism weak magnetic field analytic technology has high clinical reference value and the application value on some aspects, including sub-health state analysis, equilibrium state analysis of alimen- tation and metabolic balance, influencing analysis of heredity and environmental deleterious factors on health, analysis of tumor susceptibility, early warning and predict of body's potential disease and psychological condition examination.

Objective: Studying the affect of chronic stress to ultrastructure and the expression of vascular cell adherence molecules-1 in thoracic aorta in rats. Methods: 24 male Wistar rats are divided into two groups randomly, including the test group and control group. The number of each group is 12. The test group are compelled to receive a series of stimulation after they are fed 1 week in quiet surrounding, but the control group are not. All of rats are executed at the same time. After have drawn the materials from the thoracic aorta, we observe the ultrastructure by electronic microscope and the expression of vascular cell adherence molecules-1 by the method immunohistochemistry in thoracic aorta. Results: ①The ultrastructures in test group are changed, but the control group are normal; the modification is concentrated on the obvious swelling, vacuolicitical transformation in mitochondria, but the control are not. ②The VCAM-1 is highly expressed in thoracic aorta of the test group, there is not in control group(P

BACKGROUND: There is a complicated relation between somatic disease and mental disorder. Somatic disease may induce functional disorder of brain, and generate psychiatric symptoms due to the change of psychological and social environment. The proportion of depression in elder people based on the chronic somatic disease is much higher, and thus the fatality of them increases.OBJECTIVE: To investigate the influence of single antidepressant, and therapeutic alliance of psychological and drug treatment on the rehabilitation of patients with elderly chronic somatic disease complicated with depression. DESIGN: Randomized and controlled grouping experiment.SETTING: Department of Senile Disease of Tongji Hospital Affiliated to Huazhong University of Science and Technology.PARTICIPANTS: Totally 102 patients were hospitalized in the Department of Senile Disease of Tongji Hospital Affiliated to Huazhong University of Science and Technology from March 2000 to January 2003, and all the patients had sleep disorder to a different extent, and suffered from symptoms of heart and alimentary tract which could not be explained as organic diseases, and chronic pain or systemic symptom. Their score of Zung scale was ＞ 40 points with average of (48.83±4.15)points. The patients were randomly divided into the drug group (n=46) and the combined treatment group (n=56). Each patient had more than two somatic symptoms in the two groups and more than 81.0% of them had three somatic symptoms. METHODS: According to the different primary diseases in patients of the two groups, regular treatment was given routinely. Oral thymoleptics fluoxetine of 20 mg/d 5-hydroxyltryptamine reuptake restrainer with 20 mg/d was administered, the dose of which could be added to 40 mg/d according to the pathogenetic conditions. At the beginning of the administration of oral antidepressant drugs, the patients in the combined treatment group were The focal point of depression and fear was identified, and the comfort and edge related to the disease was supplied, and the pathogenetic condition and the prognosis were told to the patients objectively. The family and society were encouraged to learn the rehabilitative skill and improve their possible, and the family and society were encouraged to give the patients adequate psychological support and life support. The patient and the family were assisted to establish favorable communication. The feeling of loneliness and isolation was reduced, and the sense of safety was increased. The depression status and the quality of life of the patients in the two groups were evaluated before the treatment and at 2 and 8 weeks after treatment with the Zung scale and the Spitzer quality of life index score (QLI), including 5 aspects: Activity, daily life, health, support and perspective. The highest score was 10 points. The lowest was 0, and the higher the score, the better the quality of life is.MAIN OUTCOME MEASURES: The results of the Zung scale and the QLI score evaluated before the treatment and at 2 and 8 weeks after the treatment.RESULTS: The treatment of the patients in the two groups was all finZung scale in the drug group and the combined treatment group at 2 weeks after the treatment indicated that there was significant difference in comparison with those before the treatment (41.12±2.60,48.83±4.15,t =10.67,P ＜ 0.05); (40.88±3.43,49.03±4.21 ,t=11.24,P ＜ 0.05). There was not significant difference in group comparison. The total points of Zung scale at 8 weeks were significantly decreased (36.11 ±2.87 ), (30.71±3.62),and there was significant difference in comparison with that before the treatment. The combined treatment group was better than the drug group in difference of QLI score at 2 weeks between the drug group and combined treatment group before and after the treatment (5.47 ± 1.33,5.32 ± 1.04,5.55±1.18,5.27±1.35). The QLI score of drug group and combined treat ment group at 8 weeks after treatment began to rise 7.21±0.13 and 8.83 ±0.32 ,respectively, and there was significant difference in comparison withthose before the treatment, and there was also significant difference in group comparison.CONCLUSION: The positive and effective combined therapy of anti-depression significantly improved the somatic symptoms of patients, no complications appeared, and most patients presented optimistic attitude to the prognosis of the primary disease. To identify the depression of elder people in earlier period is very important to promote rehabilitation of the primary disease.

Objective To observe the change of homocysteine(Hcy)and lipoprotein-a[Lp(a)]of cerebral infarction patients and perform the correlation analysis on them.Methods The serum Hcy and Lp(a)levels were detected in 230 patients with cerebral in-farction and 90 healthy controls.The detection results were performed the comparison between the groups and the correlation analy-sis.Results The serum Hcy and Lp(a)levels in the overall cerebral infarction group and the cerebral infarction group with in-creased Hcy were significantly higher than those in the healthy controls(P <0.01).The serum Hcy and Lp(a)levels in the cerebral infarction group without increased serum Hcy had no statistical difference compared with the healthy control group(P >0.05).The serum Hcy and Lp(a)levels in the overall cerebral infarction group had no statistical difference compared with the cerebral infarc-tion group with increased Hcy(P >0.05).The serum Hcy and Lp(a)levels in the overall cerebral infarction group and the cerebral infarction group with increased Hcy had statistical difference compared with the cerebral infarction group without increased Hcy (P <0.01).The serum Hcy in the overall cerebral infarction group is positively correlated with Lp(a)(r =0.859,P <0.01).Con-clusion The increase of serum Hcy and Lp(a)levels is closely related with the occurrence of cerebral infarction and has certain val-ue in the diagnosis of cerebral infarction,moreover serum Hcy is positively correlated with Lp(a).

Objective To explore the efficacy of endoscopic electric cutters in the treatment of adenoidal hypertrophy .Methods 36 patients who underwent adenoidectomy treatment of adenoid hypertrophy were divided into the observation group and control group according to the random number table .The observation group was taken endo-scopic electric cutters treatment , the control group was taken traditional adenoid removal treatment .The therapeutic effect of the two groups was compared .Results In the observation group,the total time required for removal of the adenoids was (10.65 ±2.38)min,blood loss was (28.82 ±0.98)mL,compared with the control group,there were significant differences(t=25.93,31.79,χ2 =3.92,all P<0.05).In the observation group,there was 1 case had severe pain,but in the control group,there were 9 cases,the difference was significant (χ2 =8.86,P<0.05).In the observation group,the total effective rate was 94.45%,which was significantly higher than 55.55% in the control group(χ2 =7.26,P<0.05).Conclusion Compared to the traditional treatment of adenoid surgery ,electric cutters endoscopic treatment can significantly reduce adenoidal hypertrophy blood loss and the incidence rate of complication , improve patients'pain and clinical outcomes ,it is worth clinically further promotion .

Objective To detect serum leucine aminopeptidase (LAP) level in the patients with hyperthyroidism for investiga‐ting the changes of LAP in the patients with thyroid dysfunction and its clinical significance .Methods 117 patients with hyperthy‐roidism were taken as the test group and 109 healthy people as the control group .The differences of various laboratory indexes were comparatively analyzed and the correlation between LAP with FT 3 ,FT4 and TSH was analyzed .117 patients with hyperthyroidism were divided into the liver damage group(52 cases) and non‐liver damage group(65 cases) and performed the LAP determination . Results The serum LAP ,FT3 ,FT4 and TSH levels in the hyperthyroidism whole group ,hyperthyroidism liver damage group and hyperthyroidism non‐liver damage group had statistically significant differences compared with those in the control group (P<0 .01) ,serum LAP was positively correlated with FT 3 and FT4 ,and negatively correlated with TSH ;LAP ,FT3 ,FT4 and TSH had no statistical differences between the hyperthyroidism live damage group and the hyperthyroidism non‐liver damage group (P>0 .05) .Conclusion The serum LAP content is closely related with the thyroid hormones and has certain clinical reference value for the diagnosis ,disease condition evaluation and medication .Lap can serve as an independent diagnostic indicator .