Objective To establish mice modle of allergic rhinitis(AR) and to study the role of Staphylococcal enterotoxin B(SEB) and ovalbumin(OVA) in the modle.Methods Forty Balb/c mice were evenly randomized into OVA group,SEB group,OVA+SEB group and normal sodium group and AR modle was established.The symptom scores,total serum IgE concentration,IL-4 concentration were analyzed by factorial design.Meanwhile,the morphology change of nasal mucosa was observed.Results The symptom scores in OVA group,SEB group,OVA+SEB group and normal sodium group were 6.80?1.03,0.90?0.99,0.70?0.82,0.60?0.70 respectirely.The interaction of OVA and SEB had statistical significance(P

OBJECTIVE To establish mice model of allergic rhinitis(AR) and study the role of Staphylococcal enterotoxin B(SEB)and ovalbumin(OVA)in the model,and investigate the change of regulatory T cell(Treg)in the nasal mucosa of mice.METHODS Forty Balb/c mice were randomized into OVA group(A),SEB group(B),OVA+SEB group(C)and normal solution group(D).AR model was established.The symptom scores and count of Foxp3 positive cells in nasal mucosa were analyzed by factorial design.RESULTS The symptom scores in Group A,B,C,D were 0.90?0.99,0.70?0.82,6.80?1.03,0.60?0.70 respectively.Group C was successfully established as AR model.OVA and SEB had interaction(P

Uterine natural killer cells (uNK) are distinct from peripheral blood NK (pbNK) cells,which constitute up to 70％ of the decidual leukocyte population in the first half of pregnancy,and are considered to have a cytokine-secreting role rather than cytotoxic function.It plays an important role in trophoblast invasion and uterine spiral artery remodeling.As a result of imbalance of uNK cells,pathological pregnancy appeared.Herein,the origin of uNK cells and its function in pregnancy were veviewed.

Objective　To study the effect on the proliferation of vascular smooth muscle cells(VSMC) after transferring angiotensin Ⅱ(AngⅡ) type 2 receptor(AT2R) gene.Methods　The recombinant adenoviral vector AdcCMV-AT2R, containing rat AT2 receptor gene which was constracted by homologous recombination, was used to transfer AT2 receptor gene to rat VSMC in vitro. The expression of AT2R mRNA was detected by RT-PCR. The rate of expression and the change of cell cycle in VSMC were analysed by flow cytometry. Cell devision index, incorporation of bromodeoxyuridine(BrdU)and 3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenytetrazolium bromide(MTT) were used to determine the proliferation of VSMC, respectively.Results　RT-PCR showed that the expression of AT2R mRNA increased obviously in transferred VSMC, and the peak value of expression rate was about 89.51% at 48 hours. When the expression of AT2R was at peak value, the ratio of S and G2-M periods was reduced from 31.7% to 13.9%(P<0.05) and the index of cell division from 37.4% to 9.6%(P<0.01). The OD values of MTT and BrdU incorporation were reduced by 61.4% and 51.6% respectively(P<0.01).Conclusion　Our study indicates that AdCMV-AT2R can generate high level expression of AT2 receptor in cultured rat VSMC and its expression can significantly inhibit the proliferation of rats VSMC in vitro. It may be a new selection for the gene therapy of restenosis after angioplasty.

Objective To evaluate the changes of left atrial(LA)function in peripartum cardiomyopathy(PPCM)patients using two?dimensional speckle tracking echocardiography(2DSTE). Methods Totally 35 PPCM patients and 35 healthy postpartum women(control group)were en?rolled in this study. Left ventricular end?diastolic diameter(LVEDd)and LA anteroposterior dimension(LAAD)were measured. The end?diastol?ic volume(EDV)and end?systolic volume(ESV)were obtained using biplane modified Simpson′s method. Cardiac output(CO)and left ventricu?lar ejection fraction(LVEF)were also calculated. E wave and A wave of mitral valve were measured,and correspondingly E/A ratio were obtained. LA longitudinal systolic strain(SS),systolic strain rate(s?SR),early diastolic strain rate(e?SR),and late diastolic strain rate(a?SR)were ob?tained by 2DSTE. Results There was no statistical difference of E wave between the two groups(P>0.05). Compared to the normal control group, LVEDd,EDV,ESV,LAAD,E/A were increased,while CO,LVEF,A,SS,s?SR,e?SR,a?SR were decreased in the PPCM group(P<0.05). a?SR was positively correlated with A wave in patients with PPCM(r=0.775,P=0.001). Conclusion LA reservoir,conduit and booster pump func?tion were decreased during PPCM. 2DSTE can easily and accurately assess these changes of LA function.

Objective Based on dual channel melting curve analysis-based assay,we developed a method to rapidly detect the drug-resistant mutations in Mycobacterium tuberculosis through real-time PCR.Methods According to the common first-line drug-resistant mutations of Mycobacterium tuberculosis,we designed six dual-labeled fluorescence probes to rapidly detect the drug-resistant mutations through realtime PCR melting curve after amplifications of drug-resistant related gene region of DNA.The targets include rpoB 81 bp core region,katG315,inhA promoter,ahpC promoter and embB306.To validate the sensitivity and specificity of our method,we performed real-time PCR assays to detect drug-resistant mutations in 76 clinical MDR-TB samples,which were collected by Shanghai CDC in 2008.Results In the validation,this method successfully detected drug-resistant mutations in all 76 clinical MDR-TB samples.The △Tm of mutations were from 1.8 to 14.4 ℃.Comparing with the sequencing data,all mutations covered by the six probes were detected with 100％ sensitivity and 100％ specificity (rpoB,80/80; inhA,7/7 ; katG315,59/ 59;ahpC,8/8;embB306,27/27).This method can successfully detect drug-resistant mutations from 100 copies/μl DNA samples.Conclusions A widely applicable real-time PCR assay to detect first line drug-resistant mutations of Mycobacterium tuberculosis has benn developed.This method has proven to have the advantages of high sensitivity,specificity and low risk of contamination.It can be used in rapid diagnosis of clinical drug-resistant tuberculosis and the evaluation of laboratory drug sensitivity test.

Maternity insurance is a policy support of China for women of child-bearing age.Given its good outcomes since it is enforced clinically,such insurance has been confronted with challenges as well.Doctors and nurses of Ob/Gyn departments serve as both protectors of parturient women's interests and practioners of the economic and social interests of the hospital.This paper summarized the experiences of maternity insurance of the Ob/Gyn department of the hiospital in rencent 6 years.The hospital has had 2026 inpationts covered by maternity insurance,without any medical disputes and harvesting a cash surplus of 657145 yuan.Such doctors and nurses are found to play a key role in ensuring a good doctor-patient relationship,and serve as clinival references for the single-disease management and teaching of the hospital.

Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.

Objective To study the anatomical characteristic of nanophthalmos with ultrasonography and its clinical significance. Methods Forty-four nanophthalmic eyes (22 patients) and 200 normal eyes (100 persons) were included. A series of ocular parameters, including ocular axial length, angle between trabeculum and iris,thickness of lens and sclera.and vitreous cavity length were measured with ultrasound A-scan and ultrasound biomicroscopy. The refractive status was examined with dioptometry. Results The ocular axial length[(15. 95±0. 76)mm] and vitreous cavity length [(8. 48±0. 90)mm] were shorter, the lens [(4. 9±0. 53)mm] and sclera [(0. 917±0. 119)mm] were thicker in nanophthalmic eyes than those of normal eyes( P <0.01). The relative position of lens (0.312±0.09) was more forward, and the angle between trabeculum and iris (11. 41 ±7. 63 ) was narrower in nanophthalmic eyes, compared with normal eyes( P <0. 01). The hyperopia of nanophthalmic eyes was negatively correlated with the ocular axial length ( r = -0.806) and vitreous cavity length ( r = - 0. 663) ( P <0. 01). Conclusions Nanophthalmos was characterized by short ocular axial length, thick lens and sclera.and narrow anterior chamber angle. The thickness of sclera and the angle between trabeculum and iris measured with ultrasound biomicroscopy could provide a solid indication of the treatment to the complication of angle closure glaucoma.

Objective To investigate the correlation between high myopia and MMP-2 in aqueous humor (AH), by comparing the protein level of MMP-2 in AH from patients with cataract combined with high myopia and with emmetropia. Design Experimental study. Participants 30 AH samples of patients with age-related cataract. Methods AH samples were collected from the patients with age-related cataract during phacoemulsification, including 15 AH samples from patients combined with high myopia (experimental group) and 15 AH samples from patients with emmetropia (control group). The expression of pro-MMP-2 and MMP-2 in AH were ana-lyzed by Western blot technique. Main Outcome Measures The gray value of MMP-2 protein band detected by Western blot. Results The level of pro MMP-2 was statistically higher than that of MMP-2 in experimental group (430.4±57.3 versus 294.5±35.2, t=10.400, P= 0.000) and control group (402.8±57.7 versus 280.3±49.7, t=8.400, P=0.000). There was no statistically difference in the level of pro-MMP-2(t=1.320, P=0.200)and MMP-2 (t=0.900,P=0.375) between the two groups. Conclusions No abnormal expression of MMP-2 was detected in AH from patients with high myopia, according to this study based on limited samples. Pro-MMP-2 was the main form of MMP-2 in AH of patients with cataract combined with high myopia or emmetropia, which possessed potential ability of transferring into form of active MMP-2. (Ophthalmol CHN, 2009, 18: 260-264)