1.Application of qualitative interviews in inheritance research of famous old traditional Chinese medicine doctors: ideas and experience.
Jing LUO ; Chang-geng FU ; Hao XU
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(4):492-496
The inheritance of famous old traditional Chinese medicine (TCM) doctors plays an essential role in the fields of TCM research. Qualitative interviews allow for subjectivity and individuality within clinical experience as well as academic ideas of doctors, making it a potential appropriate research method for inheritance of famous old TCM doctors. We summarized current situations of inheritance research on famous old TCM doctors, and then discussed the feasibility of applying qualitative interviews in inheritance of famous old TCM doctors. By combining our experience in research on inheritance of famous old TCM doctors, we gave some advice on study design, interview implementation, data transcription and analyses , and report writing, providing a reference for further relevant research.
Humans
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Interviews as Topic
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Medicine, Chinese Traditional
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Physicians
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Research Design
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Writing
2.Establishment of a real time quantitative reverse-transcription PCR method for detection of hepatitis E virus based on standard plasmid
Jing WANG ; Jiabao GENG ; Qian HE ; Zhidong HU ; Hongwei FU
Chinese Journal of Microbiology and Immunology 2017;37(2):147-154
Objective To establish a real-time quantitative reverse-transcription PCR ( qRT-PCR) method for detection of hepatitis E virus ( HEV) of different genotypes based on standard HEV DNA plasmid in order to promote its application in clinical laboratory. Methods Specific primers and probe of HEV were designed based on the conserved open reading frame 3 (ORF3) regions. HEV DNA plasmids were construc-ted and 10-fold serial dilutions of the plasmids were prepared and used as standards to establish one-step qRT-PCR. The established method was compared with HEV antigen, antibody and RT-nPCR assays. Some positive samples were sequenced and analyzed by evolutionary tree. Results The one-step qRT-PCR meth-od for HEV detection in serum or feces samples was successfully establish. It could reach a sensitivity of 25 copies/test and 77. 8% of its results were consistent with those by HEV antigen assay. Nine patients were infected with HEV of genotypes 4a, 4d or 4n as indicated by evolutionary tree. Conclusion The HEV qRT-PCR method based on its standard plasmid is successfully established, which paves the way for commercial-ization of clinical applications.
3.CT findings of lymphofollicular thymic hyperplasia in adult myasthenia gravis
Fu-Geng LIU ; Jia-Hu WEI ; Ji-Shu PAN ; Cheng ZHOU ; Qi-Hang CHEN ; Jing-Ying YU ; Guo-Geng WU ; Xian-Hao XU ;
Chinese Journal of Radiology 2001;0(08):-
Objective To evaluate the CT findings of lymphofollicular thymic hyperplasia in adult myasthenia gravis (MG).Methods The CT findings of thymus area of 134 adult patients with lymphofolficular thymic hyperplasia in MG were reviewed,all of them with surgically and histologically proven diagnosis,and compared with the CT findings of 165 normal subjects.Results In the group of patient,CT showed enlargement of thymus in 31 patients,5 patients had nodule or mass(<3 cm);thus 36 cases(26.9%)can confirmed diagnose by CT with thymic hyperplasia.CT showed 2 masses(>3 em) and 9 patients(6.7%)had normal size thymus with soft-tissue density,it can considered with thymic hyperplasia.The spotty or streak shadow showed in other patients,though it could not be certain diagnosed as thymic hyperplasia,but could not be except it.The thymus area tissue complete replacement by fatty density were not found in patient group.The CT findings of patients had marked difference when compared with group of normal subjects(P<0.01),except the spotty or streak shadows.Conclusion CT scan is an important method in diagnosing thymic lymphofollicular hyperplasia of MG in adult.
4.Changes in serum insulin-like growth factor-1 and insulin-like growth factor-binding protein-3, and their significance in children with left-to-right shunt congenital heart disease associated with heart failure.
Jing PENG ; Jia FU ; Shu-Zhen DENG ; Rui-Geng WANG ; Ling LIU ; Dong-Ming SUN ; Kun XIA
Chinese Journal of Contemporary Pediatrics 2013;15(4):277-280
OBJECTIVETo investigate changes in serum insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-binding protein-3 (IGFBP-3) and their significance in children with left-to-right shunt congenital heart disease (CHD) associated with heart failure (HF).
METHODSTwenty healthy children (control group), 20 children with HF, without basic heart disease (HF group), 20 children with left-to-right shunt CHD, without HF (CHD group), and 30 children with left-to-right shunt CHD associated with HF (CHD+HF group) were included in the study. These groups were compared in terms of serum IGF-1 and IGFBP-3 levels. According to the New York Heart Association (NYHA) Functional Classification, the CHD+HF group was further divided into NYHA-II, NYHA-III and NYHA-IV subgroups and the subgroups were compared in terms of serum IGF-1, IGFBP-3, and cardiac troponin I (cTnI) levels. The correlation of serum IGF-1 and IGFBP-3 levels with serum cTnI level in the CHD+HF group was analyzed.
RESULTSThe CHD group showed decreased serum IGF-1 and IGFBP-3 levels compared with the control group (P<0.01). The CHD+HF group showed a significantly decreased serum IGF-1 level compared with the control group (P<0.01) and CHD group (P<0.05). The HF group had significantly increased serum IGF-1 and IGFBP-3 levels compared with other groups (P<0.01). The NYHA-II subgroup had the highest serum IGF-1 level and the NYHA-IV subgroup had the lowest serum IGF-1 level (P<0.01). In the CHD+HF group, serum IGF-1 and IGFBP-3 levels were negatively correlated with serum cTnI level (r=-0.692, P<0.05; r=-0.530, P<0.05).
CONCLUSIONSSerum IGF-1 level can be used as an objective condition evaluation indicator for CHD, and low serum IGF-1 level is a risk factor for HF. This also provides a clinical basis for treatment of HF using exogenous IGF-1.
Child, Preschool ; Female ; Heart Defects, Congenital ; blood ; Heart Failure ; blood ; Humans ; Infant ; Insulin-Like Growth Factor Binding Protein 3 ; blood ; Insulin-Like Growth Factor I ; analysis ; Male ; Troponin I ; blood
5.Closed reduction and percutaneous Kirschner-wire fixation for the treatment of supracondylar fractures in children: a report of 37 cases.
Nai-qiang WEI ; Fu-dou YIN ; Lin-lin ZHANG ; Jing-hui GENG ; Wei-chao SHENG
China Journal of Orthopaedics and Traumatology 2008;21(4):309-310
Bone Wires
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Child
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Child, Preschool
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External Fixators
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Female
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Fracture Fixation
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methods
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Humans
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Humeral Fractures
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surgery
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Male
6.First case of group X meningococcal disease in Hunan Province:case re-port and literature review
Ting-Xin YAO ; Fu-Rong LIU ; Geng-Ji XIAO ; Jing LIU
Chinese Journal of Infection Control 2023;22(12):1530-1533
The group X meningococcal disease is rare in China and developed countries.No related cases have been reported in Human Province.The disease progresses rapidly and leads to critical severity.Serious complica-tions may occur,if not treated actively.Group X Neisseria meningitidis(Nm X)vaccine has not yet obtained per-mission at present.This paper collects data on the symptoms,signs,auxiliary examinations,and treatment process of the first patient with severe group X meningococcal disease in Hunan Province,reviews relevant literatures,so as to improve clinicians'understanding on group X meningococcal disease,conduct early identification,diagnosis and treatment of the disease.
7.C-reactive Protein -717A>G and -286C>T>A Gene Polymorphism and Ischemic Stroke.
Yan LIU ; Pei-Liang GENG ; Fu-Qin YAN ; Tong CHEN ; Wei WANG ; Xu-Dong TANG ; Jing-Chen ZHENG ; Wei-Ping WU ; Zhen-Fu WANG
Chinese Medical Journal 2015;128(12):1666-1670
BACKGROUNDInflammation plays a pivotal role in the formation and progression of ischemic stroke. Recently, more and more epidemiological studies have focused on the association between C-reactive protein (CRP) -717A > G and -286C > T > A genetic polymorphisms and ischemic stroke. However, the findings of these researches are not conclusive.
METHODSWe performed a meta-analysis to determine whether these two polymorphisms are associated with the risk of ischemic stroke. Eligible studies were identified from the database of PubMed, Medline, Embase, Web of Science, CNKI, Weipu, and Wanfang. We calculated odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of the association.
RESULTSFour articles were included in our study, including 1926 cases and 2678 controls for -717A > G polymorphism, 652 cases and 1103 controls for -286C > T > A polymorphism. The results of meta-analysis showed that single nucleotide polymorphism (SNP) -717A > G was not significantly associated with the risk of ischemic stroke (GG vs. AA, OR = 1.12, 95% CI = 0.83-1.50, P = 0.207; GG + GA vs. AA, OR = 1.04, 95% CI = 0.93-1.17, P = 0.533; GG vs. GA + AA, OR = 1.10, 95% CI = 0.82-1.47, P = 0.220). Meta-analysis of SNP - 286C > T > A also demonstrated no statistical evidence of a significant association with the risk of ischemic stroke (AA vs. CC, OR = 0.86, 95% CI = 0.59-1.25, P = 0.348; AA vs. CC, OR = 0.92, 95% CI = 0.80-1.06, P = 0.609; AA vs. CC, OR = 0.89, 95% CI = 0.62-1.30, P = 0.374).
CONCLUSIONSThis meta-analysis demonstrated little evidence to support a role of CRP gene -717A > G, -286C > T > A polymorphisms in ischemic stroke predisposition. However, to draw comprehensive and more reliable conclusions, further larger studies are needed to validate the association between CRP gene polymorphisms and ischemic stroke in various ethnic groups.
Alleles ; Brain Ischemia ; genetics ; C-Reactive Protein ; genetics ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Polymorphism, Single Nucleotide ; genetics ; Stroke ; epidemiology ; genetics
8.Treatment of acne vulgaris with auricular acupoint pricking-bloodletting plus auricular point sticking therapy: a randomized controlled study
Ya-Jing SONG ; Xi-Sheng FAN ; Meng-Yun LI ; Jie ZHANG ; Jing-Ran GENG ; Xiao LIANG ; Jun-Cha ZHANG ; Xiao-Qi ZHANG ; Shu-Bo ZHOU ; Nan WANG ; Jia-Xu ZHANG ; Xiao-Dan SONG ; Yan-Fen SHE ; Fu-Qing ZHANG
Journal of Acupuncture and Tuina Science 2019;17(3):196-202
Objective: To observe the clinical efficacy of auricular point pricking-bloodletting plus auricular point sticking therapy for acne vulgaris. Methods: A total of 66 patients with acne vulgaris were randomized into an observation group and a control group by the random number table, with 33 cases in each group. The observation group was treated with auricular point pricking-bloodletting plus auricular point sticking therapy, and the control group was treated only with auricular point sticking therapy. The treatments of both groups were performed twice a week, 4 weeks as a course of treatment, for 3 courses in total. The scores of skin lesions and dermatology life quality index (DLQI) scores were recorded before and after treatment to assess the clinical efficacy. Results: During the trial, there were 3 cases of drop-out both in the observation group and the control group. After 3 courses of treatment, the total effective rate of the observation group was 96.7%, while that of the control group was 76.7%. The difference between the two groups was statistically significant (P<0.05). The intra-group comparison showed that the scores of skin lesion and DLQI were both decreased with the increase of treatment times, that was, the scores were lower than those at the previous time point (allP<0.05). After 1, 2, and 3 courses of treatment, the scores of skin lesion and DLQI of both groups were statistically different from those of the same group before treatment (allP<0.05). At every time point during the treatment, the scores of skin lesion and DLQI of the observation group were lower than those of the control group, and the differences between the two groups were statistically significant (all P<0.05). Conclusion: Auricular point pricking-bloodletting plus auricular point sticking has a better curative effect than auricular point sticking therapy alone in the treatment of acne vulgaris, and has a time-effect correlation.
9.The role of diaphgram fatigue in the death from hanging with bound upper limbs of rabbits.
Fu-Xue JIANG ; Han-Lan JING ; Gui-Sheng OU ; Xin-Biao LIAO ; Yi-Jun ZHANG ; Yan-Geng YU ; Jian-Ding CHENG ; Zhao-Hui LI ; Xiao-Ran GUO
Journal of Forensic Medicine 2006;22(3):165-167
OBJECTIVE:
To explore the role of diaphgram fatigue in the death from hanging with bound upper limbs of rabbits.
METHODS:
Rabbits were hanged with upper limbs bound, then the data of EMGdi were gathered
RESULTS:
By analyzing power spectral of EMGdi in experiment, we compare the ratio change of H/L between pre-experiment and post-experiment. There is a significance decrease of the ratio of H/L, so it indicates that diaphgram fatigue does exist.
CONCLUSION
Diaphgram fatigue plays an important role in the death from hanging with limbs of rabbits bound.
Animals
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Asphyxia/physiopathology*
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Cause of Death
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Diaphragm/physiopathology*
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Electromyography
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Female
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Forensic Medicine
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Male
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Muscle Fatigue
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Rabbits
10.Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae.
Hong-jun YANG ; Mei-hong FU ; Zi-lun WU ; Ri-xing LIANG ; Lu-qi HUANG ; Jing FANG ; Geng LI ; Ying CAO
China Journal of Chinese Materia Medica 2006;31(13):1091-1093
OBJECTIVETo study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.
METHODObserve the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope.
RESULTThe weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate.
CONCLUSIONAqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.
Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; Bilirubin ; blood ; Drugs, Chinese Herbal ; isolation & purification ; toxicity ; Female ; Fruit ; chemistry ; Gardenia ; chemistry ; Iridoids ; isolation & purification ; toxicity ; Liver ; pathology ; Male ; Organ Size ; drug effects ; Plants, Medicinal ; chemistry ; Pyrans ; isolation & purification ; toxicity ; Random Allocation ; Rats ; Rats, Sprague-Dawley