AIM: To observe the pathological role of 3-nitrotynosine (3-NT) on Escherichia coli LPS-induced vascular hyporeactivity in rats and the therapeutic effect of antioxidants. METHODS: Forty male SD rats weighting from 200 g to 250 g were randomly divided into four groups: the control group (n=10); LPS shock group (n=10); uric acid-treated group (n=10); melatonin-treated group (n=10). 6 h after LPS shock, phenylephrine (0.5-2.5 ?g?kg -1) was applied intravenously to all groups and the percentage increase in MAP was detected, respectively. The concentration-response curve of aorta rings from all groups rats were obtained by cumulative addition of phenylephrine (PE), and PE E_ max, EC_ 50 were calculated. The concentrations of plasma malondialdehyde (MDA), nitrate/nitrite and 3-NT were assayed in all groups 6 h after LPS shock. RESULTS: The MAP level induced by PE significantly decreased to 54.60% in LPS shock rats compared with the control (P

Stathmin is a novel member of microtubule-destabilizing proteins that play a critical role in the regulation of the dynamic equilibrium of microtubules during different phases of the cell cycle.The overexpression of stathmin was found in different type of cancer.Inhibition of stathmin expression in malignant cells may interfere with their orderly progression through the cell cycle.Overexpression of stathmin can affect the action of antimicrotuble drugs by markedly decreasing binding of paclitaxel,and increasing binding of Vinca alkaloids.In addition,stathmin provides an attractive molecular target for cancer therapy.It may be possible to combine adenovirus-mediated anti-stathmin ribozyme therapy with a chemotherapeutic agent such as taxol to obtain a more potent antiproliferative and antitumor effect.

Objective To investigate the effect of nitrotyrosine (3-NT) on the expression of ?1D adrenoceptor (?1D-AR) mRNA in cultured vascular smooth muscle cells (SMCS) .Methods SMCS were obtained from the tunica media of thoracic aorta of 1 month old SD rats and cultured in DMEM medium. The experiment consisted of two parts. In part Ⅰ SMCS were incubated with 0,1, 10, 100 or 200 ?mol?L-1 3-NT for 24 h and in part Ⅱ SMCS were incubated with 100 ?mol?L-1 3-NT forO,12, 24, 48 or 72 h. The total RNA was isolated by using Trizol reagent. The expression of ?1D-AR mRNA was determined by RT-PCR and agarose gel electrophoresis. Results In part I incubation with 1 and 10 ?mol?L- 3-NT for 24 h had no significant effect on the expression of ?1D-AR mRNA while incubation with 100 or 200 ?mol?L-1 3-NT for 24 h decreased the expression of ?1D-AR mRNA compared with 0?mol?L-1 3-NT (P

Objective To study the effect of propofol on vascular reactivity and plasma malondialdehyde (MDA) and nitric oxide (NO) in septic shock.Methods Forty male SD rats weighing 200-250 g were randomly divided into 4 groups (n = 10 in each group) : group Ⅰ control; group Ⅱ septic shock; group Ⅲ septic shock + propofol and group Ⅳ septic shock + melatonin. The animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1. The femoral artery and vein were connulated for MAP monitoring and drug administration. The animals were breathing spontaneously. Septic shock was induced by intravenous LPS 15 mg?kg-1 . In group Ⅲ a bolus of propofol 10 mg?kg-1 was given i.v. at 1 h after intravenous LPS followed by intravenous propofol infusion at 10 mg?kg-1?h-1. In group Ⅳ melatonin 10 mg was given intraperitoneally at 1h after LPS i.v. . Six hours after LPS administration 4 doses of phenylephrine (PE) 0.5, 1, 2, 2.5 ?g?kg-1 were given i.v. in succession. The next dose was given when MAP returned to the baseline level after previous PE. The percent change in MAP after each dose was recorded. Blood samples were taken at 6 h after LPS administration for determination of plasma MDA and NO concentrations. After the in vivo experiment the animals were sacrificed and thoracic aortas were removed and cut into segments of 3 mm in length which were bathed in Krebs buffer aerated with 95% O2 and 5% CO2 at 37℃. The aortic rings were stretched to a resting tension of 2.0 g. The segments were then exposed to increasing concentrations of PE (from 1 nmol?L-1 to 30 ?mol?L-1). The dose-response curves were obtained. Emax and EC50 were calculated. Results The percent increase in MAP induced by PE was significantly reduced by septic shock (groupⅡ,Ⅲ,Ⅳ) as compared with control group (Ⅰ), but was significantly larger in propofol and melatonin groups (Ⅲ,Ⅳ) than in group Ⅱ. In the in vitro experiment the maximum response to PE and EC50 were significantly reduced in rats with septic shock as compared with rats in control group (P

] Objective To investigate the effects of midazolam on vascular capacitance during hypovolemic shock in an animal model and the underlying mechanism. Methods Thirty male SD rats weighing 350-450 g were randomly divided into four groups : (1) control group (n = 8);(2) sympathetic nervous system block ( SNSB) group received hexamethonium 10 mg? kg-1(n = 8);(3) SNSB + noradrenaline (NA) group in which NA was infused following hexamethonium until BP returned to baseline (n = 9); (4) hypovolemia group in which 20 ml? kg-1 of blood was withdrawn ( n = 5). The animals were anesthetized with intraperitoneal ketamine. Spontaneous breathing was maintained. Femoral artery and vein were cannulated for MAP and CVP monitoring. Mean circulatory filling pressure (Pmcf) was measured. Midazolam 0.1, 0.3, 0.5 and 1.0mg?kg-1 were given iv successively with an interval of at least 20 min between two injections. MAP, CVP, HR and Pmcf were measured and recorded before and 2 min after each midazolam injection. Results In control, SNSB + NA and hypovolemia groups (group 1, 3, 4) midazolam significantly decreased MAP as compared with that before midazolam ( P

Objective To analyze the correlation between fetal right aortic arch and chromosome ab-normality by ultrasound.Methods From January Jan 2009 to Dec 2014,nineteen cases with fetal right aortic arch were enrolled.They were all determined by chromosome karyotype analysis.The correlation of fetal right aortic arch and chromosome abnormalities detected by ultrasound were evaluated.Results Left lock,a “U”shaped vascular structure,were found in 15 cases.One case of fetal aortic arch was surrounded by a “O”shaped package.Three cases of right aortic arch showed fetal left artery catheter and brachiocephalic artery ima-ging branch.Echocardiography mainly for arterial catheter in three vessel trachea view did not show up.Artery catheter,in front of the trachea,did not form the vascular ring.Nineteen cases of right aortic arch were exam-ined by fetal karyotype analysis.Three patients with trisomy 18-karyotype presented ventricular septal defect, single artium,three tricuspid atresia,pulmonary artery stenosis.Four cases with trisomy 18-karyotype presen-ted ventricular septal defect,complete atrioventricular canal,single artium,double outlet right ventricle,pul-monary artery stenosis in atresia.Three cases with trisomy 21 -karyotype showed single ventricle,single atri-um.One case was with tetralogy of Fallot and one case with dextrocardia,aortic stenosis,accompanied by 22q 1 1.2.Conclusion In prenatal ultrasound screening,we should pay attention to three vessels-trachea view on ultrasound image,which can improve the detection rate of right aortic arch.Fetal right aortic arch and triso-my 18-,trisomy 21 -,chromosomal diseases are substantially correlated.Further analysis of chromosome kary-otype is needed to exclude chromosomal lesions,so as to achieve the purpose of prenatal and postnatal care.

Objective To detect the serum levels of carbohydrate antigen 199(CA199),carbohydrate antigen 125 (CA125)and carcino-embryonic antigen(CEA)in different population,and to explore their significances in diagnosis of pancreatic cancer.Methods The serum levels of CA199,CA125 and CEA in 56 patients with pancreatic cancer (pancreatic cancer group ), 5 6 patients with benign pancreatic disease (benign pancreatic disease group ) and 56 healthy volunteers(normal control group)were detected,then the positive rates of CA199,CA125 and CEA and the sensitivities and specificities of CA199,CEA and CA125 for the diagnosis of pancreatic cancer were compared among three groups.Results The levels of serum CA199,CA125 and CEA of the patients in pancreatic cancer group were significantly higher than those in benign pancreatic disease group and normal control group (P<0.05). The positive rates of CA199,CEA and CA125 in pancreatic cancer group were 87.50 %,71.42%,and 60.71%, respectively. The positive rates of the three tumor markers of the patients in pancreatic cancer group were obviously higher than those in other two groups (P<0.05).The sensitivity of CA199(87.50%)was the highest,while the specificity of CEA (9 1.9 6%)was the highest. The method of parallel combined testing increased the diagnostic sensitivity to 95.12% and negative predictive value to 90.47%,but its specificity and the positive predictive value were decreased. Serial combined examination increased the diagnostic specificity to 92.86% and the positive predictive value to 89.19%,but its sensitivity and negative predictive value were decreased.Conclusion Combined detection of serum levels of CA199,CA125 and CEA has higher sensitivity and specificity than single-marker detection in the diagnosis of pancreatic cancer.

The tumor microenvironment is closely related to the occurrence and development of tumor. Hypoxia is considered to be one of the most important factors in tumor microenvironment.Formation of hypoxic microenvironment can be found in most of malignant tumors,which can inhibit the anti-tumor immune response. Recent studies have indicated that immunosuppressive cells,tumor stem cells and circulating tumor cells in hypoxic tumor microenvironment can mediate immune suppression and immune tolerance,and then promote development of tumor.The new immune therapy will focus on normalizing tumor vasculature,reconstructing the tumor microenvironment,avoiding immune suppression and averting tumor immune tolerance.

Objective To investigate the role of NFIC on the stimulation effects of cAMP-induced differentiation of stem cells from the apical papilla ( SCAPs) in vitro. Methods SCAPs isolated from dental papilla of human imma-ture third molars were cultured by enzyme digestion. SCAPs were transfected with lentivirus that overexpressed NF-IC gene ( ov-NFIC) or an empty vector ( LV-empty) and co-treatment with Forskolin. Mineralized nodule formation of each group was measured by alizarin red staining. Quantitative real-time reverse-transcription polymerase chain reaction was performed to test the expressions of RUNX2,ALP,OCN mRNA. Results Forskolin increased the ex-pression of Runx2, ALP, OCN mRNA as well as matrix mineralization in SCAPs, and the stimulation effects of For-skolin were enhanced by overexpressing NFIC gene. Conclusion The results indicate that NFIC can promote cAMP-induced differentiation of SCAPs.

Objective To evaluate the operation of laparoscopic splenectomy(LS),its safety and clinical effects.Methods Literature of the advances of LS were reviewed and analyzed.Results With the development of technology and surgical technique,the indications for LS were widened,the size of spleen plays an important role in the clinical outcome of LS.Conclusion LS has all the advantages of minimally invasive surgery.The application of hand-assisted technique is safe and feasible for giant spleen.With the accumulation of surgical experiences and technique development,LS will be extensively used in clinic.