Objective To investigate whether and how regulatory T cells(Tr) affect macrophages foam-cell formation, and thereby investigate the mechanism of Tr in the development of atherosclerosis. Methods Tr were isolated from lymphocyte suspensions by magnetic cell sorting-column and analyzed by flow cytometry. Macrophages were cultured alone, with CD4~+ CD25~+ T, or CD4~+ CD25~- T cells in the pres-ence of oxLDL for 48 h to transform macrophages into foam cells. Oil red O staining and cellular lipid meas-urement were used to identify macrophage foam cell formation. Semi-quantitative PCR, quantitative real-time PCR and Western blot analysis were carried out to explore the mechanism of Tr-mediated suppression on macrophages foam cell formation. Results Foam cell formation, as identified by oil red O staining, was readily apparent in cells treated with CD4~+ CD25~- T cells and without T cells. After treatment with Tr, a marked decrease(13.9% ± 5.6% ) in foam cell count was observed, compared with untreated cells(13.9% ±5.6% vs 52.9% ± 10.4%, P<0.001 ) or CD4~+ CD25~- T-treated cells(13.9% ± 5.6% vs 53. 1% ± 17.2%, P<0.001 ), 52.9% ± 10.4% and 53.1% ± 17.2%, respectively (P<0.001). The similar effect of Tr was obtained when extracted oil red O and measured by a spectrophotometer. Cholesteryl ester ac-cumulation also used to quantify foam cell formation. Compared with untreated (no T) and CD4~+ CD25~- Tr-treated macrophage cells (CD25~-), the lipids accumulation in CD4~+ CD25~+ Tr-treated macrophage foam cells(CD25~+) was significantly reduced. Total cellular cholesterol and cellular cholesteryl ester was siginifi-cantly reduced in CD25~+ cultures relative to no T[TC(total cholesterol): 57.46 ± 17.92 vs 159.48 ± 16.38, P<0.01 ; CE(esterified cholesterol): 26.68 ± 8.88 vs 102.54 ± 16.67, P<0.001] or CD25~- (TC: 58.50±7.00 vs 150.55±25.11, P<0.01; CE: 26.68±8.88 vs96.90 ± 11.95, P<0.001) cul-tures. Moreover, PCR and Western blot analysis showed that the expression of both CD36 and SRA in Tr-treated macrophage foam cells was significantly down-regulated. Conclusion Results collectively suggest that CD4~+ CD25~+ Tr cells may inhibit macrophage foam-cell formation, which is largely attributed to a down-regulated expression in scavenger receptor in Tr-treated macrophage foam cells.

Objective:Peripherally inserted central catheter(PICC) has been application in clinical for many years,but the training in the knowledge about maintenance wasn't univesal systematically yet.To analyze the existed problems on the clinical peripherally inserted central catheter in washpipe technology and find out countermeasures. Methods: The self-made questionnaire was used to survey a total of 46 nurses in every department and make on-site operation demonstration. Results: Only 12 nurses' operation was completely correct and the correct rate was 26.1percent,which was related to the training,manager's attention,nurses' participate awareness. Conclusion: The training on nurses' operation of red tube needed to be strengthen.

Through the comparative analysis of traditional medical dispute handling methods and the concept of the third party mediation in medical dispute,this paper discussed that the third party mediation method in medical dispute obtain the recognition of both doctor and patient with its advantages such as neutrality,impartiality,convenience,and it had good running effect in the practice from place to place.But there still remain Some Problems,in cluding the intervention lacks initiative;the amount of indemnity lacks scientificity;the conciliation agreementlacks coercive enforcement;the privacy lacks protection;the institution development is limited.So we must improve the third party mediation work mechanism,identification process,operation mechanism and the relevant insurance system.

Objective To use peer education to guide community diabetic patients to adopt diet therapy using food exchange method in order to explore effective method for diet therapy of diabetes mellitus. Meth-ods 91 diabetic patients were selected using simple sampling method and randomly divided into the peer ed-ucation(45 cases) and the common education group(46 cases), the two groups adopted food exchange method for diet therapy for 1 year. The blood glucose, blood lipid, glycosylated hemoglobin and body weight index were compared between the two groups using t test. Results The blood glucose, blood lipid, glycosylated hemoglobin and body weight index all decreased in the two groups after intervention, but the the decreased de-gree in the peer education group was more obvious. The fasting blood glucose, 2 hour postprandial blood glu-cose, glycosylated hemoglobin, triglyceride, low-donordensity lipid protein and body weight index in the peer education were lower thanthose of the common education group, but HDL-C was higher. Conclusions Peer education is more effective in health education of food exchange method.

Objective To analyzer the clinical significance of the Xpert MTB/RIF method for rapidly detecting Mycobacterium tuberculosis(MTB) and rifampicin resistance in clinic.Methods A total of 552 sputum samples were collected from the patients in the Huizhou Municipal Institute of Tuberculosis Prevention and Control and tuberculosis departments in 5 counties and districts from January 2015 to December 2015.Smear microscopic examination ,Xpert MTB/RIF nucleic acid detection and improved Roche culture were performed.The samples of culture positive were performed the drug susceptibility test.The sensitivity ,specificity of various methods for detecting MTB were evaluated.The results of conventional susceptibility test and Xpert MTB/RIF for detec-ting rifampicin resistance were compared and analyzed.Results Compared with the Roche culture method ,the sensitivity and speci-ficity of Xpert MTB/RIF for detecting MTB were 95.3% and 93.1%.In the comparison of drug resistance detection results ,the sensitivity and specificity of rifampicin resistance in all positive samples were 82.1% and 97.8%.In the consistency analysis of Xpert MTB/RIF and improved Roche culture for detecting MTB classification ,kappa=0.688 ,indicating that the results of the two methods had good consistency.Conclusion The Xpert MTB/RIF method can be used in MTB early rapid detection and screening of rifampicin resistance detection ,which is conducive to clinical rapid decision.

The realization of symptom measurement of the traditional Chinese medicine is a must for the development of traditional Chinese medicine, which obtained a common acceptation academically. This article believed the methods for realizing symptom measurement are the combination of both TCM two basic theories of "the wholism conception" and "treatment based on syndrome differentiation" and modern scientific measures and methods. Meanwhile, the western medicine should be simply excluded, other than simply compare a TCM syndrome to a physiological and biochemical indicator of western medicine.

Female ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Hypothermia ; diagnosis ; Male ; Reference Standards ; Skin Temperature ; physiology ; Young Adult

Acupuncture Therapy ; Adolescent ; Craniocerebral Trauma ; complications ; Humans ; Male ; Optic Nerve Injuries ; etiology ; physiopathology ; therapy ; Vision, Ocular

To maintain the competitive superiority,the medical english majors should optimize and construct the characteristic curriculum system in order to cultivate high-quality professionals of medical english.Taking the medical english major of Beijing University of Chinese Medicine for an example,this paper discusses the theoretic consideration and practice in constructing the characteristic ‘trinity’ curriculum system of medical english majors,hoping that more attention can be paid to its further development.

Objective To compare the clinical-pathology features between male breast cancer ( MBC) and female breast cancer(FBC)patients.Methods Clinical data of 37 MBC and 300 FBC patients admitted in the First Affiliated Hospital of Zhengzhou University from Jun .2003 to Jun.2013 was retrospectively reviewed . Results The ratio of MBC to FBC was 54:10000.The age of MBC patients ranged from 39 to 80 years with the median age of 62 years old, older than FBC patients(t=3.117, P=0.002).The percentage of invasive ducal carcinoma was much higher in MBC patients than in FBC patients (χ2 =7.535,P=0.023).MBC patients in clinically stage III and IV were more than FBC patients (χ2 =16.471,P=0.000).The positive rate of estrogen receptor(ER)was significantly higher in MBC patients than in FBC patients (χ2 =6.294,P=0.043).The posi-tive rate of human epidermal growth factor receptor-2 ( HER2 ) was lower in MBC patients than in FBC patients (χ2 =11.222,P=0.004).The main surgical method of MBC was modified radical mastectomy , and there was no breast-conserving surgery for MBC, while the surgical method of FBC was various (χ2 =7.720,P=0.021). Conclusion There are significant differences between FBC and MBC patients in clinicopathological features , surgical method and even prognosis , which were important to guide the treatment of MBC and FBC .