OBJECTIVE:To optimize ultrasonic extraction technology for polysaccharide from Aconiti lateralis radix praepara-ta(ALRP). METHODS:Uniform design method was applied to investigate the effects of liquid material ratio,ultrasonic time and ultrasonic temperature on extraction rate of polysaccharide from ALRP. Verification test was conducted and compared with the re-sults of conventional decoction and boiling method. RESULTS:Optimized ultrasonic extraction technology was as follow as liquid material ratio of 10 mL/g,ultrasonic time of 34 min and ultrasonic temperature of 73 ℃. The polysaccharide extraction rate in veri-fication test was 19.05%(RSD=0.60%,n=3),relative error of the predicted value (19.44%) was 2.0%,while the extraction rate was higher than decoction and boiling method(16.42%). CONCLUSIONS:Optimized ultrasonic extraction technology is sim-ple,rapid and stable with high extraction rate,which is suitable for extracting polysaccharide from ALRP.

Objective:To investigate the distribution of abnormal eating behaviors among middle school girls in Beijing and the psychological factors having influence on these behaviors Methods: HDI and ESC-21 were used to investigate 636 female middle school students in Beijing Results: (1) According to the BMI of the subjects, 80 3% of the subjects answered that they had paid attention to their weight and stature (2) The mean BMI of the subjects were 19 38, which is in the normal range, but their ideal body mass index(IBMI)was lower than the normal standard (IBIM

Automatic Data Processing ; Humans ; Medicine, Chinese Traditional ; Pulse

Objective To evaluate the utility of carbapenem inactivation method (CIM) in detecting carbapenemase-producing Acinetobacter baumannii.Methods A total of 121 strains of A. baumannii were identified and subjected to antimicrobial susceptibility testing by VITEK compact. Carbapenem inactivation method (CIM) was applied to detect the carbapenemase in the A. baumannii strains. The OXA-23 type carbapenemase-encoding genes were analyzed by common PCR method.Results Six-eight of the 121 strains showed resistance to imipenem and meropenem. PCR showed that 65 of the 68 strains carried OXA-23 gene. CIM was positive in 66 of the 68 strains. And 52 of the 121A. baumannii strains were susceptible to imipenem and meropenem. PCR showed that OXA-23 gene was negative in 49 of the 52 strains. CIM was negative in the 52 strains of non-carbapenemase-producing A. baumannii. Only one strain was resistant to imipenem but susceptible to meropenem. CIM was negative but QXA-23 was positive for this strain. The sensitivity and the specificity of CIM was 94.2% and 98.1% respectively in detecting carbapenemase-producing A. baumannii.Conclusions The results of CIM were consistent with the results obtained by PCR to detect the encoding gene of OXA-23. CIM is inexpensive, easier to operate and interpret than PCR method. CIM is applicable to detect OXA-23 type carbapenemase rapidly inA. baumannii.

Apoptosis ; Breast Neoplasms ; metabolism ; pathology ; Female ; Humans ; MicroRNAs ; metabolism ; physiology ; Receptor, ErbB-2 ; metabolism ; Receptors, Androgen ; metabolism ; Receptors, Estrogen ; metabolism ; Receptors, Progesterone ; metabolism ; Signal Transduction

We have carried out experiment design and comment,and students write the reports of experiment design in patho- physilolgy teaching from the aspects of basic process of experiment research and basic factors,principle and meaning of experi- ment design.By way of the teaching reform,the major position of students in studying is established,students' ability to study in- dependently and acquire knowledge actively are well cultivated,their comprehensive quality are enhanced and the teachers con- struction is also promoted.

OBJECTIVETo explore the effect of 3-n-butylphthalide pretreatment on the delayed neuronal death(DND) and the expreesion of heat shock protein70 (HSP70) in rat hippocampus after ischemia/ reperfusion.

METHODSAll rats were randomly divided into sham group (n = 36), total cerebral ischemia (TCI) group (n = 36), butylphthalide (NBP) group (n = 6), NBP + TCI group( n = 36), quercetin + NBP + TCI group (n = 6), dimethyl sulfoxide (DMSO) + NBP + TCI group (n = 6). The model of total cerebral ischemia/reperfusion was established by blocking vertebral arteries and carotid arteries. In sham group, TCI group and NBP group, the animals were further divided into instantly, 6 h, 12 h, 1 d, 3 d, 5 d groups according to the time interval after sham operation or TCI. Histological changes of the hippocampus were evaluated using thionin staining under light microscope by determining the delayed neuronal death (DND) and the expression of HSP70 was assayed using immunohistochemistry.

RESULTSNBP pretreatment could reduce delayed neuronal death in CA1 of hippocampus induced by TCI-reperfusion injury in rats, and up-regulated the expression of HSP70 in CA1 hippocampus of brain ischemic/reperfusion for 5 days. Quercetin blocked the acquirement of the brain ischemic tolerance induced by NBP preconditioning.

CONCLUSION3-n-butylphthalide (NBP) prevents the neurons from ischemia/reperfusion injury through upregulating the expression of HSP70.

Animals ; Benzofurans ; pharmacology ; CA1 Region, Hippocampal ; cytology ; pathology ; Cell Death ; Cerebral Infarction ; drug therapy ; HSP70 Heat-Shock Proteins ; metabolism ; Ischemic Preconditioning ; Neurons ; cytology ; Rats ; Rats, Wistar ; Reperfusion Injury ; drug therapy

Objective To observe the clinical efficacy of acupuncture plus He-Ne laser irradiation in treating cervical spondylotic radiculopathy (CSR) and to provide evidence for optimizing the treatment protocol. Method Totally 120 CSR patients were randomized into group A, group B, and group C, 40 in each group. Group A was intervened by acupuncture plus He-Ne laser irradiation, group B was by acupuncture alone, and group C was by the laser irradiation alone. The CSR rating scale was adopted for observation before and after intervention, and the therapeutic efficacy was also evaluated.Result The CSR rating score was changed significantly in all of the three groups after intervention (P<0.05). The change of CSR rating score in group A was significantly different from that in both group B and C (P<0.05). The total effective rate was 97.5% in group A, versus 92.5% in group C and 80.0% in group C, and the total effective rate of group A was significantly different from that in both group B and C (P<0.05).Conclusion Acupuncture plus He-Ne laser irradiation is an effective method in treating CSR.

Objective To compare the clinical efficacy of through moxibustion combined with electric acupuncture versus electric acupuncture alone for treatment of patients with cervical spondylosis radiculopathy (CSR), so as to provide a basis for optimizing clinical treatment.Methods A prospective study was conducted. Sixty patients with CSR treated in the clinic of Acupuncture Department as outpatients or admitted into the department of the First Affiliated Hospital of Xinjiang Medical University as inpatients were enrolled, and according to the random number table, they were divided into through moxibustion combined with electric acupuncture treatment group (observation group) and conventional electric acupuncture treatment group (control group), 30 cases in each group. The main acupoints of the observation group were as follows: Fengchi, Dazhui, Tianzhu, Houxi, Cervical Jiaji points, Shenshu, Mingmen, in cases with significant neck and shoulder pain, supporting points were added such as Jianjing, Tianzong, in cases with severe arm and finger numbness and pain, Quchi, Hegu, Waiguan, and in cases with marked dizziness and headache, Baihui, Temple acupoints were added. The needle 0.30 mm × 40 mm in size was chosen to be applied, at bilateral neck Jiaji points, direct subcutaneous needling (piercing) was made, at Dazhui, the piercing of needle for 1 to 1.5 inches was performed (body inch), at Mingmen, reinforcing method was used for piercing, at Shenshu, 0.30 mm × 50 mm needle was chosen for piercing with reinforcing method, and at the rest acupoints, conventional neutral supplementation and drainage method of acupuncture was applied. After obtaining qi from needling, electric needle device was added, the current strength used for individual patient depended on his or her degree of tolerance, the needle retention being 20 minutes, and during the above process, at Dazhui and a set of neck Jiaji points, moxibustion with moxa was applied. The acupoints used in the control group were the same as those in the observation group except moxibustion, other procedures were similar to those in the observation group. In both groups, the therapy was taken once a day for consecutive 5 days in a week, constituting one therapeutic course, and after two courses, the changes of clinical symptom scores and clinical efficacies were observed.Results After treatment, the clinical symptom scores in two groups were significantly higher than those before treatment (control group: 15.68±2.01 vs. 7.10±1.87, observation group:18.13±1.69 vs. 7.92±2.14), and the degree of improvement in the observation group was more significant (P < 0.01). After treatment, the total effective rate in observation group was significantly higher than that in the control group [96.67% (29/30) vs. 83.33% (25/30),P < 0.05].Conclusion The clinical therapeutic effect of through moxibustion combined with electric acupuncture for treatment of patients with CSR is better than that of electric acupuncture alone, so there is certain feasibility.

Reduced graphene oxide-BiPO4 ( RGO-BiPO4 ) nanocomposite was synthesized successfully via a one-pot solvothermal method using graphene oxide and bismuth nitrate as precursors and glycerin as solvent at 200℃ for 1 h. The morphology and structure of as-prepared nanocomposite were characterized by SEM, TEM, XRD, XPS, SERS and UV-Visible spectrum. The photocatalytic activity of the nanocomposite was evaluated by the photodegradation of Rhodamine B ( RhB) dye under UV irradiation and it was found that RGO-BiPO4 nanocomposite possessed higher photocatalytic activity than that of pure BiPO4 . RhB could be decomposed 87. 5% within 2 h. Under the same conditions, only 45. 7% of the RhB dye could be decomposed by BiPO4 . The enhancement of photocatalytic activity could be attributed to the effective charge separation due to the electron-accepting and transporting properties of graphene.