To observe the effect of interlocking intramedallary nail transplantation on homonymy fracture of shaft of tibia and femur. Totally 34 patients with traumatic homonymy fractures of middle-inferior 1/3 segment femurs and middle-superior 1/3 segment tibias were selected from Henan Luoyang Orthopedics Hospital from November 2000 to December 2005. Ti-alloy interlocking intramedallary nail made in Wujin Ter-factory of Jiangsu province was applied. To select the fron-outside patella opening approach, a needle was thread to femur and tibia step by step. Follow-up was performed at least 6 months after operation. The fractures of 34 patients got dissection positioning and synostosis. Functional evaluation of postoperative knee joint was good. Inserted bone nails were fixed, and there were no bad events such as cinched, breakdown nails and infections or side effects. It indicated that the modus operandi is simple and satisfactory to select the fron-outside patella opening approach and interlocking intramedallary nail to treat homonymy fracture of shaft of tibia and femur.

Objective To evaluate the value of multiplanar reconstruction (MPR) of spiral CT in diagnosing and staging of cervical cancer .Methods SCT scan with a 3～5 mm collimation thickness and MPR in 1.5～2.5 mm reconstruction interval were performed in 20 patients with cervical cancer , confirmed by biopsy pathology. Results The sagittal imaging could show entirely the growing character of a carcinomal mass;the coronal and transverse imagings showed clearly parametrial invasion and pelvic lymph node metastases. Conclusion MPR images in the pelvic could show entirely the character of growing and invasion of a carcinomal mass. It is an important value in dignosing and staging of cervical cancer.

We report a rare case of pleomorphic adenoma arising from the nasal septum. A 37-year-old woman presented with a 1-year-history of right-sided occasional epistaxis. Computed tomographic scans revealed an oval mass in the right nasal cavity. The tumor was removed endoscopically with endonasal approach. The microscopic finding showed numbers of myoepithelial cells and duct-like structures consisting of loose myxoid stroma. This lesion had histological characteristics of a pleomorphic adenoma, and this was confirmed by immunohistochemical expression of cytokeratin, S-100 protein and SMA. Her post-operative course was uneventful, and she is currently free from the disease 1.5 years after surgery.
Actins ; metabolism ; Adenoma, Pleomorphic ; diagnosis ; surgery ; Adult ; Endoscopy ; Epistaxis ; Epithelial Cells ; Female ; Humans ; Keratins ; metabolism ; Nasal Septum ; pathology ; Nose Neoplasms ; diagnosis ; surgery ; S100 Proteins ; metabolism

Objective To explore the evidence for the morphologic comparability between the meridians and interstitial connective tissues in the lower limb with auto-marked and 3D-Rebuilding technology. Methods First,the muscles were extracted and noise was eliminated;With edge detection a convex hull of the muscles edge was found;The interstitial connective tissue points were marked in the cross section images of left leg from the digital dataset of Virtual Chinese Human-Male1(VCH-M1).Finally,the auto-marked images were reconstructed with 3D-Doctor. Results The reconstructed meridians in the lower limb auto-marked with computer procedures tallied basically with the meridians and collaterals recorded in Traditional Chinese Medicine(TCM) illustrative graphics.Conclusion Having the advantages of digital anatomic study on acupuncture points earlier and mature 3D-Rebuilding techniques,the department of Anatomy of South Medical University cooperated with the Department of Scientific Calculation and Computer Application of Sun Yat-sen University to devise a set of computer programs which could be used to AutoMark interstitial connective tissues in Virtual Human lower limbs and then reconstruct virtual fascia meridians which corresponded with the TCM records.This method avoided man-made error to a certain degree in the process of recognizing and rebuilding of fascias.The study is significant to explore the morphologic comparability between the meridians and collaterals and interstitial connective tissues in human lower limb.

Objective:In order to understand the learning results and the medical students' attitude to study better,we took effective measures to better improve the quality of education.Methods Questionnaire were taken to inquire students' professional thinking and the attitude of study and the SPSS software was used to analyze the learning results.Results:Students had the problems of"four insufficiency and a pressure"during the learning procedure,and learning results of medical students showed big differences in different majors,different semesters and different genders.Conclusion:Our college should take necessary measures in enhancing education to regulate the professional thinking and the medical students' attitude of study.Our college teachers should get the total message and handle the students' learning results and give every student individual education.Meanwhile,we should pay more attention to letting students find the ways of obtaining necessary information and effective learning methods.

BACKGROUND:Absorbable bio-glass injection composed by bio-glass and calcium phosphate bone cement can be tightly combined with bone tissue by a strong chemical bond to improve the stability of the bone-implant interface.
OBJECTIVE:To investigate the effect of absorbable bio-glass injection to support the vertebral body of osteoporosis mice and its mechanism of osteogenic induction.
METHODS:Osteoporosis models were prepared in 30 female Sprague-Dawley rats undergoing bilateral ovariectomy. After modeling, model rats were randomly divided into three groups, and given polymethylmethacrylate, injectable calcium phosphate bone cement and absorbable bio-glass injection into L3-5 vertebral bone defects, respectively. L3-5 segments were removed at 12 weeks after implantation to detect the biomechanical and degradation properties, levels of calcium and phosphate, alkaline phosphatase activity, bone mineral density, levels of bone morphogenetic protein 2 and transforming growth factor β, as wel as histological observation.
RESULTS AND CONCLUSION: In the absorbable bio-glass injection group, the degradation properties, compressive strength, surface hydroxyapatite deposition amount and bone mineral density were significantly higher than those in the other two groups(P< 0.05); trabecular bone relative volume, thickness and number were significant higher than those in the other two groups (P < 0.05); serum levels of calcium, alkaline phosphatase, bone morphogenetic protein 2 and transforming growth factor β were significantly higher than those in the other two groups(P < 0.05); but the level of serum phosphate was lower than that in the other two groups(P < 0.05). These results show that the absorbable bio-glass injection can enhance the support for the osteoporotic vertebral body and induce osteogenesis, probably by increasing bone morphogenetic protein 2 and transforming growth factor β levels.

BACKGROUND:The mechanical performance and cytocompatibility of polylactic acid-polyethylene glycol (PLA-PEG) scaffold is poor via long-term experiments; therefore, other materials are added to the scaffold in order to improve its biological activity and mechanical properties. OBJECTIVE:To investigate the preparation process of carbon fiber (CF)-PLA-PEG scaffold, and to test its performance. METHODS:Modified CF-PLA-PEG composite scaffolds were prepared using solution injection-particle leaching method. Ultra-structure, porosity, water-absorbing quality, degradation rate and mechanical properties of CF-PLA-PEG composite scaffolds and PLA-PEG scaffolds were compared. Rat osteoblasts were co-cultured with these two kinds of scaffoldsin vitro, and the cel adhesion rate was detected by precipitation method after 12 hours. Cel counting was detected using MTT method to determine the cel proliferation at culture days 1, 3, 5, 7, 9. RESULTS AND CONCLUSION:The surface structure of PLA-PEG distributed uniformly, and the aperture was (404.0±10.5) μm. The carbon fiber surface of modified CF-PLA-PEG composite scaffolds had a lot of longitudinal grooves, the surface structure also distributed uniformly, and the aperture was (433.0±3.0) μm. Difference in scaffold aperture was of significance between the two groups (P < 0.05). The porosity, water-absorbing quality, elasticity modulus, compressive strength, degradation rate, cel adhesion rate and cel growth rate of CF-PLA-PEG were better than those of PLA-PEG (P < 0.05). These results show that the CF-PLA-PEG scaffolds have good mechanical property and cel compatibility.

Objective To screen and analyze microRNA (miRNA) molecules associated with immune clearance in patients with chronic hepatitis B (CHB).Methods Twelve hospitalized CHB patients and 9 healthy individuals from Taizhou People's Hospital during June 2011 and August 2012 were enrolled.miRCURYTM chip was used to identify differential expression patterns of miRNA.The target genes of differentially expressed miRNA molecules were predicted by TargetScan,miRBase and miRanda databases,and their molecular pathways and functions were analyzed with bioinformatic methods.Results Compared with healthy individuals,52 differentially expressed miRNA molecules were identified in PBMCs of CHB patients,including 33 up-regulated and 19 down-regulated ones.With TargetScan,miRBase and miRanda databases,354 target genes were predicted in up-regulated miRNA molecules,and 1 935 target genes were predicted in down-regulated miRNA molecules.Gene ontology (GO) and Pathway analysis showed that several molecular pathways might be affected by up or down-regulated miRNA molecules.miRNA-mRNA network analysis showed that some target genes might be regulated by miRNA molecules like hsa-miR-520d-5p,hsa-miR-106a-Sp,hsa-miR-30a-5p and hsa-miR-29b-3p,and constituted a complex molecular network.Conclusion There are several miRNA molecules with abnormal expressions in CHB patients,which may be involved in immune clearance through the regulation of target genes and molecular pathways.

Objective To investigate the effect of different dose of dexmedetomidine for pre-venting the visceral traction reaction during caesarean section under spinal anesthesia.Methods Two hundred ASA physical status Ⅰ-Ⅱ patients,full-term primiparas scheduled for elective cesarean sec-tion were enrolled in this randomized,placebo-controlled study.Patients were randomly allocated to receive dexmedetomidine in four groups:group C (0.9% normal saline),group D1(dexmedetomidine 0.6 μg/kg),group D2(dexmedetomidine 0.8 μg/kg),group D3 (dexmedetomidine 1.0 μg/kg),50 cases in each group.Two minutes after cutting umbilical,groups D1,D2,D3 received an intravenous infusion single-dose of 0.6,0.8,1.0 μg/kg dexmedetomidine respectively for 10 minutes.Group C was injected the same dose of saline.Maternal blood pressure (BP),heart rate (HR),Ramsay scores,visceral traction reaction conditions were recorded at three points:before iv (T0 ),10 min af-ter iv (T1 )and abdominal exploration (T2 ).Results As for the inhibition of visceral traction pain, groups D2 and D3 were obviously better than groups D1 and C,and there was no statistically signifi-cant difference between groups D2 and D3 at T2 (P <0.05 ).The effects of slowing down heart rate and increasing blood pressure were weaker in group D2 than that in group D3 at T2 .Ramsay scores were higher in groups D2 and D3 than in groups D1 and C (P <0.05),and there was no significant difference between groups D2 and D3 at T2 .Conclusion Administration of dexmedetomidine 0.8 μg/kg could alleviate the visceral traction reaction and had little effect on maternal hemodynamics.

To investigate the effect of C/EBP βon the neuroinflammation.Recently,neuroinflamma-tion research mainly focused on C/EBP β,more and more researches indicated that C/EBP βmay play an important role in neurodegenerative diseases.We searched the published papers in PubMed,CNKI,and Wan Fang date bases in June 2015.The key words were used asCCAAT enhancer binding protein beta,Neu-ron,Neuroinflammationand so on.According to the inclusion and exclusion criteria, we summaried and analyzed the literatures.Eventually,42 articles were adapted,most of which were foused on the functions and mechanisms of C/EBP βin neuroinflammation.C/EBP βparticipate in multiple signaling pathways of neu-roinflammation,and the regulation mechanism is complicated.Abrogation of C/EBPβexpression or its down-regulation by gene regulation may may serve as a therapeutic target to attenuate deleterious effects in neural tissue and ultimately prevent the development of neurodegenerative disorders.