Heme oxygenase(HO) is a rate-limiting enzyme that catalyzes heme degradation.It catabolizes heme into 3 products: carbon monoxide(CO),free iron and bilirubin,which play an important role in keeping the steady state of the organism.In recent years,some studies show that the promotion of HO-1 expression can reduce ischemia-reperfusion injury of an organ transplant and regulate the transplantation immunological rejection.It has an important application prospect in liver transplantation.

Objectiv To explore the effects of chromium (Ⅵ) on the levels of malondialdehyde (MDA), the final product of lipid peroxidation in peripheral blood of mice and the micronucleus rate of polychromatic erythrocytes (PCE) in peripheral blood. Method 60 Kunming mice were divided randomly into 6 groups averagely. 4 groups were selected as exposure groups which were exposed to 10.27, 13.83, 20.74, 41.48 mg/kg calcium chromate by oral perfusion, one time per day, for 6 days. The negative and positive control group was exposed to distilled water and cyclophos phamide (40 mg/kg) respectively. After exposure, the levels of MDA and micronucleus rates of PCE were determined. Results Significant increases of the levels of MDA and the micronucleus rates of PCE in peripheral blood of mice were observed, which were associated with the increases of the exposure doses of chromium (Ⅵ). Significant positive correlation was observed between the micronucleus rates of PCEs (x) and MDA(nmol/ml) levels (y) in peripheral blood (y=3.705+930x,r=0.958, P

Objective To use peer education to guide community diabetic patients to adopt diet therapy using food exchange method in order to explore effective method for diet therapy of diabetes mellitus. Meth-ods 91 diabetic patients were selected using simple sampling method and randomly divided into the peer ed-ucation(45 cases) and the common education group(46 cases), the two groups adopted food exchange method for diet therapy for 1 year. The blood glucose, blood lipid, glycosylated hemoglobin and body weight index were compared between the two groups using t test. Results The blood glucose, blood lipid, glycosylated hemoglobin and body weight index all decreased in the two groups after intervention, but the the decreased de-gree in the peer education group was more obvious. The fasting blood glucose, 2 hour postprandial blood glu-cose, glycosylated hemoglobin, triglyceride, low-donordensity lipid protein and body weight index in the peer education were lower thanthose of the common education group, but HDL-C was higher. Conclusions Peer education is more effective in health education of food exchange method.

Objective To study into the influence of Ze Xie and Dan Shan root on the immunity regulation and the expression of radical in mice with chronic pelvic infection. Methods The model of mice with chronic pelvic infection was established by putting phenol into the endometrium of the mice. The density change of serum IL -2, TNF, SOD and MDA of the mice was measure by the method of radiation immunity and the method of biochemistry. Results The expression of serum IL - 2 and SOD in Ze Xie and Dan Shan root group was obviously higher than that in the model group( P

Objective To investigate the expression of cyclooxyenase 2 (COX 2)in carcinogensis and development of endometrium carcinoma Methods Immunostainings, westernblotting and quantitve reverse transcription polymerase chain reaction (RT PCR) assay were utilized to measure levels of protein and mRNA expression of COX 2 in following five study groups: 25 cases with proliferative phase, 25 cases with secretory phase,25 cases with endometritis, 23 cases with atypical proliferative phase and 34 cases with endometrium carcinoma Results COX 2 expression of both RNA and protein in patients with endometrial carcinoma was higher significantly than patients with proliferative phase, secretary phase,endometritis,atypical proliferate phase Immunostaining score was 5 46?0 12 vs 3 20?0 18,4 78?0 12,6 10?0 25,8 70?0 93, average absorbent value was 0 75?0 23 vs 0 41?0 45, 0 56?0 31, 1 10?0 56,1 46?0 41;concentration of mRNA [(93?8) vs (65?11),(79?6),(299?11),(493?30) fpg/?g respectively] Successively the expression of COX 2 in atypical proliferation group was higher than normal endometrial and endometritis group The expression in proliferative phase group was higher significantly than secretory phase group ( P

Purpose To investigate clinicopathological features,diagnosis and differential diagnosis of tubular carcinoma of the breast.Methods Morphological observation and immunohistochemistry were applied in 29 cases of tubular carcinoma of the breast, and the literatures were also reviewed.Results The tumors were composed of open tubules, infiltrating haphazardly in the cellular desmoplastic stroma. The neoplastic tubules were enclosed by a single layer of epithelial cells with insignificant atypia or rare mitoses.The tumors were often associated with lobular neoplasm, columnar cell lesion, ductal atypical hyperplasia or carcinoma in situ. Immunostaining displayed that myoepithelial cells were absent or incomplete.Conclusions Tubular carcinoma of the breast is a peculiar subtype of breast carcinoma with good prognoses, which is easily confused with microglandular adenosis, radial sclerosing lesions,or low grade invasive ductal carcinoma.Immunohistochemistry is useful for the differential diagnosis.

Complexity science is a new type of scientific research method, and the combination of complexity science, life science, and medicine is still in the initial stage of exploration, and domestic medi-cal colleges do not offer correlative course currently. In order to broaden students' vision, transform their traditional thinking model, and prepare the future of multidisciplinary cooperation, medical colleges should open complexity science and medicine correlative course for senior undergraduate and graduate students. Course content should start by the famous fablebutterfly infect, followed by the introduction of chaos and fractal, self-organization and complex system theory concept, theoretical framework, and their application in the medical field The teaching material should refer to some popular science books, such as Mystery of the butterfly effect. The teaching method should draw lessons from metaphor.

ObjectiveTo investigate the effects of HO-1 on mast cells degranulation during liver ischemia/reperfusion injury.MethodsSD rats ( n =20) were randomly divided into 4 groups : ( 1 ) sham operation group, (2) ischemia/reperfusion injury group (I/RI group), (3) CoPP group, CoPP was given before 24 h(5 mg/kg), (4) ZnPP group, ZnPP was given before 24 h (20 mg/kg). The rat model of liver ischemia/reperfusion was built. Samples were collected after 2 hours reperfusion. HO-1 mRNA expression was assessed by RT-PCR. The expression of HO-1 protein was assessed by Western blot. Serum ALT, AST levels were determined. The amount of degranulated mast cells were detected by toluidine blue staining. Liver injuries were evaluated by HE staining.ResultsCompared to sham group, liver HO-1mRNA and protein expression, ALT, AST levels, the number of degranulated mast cells significantly increased in I/RI group, CoPP group and ZnPP group. Moreover, liver injuries were more serious. Compared to I/RI group, there were increased HO-1mRNA and protein expression, reduced ALT, AST levels and the number of degranulated mast cells, also reduced liver injuries were found in CoPP group. Compared to I/RI group, the expression of HO-ImRNA and protein was down-regulated, ALT, AST levels elevated and the number of degranulatedmastcellsincreasedaccompanyingmoreseriousliverinjuriesinZnPPgroup.ConclusionsHO-1 overexpression reduces liver ischemia/reperfusion injury, possibly by inhibiting mast cells degranulation in liver tissues.

Objective To investigate the dynamic changes of endoplasmic reticulum stress-related molecules including glucose regulated protein (GRP78), C/EBP homologous protein (CHOP), and caspase-12 in sciatic nerve of diabetic rats and explore its mechanisms.Methods Rats were randomly divided into normal control group (NC) and diabetes mellitus group (DM) that were induced by intraperitoneal injection of Streptozocin after 4 weeks of high-fat chow feeding.Sciatic nerves were isolated for three times at 4 weeks, 8 weeks and 12 weeks after induction of diabetes.The expressions of GRP78, CHOP,and caspase-12 were detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses.The morphology of sciatic nerve was investigated with electron microscope.Results With the extension of the course, demyelinating and axonal injury appeared in sciatic nerve of diabetic rats.The expressions of GRP78 mRNA and protein in DM group were significantly higher than NC group at 4 weeks and 8 weeks after induction of diabetes(P ＜0.05, P ＜0.01).The expressions of CHOP mRNA and protein in DM group were significantly higher than NC group at 8 weeks and 12 weeks after induction of diabetes (P ＜ 0.05).The expressions of caspase-12 mRNA and protein in DM group were significantly higher than NC group at 8 weeks after induction of diabetes(P ＜ 0.05, P ＜ 0.01).Conclusions Endoplasmic reticulum stress-related molecules (GRP78, CHOP, and caspase-12) contributed to the peripheral nerve injury of diabetic rats, and displayed dynamic changes.

Objective To discuss whether it is necessary to integrate metabolic indices into diagnosis of polycystic ovary syndrome (PCOS).Methods Taking ESHRE/ASRM diagnosis as the gold standard,51 women with PCOS and 47 women without PCOS were selected and divided into the intervention group and control group respectively from the Department of Gynecology in the Second Affiliated Hospital of Kunming Medical University between January 2013 and July 2014.Logistic regression based on principal component analysis and significant variables chosen through single factor analysis were used to establish the new diagnostic model which combined reproductive indices and metabolic indices.We evaluated the validity and reliability of the new diagnostic model by using ROC curve analysis.Finally,we analyzed the consistence and difference between the new diagnostic model and the gold standard.Results Thirteen significant variables were chosen using single factor analysis.ROC analysis showed that an area under the curve was 0.976 (P＜0.001) and the optimal cut-off point was 0.526 with a sensitivity of 96.08％,a specificity of 93.62％ and a consistency of93.88％.The new diagnostic model had superior validity and reliability.The two diagnostic methods had strong consistence (Kappa=0.877,P＜0.001) and no difference (x2=0.167,P=0.688).Conclusion Considering that the integration of metabolic indices does not change the diagnosis result,we come to a conclusion that it is unnecessary to integrate metabolic indices into diagnosis of PCOS.