Humans ; Podocytes ; physiology ; Proteinuria ; physiopathology

The production conditions of glutathione with shaking flask fermentation by the mutant Saccharomyces J-X25, a methionine-defected strain were studied, and the optimum culture conditions are as follows: initial pH6. 0, temperature 30℃, 100ml in 500ml flask, the inoculum size 10% and agitation rate 220r/min. The emphasis was on the stimulating effect on the cells by dioxogen and the sodium lactate as surfactant. Both of which were added at the logarithmic phase of fermentation, and the GSH production was up to 0. 253g/L , 52% higher than the control without the additions. Compared with the production of GSH initial strain that by the mutant in optimum conditions was increased by 79%.

Aim The effect and mechanism of human placental extract(HPE) on the lipoprotein-cholesterol metabolism, peroxidation and the function of platelet aggregation in hyperlipaemia rats were abserved.Methods Wistar rat with hyperlipaemia models were given each HPE 0.4 ml (100 g)-1?d-1 through lavage for 12 days.The serum levels of TG,TC,LDL-C,HDL-C and HDL2-C in its subgroup were measured.The activies of LPO and SOD in both blood and liver tissue were determined .The effect of HPE on lipidosis of liver were abserved by fat dyeing.The levels of 6-keto-PGF1?,TXB2 in plasma and maximum platelet aggregation rate were measured by ELASA. Result The levels of HDL-C and HDL2-C were increased (P

Objective To explore the best treatment program for neurogenic dysphagia (ND) by observing the efficacy of three different rehabilitation programs.Methods Eighty-two dysphagic patients were randomly divi-ded into a swallowing training group (which received mouth,facial,and tongue function training along with cold sti-mulation of the pharynx,Mendelson's swallowing training,and swallowing behavior coordination training) and a group which received neuromuscular electrical stimulation (NMES) only.Low-frequency NMES was applied at the location of the swallowing obstacle using a biphase square wave 700ms long at 30-80 Hz with a current intensity of 0-15 mA.There was also a combined treatments group which received swallowing behavior therapy combined with NMES.All three groups were treated for 30 min daily for 20 days.Videofluoroscopy (VFSS) was conducted before and after treatment to assess swallowing function.Results After 20 days of treatment,swallowing function had improved in all three groups.The effectiveness rate was 73% in the swallowing training group,83% in the NMES group and 94% in the combined treatment group.The average VFSS score in the combined therapy group was significantly higher than that of either the swallowing training or NMES group.Conclusions NMES combined with swallowing behavior the-rapy can greatly improve swallowing function in patients with neurogenic dysphagia,especially those with pseudosupranuclear paralysis.

OBJECTIVE:To analyze the manifestations and characteristics of adverse drug reactions (ADR) induced by Traditional Chinese medicine.METHODS:85 ADR reports collected from the spontaneous reporting system in our hospital from 2005 to 2008 were analyzed retrospectively.RESULTS:ADR almost involved all the body system and 31.63% were nervous system.Main dosage form inducing ADR was injection (51.76%).CONCLUSION:ADR induced by Traditional Chinese Medicine can not be disregarded.Clinical pharmacists should pay more attention to the drug therapy by intravenous administration to reduce the incidence of ADR.

Objective To study the anti-fatigue effects of enzymatic hydrolyzed protein from Pinctada martensii.Methods Swimming time,contents of liver glycogen and serum urea nitrogen after swimming were determined.Results Three dosages of EPA were all able to prolong swimming time and decrease liver glycogen consumption and serum urea nitrogen content after exhausted swimming in various degree.Conclusion The EAP has evident anti-fatigue effects.

Objective To study the effect of liver transplantation using right liver lobe Ⅴ and Ⅷ segments with middle hepatic vein(MHV) and without main MHV on the regeneration of liver transplantation. Methods 25 pair of donors and receptors of liver transplantation were divided in to two groups:with M HV group (group A, n= 14) and without main MHV (group B, n=11). The volumes of Ⅴ and Ⅷ segments of liver were measured in donors presurgicaUy,half month and 3 month in receptors postsurgically, the rates of regeneration of the Ⅴ and Ⅷ segment were calculated half month and 3 months postsurgically and compared between two groups. Results In group A,the regenerate rates of the Ⅴ,Ⅷ segment were 0.360±0.043 and 0.853±0.059 half month after surgery,0.253±0.043 and 0.708±0.059 three months after surgery respectively. In group B, the regenerate rates of the Ⅴ,Ⅷ segment were 0.306±0.049 and 0.815±0.066, respectively half month after graft, 0.161±0.049 and 0.627±0.066, respectively three months after surgery. There were no statistically significant differences in regenerate rates of the Ⅴ, Ⅷ segment of graft be-tween the two groups (P= 0.685 ,P>0.05 and P= 0.738, P>0.05). Conclusion The right lobe living donor liver transplantation, maintaining the MHV or reconstruct MHV tributaries without MHV has similar effects on the regeneration of Ⅴ,Ⅷ segment of graft.

Objective To observe the changes and relationships of glucose (Glu),insulin(Ins) ,insulin-like growth factor-1 (IGF-1) in newborn infant with hypixic-ischemic encephalopathy (HIE) and the protective role which Ins,IGF-1 worked on neuron.Methods HIE 50 cases,the quantity of IGF-1 in serum was detected by radioimmunoassay,the quantity of Ins in serum was detected by chemiluminescent immunoassay,the quantity of glucose in serum was detected by the instrument with type of Rokangquan TM accutive.The umbilical vein blood 2 mL were drawn from 20 cases of controls after birth.The indexes were detected by the same methods.Results 1.In acute period,the level of serum IGF-1 reduced obviously in newborn infants with HIE,and Glu increased obviously.There was a negative correlation between IGF-1 and Glu.The changes related with neonate complications.In rehabilited pe riod,the Glu get to normal,the IGF-1 was a little higher than that in acute period.but it still was lower than that of control group.There was negative correlation between IGF-1 and Glu.2.The level of Ins in serum of newborn infants with HIE had no obvious changes compairing with control group in acute period and rehabilited period.It had no relations with Glu,Ins.Conclusions It approves that IGF-1 and Glu in serum of newborn infants with HIE have obviously changed.This result will provide an academic base on treatment of HIE.

Objective To investigate the efficacy of oxygen uptake efficiency slope (OUES) on evaluation the cardiopulmonary function of patients with chronic obstructive pulmonary disease (COPD). Methods The cardiopulmonary function of 54 stable COPD patients with the cardiopulmonary function of Ⅱ~Ⅳ were evaluated, following a symptom-limited Steep protocol with simultaneous respiratory gas measurement,they were performed exercise tests on a treadmill, simultaneously the oxygen uptake (VO2), carbon dioxide production (VCO2),peak oxygen uptake (VO2peak), minute ventilation (VE), and respiratory gas exchange rate (RER) were measured. OUES was derived from the relation between VO2 and VE during incremental exercise and was determined by VO2=algVE+b, where a=OUES, to measure anaerobic threshold (VAT) meanwhile. Results OUES correlated with the VO2peak (P<0.001). 75% OUES, 90% OUES and 100% OUES were not significantly different (F=0.239, P=0.830). Conclusion OUES can respond the cardiopulmonary function in patients with COPD, 75% OUES from sub-maximal exercise can be an index for cardiopulmonary function.

Child ; Humans ; Lupus Nephritis ; complications ; Male ; Nephritis, Interstitial ; etiology ; Prognosis