Objective To establish a high sensitive spectrophotometry for determination of trace cadmium in the water. Methods A complicated ion-association complex of Cd(Ⅱ)-potassium iodide-malachite green was formed in the phosphate acid, and the addition of gelatine could enhance the sensitivity of the reaction.The maximum absorption of the ion-association complex was at 680 nm,the effect of experimental conditions such as the reagents concentration,the temperature and the influence of foreign matters were considered.Results In the optimum condition(6.0 ml of 40% potassium iodide-aseorbic acid solution,0.5 ml of 5.0 mol/L phosphate acid solution,0.5 ml of 0.5% gelatine solution,1.5 ml of 1.0?10~(-3)mol/L malachite green solution in a 25ml volumetric flask,diluted with water and mixed well and determined immediately),the linear regression equation was △A=0.011+ 0.957 c,r=0.998 5.Beer's law was obeyed in the range of 0.02 ?g/ml to 0.80 ?g/ml for Cd(Ⅱ)and the limit detection was 0.02 ?g/ ml.The composing ratio of the complex was MG:Cd:I=2:1:4,and its apparent molar absorptivity coefficient was 1.08?10~5 L/(mol? cm).The recovery rates of Cd(Ⅱ)were 97.0%-101.5%,RSDs were 1.36%-3.58%.Conclusion This method is sensitive,simple, rapid and is applicable to the determination of the trace Cd(Ⅱ)in water.

Objective: To evaluate the clinical effect of individualized enteral nutritional (EN) support in severe stroke patients.Methods: Totally 120 severe stroke patients with nutritional risk were divided into two groups according to single or double admission number.The 60 patients in the intervention group received an individualized EN with different formula and concentrations according to the clinical status,and supplemented trace elements as occasion required.In the routine group, the patients received EN with standard formula.The energy supply was 83-125 kJ· kg-1·d-1 in both groups.Blood albumin(Alb), pre-albumin(PA), hemoglobin(Hb) and vitamin C(VC) were examined before and after the 21-day nutritional support.The lung infection and gastrointestinal (GI) complications were observed, and the hospital stay and cost were recorded as well.Results: After 21 day''s nutrition support, the serum levels of Alb, PA and VC in the intervention group were not statisticly significant than before (P＞0.05), and the serum levels of Alb, PA and VC were significantly higher than those in the routine group(P＜0.05).The incidence of complications in the in the intervention group was lower than that in the routine group (P＜0.05), and the length of hospital stay and hospitalization was shorter than that in the routine group (P＜0.05).Conclusion: Individualized enteral nutritional support can significantly improve nutritional status, reduce inflammation reactions and GI complications in severe stroke patients, which is worthy of clinical use.

The incidence of severe acute pancreatitis in children is lower.But it has abrupt onset and atypical symptoms.The fatality rate is relatively high.This paper will review the progress on the diagnosis and treatment of severe acute pancreatitis.

Congresses as Topic ; Humans ; Sleep Apnea, Obstructive

This paper explored the educational reform of dispensing Chinese drugherbs. The education reform included implementing the project of teaching methods, implementing modern experimental methods, cultivating comprehensive quality of students, training students' creative thinking, and stimulating the initiative of students. All these strategies could improve the quality of teaching and make students' comprehensive abilities meet the demand.

To set up a new method of detecting bacterial number in situ,NADH fluorescence method based on the fluorescent characteristic of NADH was used.When the concentration of NADH ranged from 10 nmol/L to 0.2 mmol/L,its concentration had a good line relationship to the fluorescence intensity(R2= 0.9905).Separating bacterial cells by centrifugation and extracting NADH with hot Tris-HCl buffer,the re-sult of bacterial count detected with NADH standard plot was 1?104 CFU/mL in an hour.In summary,NADH fluorescence method is rapid,sensitive,simple and reliable to detect total bacterial number.There-fore,the method can be widely applied in the field of food sanitation and safety,environment detection and so on.

To observe the incidence of high intraocular pressure ( lOP ) and its risk factors in the early stage (within 7d) after 23 - gauge sutureless microincisional vitrectomy.
●METHODS: Retrospective case series study. Totally 98 patients ( 98 eyes ) who had undergone 23 - gauge sutureless microincisional vitrectomy were enrolled. lOP was measured by non-contact tonometry. High lOP was defined as an lOP > 25mmHg at any time within 7d after surgery. The influence of age, sex, side of operation, course of disease, primary disease pre - operation, reoperation, surgical options, type of tamponade, status of lens, surgical time on postoperative high lOP were analyzed.
● RESULTS: High lOP was found in 33 eyes ( 33. 7%) within 7d after surgery. High lOP was found in 8 eyes on postoperative 1d, the incidence was 24. 2% (8 / 33), and 25 eyes was found on postoperative 3d, the incidence was 75. 8% (25 / 33). The incidence of high lOP of male and female were 32. 8% ( 20 / 61 ) and 35. 1% ( 13 / 37 ) respectively. The incidence of high lOP of right and left eye were 36. 8% (21 / 57) and 29. 3% (12 / 41) respectively. There were no significant differences comparing age (Z =-0. 22), sex( χ2 = 0. 057), side of operation( χ2 = 0. 612), course of disease (Z= -0.079) and surgical time (Z= -0. 553) between patients with high lOP and those without it (P>0. 05). Similarly, no statistical significance was found between eyes with gas tamponade and silicone oil tamponade (χ2 = 1. 04), traumatic eyes and non traumatic eyes(χ2 = 0. 044), and retinal detachment eyes and without retinal detachment eyes ( χ2 = 2. 282, P > 0. 05 ). The incidence of high lOP in eyes with several operations was higher than that in eyes with the first operation ( χ2 =5. 211,P< 0. 05), in eyes with combined operations eyes was significantly higher than that in eyes with pure vitrectomy (χ2 = 4. 57,P< 0. 05), and in eyes with aphakic eye was higher than that in eyes with phakic eyes ( χ2 =4. 224,P<0. 05).
●CONCLUSlON:High lOP occurs commonly in 3d after 23- gauge sutureless microincisional vitrectomy. The risk factors of high lOP are reoperation, combined operations and aphakic eye.

Animals ; Blood Glucose ; Diet, High-Fat ; Ghrelin ; blood ; Lipids ; blood ; Physical Conditioning, Animal ; Rats

Child ; Humans ; Inflammatory Bowel Diseases ; etiology ; pathology

Since the oral rehydration salts solution(ORS) has been used to treat acute diarrhea of children, there are different kinds of ORS in order to satisfy the need of clinical work. They are the initial WHO-ORS, other substances-supplemented ORS and the reduced osmolarity ORS, each of which has their own efficiency and advantage.